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Protein

E3 ubiquitin-protein ligase KCMF1

Gene

KCMF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has intrinsic E3 ubiquitin ligase activity and promotes ubiquitination.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri3 – 50ZZ-typePROSITE-ProRule annotationAdd BLAST48
Zinc fingeri78 – 101C2H2-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase KCMF1 (EC:2.3.2.27)
Alternative name(s):
FGF-induced in gastric cancer
Potassium channel modulatory factor
Short name:
PCMF
RING-type E3 ubiquitin transferase KCMF1Curated
ZZ-type zinc finger-containing protein 1
Gene namesi
Name:KCMF1
Synonyms:FIGC, ZZZ1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000176407.17
HGNCiHGNC:20589 KCMF1
MIMi614719 gene
neXtProtiNX_Q9P0J7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi56888
OpenTargetsiENSG00000176407
PharmGKBiPA134967694

Polymorphism and mutation databases

BioMutaiKCMF1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00003492192 – 381E3 ubiquitin-protein ligase KCMF1Add BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2PhosphoserineCombined sources1
Modified residuei169PhosphoserineCombined sources1
Modified residuei189PhosphoserineCombined sources1
Modified residuei212PhosphoserineCombined sources1
Modified residuei335PhosphoserineCombined sources1
Modified residuei336PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9P0J7
MaxQBiQ9P0J7
PaxDbiQ9P0J7
PeptideAtlasiQ9P0J7
PRIDEiQ9P0J7
ProteomicsDBi83555

PTM databases

iPTMnetiQ9P0J7
PhosphoSitePlusiQ9P0J7

Expressioni

Tissue specificityi

Spleen, small intestine, ovary, peripheral blood, lung, kidney and pancreas. Expressed at low levels in the thymus, prostate, testis, colon, heart, brain, placenta and liver.1 Publication

Inductioni

Up-regulated by FGF2 in gastric cancer cells.1 Publication

Gene expression databases

BgeeiENSG00000176407 Expressed in 229 organ(s), highest expression level in sperm
CleanExiHS_KCMF1
ExpressionAtlasiQ9P0J7 baseline and differential
GenevisibleiQ9P0J7 HS

Organism-specific databases

HPAiHPA030383
HPA030384

Interactioni

Protein-protein interaction databases

BioGridi121218, 69 interactors
CORUMiQ9P0J7
DIPiDIP-47300N
IntActiQ9P0J7, 13 interactors
MINTiQ9P0J7
STRINGi9606.ENSP00000386738

Structurei

3D structure databases

ProteinModelPortaliQ9P0J7
SMRiQ9P0J7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili225 – 257Sequence analysisAdd BLAST33

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi333 – 336Poly-Ser4
Compositional biasi376 – 380Poly-Pro5

Sequence similaritiesi

Belongs to the KCMF1 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri3 – 50ZZ-typePROSITE-ProRule annotationAdd BLAST48
Zinc fingeri78 – 101C2H2-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG1280 Eukaryota
ENOG410XRX5 LUCA
GeneTreeiENSGT00510000047171
HOGENOMiHOG000007727
HOVERGENiHBG101229
InParanoidiQ9P0J7
KOiK22376
OMAiAVDGRNQ
OrthoDBiEOG091G09BU
PhylomeDBiQ9P0J7
TreeFamiTF318128

Family and domain databases

InterProiView protein in InterPro
IPR008598 Di19_Zn_binding_dom
IPR039858 KCMF1
IPR013087 Znf_C2H2_type
IPR000433 Znf_ZZ
PANTHERiPTHR12268 PTHR12268, 1 hit
PfamiView protein in Pfam
PF05605 zf-Di19, 1 hit
PF00569 ZZ, 1 hit
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 1 hit
SM00291 ZnF_ZZ, 1 hit
PROSITEiView protein in PROSITE
PS01357 ZF_ZZ_1, 1 hit
PS50135 ZF_ZZ_2, 1 hit
PS50157 ZINC_FINGER_C2H2_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9P0J7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSRHEGVSCD ACLKGNFRGR RYKCLICYDY DLCASCYESG ATTTRHTTDH
60 70 80 90 100
PMQCILTRVD FDLYYGGEAF SVEQPQSFTC PYCGKMGYTE TSLQEHVTSE
110 120 130 140 150
HAETSTEVIC PICAALPGGD PNHVTDDFAA HLTLEHRAPR DLDESSGVRH
160 170 180 190 200
VRRMFHPGRG LGGPRARRSN MHFTSSSTGG LSSSQSSYSP SNREAMDPIA
210 220 230 240 250
ELLSQLSGVR RSAGGQLNSS GPSASQLQQL QMQLQLERQH AQAARQQLET
260 270 280 290 300
ARNATRRTNT SSVTTTITQS TATTNIANTE SSQQTLQNSQ FLLTRLNDPK
310 320 330 340 350
MSETERQSME SERADRSLFV QELLLSTLVR EESSSSDEDD RGEMADFGAM
360 370 380
GCVDIMPLDV ALENLNLKES NKGNEPPPPP L
Length:381
Mass (Da):41,945
Last modified:September 2, 2008 - v2
Checksum:i23B2279C56CF199B
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9J3I2C9J3I2_HUMAN
E3 ubiquitin-protein ligase KCMF1
KCMF1
149Annotation score:
C9JSW5C9JSW5_HUMAN
E3 ubiquitin-protein ligase KCMF1
KCMF1
47Annotation score:
A0A1D5RMQ3A0A1D5RMQ3_HUMAN
E3 ubiquitin-protein ligase KCMF1
KCMF1
10Annotation score:

Sequence cautioni

The sequence AAX88897 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti95E → G in BAB14563 (PubMed:14702039).Curated1
Sequence conflicti242Q → R in BAC43745 (PubMed:15581609).Curated1
Sequence conflicti242Q → R in AAF67009 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083199 mRNA Translation: BAC43745.1
AF155652 mRNA Translation: AAF67009.1
AK023403 mRNA Translation: BAB14563.1
AK314761 mRNA Translation: BAG37299.1
AC022210 Genomic DNA Translation: AAY24192.1
AC078974 Genomic DNA Translation: AAX88897.1 Different initiation.
BC000178 mRNA Translation: AAH00178.1
AL122115 mRNA Translation: CAB59274.1
CCDSiCCDS46350.1
PIRiT34540
RefSeqiNP_064507.3, NM_020122.4
UniGeneiHs.654968

Genome annotation databases

EnsembliENST00000409785; ENSP00000386738; ENSG00000176407
GeneIDi56888
KEGGihsa:56888
UCSCiuc002sox.5 human

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083199 mRNA Translation: BAC43745.1
AF155652 mRNA Translation: AAF67009.1
AK023403 mRNA Translation: BAB14563.1
AK314761 mRNA Translation: BAG37299.1
AC022210 Genomic DNA Translation: AAY24192.1
AC078974 Genomic DNA Translation: AAX88897.1 Different initiation.
BC000178 mRNA Translation: AAH00178.1
AL122115 mRNA Translation: CAB59274.1
CCDSiCCDS46350.1
PIRiT34540
RefSeqiNP_064507.3, NM_020122.4
UniGeneiHs.654968

3D structure databases

ProteinModelPortaliQ9P0J7
SMRiQ9P0J7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121218, 69 interactors
CORUMiQ9P0J7
DIPiDIP-47300N
IntActiQ9P0J7, 13 interactors
MINTiQ9P0J7
STRINGi9606.ENSP00000386738

PTM databases

iPTMnetiQ9P0J7
PhosphoSitePlusiQ9P0J7

Polymorphism and mutation databases

BioMutaiKCMF1

Proteomic databases

EPDiQ9P0J7
MaxQBiQ9P0J7
PaxDbiQ9P0J7
PeptideAtlasiQ9P0J7
PRIDEiQ9P0J7
ProteomicsDBi83555

Protocols and materials databases

DNASUi56888
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000409785; ENSP00000386738; ENSG00000176407
GeneIDi56888
KEGGihsa:56888
UCSCiuc002sox.5 human

Organism-specific databases

CTDi56888
DisGeNETi56888
EuPathDBiHostDB:ENSG00000176407.17
GeneCardsiKCMF1
H-InvDBiHIX0020753
HGNCiHGNC:20589 KCMF1
HPAiHPA030383
HPA030384
MIMi614719 gene
neXtProtiNX_Q9P0J7
OpenTargetsiENSG00000176407
PharmGKBiPA134967694
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1280 Eukaryota
ENOG410XRX5 LUCA
GeneTreeiENSGT00510000047171
HOGENOMiHOG000007727
HOVERGENiHBG101229
InParanoidiQ9P0J7
KOiK22376
OMAiAVDGRNQ
OrthoDBiEOG091G09BU
PhylomeDBiQ9P0J7
TreeFamiTF318128

Enzyme and pathway databases

ReactomeiR-HSA-6798695 Neutrophil degranulation

Miscellaneous databases

ChiTaRSiKCMF1 human
GenomeRNAii56888
PROiPR:Q9P0J7
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000176407 Expressed in 229 organ(s), highest expression level in sperm
CleanExiHS_KCMF1
ExpressionAtlasiQ9P0J7 baseline and differential
GenevisibleiQ9P0J7 HS

Family and domain databases

InterProiView protein in InterPro
IPR008598 Di19_Zn_binding_dom
IPR039858 KCMF1
IPR013087 Znf_C2H2_type
IPR000433 Znf_ZZ
PANTHERiPTHR12268 PTHR12268, 1 hit
PfamiView protein in Pfam
PF05605 zf-Di19, 1 hit
PF00569 ZZ, 1 hit
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 1 hit
SM00291 ZnF_ZZ, 1 hit
PROSITEiView protein in PROSITE
PS01357 ZF_ZZ_1, 1 hit
PS50135 ZF_ZZ_2, 1 hit
PS50157 ZINC_FINGER_C2H2_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKCMF1_HUMAN
AccessioniPrimary (citable) accession number: Q9P0J7
Secondary accession number(s): Q4ZG04
, Q53SC7, Q9BWK2, Q9H8P5, Q9UFE8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 2, 2008
Last modified: November 7, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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