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Protein

RAS guanyl-releasing protein 2

Gene

Rasgrp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.9 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi439 – 4501PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi468 – 4792PROSITE-ProRule annotationAdd BLAST12
Zinc fingeri498 – 548Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGuanine-nucleotide releasing factor
LigandCalcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-354192 Integrin alphaIIb beta3 signaling
R-MMU-392517 Rap1 signalling

Names & Taxonomyi

Protein namesi
Recommended name:
RAS guanyl-releasing protein 2
Alternative name(s):
Calcium and DAG-regulated guanine nucleotide exchange factor I
Short name:
CalDAG-GEFI
F25B3.3 kinase-like protein
Gene namesi
Name:Rasgrp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1333849 Rasgrp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Disruption phenotypei

Mice have a combination of defects in leukocytes and platelet functions which are reminiscent of the human leukocyte adhesion deficiency type III syndrome (LAD3). They display bleeding diathesis due to a defect in platelet aggregation and are resistant to collagen-induced thrombosis. In parallel, they also display impaired response to acute inflammation associated with defects in beta-1 and beta-2 integrin-mediated adhesion of neutrophils.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003156091 – 608RAS guanyl-releasing protein 2Add BLAST608

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei116PhosphoserineCombined sources1
Modified residuei117PhosphoserineCombined sources1
Modified residuei147PhosphoserineCombined sources1
Modified residuei554PhosphoserineCombined sources1
Modified residuei575PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9QUG9
PaxDbiQ9QUG9
PeptideAtlasiQ9QUG9
PRIDEiQ9QUG9

PTM databases

iPTMnetiQ9QUG9
PhosphoSitePlusiQ9QUG9

Expressioni

Tissue specificityi

Detected in megakaryocytes, platelet and neutrophils but not in lymphocytes (at protein level). Isoform 1 and isoform 3 are detected in brain basal glanglia, heart, lung, spleen, liver and kidney interstitial cells.3 Publications

Developmental stagei

Expressed in embryo with higher expression between E15 and E17.1 Publication

Gene expression databases

BgeeiENSMUSG00000032946 Expressed in 179 organ(s), highest expression level in caudate-putamen
CleanExiMM_RASGRP2
ExpressionAtlasiQ9QUG9 baseline and differential
GenevisibleiQ9QUG9 MM

Interactioni

Subunit structurei

Forms a signaling complex with RAP1 and BRAF. Interacts with F-actin (By similarity). Interacts with RAP1.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9QUG9, 1 interactor
MINTiQ9QUG9
STRINGi10090.ENSMUSP00000041135

Structurei

3D structure databases

ProteinModelPortaliQ9QUG9
SMRiQ9QUG9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 126N-terminal Ras-GEFPROSITE-ProRule annotationAdd BLAST123
Domaini154 – 387Ras-GEFPROSITE-ProRule annotationAdd BLAST234
Domaini426 – 461EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini455 – 490EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Domaini

The N-terminal Ras-GEF domain mediates association with F-actin.By similarity

Sequence similaritiesi

Belongs to the RASGRP family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri498 – 548Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG3417 Eukaryota
ENOG410XR96 LUCA
GeneTreeiENSGT00880000137870
HOGENOMiHOG000293171
HOVERGENiHBG007513
InParanoidiQ9QUG9
KOiK12361
OMAiMISYFLR
OrthoDBiEOG091G03RN
PhylomeDBiQ9QUG9
TreeFamiTF312918

