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Protein

Transforming protein RhoA

Gene

Rhoa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). Required for the apical junction formation of keratinocyte cell-cell adhesion. Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis (PubMed:9635436).By similarity3 Publications

Activity regulationi

GTP hydrolysis is stimulated by ARHGAP30.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 19GTPBy similarity8
Nucleotide bindingi59 – 63GTPBy similarity5
Nucleotide bindingi117 – 120GTPBy similarity4

GO - Molecular functioni

  • GDP binding Source: MGI
  • GTPase activity Source: MGI
  • GTP binding Source: UniProtKB
  • myosin binding Source: MGI
  • protein domain specific binding Source: MGI
  • protein kinase binding Source: GO_Central
  • Rho GDP-dissociation inhibitor binding Source: MGI

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-114604 GPVI-mediated activation cascade
R-MMU-193634 Axonal growth inhibition (RHOA activation)
R-MMU-194840 Rho GTPase cycle
R-MMU-198203 PI3K/AKT activation
R-MMU-209563 Axonal growth stimulation
R-MMU-2173791 TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition)
R-MMU-392451 G beta:gamma signalling through PI3Kgamma
R-MMU-3928662 EPHB-mediated forward signaling
R-MMU-3928663 EPHA-mediated growth cone collapse
R-MMU-4086400 PCP/CE pathway
R-MMU-416482 G alpha (12/13) signalling events
R-MMU-416550 Sema4D mediated inhibition of cell attachment and migration
R-MMU-416572 Sema4D induced cell migration and growth-cone collapse
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-5625740 RHO GTPases activate PKNs
R-MMU-5625900 RHO GTPases activate CIT
R-MMU-5625970 RHO GTPases activate KTN1
R-MMU-5627117 RHO GTPases Activate ROCKs
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-5666185 RHO GTPases Activate Rhotekin and Rhophilins
R-MMU-5689896 Ovarian tumor domain proteases
R-MMU-6785631 ERBB2 Regulates Cell Motility
R-MMU-6798695 Neutrophil degranulation
R-MMU-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases
R-MMU-8985586 SLIT2:ROBO1 increases RHOA activity

Names & Taxonomyi

Protein namesi
Recommended name:
Transforming protein RhoA
Gene namesi
Name:Rhoa
Synonyms:Arha, Arha2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1096342 Rhoa

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000304131 – 190Transforming protein RhoAAdd BLAST190
PropeptideiPRO_0000030414191 – 193Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188Phosphoserine; by PKG/PRKG1By similarity1
Modified residuei190Cysteine methyl esterBy similarity1
Lipidationi190S-geranylgeranyl cysteineBy similarity1

Post-translational modificationi

Ubiquitinated by the BCR(KCTD13) and BCR(TNFAIP1) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and synaptic transmission in neurons.1 Publication
Phosphorylation by PRKG1 at Ser-188 inactivates RHOA signaling (By similarity). Phosphorylation by SLK at Ser-188 in response to AGTR2 activation (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation, Ubl conjugation

Proteomic databases

EPDiQ9QUI0
MaxQBiQ9QUI0
PaxDbiQ9QUI0
PRIDEiQ9QUI0

PTM databases

iPTMnetiQ9QUI0
PhosphoSitePlusiQ9QUI0
SwissPalmiQ9QUI0

Expressioni

Inductioni

Up-regulated during keratinocyte differentiation.1 Publication

Gene expression databases

BgeeiENSMUSG00000007815 Expressed in 301 organ(s), highest expression level in seminal vesicle
CleanExiMM_RHOA
ExpressionAtlasiQ9QUI0 baseline and differential
GenevisibleiQ9QUI0 MM

Interactioni

Subunit structurei

Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF2 and ARHGEF3. Interacts with PLCE1 and AKAP13. Interacts with DIAPH1. Interacts (in the constitutively activated, GTP-bound form) with DGKQ. Interacts with RACK1; enhances RHOA activation. Interacts with PKP4; the interaction is detected at the midbody. Interacts (GTP-bound form preferentially) with PKN2; the interaction stimulates autophosphorylation and phosphorylation of PKN2 (By similarity). Interacts with NET1, ARHGEF28 and RTKN. Interacts with ARHGDIA; this interaction inactivates and stabilizes RHOA. Interacts with ARHGDIB (By similarity). Interacts (GTP-bound form) with KCNA2 (via cytoplasmic N-terminal domain) (PubMed:9635436). Interacts (via GTP-bound form) with RIPOR1 (via N-terminus); this interaction links RHOA to STK24 and STK26 kinases (By similarity). Interacts with RIPOR2 (via active GTP- or inactive GDP-bound forms); this interaction is direct, blocks the loading of GTP to RHOA and decreases upon chemokine CCL19 stimulation in primary T lymphocytes (By similarity).By similarity1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198192, 37 interactors
DIPiDIP-29984N
IntActiQ9QUI0, 36 interactors
MINTiQ9QUI0
STRINGi10090.ENSMUSP00000007959

Structurei

Secondary structure

1193
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9QUI0
SMRiQ9QUI0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi34 – 42Effector regionSequence analysis9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi182 – 187Arg/Lys-rich (basic)6

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000119020
HOGENOMiHOG000233974
HOVERGENiHBG009351
InParanoidiQ9QUI0
KOiK04513
OMAiQKIGARH
OrthoDBiEOG091G0QVS
PhylomeDBiQ9QUI0
TreeFamiTF300837

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q9QUI0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYVADIEVDG
60 70 80 90 100
KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT
110 120 130 140 150
PEVKHFCPNV PIILVGNKKD LRNDEHTRRE LAKMKQEPVK PEEGRDMANR
160 170 180 190
IGAFGYMECS AKTKDGVREV FEMATRAALQ ARRGKKKSGC LIL
Length:193
Mass (Da):21,782
Last modified:May 1, 2000 - v1
Checksum:iC4C8BDC31FF858BC
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A6YXF6A0A0A6YXF6_MOUSE
Transforming protein RhoA
Rhoa
165Annotation score:
A0A0A6YWJ1A0A0A6YWJ1_MOUSE
Transforming protein RhoA
Rhoa
67Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68R → C in AAC23710 (PubMed:9598304).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014371 mRNA Translation: AAC23710.1
AF178958 mRNA Translation: AAD52675.1
AF178959 mRNA Translation: AAD52676.1
AF178960 mRNA Translation: AAD52677.1
AF178961 mRNA Translation: AAD52678.1
AK077606 mRNA Translation: BAC36896.1
AK083624 mRNA Translation: BAC38971.1
BC068115 mRNA Translation: AAH68115.1
CCDSiCCDS23521.1
RefSeqiNP_001300890.1, NM_001313961.1
NP_001300891.1, NM_001313962.1
NP_058082.2, NM_016802.5
UniGeneiMm.318359
Mm.757

Genome annotation databases

EnsembliENSMUST00000007959; ENSMUSP00000007959; ENSMUSG00000007815
GeneIDi11848
KEGGimmu:11848
UCSCiuc009rpe.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014371 mRNA Translation: AAC23710.1
AF178958 mRNA Translation: AAD52675.1
AF178959 mRNA Translation: AAD52676.1
AF178960 mRNA Translation: AAD52677.1
AF178961 mRNA Translation: AAD52678.1
AK077606 mRNA Translation: BAC36896.1
AK083624 mRNA Translation: BAC38971.1
BC068115 mRNA Translation: AAH68115.1
CCDSiCCDS23521.1
RefSeqiNP_001300890.1, NM_001313961.1
NP_001300891.1, NM_001313962.1
NP_058082.2, NM_016802.5
UniGeneiMm.318359
Mm.757

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4F38X-ray2.80A1-191[»]
ProteinModelPortaliQ9QUI0
SMRiQ9QUI0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198192, 37 interactors
DIPiDIP-29984N
IntActiQ9QUI0, 36 interactors
MINTiQ9QUI0
STRINGi10090.ENSMUSP00000007959

PTM databases

iPTMnetiQ9QUI0
PhosphoSitePlusiQ9QUI0
SwissPalmiQ9QUI0

Proteomic databases

EPDiQ9QUI0
MaxQBiQ9QUI0
PaxDbiQ9QUI0
PRIDEiQ9QUI0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007959; ENSMUSP00000007959; ENSMUSG00000007815
GeneIDi11848
KEGGimmu:11848
UCSCiuc009rpe.2 mouse

Organism-specific databases

CTDi387
MGIiMGI:1096342 Rhoa

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000119020
HOGENOMiHOG000233974
HOVERGENiHBG009351
InParanoidiQ9QUI0
KOiK04513
OMAiQKIGARH
OrthoDBiEOG091G0QVS
PhylomeDBiQ9QUI0
TreeFamiTF300837

Enzyme and pathway databases

ReactomeiR-MMU-114604 GPVI-mediated activation cascade
R-MMU-193634 Axonal growth inhibition (RHOA activation)
R-MMU-194840 Rho GTPase cycle
R-MMU-198203 PI3K/AKT activation
R-MMU-209563 Axonal growth stimulation
R-MMU-2173791 TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition)
R-MMU-392451 G beta:gamma signalling through PI3Kgamma
R-MMU-3928662 EPHB-mediated forward signaling
R-MMU-3928663 EPHA-mediated growth cone collapse
R-MMU-4086400 PCP/CE pathway
R-MMU-416482 G alpha (12/13) signalling events
R-MMU-416550 Sema4D mediated inhibition of cell attachment and migration
R-MMU-416572 Sema4D induced cell migration and growth-cone collapse
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-5625740 RHO GTPases activate PKNs
R-MMU-5625900 RHO GTPases activate CIT
R-MMU-5625970 RHO GTPases activate KTN1
R-MMU-5627117 RHO GTPases Activate ROCKs
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-5666185 RHO GTPases Activate Rhotekin and Rhophilins
R-MMU-5689896 Ovarian tumor domain proteases
R-MMU-6785631 ERBB2 Regulates Cell Motility
R-MMU-6798695 Neutrophil degranulation
R-MMU-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases
R-MMU-8985586 SLIT2:ROBO1 increases RHOA activity

Miscellaneous databases

ChiTaRSiRhoa mouse
PROiPR:Q9QUI0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000007815 Expressed in 301 organ(s), highest expression level in seminal vesicle
CleanExiMM_RHOA
ExpressionAtlasiQ9QUI0 baseline and differential
GenevisibleiQ9QUI0 MM

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRHOA_MOUSE
AccessioniPrimary (citable) accession number: Q9QUI0
Secondary accession number(s): O88336
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 185 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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