UniProtKB - Q9PUT6 (PTEN_XENLA)
Protein
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Gene
pten
Organism
Xenopus laevis (African clawed frog)
Status
Functioni
Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement (By similarity).By similarity
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + phosphateBy similarityEC:3.1.3.67By similarity
- EC:3.1.3.16By similarity
- EC:3.1.3.16By similarity
- EC:3.1.3.48By similarity
Cofactori
Mg2+By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 123 | Phosphocysteine intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity Source: UniProtKB
- lipid binding Source: UniProtKB-KW
- PDZ domain binding Source: UniProtKB
- phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity Source: UniProtKB
- phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity Source: UniProtKB
- phosphatidylinositol-3-phosphatase activity Source: UniProtKB
- protein serine/threonine phosphatase activity Source: UniProtKB
- protein serine phosphatase activity Source: UniProtKB-EC
- protein threonine phosphatase activity Source: UniProtKB-EC
- protein tyrosine/serine/threonine phosphatase activity Source: InterPro
- protein tyrosine phosphatase activity Source: UniProtKB
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- inositol phosphate dephosphorylation Source: UniProtKB
- negative regulation of cell migration Source: UniProtKB
- negative regulation of cell population proliferation Source: UniProtKB
- negative regulation of focal adhesion assembly Source: UniProtKB
- negative regulation of protein kinase B signaling Source: UniProtKB
- nervous system development Source: UniProtKB-KW
- phosphatidylinositol dephosphorylation Source: UniProtKB
- protein dephosphorylation Source: UniProtKB
- protein kinase B signaling Source: UniProtKB
- regulation of neuron projection development Source: UniProtKB
- regulation of protein stability Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Biological process | Apoptosis, Lipid metabolism, Neurogenesis |
Ligand | Lipid-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ptenImported |
Organismi | Xenopus laevis (African clawed frog) |
Taxonomic identifieri | 8355 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus |
Organism-specific databases
Xenbasei | XB-GENE-491437, pten.L |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000227539 | 1 – 402 | Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTENAdd BLAST | 402 |
Post-translational modificationi
Monoubiquitinated. Deubiquitinated (By similarity).By similarity
Keywords - PTMi
Ubl conjugationInteractioni
Subunit structurei
Monomer.
By similarityGO - Molecular functioni
- PDZ domain binding Source: UniProtKB
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 14 – 184 | Phosphatase tensin-typePROSITE-ProRule annotationAdd BLAST | 171 | |
Domaini | 189 – 349 | C2 tensin-typePROSITE-ProRule annotationAdd BLAST | 161 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 400 – 402 | PDZ-binding | 3 |
Sequence similaritiesi
Belongs to the PTEN phosphatase protein family.Curated
Phylogenomic databases
OrthoDBi | 639380at2759 |
Family and domain databases
Gene3Di | 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR017361, Bifunc_PIno_P3_Pase/Pase_PTEN IPR000340, Dual-sp_phosphatase_cat-dom IPR029021, Prot-tyrosine_phosphatase-like IPR014020, Tensin_C2-dom IPR029023, Tensin_phosphatase IPR016130, Tyr_Pase_AS IPR003595, Tyr_Pase_cat |
Pfami | View protein in Pfam PF00782, DSPc, 1 hit PF10409, PTEN_C2, 1 hit |
PIRSFi | PIRSF038025, PTEN, 1 hit |
SMARTi | View protein in SMART SM01326, PTEN_C2, 1 hit SM00404, PTPc_motif, 1 hit |
SUPFAMi | SSF52799, SSF52799, 1 hit |
PROSITEi | View protein in PROSITE PS51182, C2_TENSIN, 1 hit PS51181, PPASE_TENSIN, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9PUT6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTAIIKEFVS RNKRRYQEDG FDLDLTYIYP NIIAMGFPAE RLEGVYRNNI
60 70 80 90 100
DDVVRFLDSK HKNHYKIYNL CAERHYDTNK FSCRVAQYPF EDHNPPQLEL
110 120 130 140 150
IKPFCEDLDQ LLSENENVAA IHCKAGKGRT GVMICAYLLH RGKFPRAQEA
160 170 180 190 200
LDFYGEVRTR DKKGVTIPSQ RRYVYYYSYL LKNSLEYRPV PLLFHKIEFE
210 220 230 240 250
TIPMFSGSTC NPQFVVYQLK VKIFTSTAGP KRAEKLMYFD FPQPLPVCGD
260 270 280 290 300
IKVEFFHKQN KVMKKEKMFH FWVNTFFIPG PEEYSEKVEN GTLVGEQELD
310 320 330 340 350
GIYSTERSDN DKEYLTLALT KNDLDKANKD KANRLFSPNF KVKLFFTKTV
360 370 380 390 400
EESSNSEASS STSVTPDVSD NEPDHYRYSD TTDSDPENEP FDEDQITQIT
KV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF144732 mRNA Translation: AAD46165.1 |
RefSeqi | NP_001083831.1, NM_001090362.1 |
Genome annotation databases
GeneIDi | 399142 |
KEGGi | xla:399142 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF144732 mRNA Translation: AAD46165.1 |
RefSeqi | NP_001083831.1, NM_001090362.1 |
3D structure databases
SMRi | Q9PUT6 |
ModBasei | Search... |
Genome annotation databases
GeneIDi | 399142 |
KEGGi | xla:399142 |
Organism-specific databases
CTDi | 399142 |
Xenbasei | XB-GENE-491437, pten.L |
Phylogenomic databases
OrthoDBi | 639380at2759 |
Family and domain databases
Gene3Di | 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR017361, Bifunc_PIno_P3_Pase/Pase_PTEN IPR000340, Dual-sp_phosphatase_cat-dom IPR029021, Prot-tyrosine_phosphatase-like IPR014020, Tensin_C2-dom IPR029023, Tensin_phosphatase IPR016130, Tyr_Pase_AS IPR003595, Tyr_Pase_cat |
Pfami | View protein in Pfam PF00782, DSPc, 1 hit PF10409, PTEN_C2, 1 hit |
PIRSFi | PIRSF038025, PTEN, 1 hit |
SMARTi | View protein in SMART SM01326, PTEN_C2, 1 hit SM00404, PTPc_motif, 1 hit |
SUPFAMi | SSF52799, SSF52799, 1 hit |
PROSITEi | View protein in PROSITE PS51182, C2_TENSIN, 1 hit PS51181, PPASE_TENSIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PTEN_XENLA | |
Accessioni | Q9PUT6Primary (citable) accession number: Q9PUT6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 7, 2006 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |