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Protein

Cleavage and polyadenylation specificity factor subunit 2

Gene

CPSF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Involved in the histone 3' end pre-mRNA processing.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing

Enzyme and pathway databases

ReactomeiR-HSA-109688 Cleavage of Growing Transcript in the Termination Region
R-HSA-159231 Transport of Mature mRNA Derived from an Intronless Transcript
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72187 mRNA 3'-end processing
R-HSA-77595 Processing of Intronless Pre-mRNAs

Names & Taxonomyi

Protein namesi
Recommended name:
Cleavage and polyadenylation specificity factor subunit 2
Alternative name(s):
Cleavage and polyadenylation specificity factor 100 kDa subunit
Short name:
CPSF 100 kDa subunit
Gene namesi
Name:CPSF2
Synonyms:CPSF100, KIAA1367
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000165934.12
HGNCiHGNC:2325 CPSF2
MIMi606028 gene
neXtProtiNX_Q9P2I0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi67H → A: Inhibits histone 3'-end processing. 1 Publication1
Mutagenesisi289D → A: Does not inhibit histone 3'-end processing. 1 Publication1
Mutagenesisi543R → A: Inhibits histone 3'-end processing. 1 Publication1

Organism-specific databases

DisGeNETi53981
OpenTargetsiENSG00000165934
PharmGKBiPA26842

Polymorphism and mutation databases

BioMutaiCPSF2
DMDMi51338827

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000743931 – 782Cleavage and polyadenylation specificity factor subunit 2Add BLAST782

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei419PhosphoserineCombined sources1
Modified residuei420PhosphoserineCombined sources1
Modified residuei423PhosphoserineCombined sources1
Modified residuei660PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9P2I0
MaxQBiQ9P2I0
PaxDbiQ9P2I0
PeptideAtlasiQ9P2I0
PRIDEiQ9P2I0
ProteomicsDBi83819

PTM databases

iPTMnetiQ9P2I0
PhosphoSitePlusiQ9P2I0

Expressioni

Gene expression databases

BgeeiENSG00000165934 Expressed in 205 organ(s), highest expression level in amniotic fluid
CleanExiHS_CPSF2
ExpressionAtlasiQ9P2I0 baseline and differential
GenevisibleiQ9P2I0 HS

Organism-specific databases

HPAiHPA024238

Interactioni

Subunit structurei

Component of the cleavage and polyadenylation specificity factor (CPSF) complex, composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1. Interacts with CPSF3, CSTF2 and SYMPK. Interacts with ZC3H3 (By similarity).By similarity3 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi119826, 62 interactors
CORUMiQ9P2I0
DIPiDIP-42500N
IntActiQ9P2I0, 23 interactors
MINTiQ9P2I0
STRINGi9606.ENSP00000298875

Structurei

3D structure databases

ProteinModelPortaliQ9P2I0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1135 Eukaryota
COG1236 LUCA
GeneTreeiENSGT00910000144260
HOGENOMiHOG000264343
HOVERGENiHBG051106
InParanoidiQ9P2I0
KOiK14402
OMAiWKNKESG
OrthoDBiEOG091G03GG
PhylomeDBiQ9P2I0
TreeFamiTF106131

Family and domain databases

CDDicd16293 CPSF2-like_MBL-fold, 1 hit
Gene3Di3.60.15.10, 1 hit
InterProiView protein in InterPro
IPR022712 Beta_Casp
IPR027075 CPSF2
IPR025069 Cpsf2_C
IPR035639 CPSF2_MBL
IPR001279 Metallo-B-lactamas
IPR036866 RibonucZ/Hydroxyglut_hydro
IPR011108 RMMBL
PANTHERiPTHR11203:SF5 PTHR11203:SF5, 1 hit
PfamiView protein in Pfam
PF10996 Beta-Casp, 1 hit
PF13299 CPSF100_C, 1 hit
PF16661 Lactamase_B_6, 1 hit
PF07521 RMMBL, 1 hit
SMARTiView protein in SMART
SM01027 Beta-Casp, 1 hit
SM00849 Lactamase_B, 1 hit
SUPFAMiSSF56281 SSF56281, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9P2I0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTSIIKLTTL SGVQEESALC YLLQVDEFRF LLDCGWDEHF SMDIIDSLRK
60 70 80 90 100
HVHQIDAVLL SHPDPLHLGA LPYAVGKLGL NCAIYATIPV YKMGQMFMYD
110 120 130 140 150
LYQSRHNTED FTLFTLDDVD AAFDKIQQLK FSQIVNLKGK GHGLSITPLP
160 170 180 190 200
AGHMIGGTIW KIVKDGEEEI VYAVDFNHKR EIHLNGCSLE MLSRPSLLIT
210 220 230 240 250
DSFNATYVQP RRKQRDEQLL TNVLETLRGD GNVLIAVDTA GRVLELAQLL
260 270 280 290 300
DQIWRTKDAG LGVYSLALLN NVSYNVVEFS KSQVEWMSDK LMRCFEDKRN
310 320 330 340 350
NPFQFRHLSL CHGLSDLARV PSPKVVLASQ PDLECGFSRD LFIQWCQDPK
360 370 380 390 400
NSIILTYRTT PGTLARFLID NPSEKITEIE LRKRVKLEGK ELEEYLEKEK
410 420 430 440 450
LKKEAAKKLE QSKEADIDSS DESDIEEDID QPSAHKTKHD LMMKGEGSRK
460 470 480 490 500
GSFFKQAKKS YPMFPAPEER IKWDEYGEII KPEDFLVPEL QATEEEKSKL
510 520 530 540 550
ESGLTNGDEP MDQDLSDVPT KCISTTESIE IKARVTYIDY EGRSDGDSIK
560 570 580 590 600
KIINQMKPRQ LIIVHGPPEA SQDLAECCRA FGGKDIKVYM PKLHETVDAT
610 620 630 640 650
SETHIYQVRL KDSLVSSLQF CKAKDAELAW IDGVLDMRVS KVDTGVILEE
660 670 680 690 700
GELKDDGEDS EMQVEAPSDS SVIAQQKAMK SLFGDDEKET GEESEIIPTL
710 720 730 740 750
EPLPPHEVPG HQSVFMNEPR LSDFKQVLLR EGIQAEFVGG VLVCNNQVAV
760 770 780
RRTETGRIGL EGCLCQDFYR IRDLLYEQYA IV
Length:782
Mass (Da):88,487
Last modified:August 16, 2004 - v2
Checksum:iF67B4813B9883CE8
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V3T7G3V3T7_HUMAN
Cleavage and polyadenylation specif...
CPSF2
80Annotation score:
G3V5T3G3V5T3_HUMAN
Cleavage and polyadenylation specif...
CPSF2
62Annotation score:
H0YJF4H0YJF4_HUMAN
Cleavage and polyadenylation specif...
CPSF2
269Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti289D → G in AAH70095 (PubMed:15489334).Curated1
Sequence conflicti654K → R in AAH70095 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001627 mRNA Translation: BAG50953.1
CH471061 Genomic DNA Translation: EAW81480.1
BC070095 mRNA Translation: AAH70095.1
AB037788 mRNA Translation: BAA92605.1
AL442079 mRNA Translation: CAC09445.1
CCDSiCCDS9902.1
RefSeqiNP_001309201.1, NM_001322272.1
NP_059133.1, NM_017437.2
UniGeneiHs.657632
Hs.736541

