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Protein

Sentrin-specific protease 1

Gene

SENP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protease that catalyzes two essential functions in the SUMO pathway (PubMed:10652325, PubMed:15199155, PubMed:16253240, PubMed:16553580, PubMed:21829689, PubMed:21965678, PubMed:23160374, PubMed:24943844, PubMed:25406032, PubMed:29506078). The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein. Deconjugates SUMO1 from HIPK2 (PubMed:16253240). Deconjugates SUMO1 from HDAC1 and BHLHE40/DEC1, which decreases its transcriptional repression activity (PubMed:21829689). Deconjugates SUMO1 from CLOCK, which decreases its transcriptional activation activity (PubMed:23160374). Deconjugates SUMO2 from MTA1 (PubMed:21965678). Deconjugates SUMO1 from METTL3 (PubMed:29506078). Desumoylates CCAR2 which decreases its interaction with SIRT1 (PubMed:25406032). Deconjugates SUMO1 from GPS2 (PubMed:24943844).10 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei5331
Active sitei5501
Active sitei603Nucleophile1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

BRENDAi3.4.22.B70 2681
ReactomeiR-HSA-3065679 SUMO is proteolytically processed
SIGNORiQ9P0U3

Protein family/group databases

MEROPSiC48.002

Names & Taxonomyi

Protein namesi
Recommended name:
Sentrin-specific protease 1 (EC:3.4.22.-2 Publications)
Alternative name(s):
Sentrin/SUMO-specific protease SENP1
Gene namesi
Name:SENP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000079387.13
HGNCiHGNC:17927 SENP1
MIMi612157 gene
neXtProtiNX_Q9P0U3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi441D → A: No effect on SUMO2 processing and SUMO2 deconjugating activities. 1 Publication1
Mutagenesisi465W → A: Impairs SUMO2 processing and SUMO2 deconjugating activities. 1 Publication1
Mutagenesisi468D → A: Slightly impairs SUMO2 processing activity. No effect on SUMO2 deconjugating activity. 1 Publication1
Mutagenesisi496F → A: Impairs SUMO2 processing activity. No effect on SUMO2 deconjugating activity. 1 Publication1
Mutagenesisi511R → A: Impairs SUMO2 processing activity. No effect on SUMO2 deconjugating activity. 1 Publication1
Mutagenesisi512W → A: Impairs SUMO2 processing and SUMO2 deconjugating activities. 1 Publication1
Mutagenesisi529H → A: Impairs SUMO2 processing activity. No effect on SUMO2 deconjugating activity. 1 Publication1
Mutagenesisi532V → A: No effect on SUMO2 processing and SUMO2 deconjugating activities. 1 Publication1
Mutagenesisi533H → A: Abolishes SUMO2 processing and SUMO2 deconjugating activities. 1 Publication1
Mutagenesisi534W → A: Abolishes SUMO2 processing and SUMO2 deconjugating activities. 1 Publication1
Mutagenesisi550D → A: Abolishes SUMO2 processing and SUMO2 deconjugating activities. 1 Publication1
Mutagenesisi597Q → A: Abolishes SUMO2 processing and SUMO2 deconjugating activities. 1 Publication1
Mutagenesisi603C → A or S: Abolishes SUMO2 processing and SUMO2 deconjugating activities. 3 Publications1
Mutagenesisi603C → S: Exclusively nuclear. Loss of CCAR2 desumoylation. 4 Publications1

Organism-specific databases

DisGeNETi29843
OpenTargetsiENSG00000079387
PharmGKBiPA134947038

Chemistry databases

ChEMBLiCHEMBL1909484
GuidetoPHARMACOLOGYi2414

Polymorphism and mutation databases

BioMutaiSENP1
DMDMi215273882

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001017161 – 644Sentrin-specific protease 1Add BLAST644

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei57PhosphoserineCombined sources1
Modified residuei117PhosphoserineCombined sources1
Modified residuei132PhosphoserineCombined sources1
Modified residuei157PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9P0U3
MaxQBiQ9P0U3
PaxDbiQ9P0U3
PeptideAtlasiQ9P0U3
PRIDEiQ9P0U3
ProteomicsDBi83598
83599 [Q9P0U3-2]

PTM databases

iPTMnetiQ9P0U3
PhosphoSitePlusiQ9P0U3

Expressioni

Tissue specificityi

Highly expressed in testis. Expressed at lower levels in thymus, pancreas, spleen, liver, ovary and small intestine.1 Publication

Gene expression databases

BgeeiENSG00000079387 Expressed in 172 organ(s), highest expression level in female gonad
CleanExiHS_SENP1
ExpressionAtlasiQ9P0U3 baseline and differential
GenevisibleiQ9P0U3 HS

Organism-specific databases

HPAiHPA011765
HPA046491

Interactioni

Subunit structurei

Interacts with MTA1. Interacts with CCAR2 (via N-terminus).5 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi118930, 58 interactors
DIPiDIP-29252N
IntActiQ9P0U3, 18 interactors
MINTiQ9P0U3
STRINGi9606.ENSP00000394791

Chemistry databases

BindingDBiQ9P0U3

Structurei

Secondary structure

1644
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9P0U3
SMRiQ9P0U3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9P0U3

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 200Interaction with CCAR21 PublicationAdd BLAST200
Regioni450 – 613ProteaseAdd BLAST164

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi171 – 177Nuclear localization signal1 Publication7
Motifi574 – 577Nuclear localization signalSequence analysis4
Motifi628 – 634Nuclear localization signalSequence analysis7
Motifi635 – 644Nuclear export signalCurated10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi45 – 170Ser-richAdd BLAST126
Compositional biasi290 – 300His-richAdd BLAST11

Sequence similaritiesi

Belongs to the peptidase C48 family.Curated

Phylogenomic databases

eggNOGiKOG0778 Eukaryota
COG5160 LUCA
GeneTreeiENSGT00530000062941
HOGENOMiHOG000154286
HOVERGENiHBG057384
InParanoidiQ9P0U3
KOiK08592
OMAiLDRSYTC
OrthoDBiEOG091G092N
PhylomeDBiQ9P0U3
TreeFamiTF316289

Family and domain databases

InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR003653 Peptidase_C48_C
PfamiView protein in Pfam
PF02902 Peptidase_C48, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS50600 ULP_PROTEASE, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9P0U3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDDIADRMRM DAGEVTLVNH NSVFKTHLLP QTGFPEDQLS LSDQQILSSR
60 70 80 90 100
QGHLDRSFTC STRSAAYNPS YYSDNPSSDS FLGSGDLRTF GQSANGQWRN
110 120 130 140 150
STPSSSSSLQ KSRNSRSLYL ETRKTSSGLS NSFAGKSNHH CHVSAYEKSF
160 170 180 190 200
PIKPVPSPSW SGSCRRSLLS PKKTQRRHVS TAEETVQEEE REIYRQLLQM
210 220 230 240 250
VTGKQFTIAK PTTHFPLHLS RCLSSSKNTL KDSLFKNGNS CASQIIGSDT
260 270 280 290 300
SSSGSASILT NQEQLSHSVY SLSSYTPDVA FGSKDSGTLH HPHHHHSVPH
310 320 330 340 350
QPDNLAASNT QSEGSDSVIL LKVKDSQTPT PSSTFFQAEL WIKELTSVYD
360 370 380 390 400
SRARERLRQI EEQKALALQL QNQRLQEREH SVHDSVELHL RVPLEKEIPV
410 420 430 440 450
TVVQETQKKG HKLTDSEDEF PEITEEMEKE IKNVFRNGNQ DEVLSEAFRL
460 470 480 490 500
TITRKDIQTL NHLNWLNDEI INFYMNMLME RSKEKGLPSV HAFNTFFFTK
510 520 530 540 550
LKTAGYQAVK RWTKKVDVFS VDILLVPIHL GVHWCLAVVD FRKKNITYYD
560 570 580 590 600
SMGGINNEAC RILLQYLKQE SIDKKRKEFD TNGWQLFSKK SQEIPQQMNG
610 620 630 640
SDCGMFACKY ADCITKDRPI NFTQQHMPYF RKRMVWEILH RKLL
Length:644
Mass (Da):73,481
Last modified:November 25, 2008 - v2
Checksum:i941951A8B341EC64
GO
Isoform 2 (identifier: Q9P0U3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     593-593: Missing.

Note: No experimental confirmation available.
Show »
Length:643
Mass (Da):73,351
Checksum:i1556864F1BDEE337
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8W0I8F8W0I8_HUMAN
Sentrin-specific protease 1
SENP1
130Annotation score:
F8VRZ8F8VRZ8_HUMAN
Sentrin-specific protease 1
SENP1
142Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_029648193I → V1 PublicationCorresponds to variant dbSNP:rs17854369Ensembl.1
Natural variantiVAR_047547280A → T. Corresponds to variant dbSNP:rs35130318Ensembl.1
Natural variantiVAR_029649350D → G1 PublicationCorresponds to variant dbSNP:rs17854368Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_035777593Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149770 mRNA Translation: AAF31171.1
AK292060 mRNA Translation: BAF84749.1
AC074029 Genomic DNA No translation available.
BC045639 mRNA Translation: AAH45639.2
CCDSiCCDS44868.2 [Q9P0U3-1]
RefSeqiNP_001254523.1, NM_001267594.1 [Q9P0U3-1]
NP_001254524.1, NM_001267595.1 [Q9P0U3-1]
UniGeneiHs.371957

Genome annotation databases

EnsembliENST00000448372; ENSP00000394791; ENSG00000079387 [Q9P0U3-1]
ENST00000549518; ENSP00000447328; ENSG00000079387 [Q9P0U3-1]
ENST00000549595; ENSP00000450076; ENSG00000079387 [Q9P0U3-2]
GeneIDi29843
KEGGihsa:29843
UCSCiuc001rqx.5 human [Q9P0U3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149770 mRNA Translation: AAF31171.1
AK292060 mRNA Translation: BAF84749.1
AC074029 Genomic DNA No translation available.
BC045639 mRNA Translation: AAH45639.2
CCDSiCCDS44868.2 [Q9P0U3-1]
RefSeqiNP_001254523.1, NM_001267594.1 [Q9P0U3-1]
NP_001254524.1, NM_001267595.1 [Q9P0U3-1]
UniGeneiHs.371957

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CKGX-ray2.45A/B419-644[»]
2CKHX-ray3.20A419-644[»]
2G4DX-ray2.80A/C440-644[»]
2IY0X-ray2.77A419-644[»]
2IY1X-ray2.46A/C419-644[»]
2IYCX-ray2.45A/B419-644[»]
2IYDX-ray3.20A419-644[»]
2XPHX-ray2.40A/B415-644[»]
2XREX-ray2.45A/B415-644[»]
ProteinModelPortaliQ9P0U3
SMRiQ9P0U3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118930, 58 interactors
DIPiDIP-29252N
IntActiQ9P0U3, 18 interactors
MINTiQ9P0U3
STRINGi9606.ENSP00000394791

Chemistry databases

BindingDBiQ9P0U3
ChEMBLiCHEMBL1909484
GuidetoPHARMACOLOGYi2414

Protein family/group databases

MEROPSiC48.002

PTM databases

iPTMnetiQ9P0U3
PhosphoSitePlusiQ9P0U3

Polymorphism and mutation databases

BioMutaiSENP1
DMDMi215273882

Proteomic databases

EPDiQ9P0U3
MaxQBiQ9P0U3
PaxDbiQ9P0U3
PeptideAtlasiQ9P0U3
PRIDEiQ9P0U3
ProteomicsDBi83598
83599 [Q9P0U3-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000448372; ENSP00000394791; ENSG00000079387 [Q9P0U3-1]
ENST00000549518; ENSP00000447328; ENSG00000079387 [Q9P0U3-1]
ENST00000549595; ENSP00000450076; ENSG00000079387 [Q9P0U3-2]
GeneIDi29843
KEGGihsa:29843
UCSCiuc001rqx.5 human [Q9P0U3-1]

Organism-specific databases

CTDi29843
DisGeNETi29843
EuPathDBiHostDB:ENSG00000079387.13
GeneCardsiSENP1
HGNCiHGNC:17927 SENP1
HPAiHPA011765
HPA046491
MIMi612157 gene
neXtProtiNX_Q9P0U3
OpenTargetsiENSG00000079387
PharmGKBiPA134947038
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0778 Eukaryota
COG5160 LUCA
GeneTreeiENSGT00530000062941
HOGENOMiHOG000154286
HOVERGENiHBG057384
InParanoidiQ9P0U3
KOiK08592
OMAiLDRSYTC
OrthoDBiEOG091G092N
PhylomeDBiQ9P0U3
TreeFamiTF316289

Enzyme and pathway databases

BRENDAi3.4.22.B70 2681
ReactomeiR-HSA-3065679 SUMO is proteolytically processed
SIGNORiQ9P0U3

Miscellaneous databases

ChiTaRSiSENP1 human
EvolutionaryTraceiQ9P0U3
GeneWikiiSENP1
GenomeRNAii29843
PROiPR:Q9P0U3
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000079387 Expressed in 172 organ(s), highest expression level in female gonad
CleanExiHS_SENP1
ExpressionAtlasiQ9P0U3 baseline and differential
GenevisibleiQ9P0U3 HS

Family and domain databases

InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR003653 Peptidase_C48_C
PfamiView protein in Pfam
PF02902 Peptidase_C48, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS50600 ULP_PROTEASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSENP1_HUMAN
AccessioniPrimary (citable) accession number: Q9P0U3
Secondary accession number(s): A8K7P5, Q86XC8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: November 25, 2008
Last modified: November 7, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
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