ICL1

Functionⁱ

Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle. Required for growth on ethanol or acetate, but dispensable when fermentable carbon sources are available. Acts also on 2-methylisocitrate.By similarity

Catalytic activityⁱ

Isocitrate = succinate + glyoxylate.By similarity

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate.By similarity

Cofactorⁱ

Mg²⁺By similarity

Pathwayⁱ: glyoxylate cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

Isocitrate lyase (ICL1)
Malate synthase (MLS1)

This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	172	MagnesiumBy similarity	1
Active siteⁱ	210	Proton acceptorBy similarity	1
Binding siteⁱ	247	SubstrateBy similarity	1
Binding siteⁱ	464	SubstrateBy similarity	1

GO - Molecular functionⁱ

isocitrate lyase activity Source: UniProtKB-EC
metal ion binding Source: UniProtKB-KW
methylisocitrate lyase activity Source: UniProtKB-EC

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

glyoxylate cycle Source: UniProtKB-UniPathway
tricarboxylic acid cycle Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Lyase
Biological process	Glyoxylate bypass, Tricarboxylic acid cycle
Ligand	Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAⁱ	4.1.3.1 1096
UniPathwayⁱ	UPA00703;UER00719

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Isocitrate lyase1 Publication (EC:4.1.3.1By similarity) Short name: ICLCurated Short name: IsocitraseCurated Short name: IsocitrataseCurated Alternative name(s): Methylisocitrate lyaseBy similarity (EC:4.1.3.30By similarity) Short name: MICACurated Threo-D(S)-isocitrate glyoxylate-lyaseCurated
Gene namesⁱ	Name:ICL11 Publication
Organismⁱ	Candida albicans (Yeast)
Taxonomic identifierⁱ	5476 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Candida/Lodderomyces clade › Candida

Keywords - Cellular componentⁱ

Glyoxysome, Peroxisome

Pathology & Biotechⁱ

Chemistry databases

ChEMBLⁱ	CHEMBL5359

ChEMBLⁱ

CHEMBL5359

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000068784	1 – 550	Isocitrate lyaseAdd BLAST		550

Interactionⁱ

Subunit structureⁱ

Homotetramer.By similarity

Chemistry databases

BindingDBⁱ

Q9P8Q7

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9P8Q7
SMRⁱ	Q9P8Q7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	101 – 103	Substrate bindingBy similarity	3
Regionⁱ	211 – 212	Substrate bindingBy similarity	2
Regionⁱ	430 – 434	Substrate bindingBy similarity	5

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	548 – 550	Microbody targeting signalSequence analysis		3

Sequence similaritiesⁱ

Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.Curated

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00377 ICL_PEPM, 1 hit
InterProⁱ	View protein in InterPro IPR039556 ICL/PEPM IPR006254 Isocitrate_lyase IPR018523 Isocitrate_lyase_ph_CS IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PANTHERⁱ	PTHR21631 PTHR21631, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00463 ICL, 1 hit
PIRSFⁱ	PIRSF001362 Isocit_lyase, 1 hit
SUPFAMⁱ	SSF51621 SSF51621, 1 hit
TIGRFAMsⁱ	TIGR01346 isocit_lyase, 1 hit
PROSITEⁱ	View protein in PROSITE PS00161 ISOCITRATE_LYASE, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9P8Q7-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MPYTPIDIQK EEADFQKEVA EIKKWWSEPR WRKTKRIYSA EDIAKKRGTL 
        60         70         80         90        100
KINHPSSQQA DKLFKLLETH DADKTVSFTF GALDPIHVAQ MAKYLDSIYV 
       110        120        130        140        150
SGWQCSSTAS TSNEPSPDLA DYPMDTVPNK VEHLWFAQLF HDRKQREERL 
       160        170        180        190        200
TLSKEERAKT PYIDFLRPII ADADTGHGGI TAIIKLTKMF IERGAAGIHI 
       210        220        230        240        250
EDQAPGTKKC GHMAGKVLVP VQEHINRLVA IRASADIFGS NLLAVARTDS 
       260        270        280        290        300
EAATLITSTI DHRDHYFIIG ATNPEAGDLA ALMAEAESKG IYGNELAAIE 
       310        320        330        340        350
SEWTKKAGLK LFHEAVIDEI KNGNYSNKDA LIKKFTDKVN PLSHTSHKEA 
       360        370        380        390        400
KKLAKELTGK DIYFNWDVAR AREGYYRYQG GTQCAVMRGR AFAPYADLIW 
       410        420        430        440        450
MESALPDYAQ AKEFADGVKA AVPDQWLAYN LSPSFNWNKA MPADEQETYI 
       460        470        480        490        500
KRLGKLGYVW QFITLAGLHT TALAVDDFSN QYSQIGMKAY GQTVQQPEIE 
       510        520        530        540        550
KGVEVVKHQK WSGATYIDGL LKMVSGGVTS TAAMGQGVTE DQFKESKAKA

Length:550

Mass (Da):61,395

Last modified:October 1, 2000 - v1

Checksum:ⁱFFBDB59FB60BE337

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF222905 Genomic DNA Translation: AAF34690.1

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

AF222905 Genomic DNA Translation: AAF34690.1

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9P8Q7	Isocitrate lyase		CANTR		550	UniRef90_Q9P8Q7
Isocitrate lyase		Candida viswanathii		550
Isocitrate lyase		CANAL		550
Isocitrate lyase		CANAW		550
Isocitrate lyase		CANTT		550
+5

Protein	Similar proteins		Species	Score	Length	Source
Q9P8Q7	Isocitrate lyase	)	EMENI		538	UniRef50_P28298
Isocitrate lyase		CANGA		553
Isocitrate lyase		COPCI		537
Isocitrate lyase		COPC7		538
Isocitrate lyase		NEUCR		548
+579

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF222905 Genomic DNA Translation: AAF34690.1

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

AF222905 Genomic DNA Translation: AAF34690.1

3D structure databases

ProteinModelPortalⁱ	Q9P8Q7
SMRⁱ	Q9P8Q7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Chemistry databases

BindingDBⁱ	Q9P8Q7
ChEMBLⁱ	CHEMBL5359

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Enzyme and pathway databases

UniPathwayⁱ	UPA00703;UER00719
BRENDAⁱ	4.1.3.1 1096

Family and domain databases

CDDⁱ	cd00377 ICL_PEPM, 1 hit
InterProⁱ	View protein in InterPro IPR039556 ICL/PEPM IPR006254 Isocitrate_lyase IPR018523 Isocitrate_lyase_ph_CS IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PANTHERⁱ	PTHR21631 PTHR21631, 1 hit
Pfamⁱ	View protein in Pfam PF00463 ICL, 1 hit
PIRSFⁱ	PIRSF001362 Isocit_lyase, 1 hit
SUPFAMⁱ	SSF51621 SSF51621, 1 hit
TIGRFAMsⁱ	TIGR01346 isocit_lyase, 1 hit
PROSITEⁱ	View protein in PROSITE PS00161 ISOCITRATE_LYASE, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ACEA_CANAX
Accessionⁱ	Q9P8Q7Primary (citable) accession number: Q9P8Q7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
	Last sequence update:	October 1, 2000
	Last modified:	November 7, 2018
	This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Documents

SIMILARITY comments
Index of protein domains and families
PATHWAY comments
Index of metabolic and biosynthesis pathways

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9P8Q7 (ACEA_CANAX)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Isocitrate lyase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glyoxylate cycle

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Peroxisome

Peroxisome

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Chemistry databases

Protocols and materials databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

Pathwayⁱ: glyoxylate cycle

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