UniProtKB - Q9P2M4 (TBC14_HUMAN)
Protein
TBC1 domain family member 14
Gene
TBC1D14
Organism
Homo sapiens (Human)
Status
Functioni
Plays a role in the regulation of starvation-induced autophagosome formation (PubMed:22613832). Together with the TRAPPIII complex, regulates a constitutive trafficking step from peripheral recycling endosomes to the early Golgi, maintaining the cycling pool of ATG9 required for initiation of autophagy.2 Publications
GO - Molecular functioni
- GTPase activator activity Source: GO_Central
- protein kinase binding Source: BHF-UCL
- Rab GTPase binding Source: GO_Central
GO - Biological processi
- activation of GTPase activity Source: GO_Central
- autophagy Source: UniProtKB-KW
- intracellular protein transport Source: GO_Central
- negative regulation of autophagy Source: BHF-UCL
- recycling endosome to Golgi transport Source: UniProtKB
- regulation of autophagosome assembly Source: BHF-UCL
Keywordsi
Molecular function | GTPase activation |
Biological process | Autophagy |
Enzyme and pathway databases
PathwayCommonsi | Q9P2M4 |
Reactomei | R-HSA-8854214, TBC/RABGAPs |
Names & Taxonomyi
Protein namesi | Recommended name: TBC1 domain family member 14 |
Gene namesi | Name:TBC1D14 Synonyms:KIAA1322 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000132405.18 |
HGNCi | HGNC:29246, TBC1D14 |
MIMi | 614855, gene |
neXtProti | NX_Q9P2M4 |
Subcellular locationi
Golgi apparatus
- cis-Golgi network 2 Publications
- trans-Golgi network 1 Publication
Note: After amino acid starvation, Golgi apparatus-associated protein levels increase compared with fed conditions. May be cycling between the Golgi apparatus and an endosomal pool, redistributing to the Golgi apparatus upon starvation.
Cytosol
- cytosol Source: GOC
Endosome
- recycling endosome Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: HPA
Nucleus
- nucleoplasm Source: HPA
Other locations
- autophagosome Source: BHF-UCL
- intracellular membrane-bounded organelle Source: HPA
Keywords - Cellular componenti
Golgi apparatusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 472 | R → A: Loss of inhibition of autophagosome formation; when associated with A-508. 1 Publication | 1 | |
Mutagenesisi | 508 | Q → A: Loss of inhibition of autophagosome formation; when associated with A-472. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 57533 |
OpenTargetsi | ENSG00000132405 |
PharmGKBi | PA128394697 |
Miscellaneous databases
Pharosi | Q9P2M4, Tbio |
Polymorphism and mutation databases
BioMutai | TBC1D14 |
DMDMi | 172044690 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000208040 | 1 – 693 | TBC1 domain family member 14Add BLAST | 693 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 91 | PhosphoserineCombined sources | 1 | |
Modified residuei | 295 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9P2M4 |
jPOSTi | Q9P2M4 |
MassIVEi | Q9P2M4 |
MaxQBi | Q9P2M4 |
PaxDbi | Q9P2M4 |
PeptideAtlasi | Q9P2M4 |
PRIDEi | Q9P2M4 |
ProteomicsDBi | 83847 [Q9P2M4-1] 83848 [Q9P2M4-2] |
PTM databases
iPTMneti | Q9P2M4 |
PhosphoSitePlusi | Q9P2M4 |
Expressioni
Gene expression databases
Bgeei | ENSG00000132405, Expressed in secondary oocyte and 212 other tissues |
ExpressionAtlasi | Q9P2M4, baseline and differential |
Genevisiblei | Q9P2M4, HS |
Organism-specific databases
HPAi | ENSG00000132405, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsQ9P2M4
With | #Exp. | IntAct |
---|---|---|
NXF1 [Q9UBU9] | 3 | EBI-2797718,EBI-398874 |
RAB11B [Q15907] | 2 | EBI-2797718,EBI-722234 |
ULK1 [O75385] | 4 | EBI-2797718,EBI-908831 |
GO - Molecular functioni
- protein kinase binding Source: BHF-UCL
- Rab GTPase binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 121592, 30 interactors |
IntActi | Q9P2M4, 37 interactors |
MINTi | Q9P2M4 |
STRINGi | 9606.ENSP00000386921 |
Miscellaneous databases
