Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Entry version 82 (18 Sep 2019)
Sequence version 3 (31 May 2011)
Previous versions | rss
Help videoAdd a publicationFeedback
Protein

Replicase large subunit

Gene

rep

Organism
Potato mop-top virus (isolate Potato/Sweden/Sw) (PMTV)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Replicase large subunit: is an RNA-dependent RNA polymerase active in viral RNA replication.By similarity
Replicase small subunit: is a methyltransferase active in RNA capping and an RNA helicase. Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. Helicase region probably exhibits NTPase and RNA unwinding activities (Potential).Curated

Miscellaneous

The replicase large subunit is translated as a fusion protein by episodic readthrough of a termination codon. When readthrough of the terminator codon TGA occurs between the codons for 1303-Lys and 1305-Arg, this results in the addition of the RdRp region.

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi1014 – 1021ATPSequence analysis8

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHelicase, Hydrolase, Methyltransferase, Nucleotidyltransferase, RNA-binding, RNA-directed RNA polymerase, Transferase
Biological processViral RNA replication
LigandATP-binding, Nucleotide-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Replicase large subunit (EC:2.1.1.-, EC:2.7.7.-, EC:2.7.7.48, EC:3.6.4.13)
Alternative name(s):
RNA-directed RNA polymerase
Cleaved into the following chain:
Replicase small subunit (EC:2.1.1.-, EC:2.7.7.-, EC:3.6.4.13)
Alternative name(s):
Methyltransferase/RNA helicase
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:rep
ORF Names:ORF1-1bis
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiPotato mop-top virus (isolate Potato/Sweden/Sw) (PMTV)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri652839 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiVirusesRiboviriaVirgaviridaePomovirus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section only exists in viral entries and indicates the host(s) either as a specific organism or taxonomic group of organisms that are susceptible to be infected by a virus.<p><a href='/help/virus_host' target='_top'>More...</a></p>Virus hostiSolanum nigrum (Black nightshade) [TaxID: 4112]
Solanum tuberosum (Potato) [TaxID: 4113]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000006715 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Genome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004094531 – 1812Replicase large subunitAdd BLAST1812
ChainiPRO_50000651971 – 1303Replicase small subunitAdd BLAST1303

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
Q9QBU0

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Heterodimer of a large and a small subunit.

By similarity

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini79 – 306Alphavirus-like MTPROSITE-ProRule annotationAdd BLAST228
Domaini979 – 1140(+)RNA virus helicase ATP-bindingAdd BLAST162
Domaini1141 – 1303(+)RNA virus helicase C-terminalAdd BLAST163
Domaini1565 – 1678RdRp catalyticPROSITE-ProRule annotationAdd BLAST114

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni58 – 820MethyltransferaseAdd BLAST763
Regioni1010 – 1272HelicaseAdd BLAST263

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and domains’ section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili543 – 573Sequence analysisAdd BLAST31

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi48 – 54Poly-Lys7
Compositional biasi686 – 694Poly-Glu9

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Coiled coil

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR027351 (+)RNA_virus_helicase_core_dom
IPR002588 Alphavirus-like_MT_dom
IPR027417 P-loop_NTPase
IPR007094 RNA-dir_pol_PSvirus
IPR001788 Tymovirus_RNA-dep_RNA_pol
IPR013664 Virgavirus_MeTrfase_C

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00978 RdRP_2, 1 hit
PF01443 Viral_helicase1, 1 hit
PF01660 Vmethyltransf, 1 hit
PF08456 Vmethyltransf_C, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF52540 SSF52540, 2 hits

