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Protein

C-type lectin domain family 1 member B

Gene

CLEC1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

C-type lectin-like receptor that functions as a platelet receptor for the lymphatic endothelial marker, PDPN (PubMed:18215137). After ligand activation, signals via sequential activation of SRC and SYK tyrosine kinases leading to activation of PLCG2 (PubMed:18955485).2 Publications
(Microbial infection) Acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (SYK) and initiation of downstream tyrosine phosphorylation events and activation of PLCG2 (PubMed:16174766, PubMed:18955485).1 Publication1 Publication
(Microbial infection) Acts as an attachment factor for Human immunodeficiency virus type 1 (HIV-1) and facilitates its capture by platelets (PubMed:16940507).1 Publication

GO - Molecular functioni

  • carbohydrate binding Source: UniProtKB-KW
  • transmembrane signaling receptor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionReceptor
LigandLectin

Enzyme and pathway databases

ReactomeiR-HSA-114604 GPVI-mediated activation cascade

Names & Taxonomyi

Protein namesi
Recommended name:
C-type lectin domain family 1 member B
Alternative name(s):
C-type lectin-like receptor 2
Short name:
CLEC-2
Gene namesi
Name:CLEC1B
Synonyms:CLEC2
ORF Names:UNQ721/PRO1384
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000165682.14
HGNCiHGNC:24356 CLEC1B
MIMi606783 gene
neXtProtiNX_Q9P126

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33CytoplasmicSequence analysisAdd BLAST33
Transmembranei34 – 54Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini55 – 229ExtracellularSequence analysisAdd BLAST175

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi7Y → F: Loss of activation upon podoplanin or rhodocytin stimulation. 1 Publication1
Mutagenesisi150K → A: Substantial reduction in rhodocytin binding. 1 Publication1
Mutagenesisi171K → A: Significant reduction in rhodocytin binding. 1 Publication1
Mutagenesisi184E → A: Significant reduction in rhodocytin binding. 1 Publication1
Mutagenesisi187E → A: Significant reduction in rhodocytin binding. 1 Publication1
Mutagenesisi188D → A: Significant reduction in rhodocytin binding. 1 Publication1
Mutagenesisi190K → A: Significant reduction in rhodocytin binding. 1 Publication1
Mutagenesisi192N → A: Significant reduction in rhodocytin binding. 1 Publication1

Organism-specific databases

DisGeNETi51266
OpenTargetsiENSG00000165682
PharmGKBiPA142672098

Polymorphism and mutation databases

BioMutaiCLEC1B
DMDMi134035066

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002800431 – 229C-type lectin domain family 1 member BAdd BLAST229

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7Phosphotyrosine1 Publication1
Glycosylationi68N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi102 ↔ 113PROSITE-ProRule annotation1 Publication
Glycosylationi120N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi130 ↔ 216PROSITE-ProRule annotation1 Publication
Glycosylationi134N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi195 ↔ 208PROSITE-ProRule annotation1 Publication

Post-translational modificationi

Glycosylated.2 Publications
Phosphorylated on tyrosine residue in response to rhodocytin binding.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9P126
PeptideAtlasiQ9P126
PRIDEiQ9P126
ProteomicsDBi83635
83636 [Q9P126-2]

PTM databases

iPTMnetiQ9P126
PhosphoSitePlusiQ9P126

Expressioni

Tissue specificityi

Expressed preferentially in the liver. Also expressed in immune cells of myeloid origin and on the surface of platelets.3 Publications

Gene expression databases

BgeeiENSG00000165682 Expressed in 69 organ(s), highest expression level in liver
CleanExiHS_CLEC1B
ExpressionAtlasiQ9P126 baseline and differential
GenevisibleiQ9P126 HS

Interactioni

Subunit structurei

Homodimer. Interacts (via cytoplasmic domain) with RACK1; promotes CLEC1B ubiquitination and proteasome-mediated degradation. Interacts (dimer) with SYK (via SH2 domains). Interacts with PDPN; the interaction is independent of CLEC1B glycosylation and activates CLEC1B (PubMed:18215137).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PDPNQ86YL72EBI-16130833,EBI-723160