RNActi | Q9P2M4, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9P2M4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9P2M4 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 401 – 611 | Rab-GAP TBCPROSITE-ProRule annotationAdd BLAST | 211 |
Phylogenomic databases
eggNOGi | KOG2223, Eukaryota |
GeneTreei | ENSGT00940000157250 |
HOGENOMi | CLU_015133_1_1_1 |
InParanoidi | Q9P2M4 |
OMAi | XNLLARK |
OrthoDBi | 798837at2759 |
PhylomeDBi | Q9P2M4 |
TreeFami | TF313318 |
Family and domain databases
InterProi | View protein in InterPro IPR000195, Rab-GTPase-TBC_dom IPR035969, Rab-GTPase_TBC_sf |
Pfami | View protein in Pfam PF00566, RabGAP-TBC, 1 hit |
SMARTi | View protein in SMART SM00164, TBC, 1 hit |
SUPFAMi | SSF47923, SSF47923, 2 hits |
PROSITEi | View protein in PROSITE PS50086, TBC_RABGAP, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9P2M4-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTDGKLSTST NGVAFMGILD GRPGNPLQNL QHVNLKAPRL LSAPEYGPKL
60 70 80 90 100
KLRALEDRHS LQSVDSGIPT LEIGNPEPVP CSAVHVRRKQ SDSDLIPERA
110 120 130 140 150
FQSACALPSC APPAPSSTER EQSVRKSSTF PRTGYDSVKL YSPTSKALTR
160 170 180 190 200
SDDVSVCSVS SLGTELSTTL SVSNEDILDL VVTSSSSAIV TLENDDDPQF
210 220 230 240 250
TNVTLSSIKE TRGLHQQDCV HEAEEGSKLK ILGPFSNFFA RNLLARKQSA
260 270 280 290 300
RLDKHNDLGW KLFGKAPLRE NAQKDSKRIQ KEYEDKAGRP SKPPSPKQNV
310 320 330 340 350
RKNLDFEPLS TTALILEDRP ANLPAKPAEE AQKHRQQYEE MVVQAKKREL
360 370 380 390 400
KEAQRRKKQL EERCRVEESI GNAVLTWNNE ILPNWETMWC SRKVRDLWWQ
410 420 430 440 450
GIPPSVRGKV WSLAIGNELN ITHELFDICL ARAKERWRSL STGGSEVENE
460 470 480 490 500
DAGFSAADRE ASLELIKLDI SRTFPNLCIF QQGGPYHDML HSILGAYTCY
510 520 530 540 550
RPDVGYVQGM SFIAAVLILN LDTADAFIAF SNLLNKPCQM AFFRVDHGLM
560 570 580 590 600
LTYFAAFEVF FEENLPKLFA HFKKNNLTPD IYLIDWIFTL YSKSLPLDLA
610 620 630 640 650
CRIWDVFCRD GEEFLFRTAL GILKLFEDIL TKMDFIHMAQ FLTRLPEDLP
660 670 680 690
AEELFASIAT IQMQSRNKKW AQVLTALQKD SREMEKGSPS LRH
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB9A071 | B9A071_HUMAN | TBC1 domain family member 14 | TBC1D14 | 465 | Annotation score: | ||
F5GXK4 | F5GXK4_HUMAN | TBC1 domain family member 14 | TBC1D14 | 353 | Annotation score: | ||
C9JP26 | C9JP26_HUMAN | TBC1 domain family member 14 | TBC1D14 | 127 | Annotation score: | ||
H7BZD7 | H7BZD7_HUMAN | TBC1 domain family member 14 | TBC1D14 | 200 | Annotation score: | ||
C9J541 | C9J541_HUMAN | TBC1 domain family member 14 | TBC1D14 | 56 | Annotation score: |
Sequence cautioni
The sequence AAH41167 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAA92560 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_067442 | 41 | L → V2 PublicationsCorresponds to variant dbSNP:rs34860182Ensembl. | 1 | |
Natural variantiVAR_059856 | 446 | E → Q. Corresponds to variant dbSNP:rs11731231Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041460 | 1 – 280 | Missing in isoform 2. CuratedAdd BLAST | 280 | |
Alternative sequenceiVSP_041461 | 281 | K → M in isoform 2. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB449900 mRNA Translation: BAH16643.1 AB037743 mRNA Translation: BAA92560.1 Different initiation. AC092463 Genomic DNA No translation available. AC097382 Genomic DNA No translation available. AC106045 Genomic DNA No translation available. CH471131 Genomic DNA Translation: EAW82375.1 CH471131 Genomic DNA Translation: EAW82376.1 CH471131 Genomic DNA Translation: EAW82377.1 BC041167 mRNA Translation: AAH41167.1 Different initiation. AL833868 mRNA Translation: CAD38726.1 |
CCDSi | CCDS3394.2 [Q9P2M4-1] CCDS47006.1 [Q9P2M4-2] |
RefSeqi | NP_001106832.1, NM_001113361.1 [Q9P2M4-1] NP_001106834.1, NM_001113363.1 [Q9P2M4-2] NP_001273734.1, NM_001286805.1 NP_001317567.1, NM_001330638.1 NP_065824.2, NM_020773.2 [Q9P2M4-1] XP_006713958.1, XM_006713895.2 [Q9P2M4-1] |
Genome annotation databases
