Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Toll-like receptor 2

Gene

Tlr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity) (PubMed:15690042). May also promote apoptosis in response to lipoproteins (By similarity). Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2:TLR6:CD14:CD36 in response to diacylated lipopeptides and TLR2:TLR1:CD14 in response to triacylated lipopeptides (By similarity). Recognizes M.tuberculosis major T-antigen EsxA (ESAT-6) which inhibits downstream MYD88-dependent signaling (PubMed:17486091). Acts as the major receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PhoS1 (pstS1), in conjunction with TLR1 and for some but not all lipoproteins CD14 and/or CD36. The lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed:19362712). Recombinant MPT83 from M.tuberculosis stimulates secretion of cytokines (TNF-alpha, IL-6 and IL-12p40) by mouse macrophage cell lines in a TLR2-dependent fashion, which leads to increased host innate immunity responses against the bacterium (PubMed:22174456). Lung macrophages which express low levels of TLR2 respond poorly to stimulation by M.tuberculosis LpqH (PubMed:19362712). Required for normal uptake of M.tuberculosis, a process that is inhibited by M.tuberculosis LppM (PubMed:27220037).By similarity5 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionReceptor
Biological processImmunity, Inflammatory response, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Toll-like receptor 2
Alternative name(s):
CD_antigen: CD282
Gene namesi
Name:Tlr2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1346060 Tlr2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 587ExtracellularSequence analysisAdd BLAST563
Transmembranei588 – 608HelicalSequence analysisAdd BLAST21
Topological domaini609 – 784CytoplasmicSequence analysisAdd BLAST176

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutants succumb to Staphylococcus aureus infection within 5 days.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi681P → H: Abolishes MYD88-binding and response to microbial cell wall components. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1075106
GuidetoPHARMACOLOGYi1752

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000003471225 – 784Toll-like receptor 2Add BLAST760

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi30 ↔ 36
Glycosylationi147N-linked (GlcNAc...) asparagine2 Publications1
Disulfide bondi353 ↔ 382
Glycosylationi414N-linked (GlcNAc...) asparagine2 Publications1
Disulfide bondi432 ↔ 454
Glycosylationi442N-linked (GlcNAc...) asparagine2 Publications1
Cross-linki754Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationi

Ubiquitinated at Lys-754 by PPP1R11, leading to its degradation. Deubiquitinated by USP2.1 Publication
Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ9QUN7
PaxDbiQ9QUN7
PeptideAtlasiQ9QUN7
PRIDEiQ9QUN7

PTM databases

iPTMnetiQ9QUN7
PhosphoSitePlusiQ9QUN7
SwissPalmiQ9QUN7

Expressioni

Tissue specificityi

Detected in a macrophage cell line, smooth muscle, lung, spleen, thymus, brain and adipose tissue. Cell surface expression detected in lung alveolar macrophages, dendritic macrophages and at lower levels in lung macrophages (at protein level) (PubMed:19362712).1 Publication

Gene expression databases

CleanExiMM_TLR2

Interactioni

Subunit structurei

Interacts with LY96, TLR1 and TLR6 (via extracellular domain). TLR2 seems to exist in heterodimers with either TLR1 or TLR6 before stimulation by the ligand (PubMed:19931471). The heterodimers form bigger oligomers in response to their corresponding ligands as well as further heterotypic associations with other receptors such as CD14 and/or CD36 (By similarity). Binds MYD88 (via TIR domain). Interacts with TICAM1 (By similarity). Interacts with CNPY3 (PubMed:18780723). Interacts with ATG16L1 (By similarity). Interacts with non-modified M.tuberculosis protein MPT83 (PubMed:22174456). Interacts with PPP1R11 (PubMed:27805901).By similarity4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei349Interaction with bacterial lipopeptideBy similarity1

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-61222N
IntActiQ9QUN7, 10 interactors
STRINGi10090.ENSMUSP00000029623

Structurei

Secondary structure

1784
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9QUN7
SMRiQ9QUN7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9QUN7

