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UniProtKB - Q9QUN3 (BLNK_MOUSE)

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Protein

B-cell linker protein

Gene

Blnk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.3 Publications

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Biological processB-cell activation

Enzyme and pathway databases

ReactomeiR-MMU-983695 Antigen activates B Cell Receptor (BCR) leading to generation of second messengers

Names & Taxonomyi

Protein namesi
Recommended name:
B-cell linker protein
Alternative name(s):
B-cell adapter containing a SH2 domain protein
B-cell adapter containing a Src homology 2 domain protein
Cytoplasmic adapter protein
Lymphocyte antigen 57
Src homology 2 domain-containing leukocyte protein of 65 kDa
Short name:
Slp-65
Gene namesi
Name:Blnk
Synonyms:Bash, Ly57, Slp65
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:96878 Blnk

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi52Y → F: No effect on pre-BCR down-regulation. 1 Publication1
Mutagenesisi96Y → F: Fails to induce pre-BCR down-regulation, leading to splenomegaly and leukemia. 1 Publication1
Mutagenesisi373R → L: Abolishes binding to CD79A and SYK. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000649411 – 457B-cell linker proteinAdd BLAST457

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei72Phosphotyrosine; by SYKBy similarity1
Modified residuei84Phosphotyrosine; by SYKBy similarity1
Modified residuei96Phosphotyrosine; by SYKBy similarity1
Modified residuei178Phosphotyrosine; by SYKBy similarity1
Modified residuei189Phosphotyrosine; by SYKBy similarity1

Post-translational modificationi

Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca2+ and MAPK signaling pathways (By similarity). Phosphorylation of Tyr-96 is required for the binding of BTK.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9QUN3
PaxDbiQ9QUN3
PeptideAtlasiQ9QUN3
PRIDEiQ9QUN3

PTM databases

iPTMnetiQ9QUN3
PhosphoSitePlusiQ9QUN3

Expressioni

Tissue specificityi

Expressed in the spleen and weakly in thymus, no expression was seen in liver, testis, or brain. Expressed in B-cell lines representing different developmental stages from the pre-B to the plasma cell stage, but not in a T-cell or a fibroblast cell line.1 Publication

Gene expression databases

BgeeiENSMUSG00000061132
CleanExiMM_BLNK
ExpressionAtlasiQ9QUN3 baseline and differential
GenevisibleiQ9QUN3 MM

Interactioni

Subunit structurei

Associates with PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with VAV3, PLCG2 and GRB2 (By similarity). Interacts through its SH2 domain with CD79A. Interacts (via SH2 domain) with SYK; phosphorylated and activated by SYK. Interacts with SCIMP.By similarity6 Publications

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi201236, 5 interactors
CORUMiQ9QUN3
IntActiQ9QUN3, 6 interactors
MINTiQ9QUN3
STRINGi10090.ENSMUSP00000057844

Structurei

Secondary structure

1457
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi334 – 340Combined sources7
Turni341 – 344Combined sources4
Beta strandi346 – 351Combined sources6
Helixi354 – 364Combined sources11
Beta strandi372 – 374Combined sources3
Beta strandi383 – 390Combined sources8
Beta strandi393 – 399Combined sources7
Turni403 – 406Combined sources4
Beta strandi410 – 412Combined sources3
Beta strandi420 – 422Combined sources3
Helixi423 – 432Combined sources10
Beta strandi440 – 442Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EO6NMR-A330-457[»]
ProteinModelPortaliQ9QUN3
SMRiQ9QUN3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9QUN3

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini347 – 454SH2PROSITE-ProRule annotationAdd BLAST108

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi130 – 330Pro-richAdd BLAST201

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiENOG410IGWN Eukaryota
ENOG410XYB6 LUCA
GeneTreeiENSGT00530000063094
HOGENOMiHOG000088646
HOVERGENiHBG053147
InParanoidiQ9QUN3
KOiK07371
OMAiDSEMYVL
OrthoDBiEOG091G053I
PhylomeDBiQ9QUN3
TreeFamiTF326567

Family and domain databases

Gene3Di3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR000980 SH2
IPR036860 SH2_dom_sf
PfamiView protein in Pfam
PF00017 SH2, 1 hit
SMARTiView protein in SMART
SM00252 SH2, 1 hit
SUPFAMiSSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50001 SH2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9QUN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMDKIKKLK VKGPPSVPRR 
        60         70         80         90        100
DYALDSPADE EEQWSDDFDS DYENPDEHSD SEMYVMPAEE TGDDSYEPPP 
       110        120        130        140        150
AEQQTRVVHP ALPFTRGEYV DNRSSQRHSP PFSKTLPSKP SWPSAKARLA 
       160        170        180        190        200
STLPAPNSLQ KPQVPPKPKD LLEDEADYVV PVEDNDENYI HPRESSPPPA 
       210        220        230        240        250
EKAPMVNRST KPNSSSKHMS PPGTVAGRNS GVWDSKSSLP AAPSPLPRAG 
       260        270        280        290        300
KKPATPLKTT PVPPLPNASN VCEEKPVPAE RHRGSSHRQD TVQSPVFPPT 
       310        320        330        340        350
QKPVHQKPVP LPRFPEAGSP AADGPFHSFP FNSTFADQEA ELLGKPWYAG 
       360        370        380        390        400
ACDRKSAEEA LHRSNKDGSF LIRKSSGHDS KQPYTLVAFF NKRVYNIPVR 
       410        420        430        440        450
FIEATKQYAL GKKKNGEEYF GSVVEIVNSH QHNPLVLIDS QNNTKDSTRL 

KYAVKVS
Length:457
Mass (Da):50,671
Last modified:May 1, 2000 - v1
Checksum:i66C93D4FDDF9D260
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti333S → L in AAC40206 (PubMed:9697839).Curated1
Sequence conflicti340A → G in AAC40206 (PubMed:9697839).Curated1
Sequence conflicti356S → F in AAC40206 (PubMed:9697839).Curated1
Sequence conflicti376S → F in AAC40206 (PubMed:9697839).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF068182 mRNA Translation: AAC40206.1
Y17159 mRNA Translation: CAA76666.1
AB015290 mRNA Translation: BAA34944.1
AJ298054 Genomic DNA Translation: CAC18565.1
BC059785 mRNA Translation: AAH59785.1
CCDSiCCDS37983.1
RefSeqiNP_032554.2, NM_008528.4
UniGeneiMm.9749

Genome annotation databases

EnsembliENSMUST00000054769; ENSMUSP00000057844; ENSMUSG00000061132
GeneIDi17060
KEGGimmu:17060
UCSCiuc008hll.1 mouse

Similar proteinsi

Entry informationi

Entry nameiBLNK_MOUSE
AccessioniPrimary (citable) accession number: Q9QUN3
Secondary accession number(s): O88504
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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