Ensembli | ENST00000409757; ENSP00000386921; ENSG00000132405 [Q9P2M4-1] ENST00000448507; ENSP00000404041; ENSG00000132405 [Q9P2M4-1] ENST00000451522; ENSP00000388886; ENSG00000132405 [Q9P2M4-2] |
GeneIDi | 57533 |
KEGGi | hsa:57533 |
UCSCi | uc003gjs.5, human [Q9P2M4-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB449900 mRNA Translation: BAH16643.1 AB037743 mRNA Translation: BAA92560.1 Different initiation. AC092463 Genomic DNA No translation available. AC097382 Genomic DNA No translation available. AC106045 Genomic DNA No translation available. CH471131 Genomic DNA Translation: EAW82375.1 CH471131 Genomic DNA Translation: EAW82376.1 CH471131 Genomic DNA Translation: EAW82377.1 BC041167 mRNA Translation: AAH41167.1 Different initiation. AL833868 mRNA Translation: CAD38726.1 |
CCDSi | CCDS3394.2 [Q9P2M4-1] CCDS47006.1 [Q9P2M4-2] |
RefSeqi | NP_001106832.1, NM_001113361.1 [Q9P2M4-1] NP_001106834.1, NM_001113363.1 [Q9P2M4-2] NP_001273734.1, NM_001286805.1 NP_001317567.1, NM_001330638.1 NP_065824.2, NM_020773.2 [Q9P2M4-1] XP_006713958.1, XM_006713895.2 [Q9P2M4-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2QQ8 | X-ray | 2.00 | A | 372-687 | [»] | |
SMRi | Q9P2M4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 121592, 30 interactors |
IntActi | Q9P2M4, 37 interactors |
MINTi | Q9P2M4 |
STRINGi | 9606.ENSP00000386921 |
PTM databases
iPTMneti | Q9P2M4 |
PhosphoSitePlusi | Q9P2M4 |
Polymorphism and mutation databases
BioMutai | TBC1D14 |
DMDMi | 172044690 |
Proteomic databases
EPDi | Q9P2M4 |
jPOSTi | Q9P2M4 |
MassIVEi | Q9P2M4 |
MaxQBi | Q9P2M4 |
PaxDbi | Q9P2M4 |
PeptideAtlasi | Q9P2M4 |
PRIDEi | Q9P2M4 |
ProteomicsDBi | 83847 [Q9P2M4-1] 83848 [Q9P2M4-2] |
Protocols and materials databases
Antibodypediai | 43347, 100 antibodies |
Genome annotation databases
Ensembli | ENST00000409757; ENSP00000386921; ENSG00000132405 [Q9P2M4-1] ENST00000448507; ENSP00000404041; ENSG00000132405 [Q9P2M4-1] ENST00000451522; ENSP00000388886; ENSG00000132405 [Q9P2M4-2] |
GeneIDi | 57533 |
KEGGi | hsa:57533 |
UCSCi | uc003gjs.5, human [Q9P2M4-1] |
Organism-specific databases
CTDi | 57533 |
DisGeNETi | 57533 |
EuPathDBi | HostDB:ENSG00000132405.18 |
GeneCardsi | TBC1D14 |
HGNCi | HGNC:29246, TBC1D14 |
HPAi | ENSG00000132405, Low tissue specificity |
MIMi | 614855, gene |
neXtProti | NX_Q9P2M4 |
OpenTargetsi | ENSG00000132405 |
PharmGKBi | PA128394697 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2223, Eukaryota |
GeneTreei | ENSGT00940000157250 |
HOGENOMi | CLU_015133_1_1_1 |
InParanoidi | Q9P2M4 |
OMAi | XNLLARK |
OrthoDBi | 798837at2759 |
PhylomeDBi | Q9P2M4 |
TreeFami | TF313318 |
Enzyme and pathway databases
PathwayCommonsi | Q9P2M4 |
Reactomei | R-HSA-8854214, TBC/RABGAPs |
Miscellaneous databases
BioGRID-ORCSi | 57533, 4 hits in 843 CRISPR screens |
ChiTaRSi | TBC1D14, human |
EvolutionaryTracei | Q9P2M4 |
GenomeRNAii | 57533 |
Pharosi | Q9P2M4, Tbio |
PROi | PR:Q9P2M4 |
RNActi | Q9P2M4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000132405, Expressed in secondary oocyte and 212 other tissues |
ExpressionAtlasi | Q9P2M4, baseline and differential |
Genevisiblei | Q9P2M4, HS |
Family and domain databases
InterProi | View protein in InterPro IPR000195, Rab-GTPase-TBC_dom IPR035969, Rab-GTPase_TBC_sf |
Pfami | View protein in Pfam PF00566, RabGAP-TBC, 1 hit |
SMARTi | View protein in SMART SM00164, TBC, 1 hit |
SUPFAMi | SSF47923, SSF47923, 2 hits |
PROSITEi | View protein in PROSITE PS50086, TBC_RABGAP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TBC14_HUMAN | |
Accessioni | Q9P2M4Primary (citable) accession number: Q9P2M4 Secondary accession number(s): B9A6L5 Q8NDK3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 21, 2004 |
Last sequence update: | February 26, 2008 | |
Last modified: | December 2, 2020 | |
This is version 151 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations