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Protein

Short transient receptor potential channel 4

Gene

Trpc4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Has also been shown to be calcium-selective (By similarity). May also be activated by intracellular calcium store depletion. Trpc4 deficient mice lack a store-operated calcium entry in endothelial cells.By similarity1 Publication

GO - Molecular functioni

  • beta-catenin binding Source: MGI
  • cadherin binding Source: MGI
  • calcium channel activity Source: MGI
  • inositol 1,4,5 trisphosphate binding Source: BHF-UCL
  • store-operated calcium channel activity Source: MGI

GO - Biological processi

  • calcium ion import Source: MGI
  • calcium ion transport Source: MGI
  • gamma-aminobutyric acid secretion Source: MGI
  • manganese ion transport Source: GO_Central
  • oligodendrocyte differentiation Source: MGI
  • regulation of calcium ion transport Source: MGI
  • regulation of cytosolic calcium ion concentration Source: GO_Central

Keywordsi

Molecular functionCalcium channel, Ion channel
Biological processCalcium transport, Ion transport, Transport
LigandCalcium

Enzyme and pathway databases

ReactomeiR-MMU-3295583 TRP channels

Names & Taxonomyi

Protein namesi
Recommended name:
Short transient receptor potential channel 4
Short name:
TrpC4
Alternative name(s):
Capacitative calcium entry channel Trp4
Receptor-activated cation channel TRP4
Gene namesi
Name:Trpc4
Synonyms:Trrp4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:109525 Trpc4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 329CytoplasmicSequence analysisAdd BLAST329
Transmembranei330 – 350HelicalSequence analysisAdd BLAST21
Topological domaini351 – 362ExtracellularSequence analysisAdd BLAST12
Transmembranei363 – 383HelicalSequence analysisAdd BLAST21
Topological domaini384 – 436CytoplasmicSequence analysisAdd BLAST53
Transmembranei437 – 457HelicalSequence analysisAdd BLAST21
Topological domaini458 – 469ExtracellularSequence analysisAdd BLAST12
Transmembranei470 – 490HelicalSequence analysisAdd BLAST21
Topological domaini491 – 511CytoplasmicSequence analysisAdd BLAST21
Transmembranei512 – 532HelicalSequence analysisAdd BLAST21
Topological domaini533 – 599ExtracellularSequence analysisAdd BLAST67
Transmembranei600 – 620HelicalSequence analysisAdd BLAST21
Topological domaini621 – 974CytoplasmicSequence analysisAdd BLAST354

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1741219
GuidetoPHARMACOLOGYi489

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002153151 – 974Short transient receptor potential channel 4Add BLAST974

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei956Phosphotyrosine; by FYNBy similarity1
Modified residuei969Phosphotyrosine; by FYNBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9QUQ5
PRIDEiQ9QUQ5

PTM databases

iPTMnetiQ9QUQ5
PhosphoSitePlusiQ9QUQ5

Expressioni

Tissue specificityi

Abundantly expressed in brain (hippocampal CA1 pyramidal neurons, dentate gyrus granule cells, and cerebral cortical neurons, and in the septal nuclei and the mitral layer of olfactory bulb). Lower levels are detected in other tissues.

Gene expression databases

BgeeiENSMUSG00000027748
ExpressionAtlasiQ9QUQ5 baseline and differential
GenevisibleiQ9QUQ5 MM

Interactioni

Subunit structurei

Homotetramer. Heterotetramer with TRPC1 and/or TRPC5 (By similarity). Isoform alpha but not isoform beta associates with inositol 1,4,5-trisphosphate receptor (ITPR) (By similarity). Interacts with NHERF. Interacts with MX1 and RNF24 (By similarity). Interacts (via CIRB domain) with SESTD1 (via the spectrin 1 repeat) and SPTBN5 (via C-terminus) (By similarity). Interacts with CDH5 and CTNNB1 (By similarity). Interacts (via protein 4.1-binding domain) with EPB41L2 (By similarity). Interacts with TRPC4AP.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204330, 6 interactors
CORUMiQ9QUQ5
DIPiDIP-40848N
IntActiQ9QUQ5, 6 interactors
MINTiQ9QUQ5
STRINGi10090.ENSMUSP00000029311