Protein-protein interaction databases

BioGridi119420, 3 interactors
DIPiDIP-61332N
IntActiQ9P126, 4 interactors
STRINGi9606.ENSP00000298527

Structurei

Secondary structure

1229
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9P126
SMRiQ9P126
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9P126

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini109 – 217C-type lectinPROSITE-ProRule annotationAdd BLAST109

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi7 – 10ITAM1 Publication4

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4297 Eukaryota
ENOG410XPJ1 LUCA
GeneTreeiENSGT00700000104266
HOGENOMiHOG000111777
HOVERGENiHBG094936
InParanoidiQ9P126
KOiK10070
OMAiEVWKWED
OrthoDBiEOG091G0OAQ
PhylomeDBiQ9P126
TreeFamiTF336674

Family and domain databases

CDDicd03593 CLECT_NK_receptors_like, 1 hit
Gene3Di3.10.100.10, 1 hit
InterProiView protein in InterPro
IPR001304 C-type_lectin-like
IPR016186 C-type_lectin-like/link_sf
IPR016187 CTDL_fold
IPR033992 NKR-like_CTLD
PfamiView protein in Pfam
PF00059 Lectin_C, 1 hit
SMARTiView protein in SMART
SM00034 CLECT, 1 hit
SUPFAMiSSF56436 SSF56436, 1 hit
PROSITEiView protein in PROSITE
PS50041 C_TYPE_LECTIN_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9P126-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MQDEDGYITL NIKTRKPALI SVGSASSSWW RVMALILLIL CVGMVVGLVA
60 70 80 90 100
LGIWSVMQRN YLQGENENRT GTLQQLAKRF CQYVVKQSEL KGTFKGHKCS
110 120 130 140 150
PCDTNWRYYG DSCYGFFRHN LTWEESKQYC TDMNATLLKI DNRNIVEYIK
160 170 180 190 200
ARTHLIRWVG LSRQKSNEVW KWEDGSVISE NMFEFLEDGK GNMNCAYFHN
210 220
GKMHPTFCEN KHYLMCERKA GMTKVDQLP
Length:229
Mass (Da):26,596
Last modified:March 6, 2007 - v2
Checksum:iB39BF202AAE6DDD9
GO
Isoform 2 (identifier: Q9P126-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     22-55: VGSASSSWWRVMALILLILCVGMVVGLVALGIWS → A

Show »
Length:196
Mass (Da):23,096
Checksum:i0BFA3D427640C3F5
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H7BYT7H7BYT7_HUMAN
C-type lectin domain family 1 membe...
CLEC1B
96Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41C → Y in AAF36777 (PubMed:10671229).Curated1
Sequence conflicti41C → Y (PubMed:16940507).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03104720I → V4 PublicationsCorresponds to variant dbSNP:rs612593Ensembl.1
Natural variantiVAR_03104824S → P1 PublicationCorresponds to variant dbSNP:rs2273986Ensembl.1
Natural variantiVAR_03104928S → F. Corresponds to variant dbSNP:rs2273987Ensembl.1
Natural variantiVAR_03105064G → D4 PublicationsCorresponds to variant dbSNP:rs583903Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02351522 – 55VGSAS…LGIWS → A in isoform 2. 1 PublicationAdd BLAST34

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124841 mRNA Translation: AAF36777.1
AY358599 mRNA Translation: AAQ88962.1
AC091814 Genomic DNA No translation available.
BC029554 mRNA Translation: AAH29554.1
CCDSiCCDS41751.1 [Q9P126-2]
CCDS41752.1 [Q9P126-1]
RefSeqiNP_001092901.1, NM_001099431.1 [Q9P126-2]
NP_057593.3, NM_016509.3 [Q9P126-1]
XP_005253439.1, XM_005253382.4 [Q9P126-1]
XP_011518987.1, XM_011520685.2 [Q9P126-2]
XP_016874884.1, XM_017019395.1 [Q9P126-1]
UniGeneiHs.409794

Genome annotation databases

EnsembliENST00000298527; ENSP00000298527; ENSG00000165682 [Q9P126-1]
ENST00000348658; ENSP00000327169; ENSG00000165682 [Q9P126-2]
ENST00000428126; ENSP00000406338; ENSG00000165682 [Q9P126-2]
GeneIDi51266
KEGGihsa:51266
UCSCiuc001qwu.5 human [Q9P126-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

CLEC-2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124841 mRNA Translation: AAF36777.1
AY358599 mRNA Translation: AAQ88962.1
AC091814 Genomic DNA No translation available.
BC029554 mRNA Translation: AAH29554.1
CCDSiCCDS41751.1 [Q9P126-2]
CCDS41752.1 [Q9P126-1]
RefSeqiNP_001092901.1, NM_001099431.1 [Q9P126-2]
NP_057593.3, NM_016509.3 [Q9P126-1]
XP_005253439.1, XM_005253382.4 [Q9P126-1]
XP_011518987.1, XM_011520685.2 [Q9P126-2]
XP_016874884.1, XM_017019395.1 [Q9P126-1]
UniGeneiHs.409794

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C6UX-ray1.60A100-221[»]
3WSRX-ray1.91A/B96-221[»]
3WWKX-ray2.98C/F/I/L96-221[»]
ProteinModelPortaliQ9P126
SMRiQ9P126
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119420, 3 interactors
DIPiDIP-61332N
IntActiQ9P126, 4 interactors
STRINGi9606.ENSP00000298527

PTM databases

iPTMnetiQ9P126
PhosphoSitePlusiQ9P126

Polymorphism and mutation databases

BioMutaiCLEC1B
DMDMi134035066

Proteomic databases

PaxDbiQ9P126
PeptideAtlasiQ9P126
PRIDEiQ9P126
ProteomicsDBi83635
83636 [Q9P126-2]

Protocols and materials databases

DNASUi51266
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000298527; ENSP00000298527; ENSG00000165682 [Q9P126-1]
ENST00000348658; ENSP00000327169; ENSG00000165682 [Q9P126-2]
ENST00000428126; ENSP00000406338; ENSG00000165682 [Q9P126-2]
GeneIDi51266
KEGGihsa:51266
UCSCiuc001qwu.5 human [Q9P126-1]

Organism-specific databases

CTDi51266
DisGeNETi51266
EuPathDBiHostDB:ENSG00000165682.14
GeneCardsiCLEC1B
HGNCiHGNC:24356 CLEC1B
MIMi606783 gene
neXtProtiNX_Q9P126
OpenTargetsiENSG00000165682
PharmGKBiPA142672098
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4297 Eukaryota
ENOG410XPJ1 LUCA
GeneTreeiENSGT00700000104266
HOGENOMiHOG000111777
HOVERGENiHBG094936
InParanoidiQ9P126
KOiK10070
OMAiEVWKWED
OrthoDBiEOG091G0OAQ
PhylomeDBiQ9P126
TreeFamiTF336674

Enzyme and pathway databases

ReactomeiR-HSA-114604 GPVI-mediated activation cascade

Miscellaneous databases

ChiTaRSiCLEC1B human
EvolutionaryTraceiQ9P126
GeneWikiiCLEC1B
GenomeRNAii51266
PROiPR:Q9P126
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000165682 Expressed in 69 organ(s), highest expression level in liver
CleanExiHS_CLEC1B
ExpressionAtlasiQ9P126 baseline and differential
GenevisibleiQ9P126 HS

Family and domain databases

CDDicd03593 CLECT_NK_receptors_like, 1 hit
Gene3Di3.10.100.10, 1 hit
InterProiView protein in InterPro
IPR001304 C-type_lectin-like
IPR016186 C-type_lectin-like/link_sf
IPR016187 CTDL_fold
IPR033992 NKR-like_CTLD
PfamiView protein in Pfam
PF00059 Lectin_C, 1 hit
SMARTiView protein in SMART
SM00034 CLECT, 1 hit
SUPFAMiSSF56436 SSF56436, 1 hit
PROSITEiView protein in PROSITE
PS50041 C_TYPE_LECTIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCLC1B_HUMAN
AccessioniPrimary (citable) accession number: Q9P126
Secondary accession number(s): Q6UWX7, Q8NHR6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: March 6, 2007
Last modified: September 12, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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