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Protein

Protein IMPACT

Gene

IMPACT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment and glucose deprivation. Plays a role as a negative regulator of the EIF2AK4/GCN2 kinase activity; impairs GCN1-mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis. May be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis. Through its inhibitory action on EIF2AK4/GCN2, plays a role in differentiation of neuronal cells by stimulating neurite outgrowth.By similarity

Miscellaneous

In contrast to the mouse or rabbit ortholog, the IMPACT locus is not imprinted in human.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Repressor
Biological processDifferentiation, Neurogenesis, Stress response, Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein IMPACT
Alternative name(s):
Imprinted and ancient gene protein homolog
Gene namesi
Name:IMPACT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

EuPathDBiHostDB:ENSG00000154059.9
HGNCiHGNC:20387 IMPACT
MIMi615319 gene
neXtProtiNX_Q9P2X3

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi55364
OpenTargetsiENSG00000154059
PharmGKBiPA143485502

Polymorphism and mutation databases

BioMutaiIMPACT
DMDMi296434540

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003308501 – 320Protein IMPACTAdd BLAST320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei297PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9P2X3
MaxQBiQ9P2X3
PaxDbiQ9P2X3
PeptideAtlasiQ9P2X3
PRIDEiQ9P2X3
ProteomicsDBi83909
83910 [Q9P2X3-2]

PTM databases

iPTMnetiQ9P2X3
PhosphoSitePlusiQ9P2X3

Expressioni

Tissue specificityi

Widely expressed. Expressed at high level in brain.2 Publications

Gene expression databases

BgeeiENSG00000154059
CleanExiHS_IMPACT
ExpressionAtlasiQ9P2X3 baseline and differential
GenevisibleiQ9P2X3 HS

Organism-specific databases

HPAiHPA041045
HPA041968

Interactioni

Subunit structurei

Interacts with GCN1; prevents the interaction of GCN1 with EIF2AK4/GCN2 and inhibits EIF2AK4/GCN2 kinase activity. Interaction with RPL39; this interaction occurs in a GCN1-independent manner. Associates with ribosomes; this interaction occurs in a GCN1-independent manner. Associates with actin; this interaction occurs in a GCN1-independent manner.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi120642, 35 interactors
IntActiQ9P2X3, 29 interactors
STRINGi9606.ENSP00000284202

Structurei

3D structure databases

ProteinModelPortaliQ9P2X3
SMRiQ9P2X3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 116RWDPROSITE-ProRule annotationAdd BLAST103

Sequence similaritiesi

Belongs to the IMPACT family.Curated

Phylogenomic databases

eggNOGiKOG3299 Eukaryota
COG1739 LUCA
GeneTreeiENSGT00390000017571
HOGENOMiHOG000160430
HOVERGENiHBG108005
InParanoidiQ9P2X3
OMAiIFCIKIS
OrthoDBiEOG091G0J0V
PhylomeDBiQ9P2X3
TreeFamiTF314823

Family and domain databases

Gene3Di3.10.110.10, 1 hit
3.30.230.30, 1 hit
InterProiView protein in InterPro
IPR023582 Impact
IPR001498 Impact_N
IPR036956 Impact_N_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR006575 RWD-domain
IPR016135 UBQ-conjugating_enzyme/RWD
IPR020569 UPF0029_Impact_CS
PANTHERiPTHR16301 PTHR16301, 1 hit
PfamiView protein in Pfam
PF05773 RWD, 1 hit
PF01205 UPF0029, 1 hit
SMARTiView protein in SMART
SM00591 RWD, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50908 RWD, 1 hit
PS00910 UPF0029, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9P2X3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEGDAGSDQ RQNEEIEAMA AIYGEEWCVI DDCAKIFCIR ISDDIDDPKW
60 70 80 90 100
TLCLQVMLPN EYPGTAPPIY QLNAPWLKGQ ERADLSNSLE EIYIQNIGES
110 120 130 140 150
ILYLWVEKIR DVLIQKSQMT EPGPDVKKKT EEEDVECEDD LILACQPESS
160 170 180 190 200
LKALDFDISE TRTEVEVEEL PPIDHGIPIT DRRSTFQAHL APVVCPKQVK
210 220 230 240 250
MVLSKLYENK KIASATHNIY AYRIYCEDKQ TFLQDCEDDG ETAAGGRLLH
260 270 280 290 300
LMEILNVKNV MVVVSRWYGG ILLGPDRFKH INNCARNILV EKNYTNSPEE
310 320
SSKALGKNKK VRKDKKRNEH
Length:320
Mass (Da):36,476
Last modified:May 18, 2010 - v2
Checksum:i75AAB91E00594212
GO
Isoform 2 (identifier: Q9P2X3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-118: Missing.

Note: No experimental confirmation available.
Show »
Length:202
Mass (Da):22,999
Checksum:i921D01A97FA48495
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_07784474A → V1 PublicationCorresponds to variant dbSNP:rs544203385Ensembl.1
Natural variantiVAR_042723125D → E. Corresponds to variant dbSNP:rs582234Ensembl.1
Natural variantiVAR_042724151L → V4 PublicationsCorresponds to variant dbSNP:rs677688Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0331361 – 118Missing in isoform 2. 1 PublicationAdd BLAST118

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF232229 Genomic DNA Translation: AAG23917.1
AB026264 mRNA Translation: BAA95160.1
AF208694 mRNA Translation: AAG35736.1
AK292533 mRNA Translation: BAF85222.1
AC007922 Genomic DNA No translation available.
AC020937 Genomic DNA No translation available.
CH471088 Genomic DNA Translation: EAX01186.1
BC034016 mRNA Translation: AAH34016.1
BC036074 mRNA Translation: AAH36074.1
CCDSiCCDS11886.1 [Q9P2X3-1]
RefSeqiNP_060909.1, NM_018439.3
UniGeneiHs.515317

Genome annotation databases

EnsembliENST00000284202; ENSP00000284202; ENSG00000154059 [Q9P2X3-1]
GeneIDi55364
KEGGihsa:55364
UCSCiuc002kvh.5 human [Q9P2X3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiIMPCT_HUMAN
AccessioniPrimary (citable) accession number: Q9P2X3
Secondary accession number(s): A8MXG0, Q49AM0, Q9H2X4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 18, 2010
Last modified: June 20, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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