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Protein

MyoD family inhibitor domain-containing protein

Gene

MDFIC

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator or repressor. Inhibits the transcriptional activation of Zic family proteins ZIC1, ZIC2 and ZIC3. Retains nuclear Zic proteins ZIC1, ZIC2 and ZIC3 in the cytoplasm. Modulates the expression from both cellular and viral promoters. Down-regulates Tat-dependent transcription of the human immunodeficiency virus type 1 (HIV-1) LTR by interacting with HIV-1 Tat and Rev and impairing their nuclear import, probably by rendering the NLS domains inaccessible to importin-beta. Also stimulates activation of human T-cell leukemia virus type I (HTLV-I) LTR. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways.5 Publications

GO - Molecular functioni

  • cyclin binding Source: UniProtKB
  • Tat protein binding Source: UniProtKB
  • transcription factor binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionActivator, Repressor
Biological processHost-virus interaction, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
MyoD family inhibitor domain-containing protein
Alternative name(s):
I-mfa domain-containing protein
Short name:
hIC
Gene namesi
Name:MDFICImported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000135272.9
HGNCiHGNC:28870 MDFIC
MIMi614511 gene
neXtProtiNX_Q9P1T7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi29969
OpenTargetsiENSG00000135272
PharmGKBiPA142671474

Polymorphism and mutation databases

BioMutaiMDFIC
DMDMi257051035

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002802221 – 246MyoD family inhibitor domain-containing proteinAdd BLAST246

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei128PhosphoserineCombined sources1
Modified residuei140PhosphoserineCombined sources1
Modified residuei143PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9P1T7
PaxDbiQ9P1T7
PeptideAtlasiQ9P1T7
PRIDEiQ9P1T7
ProteomicsDBi83663
83664 [Q9P1T7-1]
TopDownProteomicsiQ9P1T7-1 [Q9P1T7-1]

PTM databases

iPTMnetiQ9P1T7
PhosphoSitePlusiQ9P1T7
SwissPalmiQ9P1T7

Expressioni

Tissue specificityi

Expressed in lymphoid organs (spleen, thymus, peripheral blood leukocytes) as well as prostate, uterus and small intestine.1 Publication

Gene expression databases

BgeeiENSG00000135272
CleanExiHS_MDFIC
ExpressionAtlasiQ9P1T7 baseline and differential
GenevisibleiQ9P1T7 HS

Organism-specific databases

HPAiHPA030716

Interactioni

Subunit structurei

Interacts with HAND1; leading to sequester HAND1 into the nucleolus and prevent its activity. Interacts with ZIC2 (By similarity). The C-terminus interacts with AXIN1, the histidine-rich region of CCNT1/cyclin-T and weakly with LEF1 (PubMed:12192039). Interacts with CCNT2 (PubMed:17289077).By similarity2 Publications
(Microbial infection) Interacts (via C-terminus) with HIV-1 Tat and Rev.3 Publications

GO - Molecular functioni

  • cyclin binding Source: UniProtKB
  • Tat protein binding Source: UniProtKB
  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi119002, 7 interactors
IntActiQ9P1T7, 10 interactors
STRINGi9606.ENSP00000257724

Structurei

3D structure databases

ProteinModelPortaliQ9P1T7
SMRiQ9P1T7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 246MDFIAdd BLAST173

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi166 – 243Cys-richAdd BLAST78

Domaini

The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation (By similarity). The cysteine-rich C-terminus is involved in its granular distribution in the cytoplasm.By similarity1 Publication

Sequence similaritiesi

Belongs to the MDFI family.Sequence analysis

Phylogenomic databases

eggNOGiENOG410IKRS Eukaryota
ENOG4111J6Q LUCA
GeneTreeiENSGT00390000010600
HOGENOMiHOG000001577
HOVERGENiHBG068212
InParanoidiQ9P1T7
PhylomeDBiQ9P1T7

Family and domain databases

InterProiView protein in InterPro
IPR026134 MDFI/MDFIC
PANTHERiPTHR15304 PTHR15304, 1 hit
PfamiView protein in Pfam
PF15316 MDFI, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 22 Publications (identifier: Q9P1T7-2) [UniParc]FASTAAdd to basket
Also known as: p321 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGAGEALAP GPVGPQRVAE AGGGQLGSTA QGKCDKDNTE KDITQATNSH
60 70 80 90 100
FTHGEMQDQS IWGNPSDGEL IRTQPQRLPQ LQTSAQVPSG EEIGKIKNGH
110 120 130 140 150
TGLSNGNGIH HGAKHGSADN RKLSAPVSQK MHRKIQSSLS VNSDISKKSK
160 170 180 190 200
VNAVFSQKTG SSPEDCCVHC ILACLFCEFL TLCNIVLGQA SCGICTSEAC
210 220 230 240
CCCCGDEMGD DCNCPCDMDC GIMDACCESS DCLEICMECC GICFPS
Note: Major isoform.
Length:246
Mass (Da):25,788
Last modified:September 1, 2009 - v2
Checksum:iB8DAAE9ADF5772A0
GO
Isoform 11 Publication (identifier: Q9P1T7-1) [UniParc]FASTAAdd to basket
Also known as: p401 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MRGVRAATAA...RGAGSAERPM

Note: Minor isoform. Initiates from a GTG codon. Contains a Nucleolar localization signal at positions 45-63.
Show »
Length:355
Mass (Da):37,042
Checksum:i47D3E541AB35D23E
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0379701M → MRGVRAATAAAVAATAASGL SRREAGGRAGAAAAVVRPPG RKCGRCRRLANFPGRKRRRR RRKGLGATTGGCGEAVSSLH PAPHSPSSVRPAGRRARRQR RGAGSAERPM in isoform 1. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF054589 mRNA Translation: AAF36998.1
AF054589 mRNA Translation: AAF36999.1
AY196485 mRNA Translation: AAP33842.1
CCDSiCCDS34737.1 [Q9P1T7-1]
CCDS55155.1 [Q9P1T7-2]
RefSeqiNP_001159817.1, NM_001166345.1 [Q9P1T7-2]
NP_951038.1, NM_199072.4 [Q9P1T7-1]
UniGeneiHs.741413

Genome annotation databases

EnsembliENST00000393486; ENSP00000377126; ENSG00000135272 [Q9P1T7-2]
ENST00000614186; ENSP00000484656; ENSG00000135272 [Q9P1T7-1]
GeneIDi29969
KEGGihsa:29969
UCSCiuc064hfm.1 human [Q9P1T7-2]

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiMDFIC_HUMAN
AccessioniPrimary (citable) accession number: Q9P1T7
Secondary accession number(s): Q9P1T6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: September 1, 2009
Last modified: July 18, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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