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UniProtKB - Q9PWE8 (TBX5_CHICK)
Protein
T-box transcription factor TBX5
Gene
TBX5
Organism
Gallus gallus (Chicken)
Status
Functioni
DNA-binding protein that regulates the transcription of several genes and is involved in heart development and limb pattern formation. May bind to the core DNA motif of promoters.
By similarityRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 63 – 238 | T-boxPROSITE-ProRule annotationBy similarityAdd BLAST | 176 |
GO - Molecular functioni
- chaperone binding Source: AgBase
- DNA binding Source: UniProtKB
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: Ensembl
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: UniProtKB
- LIM domain binding Source: AgBase
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: UniProtKB
- RNA polymerase II-specific DNA-binding transcription factor binding Source: Ensembl
- scaffold protein binding Source: AgBase
- sequence-specific DNA binding Source: UniProtKB
GO - Biological processi
- animal organ development Source: UniProtKB
- atrial septum morphogenesis Source: Ensembl
- atrioventricular bundle cell differentiation Source: Ensembl
- atrioventricular node cell development Source: Ensembl
- atrioventricular node cell fate commitment Source: Ensembl
- atrioventricular valve morphogenesis Source: Ensembl
- bundle of His cell to Purkinje myocyte communication by electrical coupling Source: Ensembl
- cardiac left ventricle formation Source: BHF-UCL
- cell-cell signaling Source: UniProtKB
- cell-cell signaling involved in cardiac conduction Source: Ensembl
- cell fate specification Source: GO_Central
- embryonic forelimb morphogenesis Source: AgBase
- embryonic limb morphogenesis Source: UniProtKB
- endocardial cushion development Source: Ensembl
- heart development Source: UniProtKB
- hindlimb morphogenesis Source: AgBase
- limb bud formation Source: AgBase
- limb development Source: AgBase
- lung development Source: Ensembl
- morphogenesis of an epithelium Source: Ensembl
- negative regulation of cardiac muscle cell proliferation Source: UniProtKB
- negative regulation of cell migration Source: UniProtKB
- negative regulation of cell population proliferation Source: UniProtKB
- pattern specification process Source: GO_Central
- pericardium development Source: UniProtKB
- positive regulation of cardiac conduction Source: Ensembl
- positive regulation of cardiac muscle cell proliferation Source: Ensembl
- positive regulation of cardioblast differentiation Source: UniProtKB
- positive regulation of gap junction assembly Source: Ensembl
- positive regulation of secondary heart field cardioblast proliferation Source: Ensembl
- positive regulation of transcription, DNA-templated Source: AgBase
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of atrial cardiac muscle cell membrane depolarization Source: Ensembl
- regulation of gene expression Source: AgBase
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to retinoic acid Source: AgBase
- ventricular septum development Source: BHF-UCL
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: T-box transcription factor TBX5Short name: T-box protein 5 |
Gene namesi | Name:TBX5 |
Organismi | Gallus gallus (Chicken) |
Taxonomic identifieri | 9031 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archelosauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galloanserae › Galliformes › Phasianidae › Phasianinae › Gallus |
Proteomesi |
|
Subcellular locationi
Cytoskeleton
- actin cytoskeleton Source: AgBase
- actin filament Source: AgBase
- stress fiber Source: AgBase
Nucleus
- nucleus Source: AgBase
Other locations
- cytoplasm Source: AgBase
- protein-containing complex Source: UniProtKB
- protein-DNA complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000262467 | 1 – 521 | T-box transcription factor TBX5Add BLAST | 521 |
Proteomic databases
PaxDbi | Q9PWE8 |
Expressioni
Developmental stagei
Early in the developing heart, uniformly expressed throughout the entire cardiac crescent. Upon formation of the linear heart tube, expressed in a graded fashion, stronger near the posterior end and weaker at the anterior end. As the heart tube loops, asymmetric expression continues; it is expressed in the presumptive left ventricle, but not the right ventricle or outflow tract. This pattern of expression is maintained in more mature hearts (PubMed:10373308). First detected in the presumptive wing and leg mesoderm respectively at around stages 14-15. As limb outgrowth proceeds, expressed throughout the mesoderm and in the wing (PubMed:9550719).2 Publications
Gene expression databases
Bgeei | ENSGALG00000008253, Expressed in heart and 3 other tissues |
ExpressionAtlasi | Q9PWE8, baseline and differential |
Interactioni
Subunit structurei
Monomer. Homodimer (via the T-box); binds DNA as homodimer.
