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Protein

Mediator of replication checkpoint protein 1

Gene

mrc1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the replisome and is required for rad3-dependent activation of the checkpoint kinase cds1 in response to replication fork arrest. Phosphorylation allows it to mediate the activation of cds1.1 Publication

GO - Molecular functioni

  • 3'-flap-structured DNA binding Source: PomBase
  • bubble DNA binding Source: PomBase
  • D-loop DNA binding Source: PomBase
  • double-stranded DNA binding Source: PomBase
  • protein binding, bridging Source: PomBase
  • Y-form DNA binding Source: PomBase

GO - Biological processi

Keywordsi

Biological processDNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of replication checkpoint protein 1
Alternative name(s):
DNA replication checkpoint mediator mrc1
Gene namesi
Name:mrc1
ORF Names:SPAC694.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC694.06c
PomBaseiSPAC694.06c mrc1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi599S → A: Severe hydroxyurea (HU) sensitivity. 1 Publication1
Mutagenesisi604S → A: Severe hydroxyurea (HU) sensitivity. 1 Publication1
Mutagenesisi614S → A: Severe hydroxyurea (HU) sensitivity. 1 Publication1
Mutagenesisi634T → A: Hydroxyurea (HU) sensitivity. 1 Publication1
Mutagenesisi637S → A: Hydroxyurea (HU) sensitivity. 1 Publication1
Mutagenesisi645T → A: Hydroxyurea (HU) sensitivity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000965731 – 1019Mediator of replication checkpoint protein 1Add BLAST1019

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei604Phosphoserine1 Publication1
Modified residuei645Phosphothreonine1 Publication1

Post-translational modificationi

Phosphorylated by rad3 and tel1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9P7T4
PaxDbiQ9P7T4
PRIDEiQ9P7T4

PTM databases

iPTMnetiQ9P7T4

Interactioni

Subunit structurei

Interacts with cds1.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi279668, 122 interactors
ELMiQ9P7T4
IntActiQ9P7T4, 1 interactor
MINTiQ9P7T4
STRINGi4896.SPAC694.06c.1

Family & Domainsi

Phylogenomic databases

InParanoidiQ9P7T4
KOiK11272
OMAiYQIKDQK
OrthoDBiEOG092C303J
PhylomeDBiQ9P7T4

Family and domain databases

InterProiView protein in InterPro
IPR018564 Repl_chkpnt_MRC1_dom
PfamiView protein in Pfam
PF09444 MRC1, 1 hit

Sequencei

Sequence statusi: Complete.

Q9P7T4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASLDENADE LHRMDSSDEA SINDDQEDIL DTPRTRVRKM LASVDMQLSS
60 70 80 90 100
NAVSEASLDK ESTVGNLENQ KNRSYSSEIY LHSDTNFLSN FDSAYERVRR
110 120 130 140 150
LLNQQGGKSS LQKKEVEQIE TQEGGDNAKG SPSSENKDSD RNSRLQQLIE
160 170 180 190 200
KKRNALKKEQ EDLIQNSATS HSKSDNLDSE SADDSDLADE SELSKKYTSD
210 220 230 240 250
RKIRNASKKA LLELHRNTAR LTRETALKPE VVVKKKVTLR EFFQKIGFKN
260 270 280 290 300
DNQLENKAIS EEEANSTEPP NVEKEEPKPS VDRSTGIVNS EDIKELSVED
310 320 330 340 350
DSLELKEITP EALDIGQTSL FTTLNQTQVK KEDNKKFLLK EINAKLNEDD
360 370 380 390 400
IDSELEIEVK PKTTALDNIE KSKLSEENEH GIKGKLKQLA EIKLSKDGKP
410 420 430 440 450
FENEFNIKSF NRNLVKRAAV MAKLQRNQLE EELKAKGIYK PTIQGEKEEE
460 470 480 490 500
EDPLERARND AEKIRQLEKA SGNASDEGEL NDEEEVISSS NTPSTKAKTT
510 520 530 540 550
NKVIISDVII EATQAEPKRR QKNSRVVFDE EDLTGDSHGS SNMKISESDD
560 570 580 590 600
ESNGDMIRDS FDRLSSESIK DSQKTEELHD SFGINDEVDQ STSLYVQNSQ
610 620 630 640 650
PSASQLTIVD ATYSQPPPRW ESSSRDDKTN TSSTQPSQVD SLVPTQLDST
660 670 680 690 700
IPTQIDSVQR NKDQDDEEIL EERRESRRDS KTFLSRTMLY NKDTGKADSA
710 720 730 740 750
WASDLIEEQA IESDDEYAGI GGLSDDGLSD SDAELEVQNM IDDETTIQKG
760 770 780 790 800
EVASMAQFAK DQEMDRDEKL VKQLMKDVTT GALRKRNRNG FAALDDSDDE
810 820 830 840 850
DYSNLRREKL KELRRQKLLE DGNLNVLEGD KRKAFLATVE DSLVSSKDNL
860 870 880 890 900
TWLDATVEDS GVGSSDLGDE YLYSEQSLNH EEEEQMEEEL SEIFSSGGPN
910 920 930 940 950
VVDRVYLKKS STRHTSDNNS LEEVLPIFPG VRKLVSNSQS EKIGDLSNDN
960 970 980 990 1000
SMGAKSYKTP IISSTQRPQG RKFRGLMNQS SKADISRTVD AGSIKVVPNS
1010
QSANPPRLLA SLNNYSDFD
Length:1,019
Mass (Da):114,319
Last modified:October 1, 2000 - v1
Checksum:i6B5E7A4B5ABE5380
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAB71844.1
PIRiT50251
RefSeqiNP_594486.1, NM_001019915.2

Genome annotation databases

EnsemblFungiiSPAC694.06c.1; SPAC694.06c.1:pep; SPAC694.06c
GeneIDi2543240
KEGGispo:SPAC694.06c

Similar proteinsi

Entry informationi

Entry nameiMRC1_SCHPO
AccessioniPrimary (citable) accession number: Q9P7T4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: October 1, 2000
Last modified: July 18, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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