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51743 ALPHAVIRUS_MT, 1 hit
PS51657 PSRV_HELICASE, 1 hit
PS50507 RDRP_SSRNA_POS, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QBU0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDQSILSKIT SDDYVNAILH TSATRVGSDL HNAMCNVIVD QIKDSTEKNK
60 70 80 90 100
KKKKIDVKRN LNEDQIQLLA ELFPERRVVT SSVHRGTHSM AAAMRKIETD
110 120 130 140 150
VIFTSFPKNG VIYDIGGNWA THAKRDDGGF VHCCCPILDF RDAQRKMTRL
160 170 180 190 200
IDFNRFIDDA KVVSAEKAAV AAQIKKDCDL ISENAKKDAY DANDLNGTWF
210 220 230 240 250
CQNKFEDCVY DHSTLGDGKK EAYGMAIHSI YDIHLVDLVS AMERKKVRVL
260 270 280 290 300
KGTFLFSADI IIGKKRGELP SVNGFYIIDG ESIKYSFYDD PNCGYEHNLN
310 320 330 340 350
SLMLYVTKTF VKAAGGAVYY LELTEMRGDT MFFTITDASE ARVMGVLHDS
360 370 380 390 400
STKCLPLNKR DLVVFPLFDI DRATDELVFR EELLSREFVN RALEYAFQLK
410 420 430 440 450
DNQVTAEGLI SYFASTNNAV VIGGSARKTS EKVDPKLLPM ITTTLMVYQE
460 470 480 490 500
LQKAKQKRVL GKLKSKVKEE LTLSGILESV VHRVFGRQSL YQRGLGVFAK
510 520 530 540 550
WMQYSYGQDL VGIHDVPLYL EINDRIKLGS ALKNVNGFSL SFSELDEKVS
560 570 580 590 600
LYEEYERERQ RISDEIVSEK IGLIGEGYVK VGESSLKPKQ KVASRDGMAQ
610 620 630 640 650
WVSGECHLYN TMKCEEKPVE KPRRFSKVVL EEWISEGNCF ALNNSFGDEV
660 670 680 690 700
HWFDSLKEAC GRAWRKQVSA VTFSDAEYFD NIENTENEEL EEEEVIKTTE
710 720 730 740 750
QVEQDWPIGN LPDVDPDDSA SAQVCLTESA STSSDEDCDP MEQLIVAACE
760 770 780 790 800
RAFATKKFEE VTMDSQVVQS EEIEIVREGE HAHANLSSGE SESSSQSQEL
810 820 830 840 850
VAVGSVPLSK WAQMVEDSEV RARQCAVDHD LSWDEVKYAK MPERPEEAEG
860 870 880 890 900
DDFRTKAKRE FLWYLKCKLV ADKSTLVEIM RDFIYGQFHS GACETPKNAC
910 920 930 940 950
FLSYEKNVCG EWMFGKRYRH PSKGASSYAV RFTREDWKRA KLIKLQWKNA
960 970 980 990 1000
KAEENGEMSS DNSDKPIVPQ GETGIYLFCD ITFLMNEIPI LNRLEISFKK
1010 1020 1030 1040 1050
RVQRRAPRIT LVDGVPGCGK STYVVKEANL VNQYVVTIGR EAAEDLRERF
1060 1070 1080 1090 1100
KSERNATATQ LKRVRTVDSY LLNDTQSRAN VLHFDEALMA HAGMVYFCAD
1110 1120 1130 1140 1150
DLSARSVICQ GDSQQIPFIN RVESITLRYS KLEIDNVVEK RLTYRSPLDV
1160 1170 1180 1190 1200
ASYLTKKNFY GTSVVTSANP LVRSLKTVGP RDGMTSIYSI PKIPGTQYLT
1210 1220 1230 1240 1250
FLQSEKEEMR QYLGRGNWNV NTVHESQGKT YDNVVLCRLK ATDNEIYPGG
1260 1270 1280 1290 1300
RNSSPYMVVG VTRHRRSLVY YTKAEDKLYF DLAEMLSVQE GKLMKHLHEE
1310 1320 1330 1340 1350
GVKXRQASKY EEIMVSDNAV TVPDVGNLVD LQEMYDIAFP GNSIVDTYFD
1360 1370 1380 1390 1400
GYEVATGGLQ IDMNASLTYY PNRQMKMWKE CRGMYPMLRT AMPEKRQSGL
1410 1420 1430 1440 1450
AEGLLALNKR NMAAPKLQES VNEFEVIEST IEKAKKVFFD ESRIDNSKLE
1460 1470 1480 1490 1500
TFEGAARWWV KQSCTAQKQM LADVRTLSEI DVTSYNFMIK GDVKPKLDLS
1510 1520 1530 1540 1550
PQSEYSALQT VVYPDKIVNA LFGPIMKEIN ERIRVALKPH VIYNTRMTSD
1560 1570 1580 1590 1600
ELDPAVEFLD VREDHESVEI DFSKFDKSKT SLHIRVVIEL YKLFGLDEMI
1610 1620 1630 1640 1650
AYLWEKSQCQ TTIKDRVNGI IAQILYQQES GNCDTYGSNT WSAALSLLES
1660 1670 1680 1690 1700
LPLEKATFMI FGGDDSLIFF PKGMVIEDPC RRLASMWNFD CKLFNFKNNS
1710 1720 1730 1740 1750
FCGKFLIKVG EKYKFAPDPY KLLTKLGRKD IKNSDLLSEI FTSIGDNYKS
1760 1770 1780 1790 1800
YDDYRVLEAL NVAVMERYKL RCDVMFGLLA LKKYINSFDL FASLFSHKGR
1810
YQRVEVGRNF EW
Length:1,812
Mass (Da):206,364
Last modified:May 31, 2011 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i97AF2F9BC94D0CFF
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AJ238607 Genomic RNA Translation: CAB58364.1

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ238607 Genomic RNA Translation: CAB58364.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

Proteomic databases

PRIDEiQ9QBU0

Family and domain databases

InterProiView protein in InterPro
IPR027351 (+)RNA_virus_helicase_core_dom
IPR002588 Alphavirus-like_MT_dom
IPR027417 P-loop_NTPase
IPR007094 RNA-dir_pol_PSvirus
IPR001788 Tymovirus_RNA-dep_RNA_pol
IPR013664 Virgavirus_MeTrfase_C
PfamiView protein in Pfam
PF00978 RdRP_2, 1 hit
PF01443 Viral_helicase1, 1 hit
PF01660 Vmethyltransf, 1 hit
PF08456 Vmethyltransf_C, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51743 ALPHAVIRUS_MT, 1 hit
PS51657 PSRV_HELICASE, 1 hit
PS50507 RDRP_SSRNA_POS, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRDRP_PMTVS
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9QBU0
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: May 31, 2011
Last modified: September 18, 2019
This is version 82 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again