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati54 – 77LRR 11 PublicationAdd BLAST24
Repeati78 – 101LRR 21 PublicationAdd BLAST24
Repeati102 – 125LRR 31 PublicationAdd BLAST24
Repeati126 – 150LRR 41 PublicationAdd BLAST25
Repeati151 – 175LRR 51 PublicationAdd BLAST25
Repeati176 – 199LRR 61 PublicationAdd BLAST24
Repeati200 – 223LRR 71 PublicationAdd BLAST24
Repeati224 – 250LRR 81 PublicationAdd BLAST27
Repeati251 – 278LRR 91 PublicationAdd BLAST28
Repeati279 – 308LRR 101 PublicationAdd BLAST30
Repeati309 – 337LRR 111 PublicationAdd BLAST29
Repeati338 – 361LRR 121 PublicationAdd BLAST24
Repeati362 – 388LRR 131 PublicationAdd BLAST27
Repeati389 – 414LRR 141 PublicationAdd BLAST26
Repeati415 – 437LRR 151 PublicationAdd BLAST23
Repeati438 – 457LRR 161 PublicationAdd BLAST20
Repeati458 – 478LRR 171 PublicationAdd BLAST21
Repeati479 – 500LRR 181 PublicationAdd BLAST22
Repeati501 – 524LRR 191 PublicationAdd BLAST24
Domaini525 – 576LRRCTAdd BLAST52
Domaini639 – 784TIRPROSITE-ProRule annotationAdd BLAST146

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi761 – 778ATG16L1-binding motifAdd BLAST18

Domaini

Ester-bound lipid substrates are bound through a crevice formed between the LRR 11 and LRR 12.
The ATG16L1-binding motif mediates interaction with ATG16L1.By similarity

Sequence similaritiesi

Belongs to the Toll-like receptor family.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4641 Eukaryota
COG4886 LUCA
HOGENOMiHOG000110611
HOVERGENiHBG108574
InParanoidiQ9QUN7
KOiK10159
PhylomeDBiQ9QUN7

Family and domain databases

Gene3Di3.40.50.10140, 1 hit
3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR000483 Cys-rich_flank_reg_C
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR000157 TIR_dom
IPR027185 TLR2
IPR035897 Toll_tir_struct_dom_sf
PANTHERiPTHR24365:SF17 PTHR24365:SF17, 1 hit
PfamiView protein in Pfam
PF13516 LRR_6, 1 hit
PF13855 LRR_8, 2 hits
PF01582 TIR, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 7 hits
SM00082 LRRCT, 1 hit
SM00255 TIR, 1 hit
SUPFAMiSSF52200 SSF52200, 1 hit
PROSITEiView protein in PROSITE
PS51450 LRR, 9 hits
PS50104 TIR, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9QUN7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLRALWLFWI LVAITVLFSK RCSAQESLSC DASGVCDGRS RSFTSIPSGL
60 70 80 90 100
TAAMKSLDLS FNKITYIGHG DLRACANLQV LILKSSRINT IEGDAFYSLG
110 120 130 140 150
SLEHLDLSDN HLSSLSSSWF GPLSSLKYLN LMGNPYQTLG VTSLFPNLTN
160 170 180 190 200
LQTLRIGNVE TFSEIRRIDF AGLTSLNELE IKALSLRNYQ SQSLKSIRDI
210 220 230 240 250
HHLTLHLSES AFLLEIFADI LSSVRYLELR DTNLARFQFS PLPVDEVSSP
260 270 280 290 300
MKKLAFRGSV LTDESFNELL KLLRYILELS EVEFDDCTLN GLGDFNPSES
310 320 330 340 350
DVVSELGKVE TVTIRRLHIP QFYLFYDLST VYSLLEKVKR ITVENSKVFL
360 370 380 390 400
VPCSFSQHLK SLEFLDLSEN LMVEEYLKNS ACKGAWPSLQ TLVLSQNHLR
410 420 430 440 450
SMQKTGEILL TLKNLTSLDI SRNTFHPMPD SCQWPEKMRF LNLSSTGIRV
460 470 480 490 500
VKTCIPQTLE VLDVSNNNLD SFSLFLPRLQ ELYISRNKLK TLPDASLFPV
510 520 530 540 550
LLVMKIRENA VSTFSKDQLG SFPKLETLEA GDNHFVCSCE LLSFTMETPA
560 570 580 590 600
LAQILVDWPD SYLCDSPPRL HGHRLQDARP SVLECHQAAL VSGVCCALLL
610 620 630 640 650
LILLVGALCH HFHGLWYLRM MWAWLQAKRK PKKAPCRDVC YDAFVSYSEQ
660 670 680 690 700
DSHWVENLMV QQLENSDPPF KLCLHKRDFV PGKWIIDNII DSIEKSHKTV
710 720 730 740 750
FVLSENFVRS EWCKYELDFS HFRLFDENND AAILVLLEPI ERKAIPQRFC
760 770 780
KLRKIMNTKT YLEWPLDEGQ QEVFWVNLRT AIKS
Length:784
Mass (Da):89,449
Last modified:May 1, 2000 - v1
Checksum:i606D56BF85F320A2
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3X8Y8G3X8Y8_MOUSE
Toll-like receptor 2
Tlr2 mCG_17071
784Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti59L → P in BAB23770 (PubMed:16141072).Curated1
Sequence conflicti82I → M in BAB23770 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF185284 mRNA Translation: AAF04277.1
AF124741 mRNA Translation: AAD46481.1
AF216289 mRNA Translation: AAF28345.1
AF165189 mRNA Translation: AAD49335.1
AK005043 mRNA Translation: BAB23770.1
AK154504 mRNA Translation: BAE32635.1
CCDSiCCDS17435.1
RefSeqiNP_036035.3, NM_011905.3
UniGeneiMm.87596

Genome annotation databases

GeneIDi24088
KEGGimmu:24088

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF185284 mRNA Translation: AAF04277.1
AF124741 mRNA Translation: AAD46481.1
AF216289 mRNA Translation: AAF28345.1
AF165189 mRNA Translation: AAD49335.1
AK005043 mRNA Translation: BAB23770.1
AK154504 mRNA Translation: BAE32635.1
CCDSiCCDS17435.1
RefSeqiNP_036035.3, NM_011905.3
UniGeneiMm.87596

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Z81X-ray1.80A27-506[»]
2Z82X-ray2.60A27-506[»]
3A79X-ray2.90A1-506[»]
3A7BX-ray2.53A1-506[»]
3A7CX-ray2.40A1-506[»]
5D3IX-ray3.20A25-589[»]
ProteinModelPortaliQ9QUN7
SMRiQ9QUN7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61222N
IntActiQ9QUN7, 10 interactors
STRINGi10090.ENSMUSP00000029623

Chemistry databases

ChEMBLiCHEMBL1075106
GuidetoPHARMACOLOGYi1752

PTM databases

iPTMnetiQ9QUN7
PhosphoSitePlusiQ9QUN7
SwissPalmiQ9QUN7

Proteomic databases

MaxQBiQ9QUN7
PaxDbiQ9QUN7
PeptideAtlasiQ9QUN7
PRIDEiQ9QUN7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24088
KEGGimmu:24088

Organism-specific databases

CTDi7097
MGIiMGI:1346060 Tlr2

Phylogenomic databases

eggNOGiKOG4641 Eukaryota
COG4886 LUCA
HOGENOMiHOG000110611
HOVERGENiHBG108574
InParanoidiQ9QUN7
KOiK10159
PhylomeDBiQ9QUN7

Miscellaneous databases

EvolutionaryTraceiQ9QUN7
PROiPR:Q9QUN7
SOURCEiSearch...

Gene expression databases

CleanExiMM_TLR2

Family and domain databases

Gene3Di3.40.50.10140, 1 hit
3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR000483 Cys-rich_flank_reg_C
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR000157 TIR_dom
IPR027185 TLR2
IPR035897 Toll_tir_struct_dom_sf
PANTHERiPTHR24365:SF17 PTHR24365:SF17, 1 hit
PfamiView protein in Pfam
PF13516 LRR_6, 1 hit
PF13855 LRR_8, 2 hits
PF01582 TIR, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 7 hits
SM00082 LRRCT, 1 hit
SM00255 TIR, 1 hit
SUPFAMiSSF52200 SSF52200, 1 hit
PROSITEiView protein in PROSITE
PS51450 LRR, 9 hits
PS50104 TIR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTLR2_MOUSE
AccessioniPrimary (citable) accession number: Q9QUN7
Secondary accession number(s): Q3U400, Q9DBC4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again