Chemistry databases

BindingDBiQ9QUQ5

Structurei

Secondary structure

1974
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi17 – 19Combined sources3
Helixi31 – 39Combined sources9
Turni40 – 43Combined sources4
Helixi45 – 53Combined sources9
Turni54 – 56Combined sources3
Beta strandi68 – 70Combined sources3
Beta strandi73 – 75Combined sources3
Helixi76 – 79Combined sources4
Helixi83 – 91Combined sources9
Helixi99 – 105Combined sources7
Helixi111 – 116Combined sources6
Helixi145 – 151Combined sources7
Helixi155 – 162Combined sources8
Beta strandi167 – 169Combined sources3
Helixi190 – 203Combined sources14
Turni206 – 210Combined sources5
Helixi216 – 231Combined sources16
Helixi238 – 257Combined sources20
Helixi264 – 268Combined sources5
Helixi287 – 294Combined sources8
Turni298 – 300Combined sources3
Helixi305 – 314Combined sources10
Helixi321 – 323Combined sources3
Helixi326 – 338Combined sources13
Helixi340 – 345Combined sources6
Turni346 – 348Combined sources3
Beta strandi351 – 353Combined sources3
Turni354 – 360Combined sources7
Helixi362 – 383Combined sources22
Helixi401 – 422Combined sources22
Helixi427 – 431Combined sources5
Helixi433 – 456Combined sources24
Helixi473 – 489Combined sources17
Helixi491 – 498Combined sources8
Turni500 – 502Combined sources3
Helixi503 – 517Combined sources15
Helixi520 – 541Combined sources22
Beta strandi546 – 548Combined sources3
Beta strandi551 – 556Combined sources6
Beta strandi560 – 563Combined sources4
Helixi564 – 573Combined sources10
Turni574 – 578Combined sources5
Helixi581 – 584Combined sources4
Helixi591 – 609Combined sources19
Helixi612 – 627Combined sources16
Helixi635 – 644Combined sources10
Beta strandi646 – 650Combined sources5
Helixi655 – 657Combined sources3
Helixi693 – 724Combined sources32
Helixi733 – 754Combined sources22

3D structure databases

ProteinModelPortaliQ9QUQ5
SMRiQ9QUQ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati31 – 60ANK 1Add BLAST30
Repeati69 – 97ANK 2Add BLAST29
Repeati98 – 124ANK 3Add BLAST27
Repeati141 – 170ANK 4Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni87 – 172Multimerization domainAdd BLAST86
Regioni254 – 304Multimerization domainAdd BLAST51
Regioni615 – 974Binds to ITPR1, ITPR2 and ITPR3By similarityAdd BLAST360
Regioni972 – 974PDZ-binding domainBy similarity3

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili223 – 260Sequence analysisAdd BLAST38

Sequence similaritiesi

Keywords - Domaini

ANK repeat, Coiled coil, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3609 Eukaryota
ENOG410XQ0Y LUCA
GeneTreeiENSGT00760000119180
HOGENOMiHOG000020589
HOVERGENiHBG068337
InParanoidiQ9QUQ5
KOiK04967
OMAiCILVDHR
OrthoDBiEOG091G029I
PhylomeDBiQ9QUQ5
TreeFamiTF313147

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR005821 Ion_trans_dom
IPR004729 TRP_channel
IPR013555 TRP_dom
IPR005460 TRPC4_channel
IPR002153 TRPC_channel
PANTHERiPTHR10117 PTHR10117, 1 hit
PTHR10117:SF25 PTHR10117:SF25, 1 hit
PfamiView protein in Pfam
PF00520 Ion_trans, 1 hit
PF08344 TRP_2, 1 hit
PRINTSiPR01097 TRNSRECEPTRP
PR01645 TRPCHANNEL4
SMARTiView protein in SMART
SM00248 ANK, 2 hits
SUPFAMiSSF48403 SSF48403, 1 hit
TIGRFAMsiTIGR00870 trp, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: Q9QUQ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQFYYKRNV NAPYRDRIPL RIVRAESELS PSEKAYLNAV EKGDYASVKK
60 70 80 90 100
SLEEAEIYFK ININCIDPLG RTALLIAIEN ENLELIELLL SFNVYVGDAL
110 120 130 140 150
LHAIRKEVVG AVELLLNHKK PSGEKQVPPI LLDKQFSEFT PDITPIILAA
160 170 180 190 200
HTNNYEIIKL LVQKGVSVPR PHEVRCNCVE CVSSSDVDSL RHSRSRLNIY
210 220 230 240 250
KALASPSLIA LSSEDPFLTA FQLSWELQEL SKVENEFKSE YEELSRQCKQ
260 270 280 290 300
FAKDLLDQTR SSRELEIILN YRDDNSLIEE QSGNDLARLK LAIKYRQKEF
310 320 330 340 350
VAQPNCQQLL ASRWYDEFPG WRRRHWAVKM VTCFIIGLLF PVFSVCYLIA
360 370 380 390 400
PKSPLGLFIR KPFIKFICHT ASYLTFLFLL LLASQHIDRS DLNRQGPPPT
410 420 430 440 450
IVEWMILPWV LGFIWGEIKQ MWDGGLQDYI HDWWNLMDFV MNSLYLATIS
460 470 480 490 500
LKIVAFVKYS ALNPRESWDM WHPTLVAEAL FAIANIFSSL RLISLFTANS
510 520 530 540 550
HLGPLQISLG RMLLDILKFL FIYCLVLLAF ANGLNQLYFY YEETKGLSCK
560 570 580 590 600
GIRCEKQNNA FSTLFETLQS LFWSIFGLIN LYVTNVKAQH EFTEFVGATM
610 620 630 640 650
FGTYNVISLV VLLNMLIAMM NNSYQLIADH ADIEWKFART KLWMSYFEEG
660 670 680 690 700
GTLPTPFNVI PSPKSLWYLV KWIWTHLCKK KMRRKPESFG TIGRRAADNL
710 720 730 740 750
RRHHQYQEVM RNLVKRYVAA MIREAKTEEG LTEENVKELK QDISSFRFEV
760 770 780 790 800
LGLLRGSKLS TIQSANAASS ADSDEKSQSE GNGKDKRKNL SLFDLTTLIH
810 820 830 840 850
PRSAAIASER HNLSNGSALV VQEPPREKQR KVNFVADIKN FGLFHRRSKQ
860 870 880 890 900
NAAEQNANQI FSVSEEITRQ QAAGALERNI ELESKGLASR GDRSIPGLNE
910 920 930 940 950
QCVLVDHRER NTDTLGLQVG KRVCSTFKSE KVVVEDTVPI IPKEKHAHEE
960 970
DSSIDYDLSP TDTAAHEDYV TTRL
Length:974
Mass (Da):111,575
Last modified:May 1, 2000 - v1
Checksum:i2D0BB2F235F5F8D1
GO
Isoform Beta (identifier: Q9QUQ5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     781-864: Missing.

Show »
Length:890
Mass (Da):102,181
Checksum:i26901B5A81068694
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti780E → K in AAF01469 (PubMed:9512398).Curated1
Sequence conflicti890R → L in AAF01469 (PubMed:9512398).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006570781 – 864Missing in isoform Beta. 4 PublicationsAdd BLAST84

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011543 mRNA Translation: AAD10167.1
U50922 mRNA Translation: AAC05179.1
AF190646 mRNA Translation: AAF01469.1
U50921 mRNA Translation: AAC05178.1
AF019663 mRNA Translation: AAD10168.1
X90697 mRNA Translation: CAA62230.1
CCDSiCCDS17350.1 [Q9QUQ5-1]
CCDS79904.1 [Q9QUQ5-2]
PIRiS59128
RefSeqiNP_001240611.1, NM_001253682.1 [Q9QUQ5-2]
NP_058680.1, NM_016984.3 [Q9QUQ5-1]
XP_006501359.1, XM_006501296.3 [Q9QUQ5-1]
UniGeneiMm.10100
Mm.489865

Genome annotation databases

EnsembliENSMUST00000029311; ENSMUSP00000029311; ENSMUSG00000027748 [Q9QUQ5-1]
ENSMUST00000200048; ENSMUSP00000143593; ENSMUSG00000027748 [Q9QUQ5-2]
GeneIDi22066
KEGGimmu:22066
UCSCiuc008pfd.2 mouse [Q9QUQ5-1]
uc008pff.2 mouse [Q9QUQ5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTRPC4_MOUSE
AccessioniPrimary (citable) accession number: Q9QUQ5
Secondary accession number(s): Q62350, Q9QUQ9, Q9QZC0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: May 1, 2000
Last modified: July 18, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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