UniProtKB - Q9QUN3 (BLNK_MOUSE)
Protein
B-cell linker protein
Gene
Blnk
Organism
Mus musculus (Mouse)
Status
Functioni
Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.3 Publications
GO - Molecular functioni
- enzyme binding Source: MGI
- protein tyrosine kinase binding Source: MGI
- SH2 domain binding Source: MGI
- signaling adaptor activity Source: MGI
GO - Biological processi
- B cell activation Source: UniProtKB-KW
- B cell receptor signaling pathway Source: MGI
- intracellular signal transduction Source: MGI
- positive regulation of gene expression Source: ARUK-UCL
- transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Keywordsi
Biological process | B-cell activation |
Enzyme and pathway databases
Reactomei | R-MMU-983695, Antigen activates B Cell Receptor (BCR) leading to generation of second messengers |
Names & Taxonomyi
Protein namesi | Recommended name: B-cell linker proteinAlternative name(s): B-cell adapter containing a SH2 domain protein B-cell adapter containing a Src homology 2 domain protein Cytoplasmic adapter protein Lymphocyte antigen 57 Src homology 2 domain-containing leukocyte protein of 65 kDa Short name: Slp-65 |
Gene namesi | Name:Blnk Synonyms:Bash, Ly57, Slp65 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96878, Blnk |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Other locations
- Cytoplasm By similarity
Note: BCR activation results in the translocation to membrane fraction.By similarity
Cytosol
- cytosol Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cytoplasm Source: MGI
- cytoplasmic ribonucleoprotein granule Source: MGI
- membrane Source: MGI
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 52 | Y → F: No effect on pre-BCR down-regulation. 1 Publication | 1 | |
Mutagenesisi | 96 | Y → F: Fails to induce pre-BCR down-regulation, leading to splenomegaly and leukemia. 1 Publication | 1 | |
Mutagenesisi | 373 | R → L: Abolishes binding to CD79A and SYK. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064941 | 1 – 457 | B-cell linker proteinAdd BLAST | 457 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 72 | Phosphotyrosine; by SYKBy similarity | 1 | |
Modified residuei | 84 | Phosphotyrosine; by SYKBy similarity | 1 | |
Modified residuei | 96 | Phosphotyrosine; by SYKBy similarity | 1 | |
Modified residuei | 178 | Phosphotyrosine; by SYKBy similarity | 1 | |
Modified residuei | 189 | Phosphotyrosine; by SYKBy similarity | 1 |
Post-translational modificationi
Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca2+ and MAPK signaling pathways (By similarity). Phosphorylation of Tyr-96 is required for the binding of BTK.By similarity1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q9QUN3 |
PaxDbi | Q9QUN3 |
PeptideAtlasi | Q9QUN3 |
PRIDEi | Q9QUN3 |
PTM databases
iPTMneti | Q9QUN3 |
PhosphoSitePlusi | Q9QUN3 |
Expressioni
Tissue specificityi
Expressed in the spleen and weakly in thymus, no expression was seen in liver, testis, or brain. Expressed in B-cell lines representing different developmental stages from the pre-B to the plasma cell stage, but not in a T-cell or a fibroblast cell line.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000061132, Expressed in bone marrow and 173 other tissues |
ExpressionAtlasi | Q9QUN3, baseline and differential |
Genevisiblei | Q9QUN3, MM |
Interactioni
Subunit structurei
Associates with PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion.
Interacts with VAV3, PLCG2 and GRB2 (By similarity).
Interacts through its SH2 domain with CD79A.
Interacts (via SH2 domain) with SYK; phosphorylated and activated by SYK.
Interacts (via SH2 domain) with SCIMP; this interaction is dependent on phosphorylation of SCIMP 'Tyr-120' (PubMed:21930792, PubMed:28290451).
By similarity7 PublicationsGO - Molecular functioni
- enzyme binding Source: MGI
- protein tyrosine kinase binding Source: MGI
- SH2 domain binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201236, 5 interactors |
CORUMi | Q9QUN3 |
IntActi | Q9QUN3, 7 interactors |
MINTi | Q9QUN3 |
STRINGi | 10090.ENSMUSP00000057844 |
Miscellaneous databases
RNActi | Q9QUN3, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9QUN3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9QUN3 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 347 – 454 | SH2PROSITE-ProRule annotationAdd BLAST | 108 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 130 – 330 | Pro-richAdd BLAST | 201 |
Keywords - Domaini
SH2 domainPhylogenomic databases
eggNOGi | ENOG502QUXR, Eukaryota |
GeneTreei | ENSGT00940000155715 |
HOGENOMi | CLU_043673_0_0_1 |
InParanoidi | Q9QUN3 |
OMAi | VHQKPIP |
OrthoDBi | 556782at2759 |
PhylomeDBi | Q9QUN3 |
TreeFami | TF326567 |
Family and domain databases
Gene3Di | 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR000980, SH2 IPR036860, SH2_dom_sf |
Pfami | View protein in Pfam PF00017, SH2, 1 hit |
SMARTi | View protein in SMART SM00252, SH2, 1 hit |
SUPFAMi | SSF55550, SSF55550, 1 hit |
PROSITEi | View protein in PROSITE PS50001, SH2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9QUN3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMDKIKKLK VKGPPSVPRR
60 70 80 90 100
DYALDSPADE EEQWSDDFDS DYENPDEHSD SEMYVMPAEE TGDDSYEPPP
110 120 130 140 150
AEQQTRVVHP ALPFTRGEYV DNRSSQRHSP PFSKTLPSKP SWPSAKARLA
160 170 180 190 200
STLPAPNSLQ KPQVPPKPKD LLEDEADYVV PVEDNDENYI HPRESSPPPA
210 220 230 240 250
EKAPMVNRST KPNSSSKHMS PPGTVAGRNS GVWDSKSSLP AAPSPLPRAG
260 270 280 290 300
KKPATPLKTT PVPPLPNASN VCEEKPVPAE RHRGSSHRQD TVQSPVFPPT
310 320 330 340 350
QKPVHQKPVP LPRFPEAGSP AADGPFHSFP FNSTFADQEA ELLGKPWYAG
360 370 380 390 400
ACDRKSAEEA LHRSNKDGSF LIRKSSGHDS KQPYTLVAFF NKRVYNIPVR
410 420 430 440 450
FIEATKQYAL GKKKNGEEYF GSVVEIVNSH QHNPLVLIDS QNNTKDSTRL
KYAVKVS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD3YWR2 | D3YWR2_MOUSE | B-cell linker protein | Blnk | 454 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 333 | S → L in AAC40206 (PubMed:9697839).Curated | 1 | |
Sequence conflicti | 340 | A → G in AAC40206 (PubMed:9697839).Curated | 1 | |
Sequence conflicti | 356 | S → F in AAC40206 (PubMed:9697839).Curated | 1 | |
Sequence conflicti | 376 | S → F in AAC40206 (PubMed:9697839).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF068182 mRNA Translation: AAC40206.1 Y17159 mRNA Translation: CAA76666.1 AB015290 mRNA Translation: BAA34944.1 AJ298054 Genomic DNA Translation: CAC18565.1 BC059785 mRNA Translation: AAH59785.1 |
CCDSi | CCDS37983.1 |
RefSeqi | NP_032554.2, NM_008528.4 |
Genome annotation databases
Ensembli | ENSMUST00000054769; ENSMUSP00000057844; ENSMUSG00000061132 |
GeneIDi | 17060 |
KEGGi | mmu:17060 |
UCSCi | uc008hll.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF068182 mRNA Translation: AAC40206.1 Y17159 mRNA Translation: CAA76666.1 AB015290 mRNA Translation: BAA34944.1 AJ298054 Genomic DNA Translation: CAC18565.1 BC059785 mRNA Translation: AAH59785.1 |
CCDSi | CCDS37983.1 |
RefSeqi | NP_032554.2, NM_008528.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2EO6 | NMR | - | A | 330-457 | [»] | |
SMRi | Q9QUN3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 201236, 5 interactors |
CORUMi | Q9QUN3 |
IntActi | Q9QUN3, 7 interactors |
MINTi | Q9QUN3 |
STRINGi | 10090.ENSMUSP00000057844 |
PTM databases
iPTMneti | Q9QUN3 |
PhosphoSitePlusi | Q9QUN3 |
Proteomic databases
MaxQBi | Q9QUN3 |
PaxDbi | Q9QUN3 |
PeptideAtlasi | Q9QUN3 |
PRIDEi | Q9QUN3 |
Protocols and materials databases
Antibodypediai | 3990, 707 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000054769; ENSMUSP00000057844; ENSMUSG00000061132 |
GeneIDi | 17060 |
KEGGi | mmu:17060 |
UCSCi | uc008hll.1, mouse |
Organism-specific databases
CTDi | 29760 |
MGIi | MGI:96878, Blnk |
Phylogenomic databases
eggNOGi | ENOG502QUXR, Eukaryota |
GeneTreei | ENSGT00940000155715 |
HOGENOMi | CLU_043673_0_0_1 |
InParanoidi | Q9QUN3 |
OMAi | VHQKPIP |
OrthoDBi | 556782at2759 |
PhylomeDBi | Q9QUN3 |
TreeFami | TF326567 |
Enzyme and pathway databases
Reactomei | R-MMU-983695, Antigen activates B Cell Receptor (BCR) leading to generation of second messengers |
Miscellaneous databases
BioGRID-ORCSi | 17060, 1 hit in 17 CRISPR screens |
ChiTaRSi | Blnk, mouse |
EvolutionaryTracei | Q9QUN3 |
PROi | PR:Q9QUN3 |
RNActi | Q9QUN3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000061132, Expressed in bone marrow and 173 other tissues |
ExpressionAtlasi | Q9QUN3, baseline and differential |
Genevisiblei | Q9QUN3, MM |
Family and domain databases
Gene3Di | 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR000980, SH2 IPR036860, SH2_dom_sf |
Pfami | View protein in Pfam PF00017, SH2, 1 hit |
SMARTi | View protein in SMART SM00252, SH2, 1 hit |
SUPFAMi | SSF55550, SSF55550, 1 hit |
PROSITEi | View protein in PROSITE PS50001, SH2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BLNK_MOUSE | |
Accessioni | Q9QUN3Primary (citable) accession number: Q9QUN3 Secondary accession number(s): O88504 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references