Genome annotation databases

EnsembliENST00000298875; ENSP00000298875; ENSG00000165934
GeneIDi53981
KEGGihsa:53981
UCSCiuc001yah.3 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001627 mRNA Translation: BAG50953.1
CH471061 Genomic DNA Translation: EAW81480.1
BC070095 mRNA Translation: AAH70095.1
AB037788 mRNA Translation: BAA92605.1
AL442079 mRNA Translation: CAC09445.1
CCDSiCCDS9902.1
RefSeqiNP_001309201.1, NM_001322272.1
NP_059133.1, NM_017437.2
UniGeneiHs.657632
Hs.736541

3D structure databases

ProteinModelPortaliQ9P2I0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119826, 62 interactors
CORUMiQ9P2I0
DIPiDIP-42500N
IntActiQ9P2I0, 23 interactors
MINTiQ9P2I0
STRINGi9606.ENSP00000298875

PTM databases

iPTMnetiQ9P2I0
PhosphoSitePlusiQ9P2I0

Polymorphism and mutation databases

BioMutaiCPSF2
DMDMi51338827

Proteomic databases

EPDiQ9P2I0
MaxQBiQ9P2I0
PaxDbiQ9P2I0
PeptideAtlasiQ9P2I0
PRIDEiQ9P2I0
ProteomicsDBi83819

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000298875; ENSP00000298875; ENSG00000165934
GeneIDi53981
KEGGihsa:53981
UCSCiuc001yah.3 human

Organism-specific databases

CTDi53981
DisGeNETi53981
EuPathDBiHostDB:ENSG00000165934.12
GeneCardsiCPSF2
HGNCiHGNC:2325 CPSF2
HPAiHPA024238
MIMi606028 gene
neXtProtiNX_Q9P2I0
OpenTargetsiENSG00000165934
PharmGKBiPA26842
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1135 Eukaryota
COG1236 LUCA
GeneTreeiENSGT00910000144260
HOGENOMiHOG000264343
HOVERGENiHBG051106
InParanoidiQ9P2I0
KOiK14402
OMAiWKNKESG
OrthoDBiEOG091G03GG
PhylomeDBiQ9P2I0
TreeFamiTF106131

Enzyme and pathway databases

ReactomeiR-HSA-109688 Cleavage of Growing Transcript in the Termination Region
R-HSA-159231 Transport of Mature mRNA Derived from an Intronless Transcript
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72187 mRNA 3'-end processing
R-HSA-77595 Processing of Intronless Pre-mRNAs

Miscellaneous databases

ChiTaRSiCPSF2 human
GeneWikiiCPSF2
GenomeRNAii53981
PROiPR:Q9P2I0
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000165934 Expressed in 205 organ(s), highest expression level in amniotic fluid
CleanExiHS_CPSF2
ExpressionAtlasiQ9P2I0 baseline and differential
GenevisibleiQ9P2I0 HS

Family and domain databases

CDDicd16293 CPSF2-like_MBL-fold, 1 hit
Gene3Di3.60.15.10, 1 hit
InterProiView protein in InterPro
IPR022712 Beta_Casp
IPR027075 CPSF2
IPR025069 Cpsf2_C
IPR035639 CPSF2_MBL
IPR001279 Metallo-B-lactamas
IPR036866 RibonucZ/Hydroxyglut_hydro
IPR011108 RMMBL
PANTHERiPTHR11203:SF5 PTHR11203:SF5, 1 hit
PfamiView protein in Pfam
PF10996 Beta-Casp, 1 hit
PF13299 CPSF100_C, 1 hit
PF16661 Lactamase_B_6, 1 hit
PF07521 RMMBL, 1 hit
SMARTiView protein in SMART
SM01027 Beta-Casp, 1 hit
SM00849 Lactamase_B, 1 hit
SUPFAMiSSF56281 SSF56281, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCPSF2_HUMAN
AccessioniPrimary (citable) accession number: Q9P2I0
Secondary accession number(s): B3KME1, Q6NSJ1, Q9H3W7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: August 16, 2004
Last modified: September 12, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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