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Protein

E3 ubiquitin-protein ligase MARCH2

Gene

MARCH2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May be involved in endosomal trafficking through interaction with STX6.3 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri56 – 116RING-CH-typePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • endocytosis Source: UniProtKB-KW
  • protein ubiquitination Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processEndocytosis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase MARCH2 (EC:2.3.2.27)
Alternative name(s):
Membrane-associated RING finger protein 2
Membrane-associated RING-CH protein II
Short name:
MARCH-II
RING finger protein 172
RING-type E3 ubiquitin transferase MARCH2Curated
Gene namesi
Name:MARCH2
Synonyms:RNF172
ORF Names:HSPC240
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000099785.10
HGNCiHGNC:28038 MARCH2
MIMi613332 gene
neXtProtiNX_Q9P0N8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei138 – 158HelicalSequence analysisAdd BLAST21
Transmembranei175 – 195HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Endosome, Lysosome, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000099785
PharmGKBiPA134993565

Polymorphism and mutation databases

BioMutaiMARCH2
DMDMi57012977

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000559251 – 246E3 ubiquitin-protein ligase MARCH2Add BLAST246

Proteomic databases

PaxDbiQ9P0N8
PeptideAtlasiQ9P0N8
PRIDEiQ9P0N8
ProteomicsDBi83585
83586 [Q9P0N8-2]

Expressioni

Tissue specificityi

Broadly expressed.1 Publication

Gene expression databases

BgeeiENSG00000099785
CleanExiHS_MARCH2
ExpressionAtlasiQ9P0N8 baseline and differential
GenevisibleiQ9P0N8 HS

Organism-specific databases

HPAiHPA014063

Interactioni

Subunit structurei

Interacts with STX6 (By similarity). Interacts with MARCH3.By similarity1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi119413, 13 interactors
IntActiQ9P0N8, 7 interactors
STRINGi9606.ENSP00000215555

Structurei

3D structure databases

ProteinModelPortaliQ9P0N8
SMRiQ9P0N8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-CH-type zinc finger domain is required for E3 ligase activity.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri56 – 116RING-CH-typePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1609 Eukaryota
COG5183 LUCA
GeneTreeiENSGT00920000148985
HOGENOMiHOG000293394
HOVERGENiHBG052411
InParanoidiQ9P0N8
KOiK10657
OMAiEGPQHSP
OrthoDBiEOG091G14LW
PhylomeDBiQ9P0N8
TreeFamiTF319557

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR033275 MARCH-like
IPR011016 Znf_RING-CH
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR23012 PTHR23012, 1 hit
PfamiView protein in Pfam
PF12906 RINGv, 1 hit
SMARTiView protein in SMART
SM00744 RINGv, 1 hit
PROSITEiView protein in PROSITE
PS51292 ZF_RING_CH, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9P0N8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTGDCCHLP GSLCDCSGSP AFSKVVEATG LGPPQYVAQV TSRDGRLLST
60 70 80 90 100
VIRALDTPSD GPFCRICHEG ANGECLLSPC GCTGTLGAVH KSCLEKWLSS
110 120 130 140 150
SNTSYCELCH TEFAVEKRPR PLTEWLKDPG PRTEKRTLCC DMVCFLFITP
160 170 180 190 200
LAAISGWLCL RGAQDHLRLH SQLEAVGLIA LTIALFTIYV LWTLVSFRYH
210 220 230 240
CQLYSEWRKT NQKVRLKIRE ADSPEGPQHS PLAAGLLKKV AEETPV
Length:246
Mass (Da):26,995
Last modified:October 1, 2000 - v1
Checksum:iFBD877D55211C929
GO
Isoform 2 (identifier: Q9P0N8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     125-194: Missing.

Note: No experimental confirmation available.
Show »
Length:176
Mass (Da):19,099
Checksum:i1AA18937FB6F7652
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03030354A → T1 PublicationCorresponds to variant dbSNP:rs1133893Ensembl.1
Natural variantiVAR_053638219R → P. Corresponds to variant dbSNP:rs34099346Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_041478125 – 194Missing in isoform 2. CuratedAdd BLAST70

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB197929 mRNA Translation: BAD89359.1
AF151074 mRNA Translation: AAF36160.1
AC136469 Genomic DNA No translation available.
BC032624 mRNA Translation: AAH32624.1
BC015910 mRNA Translation: AAH15910.1
BC111388 mRNA Translation: AAI11389.1
CCDSiCCDS12202.1 [Q9P0N8-1]
CCDS32894.1 [Q9P0N8-2]
RefSeqiNP_001005415.1, NM_001005415.1 [Q9P0N8-1]
NP_001005416.1, NM_001005416.1 [Q9P0N8-2]
NP_057580.3, NM_016496.4 [Q9P0N8-1]
XP_006722826.1, XM_006722763.3 [Q9P0N8-2]
XP_016882342.1, XM_017026853.1 [Q9P0N8-1]
UniGeneiHs.631861

Genome annotation databases

EnsembliENST00000215555; ENSP00000215555; ENSG00000099785 [Q9P0N8-1]
ENST00000381035; ENSP00000370423; ENSG00000099785 [Q9P0N8-2]
ENST00000602117; ENSP00000471536; ENSG00000099785 [Q9P0N8-1]
GeneIDi51257
KEGGihsa:51257
UCSCiuc002mjw.4 human [Q9P0N8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiMARH2_HUMAN
AccessioniPrimary (citable) accession number: Q9P0N8
Secondary accession number(s): A6NP10
, Q5H785, Q8N5A3, Q96B78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 1, 2000
Last modified: June 20, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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