UniProtKB - Q9QUM4 (SLAF1_MOUSE)
Signaling lymphocytic activation molecule
Slamf1
Functioni
GO - Molecular functioni
- identical protein binding Source: MGI
- SH2 domain binding Source: MGI
- signaling receptor activity Source: MGI
GO - Biological processi
- cell adhesion Source: UniProtKB-KW
- innate immune response Source: UniProtKB-KW
- leukocyte chemotaxis involved in inflammatory response Source: MGI
- myeloid dendritic cell activation involved in immune response Source: MGI
- natural killer cell differentiation Source: UniProtKB
- natural killer cell proliferation Source: UniProtKB
- negative regulation of CD40 signaling pathway Source: MGI
- negative regulation of interferon-gamma production Source: UniProtKB
- negative regulation of interleukin-12 production Source: MGI
- negative regulation of interleukin-6 production Source: MGI
- negative regulation of T cell cytokine production Source: UniProtKB
- negative regulation of tumor necrosis factor production Source: MGI
- phagocytosis Source: UniProtKB-KW
- positive regulation of activated T cell proliferation Source: UniProtKB
- positive regulation of dendritic cell chemotaxis Source: MGI
- positive regulation of ERK1 and ERK2 cascade Source: MGI
- positive regulation of interferon-gamma production Source: UniProtKB
- positive regulation of JNK cascade Source: MGI
- positive regulation of macrophage chemotaxis Source: MGI
- regulation of catalytic activity Source: MGI
- regulation of vesicle fusion Source: MGI
- T-helper 1 cell cytokine production Source: UniProtKB
Keywordsi
Molecular function | Receptor |
Biological process | Adaptive immunity, Cell adhesion, Immunity, Innate immunity, Phagocytosis |
Names & Taxonomyi
Protein namesi | Recommended name: Signaling lymphocytic activation moleculeAlternative name(s): SLAM family member 1 CD_antigen: CD150 |
Gene namesi | Name:Slamf1 Synonyms:Slam |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1351314, Slamf1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein
Note: Present on the surface of B-cells and T-cells. Located at the plasma membrane contacts between neighboring T cells.By similarity
Plasma Membrane
- external side of plasma membrane Source: MGI
Other locations
- cell surface Source: MGI
- integral component of membrane Source: UniProtKB-KW
- phagocytic vesicle Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 25 – 242 | ExtracellularSequence analysisAdd BLAST | 218 | |
Transmembranei | 243 – 265 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 266 – 343 | CytoplasmicSequence analysisAdd BLAST | 78 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 288 | Y → A: Greatly reduces SLAMF1:SH2D1A-mediated intracellular tyrosine phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 315 | Y → A: Abolishes SLAMF1:SH2D1A-mediated intracellular tyrosine phosphorylation, no effect on interaction with SH2D1A; when associated with A-335. 1 Publication | 1 | |
Mutagenesisi | 335 | Y → A: Abolishes SLAMF1:SH2D1A-mediated intracellular tyrosine phosphorylation, no effect on interaction with SH2D1A; when associated with A-315. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | Sequence analysisAdd BLAST | 24 | |
ChainiPRO_0000014960 | 25 – 343 | Signaling lymphocytic activation moleculeAdd BLAST | 319 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 54 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 58 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 103 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 126 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 151 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 158 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 161 ↔ 232 | PROSITE-ProRule annotation | ||
Disulfide bondi | 167 ↔ 212 | PROSITE-ProRule annotation | ||
Glycosylationi | 192 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 211 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 226 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 288 | Phosphotyrosine; by FYNBy similarity | 1 | |
Modified residuei | 315 | Phosphotyrosine; by FYNBy similarity | 1 | |
Modified residuei | 335 | Phosphotyrosine; by FYNBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | Q9QUM4 |
PaxDbi | Q9QUM4 |
PRIDEi | Q9QUM4 |
PTM databases
GlyGeni | Q9QUM4, 9 sites |
iPTMneti | Q9QUM4 |
PhosphoSitePlusi | Q9QUM4 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000015316, Expressed in blood and 69 other tissues |
Genevisiblei | Q9QUM4, MM |
Interactioni
Subunit structurei
Interacts (via cytoplasmic domain) with SH2D1A and SH2D1B; SH2D1A mediates association with FYN; SH2D1A binds to phosphorylated and not phosphorylated ITSM 1 (PubMed:9774102, PubMed:16847311, PubMed:11477403.)
Interacts (via cytoplasmic domain phosphorylated on tyrosine residues) with INPP5D and PTPN11; presence of SH2D1A facilitates binding to INPP5D (By similarity).
Interacts with MAP4K1 (By similarity).
Interacts with PIK3C3, BECN1 and UVRAG; indicative for an association with PI3K complex II (PI3KC3-C2) (PubMed:22493499).
