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Protein

Serine/threonine-protein kinase pim-2

Gene

PIM2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity. The stabilization of MYC exerted by PIM2 might explain partly the strong synergism between these 2 oncogenes in tumorigenesis. Regulates cap-dependent protein translation in a mammalian target of rapamycin complex 1 (mTORC1)-independent manner and in parallel to the PI3K-Akt pathway. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappa-B kinase/NF-kappa-B cascade; this process requires phosphorylation of MAP3K8/COT. Promotes growth factor-independent proliferation by phosphorylation of cell cycle factors such as CDKN1A and CDKN1B. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61ATPPROSITE-ProRule annotation1
Active sitei163Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 46ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processApoptosis, Cell cycle
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ9P1W9
SIGNORiQ9P1W9

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase pim-2 (EC:2.7.11.1)
Alternative name(s):
Pim-2h
Gene namesi
Name:PIM2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000102096.9
HGNCiHGNC:8987 PIM2
MIMi300295 gene
neXtProtiNX_Q9P1W9

Subcellular locationi

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

DisGeNETi11040
OpenTargetsiENSG00000102096
PharmGKBiPA33319

Chemistry databases

ChEMBLiCHEMBL4523
GuidetoPHARMACOLOGYi2159

Polymorphism and mutation databases

BioMutaiPIM2
DMDMi20139243

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000865321 – 311Serine/threonine-protein kinase pim-2Add BLAST311

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9P1W9
MaxQBiQ9P1W9
PaxDbiQ9P1W9
PeptideAtlasiQ9P1W9
PRIDEiQ9P1W9
ProteomicsDBi83676

PTM databases

iPTMnetiQ9P1W9
PhosphoSitePlusiQ9P1W9

Expressioni

Tissue specificityi

Highly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells. Weakly expressed in normal liver, but highly expressed in hepatocellular carcinoma tissues.1 Publication

Inductioni

Down-regulated in response to enterovirus 71 (EV71) infection.1 Publication

Gene expression databases

BgeeiENSG00000102096 Expressed in 147 organ(s), highest expression level in lymph node
CleanExiHS_PIM2
ExpressionAtlasiQ9P1W9 baseline and differential
GenevisibleiQ9P1W9 HS

Organism-specific databases

HPAiHPA000285

Interactioni

Subunit structurei

Interacts with MYC.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ZNF821O755413EBI-720425,EBI-740865

Protein-protein interaction databases

BioGridi116228, 17 interactors
IntActiQ9P1W9, 19 interactors
MINTiQ9P1W9
STRINGi9606.ENSP00000365692

Chemistry databases

BindingDBiQ9P1W9

Structurei

Secondary structure

1311
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9P1W9
SMRiQ9P1W9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9P1W9

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 286Protein kinasePROSITE-ProRule annotationAdd BLAST255

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0583 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00760000119045
HOGENOMiHOG000231357
HOVERGENiHBG106681
InParanoidiQ9P1W9
KOiK08806
OMAiQQWARLP
OrthoDBiEOG091G0IMW
PhylomeDBiQ9P1W9
TreeFamiTF320810

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR017348 PIM1/2/3
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PIRSFiPIRSF037993 STPK_Pim-1, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9P1W9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLTKPLQGPP APPGTPTPPP GGKDREAFEA EYRLGPLLGK GGFGTVFAGH
60 70 80 90 100
RLTDRLQVAI KVIPRNRVLG WSPLSDSVTC PLEVALLWKV GAGGGHPGVI
110 120 130 140 150
RLLDWFETQE GFMLVLERPL PAQDLFDYIT EKGPLGEGPS RCFFGQVVAA
160 170 180 190 200
IQHCHSRGVV HRDIKDENIL IDLRRGCAKL IDFGSGALLH DEPYTDFDGT
210 220 230 240 250
RVYSPPEWIS RHQYHALPAT VWSLGILLYD MVCGDIPFER DQEILEAELH
260 270 280 290 300
FPAHVSPDCC ALIRRCLAPK PSSRPSLEEI LLDPWMQTPA EDVPLNPSKG
310
GPAPLAWSLL P
Length:311
Mass (Da):34,190
Last modified:October 1, 2000 - v1
Checksum:i160E56C368C67A69
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9J8V9C9J8V9_HUMAN
Serine/threonine-protein kinase pim...
PIM2
145Annotation score:

Sequence cautioni

The sequence AAC78506 differs from that shown. Reason: Frameshift at position 293.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66N → Y in BAF83620 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_041008138G → D1 PublicationCorresponds to variant dbSNP:rs35044770Ensembl.1
Natural variantiVAR_041009280I → V1 PublicationCorresponds to variant dbSNP:rs35208542Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77735 mRNA Translation: AAC78506.1 Frameshift.
AK290931 mRNA Translation: BAF83620.1
AB042425 Genomic DNA Translation: BAA95613.1
AF207550 Genomic DNA No translation available.
BC018111 mRNA Translation: AAH18111.1
CCDSiCCDS14312.1
RefSeqiNP_006866.2, NM_006875.3
UniGeneiHs.727148

Genome annotation databases

EnsembliENST00000376509; ENSP00000365692; ENSG00000102096
GeneIDi11040
KEGGihsa:11040
UCSCiuc004dls.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77735 mRNA Translation: AAC78506.1 Frameshift.
AK290931 mRNA Translation: BAF83620.1
AB042425 Genomic DNA Translation: BAA95613.1
AF207550 Genomic DNA No translation available.
BC018111 mRNA Translation: AAH18111.1
CCDSiCCDS14312.1
RefSeqiNP_006866.2, NM_006875.3
UniGeneiHs.727148

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IWIX-ray2.80A/B1-311[»]
4X7QX-ray2.33A/B1-311[»]
ProteinModelPortaliQ9P1W9
SMRiQ9P1W9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116228, 17 interactors
IntActiQ9P1W9, 19 interactors
MINTiQ9P1W9
STRINGi9606.ENSP00000365692

Chemistry databases

BindingDBiQ9P1W9
ChEMBLiCHEMBL4523
GuidetoPHARMACOLOGYi2159

PTM databases

iPTMnetiQ9P1W9
PhosphoSitePlusiQ9P1W9

Polymorphism and mutation databases

BioMutaiPIM2
DMDMi20139243

Proteomic databases

EPDiQ9P1W9
MaxQBiQ9P1W9
PaxDbiQ9P1W9
PeptideAtlasiQ9P1W9
PRIDEiQ9P1W9
ProteomicsDBi83676

Protocols and materials databases

DNASUi11040
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000376509; ENSP00000365692; ENSG00000102096
GeneIDi11040
KEGGihsa:11040
UCSCiuc004dls.4 human

Organism-specific databases

CTDi11040
DisGeNETi11040
EuPathDBiHostDB:ENSG00000102096.9
GeneCardsiPIM2
HGNCiHGNC:8987 PIM2
HPAiHPA000285
MIMi300295 gene
neXtProtiNX_Q9P1W9
OpenTargetsiENSG00000102096
PharmGKBiPA33319
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0583 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00760000119045
HOGENOMiHOG000231357
HOVERGENiHBG106681
InParanoidiQ9P1W9
KOiK08806
OMAiQQWARLP
OrthoDBiEOG091G0IMW
PhylomeDBiQ9P1W9
TreeFamiTF320810

Enzyme and pathway databases

SignaLinkiQ9P1W9
SIGNORiQ9P1W9

Miscellaneous databases

ChiTaRSiPIM2 human
EvolutionaryTraceiQ9P1W9
GeneWikiiPIM2_(gene)
GenomeRNAii11040
PROiPR:Q9P1W9
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000102096 Expressed in 147 organ(s), highest expression level in lymph node
CleanExiHS_PIM2
ExpressionAtlasiQ9P1W9 baseline and differential
GenevisibleiQ9P1W9 HS

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR017348 PIM1/2/3
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PIRSFiPIRSF037993 STPK_Pim-1, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPIM2_HUMAN
AccessioniPrimary (citable) accession number: Q9P1W9
Secondary accession number(s): A8K4G6, Q99739
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: October 1, 2000
Last modified: November 7, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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Main funding by: National Institutes of Health

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