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Protein

Rho GTPase-activating protein 28

Gene

ARHGAP28

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGTPase activation

Enzyme and pathway databases

ReactomeiR-HSA-194840 Rho GTPase cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 28
Alternative name(s):
Rho-type GTPase-activating protein 28
Gene namesi
Name:ARHGAP28
Synonyms:KIAA1314
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

EuPathDBiHostDB:ENSG00000088756.12
HGNCiHGNC:25509 ARHGAP28
MIMi610592 gene
neXtProtiNX_Q9P2N2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi79822
OpenTargetsiENSG00000088756
PharmGKBiPA134915320

Polymorphism and mutation databases

DMDMi311033539

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002804761 – 729Rho GTPase-activating protein 28Add BLAST729

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei72PhosphoserineBy similarity1
Modified residuei159PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9P2N2
MaxQBiQ9P2N2
PaxDbiQ9P2N2
PeptideAtlasiQ9P2N2
PRIDEiQ9P2N2
ProteomicsDBi83851
83852 [Q9P2N2-2]
83853 [Q9P2N2-3]
83854 [Q9P2N2-5]

PTM databases

iPTMnetiQ9P2N2
PhosphoSitePlusiQ9P2N2

Expressioni

Tissue specificityi

Expressed in testis. Expressed at moderate level in kidney and ovary, and weakly expressed in spleen and skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000088756
CleanExiHS_ARHGAP28
ExpressionAtlasiQ9P2N2 baseline and differential
GenevisibleiQ9P2N2 HS

Organism-specific databases

HPAiHPA030413
HPA030414
HPA030415

Interactioni

Protein-protein interaction databases

IntActiQ9P2N2, 3 interactors
STRINGi9606.ENSP00000313506

Structurei

3D structure databases

ProteinModelPortaliQ9P2N2
SMRiQ9P2N2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini380 – 577Rho-GAPPROSITE-ProRule annotationAdd BLAST198

Phylogenomic databases

eggNOGiKOG2200 Eukaryota
ENOG410XQ10 LUCA
GeneTreeiENSGT00760000119123
HOGENOMiHOG000015106
HOVERGENiHBG072023
InParanoidiQ9P2N2
OMAiRMNEATM
OrthoDBiEOG091G03X0
PhylomeDBiQ9P2N2
TreeFamiTF314044

Family and domain databases

Gene3Di1.10.555.10, 1 hit
InterProiView protein in InterPro
IPR008936 Rho_GTPase_activation_prot
IPR000198 RhoGAP_dom
PfamiView protein in Pfam
PF00620 RhoGAP, 1 hit
SMARTiView protein in SMART
SM00324 RhoGAP, 1 hit
SUPFAMiSSF48350 SSF48350, 1 hit
PROSITEiView protein in PROSITE
PS50238 RHOGAP, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9P2N2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEVEDSGGVV LTAYHSYARA QPPNAESRCA PRAAASHPLS RKSIPRCRRI
60 70 80 90 100
NRMLSNESLH PPAFSRSNSE ASVDSASMED FWREIESIKD SSMGGQEEPP
110 120 130 140 150
PAEVTPVDEG ELEAEWLQDV GLSTLISGDE EEDGKALLST LTRTQAAAVQ
160 170 180 190 200
KRYHTYTQTM RKKDKQSIRD VRDIFGVSES PPRDTCGNHT NQLDGTKEER
210 220 230 240 250
ELPRVIKTSG SMPDDASLNS TTLSDASQDK EGSFAVPRSD SVAILETIPV
260 270 280 290 300
LPVHSNGSPE PGQPVQNAIS DDDFLEKNIP PEAEELSFEV SYSEMVTEAL
310 320 330 340 350
KRNKLKKSEI KKEDYVLTKF NVQKTRFGLT EAGDLSAEDM KKIRHLSLIE
360 370 380 390 400
LTAFFDAFGI QLKRNKTEKV KGRDNGIFGV PLTVLLDGDR KKDPGVKVPL
410 420 430 440 450
VLQKFFEKVE ESGLESEGIF RLSGCTAKVK QYREELDAKF NADKFKWDKM
460 470 480 490 500
CHREAAVMLK AFFRELPTSL FPVEYIPAFI SLMERGPHVK VQFQALHLMV
510 520 530 540 550
MALPDANRDA AQALMTFFNK VIANESKNRM SLWNISTVMA PNLFFSRSKH
560 570 580 590 600
SDYEELLLAN TAAHIIRLML KYQKILWKVP SFLITQVRRM NEATMLLKKQ
610 620 630 640 650
LPSVRKLLRR KTLERETASP KTSKVLQKSP SARRMSDVPE GVIRVHAPLL
660 670 680 690 700
SKVSMAIQLN NQTKAKDILA KFQYENSHGS SECIKIQNQR LYEIGGNIGE
710 720
HCLDPDAYIL DVYRINPQAE WVIKPQQSS
Length:729
Mass (Da):82,060
Last modified:November 2, 2010 - v3
Checksum:i3272A9A651BB0CF6
GO
Isoform 2 (identifier: Q9P2N2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.
     677-729: SHGSSECIKI...EWVIKPQQSS → RILHWQRAAL...LFTIGLDIST

Show »
Length:670
Mass (Da):75,770
Checksum:i990F02D6A1A582C5
GO
Isoform 3 (identifier: Q9P2N2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-177: Missing.
     178-181: SESP → MNEL
     677-729: SHGSSECIKI...EWVIKPQQSS → RILHWQRAAL...LFTIGLDIST

Note: No experimental confirmation available.
Show »
Length:545
Mass (Da):61,956
Checksum:i3C8521D80DB18B44
GO
Isoform 5 (identifier: Q9P2N2-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-159: Missing.

Note: No experimental confirmation available.
Show »
Length:570
Mass (Da):64,615
Checksum:i47963DC9D216EAA5
GO

Sequence cautioni

The sequence BAA91533 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAA92552 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti598K → E in BX648684 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_055833168I → V. Corresponds to variant dbSNP:rs2303978Ensembl.1
Natural variantiVAR_031155190T → S. Corresponds to variant dbSNP:rs6506448Ensembl.1
Natural variantiVAR_031156727Q → P3 PublicationsCorresponds to variant dbSNP:rs1056408Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0237251 – 177Missing in isoform 3. 2 PublicationsAdd BLAST177
Alternative sequenceiVSP_0399711 – 159Missing in isoform 5. 1 PublicationAdd BLAST159
Alternative sequenceiVSP_0237261 – 52Missing in isoform 2. 1 PublicationAdd BLAST52
Alternative sequenceiVSP_023727178 – 181SESP → MNEL in isoform 3. 2 Publications4
Alternative sequenceiVSP_023728677 – 729SHGSS…PQQSS → RILHWQRAALSFLNGKWVKK EREESTETNRSPKHVFLFTI GLDIST in isoform 2 and isoform 3. 3 PublicationsAdd BLAST53

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB037735 mRNA Translation: BAA92552.1 Different initiation.
BX648684 mRNA No translation available.
AP005205 Genomic DNA No translation available.
AP005210 Genomic DNA No translation available.
BC033668 mRNA Translation: AAH33668.2
BC065274 mRNA Translation: AAH65274.1
AK001174 mRNA Translation: BAA91533.1 Different initiation.
CCDSiCCDS32785.1 [Q9P2N2-5]
PIRiE59436
UniGeneiHs.183114

Genome annotation databases

EnsembliENST00000262227; ENSP00000262227; ENSG00000088756 [Q9P2N2-2]
ENST00000314319; ENSP00000313506; ENSG00000088756 [Q9P2N2-5]
ENST00000383472; ENSP00000372964; ENSG00000088756 [Q9P2N2-1]
ENST00000419673; ENSP00000392660; ENSG00000088756 [Q9P2N2-5]
ENST00000532996; ENSP00000435990; ENSG00000088756 [Q9P2N2-3]
UCSCiuc002knc.5 human [Q9P2N2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiRHG28_HUMAN
AccessioniPrimary (citable) accession number: Q9P2N2
Secondary accession number(s): A8MQB7
, A8MU88, Q6P160, Q8N4T3, Q9NW53
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: November 2, 2010
Last modified: June 20, 2018
This is version 127 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

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