By similarity6 PublicationsBinary interactionsi
Hide detailsQ9QUM4
GO - Molecular functioni
- identical protein binding Source: MGI
- SH2 domain binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 205139, 2 interactors |
CORUMi | Q9QUM4 |
IntActi | Q9QUM4, 11 interactors |
MINTi | Q9QUM4 |
STRINGi | 10090.ENSMUSP00000015460 |
Miscellaneous databases
RNActi | Q9QUM4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 29 – 138 | Ig-like V-typeAdd BLAST | 110 | |
Domaini | 145 – 228 | Ig-like C2-typeAdd BLAST | 84 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 286 – 291 | ITSM 1By similarity | 6 | |
Motifi | 313 – 318 | SH2-bindingSequence analysis | 6 | |
Motifi | 333 – 338 | ITSM 2By similarity | 6 |
Domaini
Keywords - Domaini
Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502SVMG, Eukaryota |
GeneTreei | ENSGT00990000203668 |
HOGENOMi | CLU_066453_0_0_1 |
InParanoidi | Q9QUM4 |
OMAi | QWGLYAG |
PhylomeDBi | Q9QUM4 |
TreeFami | TF334964 |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR010407, Sig_lymph_act_molc_N |
Pfami | View protein in Pfam PF06214, SLAM, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDPKGSLSWR ILLFLSLAFE LSYGTGGGVM DCPVILQKLG QDTWLPLTNE
60 70 80 90 100
HQINKSVNKS VRILVTMATS PGSKSNKKIV SFDLSKGSYP DHLEDGYHFQ
110 120 130 140 150
SKNLSLKILG NRRESEGWYL VSVEENVSVQ QFCKQLKLYE QVSPPEIKVL
160 170 180 190 200
NKTQENENGT CSLLLACTVK KGDHVTYSWS DEAGTHLLSR ANRSHLLHIT
210 220 230 240 250
LSNQHQDSIY NCTASNPVSS ISRTFNLSSQ ACKQESSSES SPWMQYTLVP
260 270 280 290 300
LGVVIIFILV FTAIIMMKRQ GKSNHCQPPV EEKSLTIYAQ VQKSGPQEKK
310 320 330 340
LHDALTDQDP CTTIYVAATE PAPESVQEPN PTTVYASVTL PES
The sequence of this isoform differs from the canonical sequence as follows:
296-343: PQEKKLHDAL...VYASVTLPES → VRSMPHLAGVSVIFRTGFLIAALHTTMVLQGLLE
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002570 | 296 – 343 | PQEKK…TLPES → VRSMPHLAGVSVIFRTGFLI AALHTTMVLQGLLE in isoform Short. 1 PublicationAdd BLAST | 48 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF149791 mRNA Translation: AAF22231.1 AF149792 mRNA Translation: AAF22232.1 AF164523 , AF164519, AF164520, AF164521, AF164522 Genomic DNA Translation: AAF13818.1 AF160990 mRNA Translation: AAF14535.1 |
CCDSi | CCDS15502.1 [Q9QUM4-1] |
RefSeqi | NP_038758.2, NM_013730.4 [Q9QUM4-1] |
Genome annotation databases
Ensembli | ENSMUST00000015460; ENSMUSP00000015460; ENSMUSG00000015316 [Q9QUM4-1] |
GeneIDi | 27218 |
KEGGi | mmu:27218 |
UCSCi | uc007dpc.1, mouse [Q9QUM4-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF149791 mRNA Translation: AAF22231.1 AF149792 mRNA Translation: AAF22232.1 AF164523 , AF164519, AF164520, AF164521, AF164522 Genomic DNA Translation: AAF13818.1 AF160990 mRNA Translation: AAF14535.1 |
CCDSi | CCDS15502.1 [Q9QUM4-1] |
RefSeqi | NP_038758.2, NM_013730.4 [Q9QUM4-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 205139, 2 interactors |
CORUMi | Q9QUM4 |
IntActi | Q9QUM4, 11 interactors |
MINTi | Q9QUM4 |
STRINGi | 10090.ENSMUSP00000015460 |
PTM databases
GlyGeni | Q9QUM4, 9 sites |
iPTMneti | Q9QUM4 |
PhosphoSitePlusi | Q9QUM4 |
Proteomic databases
jPOSTi | Q9QUM4 |
PaxDbi | Q9QUM4 |
PRIDEi | Q9QUM4 |
Protocols and materials databases
Antibodypediai | 3725, 969 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000015460; ENSMUSP00000015460; ENSMUSG00000015316 [Q9QUM4-1] |
GeneIDi | 27218 |
KEGGi | mmu:27218 |
UCSCi | uc007dpc.1, mouse [Q9QUM4-1] |
Organism-specific databases
CTDi | 6504 |
MGIi | MGI:1351314, Slamf1 |
Phylogenomic databases
eggNOGi | ENOG502SVMG, Eukaryota |
GeneTreei | ENSGT00990000203668 |
HOGENOMi | CLU_066453_0_0_1 |
InParanoidi | Q9QUM4 |
OMAi | QWGLYAG |
PhylomeDBi | Q9QUM4 |
TreeFami | TF334964 |
Miscellaneous databases
BioGRID-ORCSi | 27218, 0 hits in 17 CRISPR screens |
PROi | PR:Q9QUM4 |
RNActi | Q9QUM4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000015316, Expressed in blood and 69 other tissues |
Genevisiblei | Q9QUM4, MM |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR010407, Sig_lymph_act_molc_N |
Pfami | View protein in Pfam PF06214, SLAM, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SLAF1_MOUSE | |
Accessioni | Q9QUM4Primary (citable) accession number: Q9QUM4 Secondary accession number(s): Q9QXZ3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot