UniProtKB - Q9P0T7 (TMEM9_HUMAN)
Protein
Proton-transporting V-type ATPase complex assembly regulator TMEM9
Gene
TMEM9
Organism
Homo sapiens (Human)
Status
Functioni
Transmembrane protein that binds to and facilitates the assembly of lysosomal proton-transporting V-type ATPase (v-ATPase), resulting in enhanced lysosomal acidification and trafficking (PubMed:30374053). By bringing the v-ATPase accessory protein ATP6AP2 and the v-ATPase subunit ATP6V0D1 together, allows v-ATPase complex formation and activation (PubMed:30374053). TMEM9-controlled vesicular acidification induces hyperactivation of Wnt/beta-catenin signaling, involved in development, tissue homeostasis and tissue regeneration, through lysosomal degradation of adenomatous polyposis coli/APC (PubMed:30374053, PubMed:32380568). In the liver, involved in hepatic regeneration (PubMed:32380568).2 Publications
GO - Biological processi
- endosomal lumen acidification Source: UniProtKB
- lysosomal lumen acidification Source: UniProtKB
- positive regulation of canonical Wnt signaling pathway Source: UniProtKB
- protein transport Source: UniProtKB-KW
- proton-transporting V-type ATPase complex assembly Source: UniProtKB
- regulation of protein catabolic process Source: UniProtKB
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q9P0T7 |
Protein family/group databases
TCDBi | 8.A.123.1.1, the cytokine expression and secretion mediator and v-type atpase regulator, tmem9 (tmem9) family |
Names & Taxonomyi
Protein namesi | Recommended name: Proton-transporting V-type ATPase complex assembly regulator TMEM91 PublicationShort name: v-ATPase assembly regulator TMEM91 Publication Alternative name(s): Dermal papilla-derived protein 41 Publication Transmembrane protein 91 Publication Short name: Protein TMEM91 Publication |
Gene namesi | ORF Names:HSPC186, PSEC0012, UNQ631/PRO1248 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000116857.16 |
HGNCi | HGNC:18823, TMEM9 |
MIMi | 616877, gene |
neXtProti | NX_Q9P0T7 |
Subcellular locationi
Endosome
- Late endosome membrane 2 Publications; Single-pass type I membrane protein Sequence analysis
- multivesicular body membrane 1 Publication; Single-pass type I membrane protein Sequence analysis
Lysosome
- Lysosome membrane 1 Publication1 Publication; Single-pass type I membrane protein Sequence analysis
Endosome
- late endosome Source: UniProtKB
- multivesicular body membrane Source: UniProtKB
Lysosome
- lysosomal membrane Source: UniProtKB
- lysosome Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 21 – 89 | ExtracellularSequence analysisAdd BLAST | 69 | |
Transmembranei | 90 – 110 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 111 – 183 | CytoplasmicSequence analysisAdd BLAST | 73 |
Keywords - Cellular componenti
Endosome, Lysosome, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 252839 |
OpenTargetsi | ENSG00000116857 |
PharmGKBi | PA38696 |
Miscellaneous databases
Pharosi | Q9P0T7, Tbio |
Polymorphism and mutation databases
BioMutai | TMEM9 |
DMDMi | 20532090 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
ChainiPRO_0000034374 | 21 – 183 | Proton-transporting V-type ATPase complex assembly regulator TMEM9Add BLAST | 163 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 21 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 38 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 47 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 137 | PhosphoserineCombined sources | 1 | |
Modified residuei | 144 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
N-glycosylated.1 Publication
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
EPDi | Q9P0T7 |
jPOSTi | Q9P0T7 |
MassIVEi | Q9P0T7 |
MaxQBi | Q9P0T7 |
PaxDbi | Q9P0T7 |
PeptideAtlasi | Q9P0T7 |
PRIDEi | Q9P0T7 |
ProteomicsDBi | 83596 |
PTM databases
GlyConnecti | 1857, 1 N-Linked glycan (1 site) |
GlyGeni | Q9P0T7, 4 sites |
iPTMneti | Q9P0T7 |
PhosphoSitePlusi | Q9P0T7 |
Expressioni
Tissue specificityi
Highly expressed in adrenal gland, thyroid gland, testis, ovary and prostate (PubMed:12359240). Moderate expression in trachea, spinal cord, stomach, colon, small intestine and spleen (PubMed:12359240, PubMed:30374053). Low expression in bone marrow, lymph node, thymus and peripheral blood lymphocytes (PubMed:12359240). Expression is detected in hematopoietic cell lines including those of myeloid, erythroid, B- and T-cell origin (PubMed:12359240).2 Publications
Gene expression databases
Bgeei | ENSG00000116857, Expressed in hypothalamus and 217 other tissues |
ExpressionAtlasi | Q9P0T7, baseline and differential |
Genevisiblei | Q9P0T7, HS |
Organism-specific databases
HPAi | ENSG00000116857, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with the v-ATPase accessory protein ATP6AP2 and with the v-ATPase complex subunit ATP6V0D1; these interactions lead to the assembly of the v-ATPase complex.
1 PublicationBinary interactionsi
Hide detailsQ9P0T7
Protein-protein interaction databases
BioGRIDi | 128927, 117 interactors |
IntActi | Q9P0T7, 18 interactors |
STRINGi | 9606.ENSP00000356301 |
Miscellaneous databases
RNActi | Q9P0T7, protein |
Family & Domainsi
Domaini
The transmembrane domain (TMD) is essential for the interaction with ATP6AP2.1 Publication
Sequence similaritiesi
Belongs to the TMEM9 family.Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4007, Eukaryota |
GeneTreei | ENSGT00390000000819 |
HOGENOMi | CLU_093267_2_0_1 |
InParanoidi | Q9P0T7 |
OrthoDBi | 1380345at2759 |
PhylomeDBi | Q9P0T7 |
TreeFami | TF315146 |
Family and domain databases
InterProi | View protein in InterPro IPR008853, TMEM9 |
PANTHERi | PTHR13064, PTHR13064, 1 hit |
Pfami | View protein in Pfam PF05434, Tmemb_9, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
Q9P0T7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLLSLVAVV GCLLVPPAEA NKSSEDIRCK CICPPYRNIS GHIYNQNVSQ
60 70 80 90 100
KDCNCLHVVE PMPVPGHDVE AYCLLCECRY EERSTTTIKV IIVIYLSVVG
110 120 130 140 150
ALLLYMAFLM LVDPLIRKPD AYTEQLHNEE ENEDARSMAA AAASLGGPRA
160 170 180
NTVLERVEGA QQRWKLQVQE QRKTVFDRHK MLS
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB1ALM5 | B1ALM5_HUMAN | Proton-transporting V-type ATPase c... | TMEM9 | 186 | Annotation score: | ||
B1ALM7 | B1ALM7_HUMAN | Proton-transporting V-type ATPase c... | TMEM9 | 138 | Annotation score: | ||
B1ALM8 | B1ALM8_HUMAN | Proton-transporting V-type ATPase c... | TMEM9 | 151 | Annotation score: | ||
B1ALM4 | B1ALM4_HUMAN | Proton-transporting V-type ATPase c... | TMEM9 | 163 | Annotation score: | ||
F2Z389 | F2Z389_HUMAN | Proton-transporting V-type ATPase c... | TMEM9 | 98 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 22 | K → KVRE in BAB70820 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 53 | C → CCS in CAB66535 (PubMed:11230166).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY138587 mRNA Translation: AAN15925.1 AB013909 mRNA Translation: BAB87799.1 AF151020 mRNA Translation: AAF36106.1 AL136600 mRNA Translation: CAB66535.1 AY359012 mRNA Translation: AAQ89371.1 AK054883 mRNA Translation: BAB70820.1 AK075335 mRNA Translation: BAC11554.1 AL139159 Genomic DNA No translation available. CH471067 Genomic DNA Translation: EAW91338.1 BC001106 mRNA Translation: AAH01106.1 |
CCDSi | CCDS1408.1 |
RefSeqi | NP_001275493.1, NM_001288564.1 NP_001275494.1, NM_001288565.1 NP_001275495.1, NM_001288566.1 NP_001275496.1, NM_001288567.1 NP_001275497.1, NM_001288568.1 NP_001275498.1, NM_001288569.1 NP_001275499.1, NM_001288570.1 NP_001275500.1, NM_001288571.1 NP_057540.1, NM_016456.4 XP_011507687.1, XM_011509385.1 XP_011507688.1, XM_011509386.2 XP_011507689.1, XM_011509387.2 |
Genome annotation databases
Ensembli | ENST00000367330; ENSP00000356299; ENSG00000116857 ENST00000367333; ENSP00000356302; ENSG00000116857 ENST00000367334; ENSP00000356303; ENSG00000116857 ENST00000485839; ENSP00000476893; ENSG00000116857 |
GeneIDi | 252839 |
KEGGi | hsa:252839 |
UCSCi | uc001gvx.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY138587 mRNA Translation: AAN15925.1 AB013909 mRNA Translation: BAB87799.1 AF151020 mRNA Translation: AAF36106.1 AL136600 mRNA Translation: CAB66535.1 AY359012 mRNA Translation: AAQ89371.1 AK054883 mRNA Translation: BAB70820.1 AK075335 mRNA Translation: BAC11554.1 AL139159 Genomic DNA No translation available. CH471067 Genomic DNA Translation: EAW91338.1 BC001106 mRNA Translation: AAH01106.1 |
CCDSi | CCDS1408.1 |
RefSeqi | NP_001275493.1, NM_001288564.1 NP_001275494.1, NM_001288565.1 NP_001275495.1, NM_001288566.1 NP_001275496.1, NM_001288567.1 NP_001275497.1, NM_001288568.1 NP_001275498.1, NM_001288569.1 NP_001275499.1, NM_001288570.1 NP_001275500.1, NM_001288571.1 NP_057540.1, NM_016456.4 XP_011507687.1, XM_011509385.1 XP_011507688.1, XM_011509386.2 XP_011507689.1, XM_011509387.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 128927, 117 interactors |
IntActi | Q9P0T7, 18 interactors |
STRINGi | 9606.ENSP00000356301 |
Protein family/group databases
TCDBi | 8.A.123.1.1, the cytokine expression and secretion mediator and v-type atpase regulator, tmem9 (tmem9) family |
PTM databases
GlyConnecti | 1857, 1 N-Linked glycan (1 site) |
GlyGeni | Q9P0T7, 4 sites |
iPTMneti | Q9P0T7 |
PhosphoSitePlusi | Q9P0T7 |
Polymorphism and mutation databases
BioMutai | TMEM9 |
DMDMi | 20532090 |
Proteomic databases
EPDi | Q9P0T7 |
jPOSTi | Q9P0T7 |
MassIVEi | Q9P0T7 |
MaxQBi | Q9P0T7 |
PaxDbi | Q9P0T7 |
PeptideAtlasi | Q9P0T7 |
PRIDEi | Q9P0T7 |
ProteomicsDBi | 83596 |
Protocols and materials databases
Antibodypediai | 34497, 56 antibodies |
DNASUi | 252839 |
Genome annotation databases
Ensembli | ENST00000367330; ENSP00000356299; ENSG00000116857 ENST00000367333; ENSP00000356302; ENSG00000116857 ENST00000367334; ENSP00000356303; ENSG00000116857 ENST00000485839; ENSP00000476893; ENSG00000116857 |
GeneIDi | 252839 |
KEGGi | hsa:252839 |
UCSCi | uc001gvx.5, human |
Organism-specific databases
CTDi | 252839 |
DisGeNETi | 252839 |
EuPathDBi | HostDB:ENSG00000116857.16 |
GeneCardsi | TMEM9 |
HGNCi | HGNC:18823, TMEM9 |
HPAi | ENSG00000116857, Low tissue specificity |
MIMi | 616877, gene |
neXtProti | NX_Q9P0T7 |
OpenTargetsi | ENSG00000116857 |
PharmGKBi | PA38696 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4007, Eukaryota |
GeneTreei | ENSGT00390000000819 |
HOGENOMi | CLU_093267_2_0_1 |
InParanoidi | Q9P0T7 |
OrthoDBi | 1380345at2759 |
PhylomeDBi | Q9P0T7 |
TreeFami | TF315146 |
Enzyme and pathway databases
PathwayCommonsi | Q9P0T7 |
Miscellaneous databases
BioGRID-ORCSi | 252839, 4 hits in 851 CRISPR screens |
ChiTaRSi | TMEM9, human |
GeneWikii | TMEM9 |
GenomeRNAii | 252839 |
Pharosi | Q9P0T7, Tbio |
PROi | PR:Q9P0T7 |
RNActi | Q9P0T7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000116857, Expressed in hypothalamus and 217 other tissues |
ExpressionAtlasi | Q9P0T7, baseline and differential |
Genevisiblei | Q9P0T7, HS |
Family and domain databases
InterProi | View protein in InterPro IPR008853, TMEM9 |
PANTHERi | PTHR13064, PTHR13064, 1 hit |
Pfami | View protein in Pfam PF05434, Tmemb_9, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TMEM9_HUMAN | |
Accessioni | Q9P0T7Primary (citable) accession number: Q9P0T7 Secondary accession number(s): B1ALM6, Q96NQ9, Q9BQF5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2002 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM