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Protein

Occludin

Gene

ocln

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Occludin
Gene namesi
Name:ocln
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-866529 ocln

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 47CytoplasmicSequence analysisAdd BLAST47
Transmembranei48 – 70HelicalSequence analysisAdd BLAST23
Topological domaini71 – 116ExtracellularSequence analysisAdd BLAST46
Transmembranei117 – 141HelicalSequence analysisAdd BLAST25
Topological domaini142 – 151CytoplasmicSequence analysis10
Transmembranei152 – 176HelicalSequence analysisAdd BLAST25
Topological domaini177 – 224ExtracellularSequence analysisAdd BLAST48
Transmembranei225 – 246HelicalSequence analysisAdd BLAST22
Topological domaini247 – 493CytoplasmicSequence analysisAdd BLAST247

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001467431 – 493OccludinAdd BLAST493

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi197 ↔ 218PROSITE-ProRule annotation
Modified residuei375Phosphothreonine; by CK2; in vitro1 Publication1
Modified residuei379Phosphoserine; by CK2; in vitro1 Publication1

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein

PTM databases

iPTMnetiQ9PUN1

Expressioni

Tissue specificityi

Localized at tight junctions of both epithelial and endothelial cells.

Developmental stagei

A maternally synthesized protein. Found in granules in the peripheral cytoplasm in the fertilized egg, it localizes first to the basolateral membrane, then to tight junctions after cingulin and ZO-1. Nascent tight junctions are in place by the two-cell stage. The maternal form is more highly phosphorylated than the form detected in later developmental stages.

Interactioni

Subunit structurei

Interacts in vitro with cingulin, possibly directly. Interacts with ZO-1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
cgnQ9PTD72EBI-79607,EBI-79525

Protein-protein interaction databases

IntActiQ9PUN1, 1 interactor

Structurei

3D structure databases

ProteinModelPortaliQ9PUN1
SMRiQ9PUN1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 250MARVELPROSITE-ProRule annotationAdd BLAST210

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili396 – 428Sequence analysisAdd BLAST33

Domaini

The C-terminus is cytoplasmic and is important for interaction with ZO-1. Necessary for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity).By similarity

Sequence similaritiesi

Belongs to the ELL/occludin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG004523
KOiK06088

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR031176 ELL/occludin
IPR008253 Marvel
IPR020846 MFS_dom
IPR002958 Occludin
IPR010844 Occludin_ELL
PANTHERiPTHR23288 PTHR23288, 1 hit
PTHR23288:SF4 PTHR23288:SF4, 1 hit
PfamiView protein in Pfam
PF01284 MARVEL, 1 hit
PF07303 Occludin_ELL, 1 hit
PIRSFiPIRSF005993 Occludin, 1 hit
PRINTSiPR01258 OCCLUDIN
PROSITEiView protein in PROSITE
PS51225 MARVEL, 1 hit

Sequencei

Sequence statusi: Complete.

Q9PUN1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MYSRPSNYAP SKDVYGGEMR SQPAYSYYPE EEIQHFYRWS SPPGIIKIMS
60 70 80 90 100
ILIVVMCVGI FACVASTLPW DLDITGQSMG YGMGSGSYSG GYTGYGFGGS
110 120 130 140 150
QMGLGFAYGG NYTDPRAAKG FILAMAAFCF IIGLVIFVML VTRTPLSTSR
160 170 180 190 200
KFYLIVIIVS AIIGGLVFIA TIVYTVGVNP VAQASGSAFY TQIVSICNQF
210 220 230 240 250
YSPVQTGVFV NQYLYHYCVV EPQEAIAIVL GFLIVVAFAI IIFFAVKTRK
260 270 280 290 300
KINQYGKTNI LWKKNHIYED GDPQVEQWVK NVAENSAPAL SDYNEKVDGS
310 320 330 340 350
VADYRSANGV QAYPSQNNIS HPIAEEELPL KEDYGMSPRH YSSSSDATTK
360 370 380 390 400
KAPPKKRPGK PRRSDLDTNE GGYNTGGESA DELEDDSWDS EYPPITQTKQ
410 420 430 440 450
RQEYKQEFAS DLHEYKRLQA ELDELSKIPV PSLNRELGQS SRKDSEEYRT
460 470 480 490
VADKYNRLKE IKSSADYRNK KKRCKGLKTK LNHIKQMVSN YDK
Length:493
Mass (Da):55,112
Last modified:May 1, 2000 - v1
Checksum:i9694CD302BEBBEDE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF170275 mRNA Translation: AAD53725.1
RefSeqiNP_001081943.1, NM_001088474.1
UniGeneiXl.544

Genome annotation databases

GeneIDi398133
KEGGixla:398133

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF170275 mRNA Translation: AAD53725.1
RefSeqiNP_001081943.1, NM_001088474.1
UniGeneiXl.544

3D structure databases

ProteinModelPortaliQ9PUN1
SMRiQ9PUN1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9PUN1, 1 interactor

PTM databases

iPTMnetiQ9PUN1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi398133
KEGGixla:398133

Organism-specific databases

CTDi398133
XenbaseiXB-GENE-866529 ocln

Phylogenomic databases

HOVERGENiHBG004523
KOiK06088

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR031176 ELL/occludin
IPR008253 Marvel
IPR020846 MFS_dom
IPR002958 Occludin
IPR010844 Occludin_ELL
PANTHERiPTHR23288 PTHR23288, 1 hit
PTHR23288:SF4 PTHR23288:SF4, 1 hit
PfamiView protein in Pfam
PF01284 MARVEL, 1 hit
PF07303 Occludin_ELL, 1 hit
PIRSFiPIRSF005993 Occludin, 1 hit
PRINTSiPR01258 OCCLUDIN
PROSITEiView protein in PROSITE
PS51225 MARVEL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiOCLN_XENLA
AccessioniPrimary (citable) accession number: Q9PUN1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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