Family and domain databases

CDDicd00029 C1, 1 hit
cd00051 EFh, 1 hit
cd00155 RasGEF, 1 hit
cd06224 REM, 1 hit
Gene3Di1.10.840.10, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR002219 PE/DAG-bd
IPR008937 Ras-like_GEF
IPR000651 Ras-like_Gua-exchang_fac_N
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
PANTHERiPTHR23113 PTHR23113, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF13202 EF-hand_5, 2 hits
PF00617 RasGEF, 1 hit
PF00618 RasGEF_N, 1 hit
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00054 EFh, 2 hits
SM00147 RasGEF, 1 hit
SM00229 RasGEFN, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF48366 SSF48366, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits
PS50009 RASGEF_CAT, 1 hit
PS50212 RASGEF_NTER, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9QUG9-1) [UniParc]FASTAAdd to basket
Also known as: CalDAG-GEF1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTSTLDLDKG CTVEELLRGC IEAFDDSGKV RDPQLVRMFL MMHPWYIPSS
60 70 80 90 100
QLASKLLHFY QQSRKDNSNS LQMKTCHLVR YWISAFPAEF DLNPELAEQI
110 120 130 140 150
KELKALLDQE GNRRHSSLID IESVPTYKWK RQVTQRNPVE QKKRKMSLLF
160 170 180 190 200
DHLEPMELAE HLTYLEYRSF CKILFQDYHS FVTHGCTVDN PVLERFISLF
210 220 230 240 250
NSVSQWVQLM ILSKPTATQR ALVITHFVHV AERLLQLQNF NTLMAVVGGL
260 270 280 290 300
SHSSISRLKE THSHVSPDTI KLWEGLTELV TATGNYSNYR RRLAACVGFR
310 320 330 340 350
FPILGVHLKD LVALQLALPD WLDPGRTRLN GAKMRQLFCI LEELAMVTSL
360 370 380 390 400
RPPVQANPDL LSLLTVSLDQ YQTEDELYQL SLQREPRSKS SPTSPTSCTP
410 420 430 440 450
PPRPPVLEEW TSVAKPKLDQ ALVAEHIEKM VESVFRNFDV DGDGHISQEE
460 470 480 490 500
FQIIRGNFPY LSAFGDLDQN QDGCISREEM ISYFLRSSSV LGGRMGFVHN
510 520 530 540 550
FQESNSLRPV ACRHCKALIL GIYKQGLKCR ACGVNCHKQC KERLSVECRR
560 570 580 590 600
RAQSVSLEGS APSPSPTHTH HRAFSFSLPR PGRRSSRPPE IREEEVQSVE

DGVFDIHL
Length:608
Mass (Da):69,446
Last modified:January 15, 2008 - v2
Checksum:i1E6F768EF00B0468
GO
Isoform 2 (identifier: Q9QUG9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     472-608: Missing.

Note: No experimental confirmation available.
Show »
Length:471
Mass (Da):54,054
Checksum:i3C2F75238F062E1A
GO
Isoform 3 (identifier: Q9QUG9-3) [UniParc]FASTAAdd to basket
Also known as: CalDAG-GEF1b

The sequence of this isoform differs from the canonical sequence as follows:
     125-141: PTYKWKRQVTQRNPVEQ → CVGAERKGHYACYTICA
     142-608: Missing.

Note: The corresponding protein is not undetectable.
Show »
Length:141
Mass (Da):16,187
Checksum:i242C314B00AD9191
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z2F9D3Z2F9_MOUSE
RAS guanyl-releasing protein 2
Rasgrp2
231Annotation score:
D3YXD6D3YXD6_MOUSE
RAS guanyl-releasing protein 2
Rasgrp2
164Annotation score:
D6RDD3D6RDD3_MOUSE
RAS guanyl-releasing protein 2
Rasgrp2
125Annotation score:
D3YZP2D3YZP2_MOUSE
RAS guanyl-releasing protein 2
Rasgrp2
97Annotation score:
D3YZX2D3YZX2_MOUSE
RAS guanyl-releasing protein 2
Rasgrp2
95Annotation score:
D3Z3N3D3Z3N3_MOUSE
RAS guanyl-releasing protein 2
Rasgrp2
58Annotation score:
D3YZP4D3YZP4_MOUSE
RAS guanyl-releasing protein 2
Rasgrp2
60Annotation score:
D3YZP1D3YZP1_MOUSE
RAS guanyl-releasing protein 2
Rasgrp2
70Annotation score:

Sequence cautioni

The sequence BAC28797 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2T → A in AAC79697 (PubMed:9789079).Curated1
Sequence conflicti2T → A in AAD12742 (PubMed:9789079).Curated1
Sequence conflicti73M → V in AAC79697 (PubMed:9789079).Curated1
Sequence conflicti73M → V in AAD12742 (PubMed:9789079).Curated1
Sequence conflicti83I → V in AAC79697 (PubMed:9789079).Curated1
Sequence conflicti83I → V in AAD12742 (PubMed:9789079).Curated1
Sequence conflicti99Q → P in AAC79697 (PubMed:9789079).Curated1
Sequence conflicti99Q → P in AAD12742 (PubMed:9789079).Curated1
Sequence conflicti233R → K in AAC79697 (PubMed:9789079).Curated1
Sequence conflicti233R → K in AAD12742 (PubMed:9789079).Curated1
Sequence conflicti339C → S in AAC79697 (PubMed:9789079).Curated1
Sequence conflicti339C → S in AAD12742 (PubMed:9789079).Curated1
Sequence conflicti542E → D in AAC79697 (PubMed:9789079).Curated1
Sequence conflicti542E → D in AAD12742 (PubMed:9789079).Curated1
Sequence conflicti598S → T in AAC79697 (PubMed:9789079).Curated1
Sequence conflicti598S → T in AAD12742 (PubMed:9789079).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_030577125 – 141PTYKW…NPVEQ → CVGAERKGHYACYTICA in isoform 3. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_030578142 – 608Missing in isoform 3. 1 PublicationAdd BLAST467
Alternative sequenceiVSP_030579472 – 608Missing in isoform 2. 1 PublicationAdd BLAST137

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF081193 mRNA Translation: AAC79697.1
U78171 mRNA Translation: AAD12742.1
BC051474 mRNA Translation: AAH51474.1
AK034683 mRNA Translation: BAC28797.1 Different initiation.
Y12339 mRNA Translation: CAA73008.1
CCDSiCCDS37898.1 [Q9QUG9-1]
RefSeqiNP_035372.2, NM_011242.2 [Q9QUG9-1]
XP_006531748.1, XM_006531685.3 [Q9QUG9-1]
XP_006531749.1, XM_006531686.3 [Q9QUG9-1]
XP_006531750.1, XM_006531687.2 [Q9QUG9-1]
XP_006531751.1, XM_006531688.3 [Q9QUG9-1]
XP_006531752.1, XM_006531689.3 [Q9QUG9-1]
XP_006531753.1, XM_006531690.3 [Q9QUG9-1]
XP_006531754.1, XM_006531691.3 [Q9QUG9-1]
UniGeneiMm.77017

Genome annotation databases

EnsembliENSMUST00000035716; ENSMUSP00000041135; ENSMUSG00000032946 [Q9QUG9-1]
ENSMUST00000113471; ENSMUSP00000109099; ENSMUSG00000032946 [Q9QUG9-3]
ENSMUST00000113472; ENSMUSP00000109100; ENSMUSG00000032946 [Q9QUG9-3]
ENSMUST00000113475; ENSMUSP00000109103; ENSMUSG00000032946 [Q9QUG9-3]
ENSMUST00000113476; ENSMUSP00000109104; ENSMUSG00000032946 [Q9QUG9-1]
ENSMUST00000167240; ENSMUSP00000129873; ENSMUSG00000032946 [Q9QUG9-1]
ENSMUST00000200711; ENSMUSP00000144159; ENSMUSG00000106685 [Q9QUG9-1]
ENSMUST00000200777; ENSMUSP00000143871; ENSMUSG00000106685 [Q9QUG9-1]
ENSMUST00000200858; ENSMUSP00000143974; ENSMUSG00000106685 [Q9QUG9-3]
ENSMUST00000201065; ENSMUSP00000143947; ENSMUSG00000106685 [Q9QUG9-1]
ENSMUST00000201395; ENSMUSP00000144508; ENSMUSG00000106685 [Q9QUG9-3]
ENSMUST00000202484; ENSMUSP00000144241; ENSMUSG00000106685 [Q9QUG9-3]
GeneIDi19395
KEGGimmu:19395
UCSCiuc008gip.1 mouse [Q9QUG9-1]
uc008gir.1 mouse [Q9QUG9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF081193 mRNA Translation: AAC79697.1
U78171 mRNA Translation: AAD12742.1
BC051474 mRNA Translation: AAH51474.1
AK034683 mRNA Translation: BAC28797.1 Different initiation.
Y12339 mRNA Translation: CAA73008.1
CCDSiCCDS37898.1 [Q9QUG9-1]
RefSeqiNP_035372.2, NM_011242.2 [Q9QUG9-1]
XP_006531748.1, XM_006531685.3 [Q9QUG9-1]
XP_006531749.1, XM_006531686.3 [Q9QUG9-1]
XP_006531750.1, XM_006531687.2 [Q9QUG9-1]
XP_006531751.1, XM_006531688.3 [Q9QUG9-1]
XP_006531752.1, XM_006531689.3 [Q9QUG9-1]
XP_006531753.1, XM_006531690.3 [Q9QUG9-1]
XP_006531754.1, XM_006531691.3 [Q9QUG9-1]
UniGeneiMm.77017

3D structure databases

ProteinModelPortaliQ9QUG9
SMRiQ9QUG9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9QUG9, 1 interactor
MINTiQ9QUG9
STRINGi10090.ENSMUSP00000041135

PTM databases

iPTMnetiQ9QUG9
PhosphoSitePlusiQ9QUG9

Proteomic databases

EPDiQ9QUG9
PaxDbiQ9QUG9
PeptideAtlasiQ9QUG9
PRIDEiQ9QUG9

Protocols and materials databases

DNASUi19395
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035716; ENSMUSP00000041135; ENSMUSG00000032946 [Q9QUG9-1]
ENSMUST00000113471; ENSMUSP00000109099; ENSMUSG00000032946 [Q9QUG9-3]
ENSMUST00000113472; ENSMUSP00000109100; ENSMUSG00000032946 [Q9QUG9-3]
ENSMUST00000113475; ENSMUSP00000109103; ENSMUSG00000032946 [Q9QUG9-3]
ENSMUST00000113476; ENSMUSP00000109104; ENSMUSG00000032946 [Q9QUG9-1]
ENSMUST00000167240; ENSMUSP00000129873; ENSMUSG00000032946 [Q9QUG9-1]
ENSMUST00000200711; ENSMUSP00000144159; ENSMUSG00000106685 [Q9QUG9-1]
ENSMUST00000200777; ENSMUSP00000143871; ENSMUSG00000106685 [Q9QUG9-1]
ENSMUST00000200858; ENSMUSP00000143974; ENSMUSG00000106685 [Q9QUG9-3]
ENSMUST00000201065; ENSMUSP00000143947; ENSMUSG00000106685 [Q9QUG9-1]
ENSMUST00000201395; ENSMUSP00000144508; ENSMUSG00000106685 [Q9QUG9-3]
ENSMUST00000202484; ENSMUSP00000144241; ENSMUSG00000106685 [Q9QUG9-3]
GeneIDi19395
KEGGimmu:19395
UCSCiuc008gip.1 mouse [Q9QUG9-1]
uc008gir.1 mouse [Q9QUG9-2]

Organism-specific databases

CTDi10235
MGIiMGI:1333849 Rasgrp2

Phylogenomic databases

eggNOGiKOG3417 Eukaryota
ENOG410XR96 LUCA
GeneTreeiENSGT00880000137870
HOGENOMiHOG000293171
HOVERGENiHBG007513
InParanoidiQ9QUG9
KOiK12361
OMAiMISYFLR
OrthoDBiEOG091G03RN
PhylomeDBiQ9QUG9
TreeFamiTF312918

Enzyme and pathway databases

ReactomeiR-MMU-354192 Integrin alphaIIb beta3 signaling
R-MMU-392517 Rap1 signalling

Miscellaneous databases

PROiPR:Q9QUG9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032946 Expressed in 179 organ(s), highest expression level in caudate-putamen
CleanExiMM_RASGRP2
ExpressionAtlasiQ9QUG9 baseline and differential
GenevisibleiQ9QUG9 MM

Family and domain databases

CDDicd00029 C1, 1 hit
cd00051 EFh, 1 hit
cd00155 RasGEF, 1 hit
cd06224 REM, 1 hit
Gene3Di1.10.840.10, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR002219 PE/DAG-bd
IPR008937 Ras-like_GEF
IPR000651 Ras-like_Gua-exchang_fac_N
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
PANTHERiPTHR23113 PTHR23113, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF13202 EF-hand_5, 2 hits
PF00617 RasGEF, 1 hit
PF00618 RasGEF_N, 1 hit
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00054 EFh, 2 hits
SM00147 RasGEF, 1 hit
SM00229 RasGEFN, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF48366 SSF48366, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits
PS50009 RASGEF_CAT, 1 hit
PS50212 RASGEF_NTER, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGRP2_MOUSE
AccessioniPrimary (citable) accession number: Q9QUG9
Secondary accession number(s): O09004, Q80WC0, Q8BSC8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: November 7, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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