UniProtKB - Q9P2D3 (HTR5B_HUMAN)
HEAT repeat-containing protein 5B
HEATR5B
Functioni
Component of clathrin-coated vesicles (PubMed:15758025).
Component of the aftiphilin/p200/gamma-synergin complex, which plays roles in AP1G1/AP-1-mediated protein trafficking including the trafficking of transferrin from early to recycling endosomes, and the membrane trafficking of furin and the lysosomal enzyme cathepsin D between the trans-Golgi network (TGN) and endosomes (PubMed:15758025).
1 PublicationGO - Biological processi
- endocytosis Source: GO_Central
- protein localization Source: GO_Central
- protein transport Source: UniProtKB-KW
- retrograde transport, endosome to Golgi Source: GO_Central
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q9P2D3 |
SignaLinki | Q9P2D3 |
Names & Taxonomyi
Protein namesi | Recommended name: HEAT repeat-containing protein 5B |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:29273, HEATR5B |
MIMi | 619627, gene |
neXtProti | NX_Q9P2D3 |
VEuPathDBi | HostDB:ENSG00000008869 |
Subcellular locationi
Other locations
- perinuclear region 1 Publication
- clathrin-coated vesicle 1 Publication
Note: Localization at clathrin-coated vesicles depends on AFTPH/aftiphilin.1 Publication
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear speck Source: HPA
Other locations
- clathrin-coated vesicle Source: UniProtKB-SubCell
- endocytic vesicle Source: GO_Central
- intracellular membrane-bounded organelle Source: HPA
- membrane Source: UniProtKB
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesiclePathology & Biotechi
Organism-specific databases
DisGeNETi | 54497 |
OpenTargetsi | ENSG00000008869 |
PharmGKBi | PA162390697 |
Miscellaneous databases
Pharosi | Q9P2D3, Tdark |
Genetic variation databases
BioMutai | HEATR5B |
DMDMi | 162416219 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000311994 | 1 – 2071 | HEAT repeat-containing protein 5BAdd BLAST | 2071 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1123 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1563 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 1564 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1737 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9P2D3 |
jPOSTi | Q9P2D3 |
MassIVEi | Q9P2D3 |
MaxQBi | Q9P2D3 |
PaxDbi | Q9P2D3 |
PeptideAtlasi | Q9P2D3 |
PRIDEi | Q9P2D3 |
ProteomicsDBi | 83775 [Q9P2D3-1] 83776 [Q9P2D3-2] 83777 [Q9P2D3-3] |
PTM databases
CarbonylDBi | Q9P2D3 |
iPTMneti | Q9P2D3 |
PhosphoSitePlusi | Q9P2D3 |
SwissPalmi | Q9P2D3 |
Expressioni
Gene expression databases
Bgeei | ENSG00000008869, Expressed in left ventricle myocardium and 217 other tissues |
ExpressionAtlasi | Q9P2D3, baseline and differential |
Genevisiblei | Q9P2D3, HS |
Organism-specific databases
HPAi | ENSG00000008869, Low tissue specificity |
Interactioni
Subunit structurei
Self-associates (PubMed:15758025).
Component of the aftiphilin/p200/gamma-synergin complex, at least composed of AFTPH/aftiphilin, HEATR5B/p200a and SYNRG/gamma-synergin, which plays a role in the AP1G1/AP-1-mediated protein trafficking from early to recycling endosomes and between the trans-Golgi network (TGN) and endosomes (PubMed:15758025). Within the complex interacts with AFTPH/aftiphilin and SYNRG/gamma-synergin; the interactions are direct (PubMed:15758025).
Interacts with GGA1 (PubMed:15758025).
1 PublicationBinary interactionsi
Q9P2D3
With | #Exp. | IntAct |
---|---|---|
DLG1 [Q12959] | 2 | EBI-2832021,EBI-357481 |
Protein-protein interaction databases
BioGRIDi | 119993, 33 interactors |
IntActi | Q9P2D3, 12 interactors |
STRINGi | 9606.ENSP00000233099 |
Miscellaneous databases
RNActi | Q9P2D3, protein |
Structurei
3D structure databases
AlphaFoldDBi | Q9P2D3 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 848 – 885 | HEAT 1Add BLAST | 38 | |
Repeati | 1062 – 1099 | HEAT 2Add BLAST | 38 | |
Repeati | 1290 – 1327 | HEAT 3Add BLAST | 38 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1822, Eukaryota |
GeneTreei | ENSGT00390000006205 |
HOGENOMi | CLU_000652_0_0_1 |
InParanoidi | Q9P2D3 |
OMAi | ADTHNEE |
OrthoDBi | 71064at2759 |
PhylomeDBi | Q9P2D3 |
TreeFami | TF300706 |
Family and domain databases
Gene3Di | 1.25.10.10, 3 hits |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR040108, Laa1/Sip1/HEATR5 |
PANTHERi | PTHR21663, PTHR21663, 1 hit |
SUPFAMi | SSF48371, SSF48371, 2 hits |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MELAHSLLLN EEALAQITEA KRPVFIFEWL RFLDKVLVAA NKTDVKEKQK
60 70 80 90 100
KLVEQLTGLI SSSPGPPTRK LLAKNLAALY SIGDTFTVFQ TLDKCNDIIR
110 120 130 140 150
NKDDTAAYLP TKLAAVACVG AFYEKMGRML GSAFPETVNN LLKSLKSAES
160 170 180 190 200
QGRSEILMSL QKVLSGLGGA AASSHRDIYK NARSLLTDRS MAVRCAVAKC
210 220 230 240 250
LLELQNEAVF MWTAELENIA TLCFKALENS NYGVRVAVSK LLGTVMATAL
260 270 280 290 300
MPKQATVMRQ NVKRATFDEV LELMATGFLR GGSGFLKSGG EMLKVGGSVN
310 320 330 340 350
REVRVGVTQA YVVFVTTLGG QWLERSFATF LSHVLDLVSH PRATQTHVEA
360 370 380 390 400
VYSRRCVSFI LRATVGSLLG EKAQIAAAKE ICQAIGKQMK AVEAVVNDTS
410 420 430 440 450
GENKSGAADI AASQHVMVCA LQELGSLVQS LNATASPLIQ EASIGLLEIV
460 470 480 490 500
TSVLLHPSMA ARLAAAWCLR CVAVALPFQL TPFLDRCAER LNNLKTSPEA
510 520 530 540 550
VSGYSFAMAA LLGGVHQCPL GIPHAKGKMV VSIAEDLLRT AAQNSRLSLQ
560 570 580 590 600
RTQAGWLLLG ALMTLGPSVV RYHLPKMLLL WRNVFPRSLK ELEAEKARGD
610 620 630 640 650
SFTWQVTLEG RAGALCAMRS FVAHCPELLT EDVIRKLMTP IECAMTMMSH
660 670 680 690 700
IPSVMKAHGA HLKASAAMVR LRLYDILALL PPKTYEGSFN ALLRELVAEF
710 720 730 740 750
TLTDNSANTT TSLLRSLCHY DDSVLLGSWL QETDHKSIED QLQPNSASGS
760 770 780 790 800
GALEHDPSSI YLRIPAGEAV PGPLPLGVSV IDASVALFGV VFPHVSYKHR
810 820 830 840 850
LQMLDHFAEC VKQAKGVRQQ AVQLNIFTAV LSALKGLAEN KSTLGPEEVR
860 870 880 890 900
KSALTLVMGP LDNPNPILRC AAGEALGRMA QVVGEATFIA RMAQYSFDKL
910 920 930 940 950
KSARDVVSRT GHSLALGCLH RYVGGIGSGQ HLKTSVSILL ALAQDGTSPE
960 970 980 990 1000
VQTWSLHSLA LIVDSSGPMY RGYVEPTLSL VLTLLLTVPP SHTEVHQCLG
1010 1020 1030 1040 1050
RCLGAIITTV GPELQGNGAT TSTIRSSCLV GCAITQDHSD SLVQAAAISC
1060 1070 1080 1090 1100
LQQLHMFAPR HVNLSSLVPS LCVHLCSSHL LLRRAAVACL RQLAQREAAE
1110 1120 1130 1140 1150
VCEYAMSLAK NTGDKESSSA NVSPFAPGVS SRTDIHCRHQ GVNITETGLE
1160 1170 1180 1190 1200
GLLFGMLDRE TDRKLCSDIH DTLGHMLSSL AVEKLSHWLM LCKDVLAASS
1210 1220 1230 1240 1250
DMSTATLLSS GKDEEAEKKD EMDDDTMFTT LGEEDKSKPF VAPRWATRVF
1260 1270 1280 1290 1300
AADCLCRIIN LCENADQAHF DLALARSAKL RNPTNDLLVL HLSDLIRMAF
1310 1320 1330 1340 1350
MAATDHSNQL RMAGLQALED IIKKFASVPE PEFPGHVILE QYQANVGAAL
1360 1370 1380 1390 1400
RPAFSQDTPS DIIAKACQVC STWIGSGVVS DLNDLRRVHN LLVSSLDKVQ
1410 1420 1430 1440 1450
AGKGSSSQLY RESATTMEKL AVLKAWAEVY VVAMNIKKEA ESKPKRAIKN
1460 1470 1480 1490 1500
TDDDDDDCGT IDELPPDSLI TLVQPELPTL SRLWLAALKD YALLTLPAEF
1510 1520 1530 1540 1550
SSQLPPDGGA FYTPETIDTA RLHYRNSWAP ILHAVALWLN STGFTCSEST
1560 1570 1580 1590 1600
EAAAISGLQK RSTSVNLNQA SGAVGSAKSL PEINKDRMHL ILGVSIQFLC
1610 1620 1630 1640 1650
SPRPEEPIEH VTACLQALHT LLDSPYARVH IAEDQLIGVE LLSVLHRLLL
1660 1670 1680 1690 1700
TWNPSSVQLL VTGVVQQIVR AAQDYLQEKR NTLNEDDMEK EACTVLGEGG
1710 1720 1730 1740 1750
DSGGLIPGKS LVFATMELLM FILVRHMPHL STKVSDSPSH IATKTRLSEE
1760 1770 1780 1790 1800
SARLVAATVT ILSDLPSLCS PAGCMTILPT ILFLIARILK DTAIKSADNQ
1810 1820 1830 1840 1850
VPPPVSAALQ GIKSIVTLSM AKTEAGVQKQ WTALIRSTLA CILEYSQPED
1860 1870 1880 1890 1900
SVPTPDEVSM LTAIALFLWS ASNEIIGVQS LQNGCMNRFK NALNSCDPWV
1910 1920 1930 1940 1950
QAKCYQLLLS VFQHSNRALS TPYIHSLAPI VVEKLKAVER NRPASNIELL
1960 1970 1980 1990 2000
AVQEGIKVLE TLVALGEEQN RVQLLALLVP TLISYLLDEN SFASASSASK
2010 2020 2030 2040 2050
DLHEFALQNL MHIGPLYPHA FKTVMGAAPE LKVRLETAVR ASQASKAKAA
2060 2070
ARQPAPAIHS APTIKLKTSF F
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH7C2X3 | H7C2X3_HUMAN | HEAT repeat-containing protein 5B | HEATR5B | 115 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 324 | E → G in CAD97959 (PubMed:17974005).Curated | 1 | |
Sequence conflicti | 1640 | E → G in BAA91733 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_037392 | 1601 | S → P. Corresponds to variant dbSNP:rs2302657Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_029690 | 763 – 768 | RIPAGE → LLFLVH in isoform 2. 1 Publication | 6 | |
Alternative sequenceiVSP_029691 | 769 – 2071 | Missing in isoform 2. 1 PublicationAdd BLAST | 1303 | |
Alternative sequenceiVSP_029692 | 1684 – 1773 | NEDDM…CSPAG → R in isoform 3. 1 PublicationAdd BLAST | 90 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC007404 Genomic DNA No translation available. AC007899 Genomic DNA No translation available. BX538008 mRNA Translation: CAD97959.1 AB037835 mRNA Translation: BAA92652.1 AK001513 mRNA Translation: BAA91733.1 Different initiation. |
CCDSi | CCDS33181.1 [Q9P2D3-1] |
RefSeqi | NP_061897.1, NM_019024.2 [Q9P2D3-1] XP_011531236.1, XM_011532934.2 [Q9P2D3-1] XP_016859867.1, XM_017004378.1 [Q9P2D3-3] |
Genome annotation databases
Ensembli | ENST00000233099.6; ENSP00000233099.5; ENSG00000008869.12 |
GeneIDi | 54497 |
KEGGi | hsa:54497 |
MANE-Selecti | ENST00000233099.6; ENSP00000233099.5; NM_019024.3; NP_061897.1 |
UCSCi | uc002rpp.2, human [Q9P2D3-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC007404 Genomic DNA No translation available. AC007899 Genomic DNA No translation available. BX538008 mRNA Translation: CAD97959.1 AB037835 mRNA Translation: BAA92652.1 AK001513 mRNA Translation: BAA91733.1 Different initiation. |
CCDSi | CCDS33181.1 [Q9P2D3-1] |
RefSeqi | NP_061897.1, NM_019024.2 [Q9P2D3-1] XP_011531236.1, XM_011532934.2 [Q9P2D3-1] XP_016859867.1, XM_017004378.1 [Q9P2D3-3] |
3D structure databases
AlphaFoldDBi | Q9P2D3 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 119993, 33 interactors |
IntActi | Q9P2D3, 12 interactors |
STRINGi | 9606.ENSP00000233099 |
PTM databases
CarbonylDBi | Q9P2D3 |
iPTMneti | Q9P2D3 |
PhosphoSitePlusi | Q9P2D3 |
SwissPalmi | Q9P2D3 |
Genetic variation databases
BioMutai | HEATR5B |
DMDMi | 162416219 |
Proteomic databases
EPDi | Q9P2D3 |
jPOSTi | Q9P2D3 |
MassIVEi | Q9P2D3 |
MaxQBi | Q9P2D3 |
PaxDbi | Q9P2D3 |
PeptideAtlasi | Q9P2D3 |
PRIDEi | Q9P2D3 |
ProteomicsDBi | 83775 [Q9P2D3-1] 83776 [Q9P2D3-2] 83777 [Q9P2D3-3] |
Protocols and materials databases
Antibodypediai | 52336, 18 antibodies from 8 providers |
DNASUi | 54497 |
Genome annotation databases
Ensembli | ENST00000233099.6; ENSP00000233099.5; ENSG00000008869.12 |
GeneIDi | 54497 |
KEGGi | hsa:54497 |
MANE-Selecti | ENST00000233099.6; ENSP00000233099.5; NM_019024.3; NP_061897.1 |
UCSCi | uc002rpp.2, human [Q9P2D3-1] |
Organism-specific databases
CTDi | 54497 |
DisGeNETi | 54497 |
GeneCardsi | HEATR5B |
HGNCi | HGNC:29273, HEATR5B |
HPAi | ENSG00000008869, Low tissue specificity |
MIMi | 619627, gene |
neXtProti | NX_Q9P2D3 |
OpenTargetsi | ENSG00000008869 |
PharmGKBi | PA162390697 |
VEuPathDBi | HostDB:ENSG00000008869 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1822, Eukaryota |
GeneTreei | ENSGT00390000006205 |
HOGENOMi | CLU_000652_0_0_1 |
InParanoidi | Q9P2D3 |
OMAi | ADTHNEE |
OrthoDBi | 71064at2759 |
PhylomeDBi | Q9P2D3 |
TreeFami | TF300706 |
Enzyme and pathway databases
PathwayCommonsi | Q9P2D3 |
SignaLinki | Q9P2D3 |
Miscellaneous databases
BioGRID-ORCSi | 54497, 24 hits in 1074 CRISPR screens |
ChiTaRSi | HEATR5B, human |
GenomeRNAii | 54497 |
Pharosi | Q9P2D3, Tdark |
PROi | PR:Q9P2D3 |
RNActi | Q9P2D3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000008869, Expressed in left ventricle myocardium and 217 other tissues |
ExpressionAtlasi | Q9P2D3, baseline and differential |
Genevisiblei | Q9P2D3, HS |
Family and domain databases
Gene3Di | 1.25.10.10, 3 hits |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR040108, Laa1/Sip1/HEATR5 |
PANTHERi | PTHR21663, PTHR21663, 1 hit |
SUPFAMi | SSF48371, SSF48371, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | HTR5B_HUMAN | |
Accessioni | Q9P2D3Primary (citable) accession number: Q9P2D3 Secondary accession number(s): B5MDU8, Q7Z3B2, Q9NVL7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 4, 2007 |
Last sequence update: | December 4, 2007 | |
Last modified: | May 25, 2022 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families