UniProtKB - Q9P2H5 (UBP35_HUMAN)
Protein
Ubiquitin carboxyl-terminal hydrolase 35
Gene
USP35
Organism
Homo sapiens (Human)
Status
Functioni
Catalytic activityi
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 450 | NucleophilePROSITE-ProRule annotation | 1 | |
Active sitei | 862 | Proton acceptorPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- cysteine-type endopeptidase activity Source: GO_Central
- thiol-dependent ubiquitin-specific protease activity Source: FlyBase
GO - Biological processi
- protein deubiquitination Source: FlyBase
- ubiquitin-dependent protein catabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
PathwayCommonsi | Q9P2H5 |
Protein family/group databases
MEROPSi | C19.059 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin carboxyl-terminal hydrolase 35 (EC:3.4.19.12)Alternative name(s): Deubiquitinating enzyme 35 Ubiquitin thioesterase 35 Ubiquitin-specific-processing protease 35 |
Gene namesi | Name:USP35 Synonyms:KIAA1372, USP34 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:20061, USP35 |
neXtProti | NX_Q9P2H5 |
VEuPathDBi | HostDB:ENSG00000118369.12 |
Subcellular locationi
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 57558 |
OpenTargetsi | ENSG00000118369 |
PharmGKBi | PA134883706 |
Miscellaneous databases
Pharosi | Q9P2H5, Tbio |
Genetic variation databases
BioMutai | USP35 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080665 | 1 – 1018 | Ubiquitin carboxyl-terminal hydrolase 35Add BLAST | 1018 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 613 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9P2H5 |
jPOSTi | Q9P2H5 |
MassIVEi | Q9P2H5 |
MaxQBi | Q9P2H5 |
PaxDbi | Q9P2H5 |
PeptideAtlasi | Q9P2H5 |
PRIDEi | Q9P2H5 |
ProteomicsDBi | 83817 [Q9P2H5-1] 83818 [Q9P2H5-2] |
PTM databases
iPTMneti | Q9P2H5 |
PhosphoSitePlusi | Q9P2H5 |
Expressioni
Tissue specificityi
Expressed in testis, pancreas and skeletal muscle.1 Publication
Gene expression databases
Bgeei | ENSG00000118369, Expressed in secondary oocyte and 150 other tissues |
ExpressionAtlasi | Q9P2H5, baseline and differential |
Genevisiblei | Q9P2H5, HS |
Organism-specific databases
HPAi | ENSG00000118369, Tissue enhanced (parathyroid) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 121613, 10 interactors |
IntActi | Q9P2H5, 3 interactors |
STRINGi | 9606.ENSP00000431876 |
Miscellaneous databases
RNActi | Q9P2H5, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9P2H5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 441 – 926 | USPAdd BLAST | 486 |
Sequence similaritiesi
Belongs to the peptidase C19 family.Curated
Phylogenomic databases
eggNOGi | KOG1864, Eukaryota |
GeneTreei | ENSGT00940000160942 |
HOGENOMi | CLU_010910_0_0_1 |
InParanoidi | Q9P2H5 |
OMAi | GPHYLIL |
OrthoDBi | 802596at2759 |
PhylomeDBi | Q9P2H5 |
TreeFami | TF324529 |
Family and domain databases
InterProi | View protein in InterPro IPR016024, ARM-type_fold IPR038765, Papain-like_cys_pep_sf IPR001394, Peptidase_C19_UCH IPR018200, USP_CS IPR028889, USP_dom |
Pfami | View protein in Pfam PF00443, UCH, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00972, USP_1, 1 hit PS00973, USP_2, 1 hit PS50235, USP_3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9P2H5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDKILEAVVT SSYPVSVKQG LVRRVLEAAR QPLEREQCLA LLALGARLYV
60 70 80 90 100
GGAEELPRRV GCQLLHVAGR HHPDVFAEFF SARRVLRLLQ GGAGPPGPRA
110 120 130 140 150
LACVQLGLQL LPEGPAADEV FALLRREVLR TVCERPGPAA CAQVARLLAR
160 170 180 190 200
HPRCVPDGPH RLLFCQQLVR CLGRFRCPAE GEEGAVEFLE QAQQVSGLLA
210 220 230 240 250
QLWRAQPAAI LPCLKELFAV ISCAEEEPPS SALASVVQHL PLELMDGVVR
260 270 280 290 300
NLSNDDSVTD SQMLTAISRM IDWVSWPLGK NIDKWIIALL KGLAAVKKFS
310 320 330 340 350
ILIEVSLTKI EKVFSKLLYP IVRGAALSVL KYMLLTFQHS HEAFHLLLPH
360 370 380 390 400
IPPMVASLVK EDSNSGTSCL EQLAELVHCM VFRFPGFPDL YEPVMEAIKD
410 420 430 440 450
LHVPNEDRIK QLLGQDAWTS QKSELAGFYP RLMAKSDTGK IGLINLGNTC
460 470 480 490 500
YVNSILQALF MASDFRHCVL RLTENNSQPL MTKLQWLFGF LEHSQRPAIS
510 520 530 540 550
PENFLSASWT PWFSPGTQQD CSEYLKYLLD RLHEEEKTGT RICQKLKQSS
560 570 580 590 600
SPSPPEEPPA PSSTSVEKMF GGKIVTRICC LCCLNVSSRE EAFTDLSLAF
610 620 630 640 650
PPPERCRRRR LGSVMRPTED ITARELPPPT SAQGPGRVGP RRQRKHCITE
660 670 680 690 700
DTPPTSLYIE GLDSKEAGGQ SSQEERIERE EEGKEERTEK EEVGEEEEST
710 720 730 740 750
RGEGEREKEE EVEEEEEKVE KETEKEAEQE KEEDSLGAGT HPDAAIPSGE
760 770 780 790 800
RTCGSEGSRS VLDLVNYFLS PEKLTAENRY YCESCASLQD AEKVVELSQG
810 820 830 840 850
PCYLILTLLR FSFDLRTMRR RKILDDVSIP LLLRLPLAGG RGQAYDLCSV
860 870 880 890 900
VVHSGVSSES GHYYCYAREG AARPAASLGT ADRPEPENQW YLFNDTRVSF
910 920 930 940 950
SSFESVSNVT SFFPKDTAYV LFYRQRPREG PEAELGSSRV RTEPTLHKDL
960 970 980 990 1000
MEAISKDNIL YLQEQEKEAR SRAAYISALP TSPHWGRGFD EDKDEDEGSP
1010
GGCNPAGGNG GDFHRLVF
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PK78 | E9PK78_HUMAN | Ubiquitin carboxyl-terminal hydrola... | USP35 | 586 | Annotation score: | ||
E9PRM2 | E9PRM2_HUMAN | Ubiquitin carboxyl-terminal hydrola... | USP35 | 298 | Annotation score: | ||
H0YEP1 | H0YEP1_HUMAN | Ubiquitin carboxyl-terminal hydrola... | USP35 | 108 | Annotation score: |
Sequence cautioni
The sequence BAA92610 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 224 | Missing in CAE51937 (PubMed:14715245).Curated | 1 | |
Sequence conflicti | 464 | D → E in CAE51937 (PubMed:14715245).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_057042 | 236 | V → M. Corresponds to variant dbSNP:rs2510044Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_040291 | 1 – 269 | Missing in isoform 2. 1 PublicationAdd BLAST | 269 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ586137 mRNA Translation: CAE51937.1 AB037793 mRNA Translation: BAA92610.1 Different initiation. AP002985 Genomic DNA No translation available. |
CCDSi | CCDS41693.1 [Q9P2H5-1] |
RefSeqi | NP_065849.1, NM_020798.2 [Q9P2H5-1] XP_011543489.1, XM_011545187.2 [Q9P2H5-2] XP_011543490.1, XM_011545188.2 [Q9P2H5-2] XP_016873541.1, XM_017018052.1 |
Genome annotation databases
Ensembli | ENST00000526425; ENSP00000434942; ENSG00000118369 [Q9P2H5-2] ENST00000529308; ENSP00000431876; ENSG00000118369 [Q9P2H5-1] |
GeneIDi | 57558 |
KEGGi | hsa:57558 |
UCSCi | uc001ozf.4, human [Q9P2H5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ586137 mRNA Translation: CAE51937.1 AB037793 mRNA Translation: BAA92610.1 Different initiation. AP002985 Genomic DNA No translation available. |
CCDSi | CCDS41693.1 [Q9P2H5-1] |
RefSeqi | NP_065849.1, NM_020798.2 [Q9P2H5-1] XP_011543489.1, XM_011545187.2 [Q9P2H5-2] XP_011543490.1, XM_011545188.2 [Q9P2H5-2] XP_016873541.1, XM_017018052.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5TXK | X-ray | 1.84 | A | 423-944 | [»] | |
SMRi | Q9P2H5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 121613, 10 interactors |
IntActi | Q9P2H5, 3 interactors |
STRINGi | 9606.ENSP00000431876 |
Protein family/group databases
MEROPSi | C19.059 |
PTM databases
iPTMneti | Q9P2H5 |
PhosphoSitePlusi | Q9P2H5 |
Genetic variation databases
BioMutai | USP35 |
Proteomic databases
EPDi | Q9P2H5 |
jPOSTi | Q9P2H5 |
MassIVEi | Q9P2H5 |
MaxQBi | Q9P2H5 |
PaxDbi | Q9P2H5 |
PeptideAtlasi | Q9P2H5 |
PRIDEi | Q9P2H5 |
ProteomicsDBi | 83817 [Q9P2H5-1] 83818 [Q9P2H5-2] |
Protocols and materials databases
Antibodypediai | 31304, 76 antibodies |
Genome annotation databases
Ensembli | ENST00000526425; ENSP00000434942; ENSG00000118369 [Q9P2H5-2] ENST00000529308; ENSP00000431876; ENSG00000118369 [Q9P2H5-1] |
GeneIDi | 57558 |
KEGGi | hsa:57558 |
UCSCi | uc001ozf.4, human [Q9P2H5-1] |
Organism-specific databases
CTDi | 57558 |
DisGeNETi | 57558 |
GeneCardsi | USP35 |
HGNCi | HGNC:20061, USP35 |
HPAi | ENSG00000118369, Tissue enhanced (parathyroid) |
neXtProti | NX_Q9P2H5 |
OpenTargetsi | ENSG00000118369 |
PharmGKBi | PA134883706 |
VEuPathDBi | HostDB:ENSG00000118369.12 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1864, Eukaryota |
GeneTreei | ENSGT00940000160942 |
HOGENOMi | CLU_010910_0_0_1 |
InParanoidi | Q9P2H5 |
OMAi | GPHYLIL |
OrthoDBi | 802596at2759 |
PhylomeDBi | Q9P2H5 |
TreeFami | TF324529 |
Enzyme and pathway databases
PathwayCommonsi | Q9P2H5 |
Miscellaneous databases
BioGRID-ORCSi | 57558, 22 hits in 994 CRISPR screens |
ChiTaRSi | USP35, human |
GenomeRNAii | 57558 |
Pharosi | Q9P2H5, Tbio |
PROi | PR:Q9P2H5 |
RNActi | Q9P2H5, protein |
Gene expression databases
Bgeei | ENSG00000118369, Expressed in secondary oocyte and 150 other tissues |
ExpressionAtlasi | Q9P2H5, baseline and differential |
Genevisiblei | Q9P2H5, HS |
Family and domain databases
InterProi | View protein in InterPro IPR016024, ARM-type_fold IPR038765, Papain-like_cys_pep_sf IPR001394, Peptidase_C19_UCH IPR018200, USP_CS IPR028889, USP_dom |
Pfami | View protein in Pfam PF00443, UCH, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00972, USP_1, 1 hit PS00973, USP_2, 1 hit PS50235, USP_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBP35_HUMAN | |
Accessioni | Q9P2H5Primary (citable) accession number: Q9P2H5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 10, 2003 |
Last sequence update: | November 30, 2010 | |
Last modified: | April 7, 2021 | |
This is version 154 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families