By similarityBinary interactionsi
Q9PWE8
With | #Exp. | IntAct |
---|---|---|
PDLIM7 [Q679P3] | 4 | EBI-6663870,EBI-6693710 |
GO - Molecular functioni
- chaperone binding Source: AgBase
- LIM domain binding Source: AgBase
- RNA polymerase II-specific DNA-binding transcription factor binding Source: Ensembl
- scaffold protein binding Source: AgBase
Protein-protein interaction databases
IntActi | Q9PWE8, 1 interactor |
STRINGi | 9031.ENSGALP00000013419 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 45 | DisorderedSequence analysisAdd BLAST | 45 | |
Regioni | 254 – 281 | DisorderedSequence analysisAdd BLAST | 28 | |
Regioni | 332 – 352 | DisorderedSequence analysisAdd BLAST | 21 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 28 – 45 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 262 – 281 | Polar residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 332 – 346 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Domaini
The T-Box domain binds to double-stranded DNA.By similarity
Phylogenomic databases
eggNOGi | KOG3585, Eukaryota |
GeneTreei | ENSGT00940000156506 |
HOGENOMi | CLU_037025_1_0_1 |
InParanoidi | Q9PWE8 |
OMAi | GEHSYKK |
OrthoDBi | 344556at2759 |
PhylomeDBi | Q9PWE8 |
TreeFami | TF106341 |
Family and domain databases
CDDi | cd00182, TBOX, 1 hit |
Gene3Di | 2.60.40.820, 1 hit |
InterProi | View protein in InterPro IPR008967, p53-like_TF_DNA-bd IPR036960, T-box_sf IPR001699, TF_T-box IPR018186, TF_T-box_CS |
PANTHERi | PTHR11267, PTHR11267, 1 hit |
Pfami | View protein in Pfam PF00907, T-box, 1 hit |
PRINTSi | PR00937, TBOX |
SMARTi | View protein in SMART SM00425, TBOX, 1 hit |
SUPFAMi | SSF49417, SSF49417, 1 hit |
PROSITEi | View protein in PROSITE PS01283, TBOX_1, 1 hit PS01264, TBOX_2, 1 hit PS50252, TBOX_3, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9PWE8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADTEEGFGL PSTPVDSEAK ELQAEAKQDP QLGTTSKAPT SPQAAFTQQG
60 70 80 90 100
MEGIKVFLHE RELWLKFHEV GTEMIITKAG RRMFPSYKVK VTGLNPKTKY
110 120 130 140 150
ILLMDIVPAD DHRYKFADNK WSVTGKAEPA MPGRLYVHPD SPATGAHWMR
160 170 180 190 200
QLVSFQKLKL TNNHLDPFGH IILNSMHKYQ PRLHIVKADE NNGFGSKNTA
210 220 230 240 250
FCTHVFPETA FIAVTSYQNH KITQLKIENN PFAKGFRGSD DMELHRMSRM
260 270 280 290 300
QSKEYPVVPR STVRQKVSSN HSPFSGETRV LSTSSNLGSQ YQCENGVSST
310 320 330 340 350
SQDLLPPTNP YPISQEHSQI YHCTKRKDEE CSTTEHPYKK PYMETSPAEE
360 370 380 390 400
DPFYRSSYPQ QQGLNTSYRT ESAQRQACMY ASSAPPTDPV PSLEDISCNT
410 420 430 440 450
WPSVPSYSSC TVSAMQPMDR LPYQHFSAHF TSGPLMPRLS SVANHTSPQI
460 470 480 490 500
GDTHSMFQHQ TSVSHQPIVR QCGPQTGIQS PPSSLQPAEF LYSHGVPRTL
510 520
SPHQYHSVHG VGMVPEWSEN S
Sequence cautioni
The sequence AAC41299 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF033671 mRNA Translation: AAC41299.1 Frameshift. AF069396 mRNA Translation: AAC23683.2 |
RefSeqi | NP_989504.1, NM_204173.1 XP_015150318.1, XM_015294832.1 |
Genome annotation databases
Ensembli | ENSGALT00000013434; ENSGALP00000013419; ENSGALG00000008253 |
GeneIDi | 373988 |
KEGGi | gga:373988 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF033671 mRNA Translation: AAC41299.1 Frameshift. AF069396 mRNA Translation: AAC23683.2 |
RefSeqi | NP_989504.1, NM_204173.1 XP_015150318.1, XM_015294832.1 |
3D structure databases
SMRi | Q9PWE8 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q9PWE8, 1 interactor |
STRINGi | 9031.ENSGALP00000013419 |
Proteomic databases
PaxDbi | Q9PWE8 |
Genome annotation databases
Ensembli | ENSGALT00000013434; ENSGALP00000013419; ENSGALG00000008253 |
GeneIDi | 373988 |
KEGGi | gga:373988 |
Organism-specific databases
CTDi | 6910 |
Phylogenomic databases
eggNOGi | KOG3585, Eukaryota |
GeneTreei | ENSGT00940000156506 |
HOGENOMi | CLU_037025_1_0_1 |
InParanoidi | Q9PWE8 |
OMAi | GEHSYKK |
OrthoDBi | 344556at2759 |
PhylomeDBi | Q9PWE8 |
TreeFami | TF106341 |
Miscellaneous databases
PROi | PR:Q9PWE8 |
Gene expression databases
Bgeei | ENSGALG00000008253, Expressed in heart and 3 other tissues |
ExpressionAtlasi | Q9PWE8, baseline and differential |
Family and domain databases
CDDi | cd00182, TBOX, 1 hit |
Gene3Di | 2.60.40.820, 1 hit |
InterProi | View protein in InterPro IPR008967, p53-like_TF_DNA-bd IPR036960, T-box_sf IPR001699, TF_T-box IPR018186, TF_T-box_CS |
PANTHERi | PTHR11267, PTHR11267, 1 hit |
Pfami | View protein in Pfam PF00907, T-box, 1 hit |
PRINTSi | PR00937, TBOX |
SMARTi | View protein in SMART SM00425, TBOX, 1 hit |
SUPFAMi | SSF49417, SSF49417, 1 hit |
PROSITEi | View protein in PROSITE PS01283, TBOX_1, 1 hit PS01264, TBOX_2, 1 hit PS50252, TBOX_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TBX5_CHICK | |
Accessioni | Q9PWE8Primary (citable) accession number: Q9PWE8 Secondary accession number(s): O73719 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2006 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |