UniProtKB - Q9P035 (HACD3_HUMAN)
Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3
HACD3
Functioni
Caution
Catalytic activityi
- EC:4.2.1.1341 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Kineticsi
- KM=49.5 µM for 3-hydroxypalmitoyl-CoA (at 37 degrees Celsius)1 Publication
: fatty acid biosynthesis Pathwayi
This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 286 | By similarity | 1 | |
Active sitei | 293 | By similarity | 1 |
GO - Molecular functioni
- 3-hydroxyacyl-CoA dehydratase activity Source: UniProtKB
- 3-hydroxy-arachidoyl-CoA dehydratase activity Source: UniProtKB-EC
- 3-hydroxy-behenoyl-CoA dehydratase activity Source: UniProtKB-EC
- 3-hydroxy-lignoceroyl-CoA dehydratase activity Source: UniProtKB-EC
- enzyme binding Source: UniProtKB
- GTPase activator activity Source: ProtInc
- very-long-chain 3-hydroxyacyl-CoA dehydratase activity Source: UniProtKB-EC
GO - Biological processi
- activation of JUN kinase activity Source: ProtInc
- fatty acid elongation Source: UniProtKB
- I-kappaB kinase/NF-kappaB signaling Source: ProtInc
- positive regulation by virus of viral protein levels in host cell Source: AgBase
- positive regulation of viral genome replication Source: AgBase
- Rac protein signal transduction Source: UniProtKB
- small GTPase mediated signal transduction Source: ProtInc
- sphingolipid biosynthetic process Source: GO_Central
- very long-chain fatty acid biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Lyase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
PathwayCommonsi | Q9P035 |
Reactomei | R-HSA-75876, Synthesis of very long-chain fatty acyl-CoAs |
UniPathwayi | UPA00094 |
Chemistry databases
SwissLipidsi | SLP:000000439 |
Names & Taxonomyi
Protein namesi | Recommended name: Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3Curated (EC:4.2.1.1341 Publication)Alternative name(s): 3-hydroxyacyl-CoA dehydratase 31 Publication Short name: HACD31 Publication Butyrate-induced protein 11 Publication Short name: B-ind11 Publication Short name: hB-ind11 Publication Protein-tyrosine phosphatase-like A domain-containing protein 1Imported |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:24175, HACD3 |
MIMi | 615940, gene |
neXtProti | NX_Q9P035 |
VEuPathDBi | HostDB:ENSG00000074696.12 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- integral component of endoplasmic reticulum membrane Source: GO_Central
Nucleus
- nuclear membrane Source: HPA
Other locations
- focal adhesion Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 149 | CytoplasmicSequence analysisAdd BLAST | 149 | |
Transmembranei | 150 – 170 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 171 – 185 | LumenalSequence analysisAdd BLAST | 15 | |
Transmembranei | 186 – 207 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 208 – 217 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 218 – 235 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 236 – 241 | LumenalSequence analysis | 6 | |
Transmembranei | 242 – 256 | HelicalSequence analysisAdd BLAST | 15 | |
Topological domaini | 257 – 279 | CytoplasmicSequence analysisAdd BLAST | 23 | |
Transmembranei | 280 – 298 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 299 – 322 | LumenalSequence analysisAdd BLAST | 24 | |
Transmembranei | 323 – 343 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 344 – 362 | CytoplasmicSequence analysisAdd BLAST | 19 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 51495 |
OpenTargetsi | ENSG00000074696 |
PharmGKBi | PA142671113 |
Miscellaneous databases
Pharosi | Q9P035, Tbio |
Genetic variation databases
BioMutai | HACD3 |
DMDMi | 166199462 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000313724 | 1 – 362 | Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3Add BLAST | 362 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 7 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 114 | PhosphoserineCombined sources | 1 | |
Modified residuei | 135 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9P035 |
jPOSTi | Q9P035 |
MassIVEi | Q9P035 |
MaxQBi | Q9P035 |
PaxDbi | Q9P035 |
PeptideAtlasi | Q9P035 |
PRIDEi | Q9P035 |
ProteomicsDBi | 4949 83540 [Q9P035-1] |
TopDownProteomicsi | Q9P035-1 [Q9P035-1] |
PTM databases
iPTMneti | Q9P035 |
PhosphoSitePlusi | Q9P035 |
SwissPalmi | Q9P035 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000074696, Expressed in pituitary gland and 232 other tissues |
ExpressionAtlasi | Q9P035, baseline and differential |
Genevisiblei | Q9P035, HS |
Organism-specific databases
HPAi | ENSG00000074696, Low tissue specificity |
Interactioni
Subunit structurei
May interact with enzymes of the ELO family (including ELOVL1); with those enzymes that mediate condensation, the first of the four steps of the reaction cycle responsible for fatty acids elongation, may be part of a larger fatty acids elongase complex (PubMed:18554506).
Interacts with RAC1 (PubMed:10747961). Associates with internalized insulin receptor/INSR complexes on Golgi/endosomal membranes; HACD3/PTPLAD1 together with ATIC and PRKAA2/AMPK2 is proposed to be part of a signaling network regulating INSR autophosphorylation and endocytosis (PubMed:25687571).
3 PublicationsBinary interactionsi
Hide detailsQ9P035
GO - Molecular functioni
- enzyme binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 119570, 122 interactors |
IntActi | Q9P035, 71 interactors |
MINTi | Q9P035 |
STRINGi | 9606.ENSP00000261875 |
Miscellaneous databases
RNActi | Q9P035, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 94 | CSPROSITE-ProRule annotationAdd BLAST | 90 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 111 – 136 | Sequence analysisAdd BLAST | 26 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 350 – 353 | Poly-Arg | 4 | |
Compositional biasi | 357 – 360 | Poly-Lys | 4 |
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3187, Eukaryota |
GeneTreei | ENSGT00530000062962 |
HOGENOMi | CLU_046712_0_0_1 |
InParanoidi | Q9P035 |
OrthoDBi | 1458293at2759 |
PhylomeDBi | Q9P035 |
TreeFami | TF313326 |
Family and domain databases
Gene3Di | 2.60.40.790, 1 hit |
InterProi | View protein in InterPro IPR007052, CS_dom IPR008978, HSP20-like_chaperone IPR007482, Tyr_Pase-like_PTPLA |
PANTHERi | PTHR11035, PTHR11035, 1 hit |
Pfami | View protein in Pfam PF04387, PTPLA, 1 hit |
SUPFAMi | SSF49764, SSF49764, 1 hit |
PROSITEi | View protein in PROSITE PS51203, CS, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MENQVLTPHV YWAQRHRELY LRVELSDVQN PAISITENVL HFKAQGHGAK
60 70 80 90 100
GDNVYEFHLE FLDLVKPEPV YKLTQRQVNI TVQKKVSQWW ERLTKQEKRP
110 120 130 140 150
LFLAPDFDRW LDESDAEMEL RAKEEERLNK LRLESEGSPE TLTNLRKGYL
160 170 180 190 200
FMYNLVQFLG FSWIFVNLTV RFCILGKESF YDTFHTVADM MYFCQMLAVV
210 220 230 240 250
ETINAAIGVT TSPVLPSLIQ LLGRNFILFI IFGTMEEMQN KAVVFFVFYL
260 270 280 290 300
WSAIEIFRYS FYMLTCIDMD WKVLTWLRYT LWIPLYPLGC LAEAVSVIQS
310 320 330 340 350
IPIFNETGRF SFTLPYPVKI KVRFSFFLQI YLIMIFLGLY INFRHLYKQR
360
RRRYGQKKKK IH
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BS72 | H3BS72_HUMAN | Very-long-chain (3R)-3-hydroxyacyl-... | HACD3 | 400 | Annotation score: | ||
H3BPZ1 | H3BPZ1_HUMAN | Very-long-chain (3R)-3-hydroxyacyl-... | HACD3 | 337 | Annotation score: | ||
H3BRL8 | H3BRL8_HUMAN | Very-long-chain (3R)-3-hydroxyacyl-... | HACD3 | 245 | Annotation score: | ||
H3BMZ1 | H3BMZ1_HUMAN | Very-long-chain (3R)-3-hydroxyacyl-... | HACD3 | 139 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 60 | E → K in BAB55101 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 245 | F → I in BAC11277 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 282 | W → G in AAH35508 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 292 | A → V in AAF29085 (PubMed:11042152).Curated | 1 | |
Sequence conflicti | 352 – 362 | RRYGQKKKKIH → LKMRAGAVAHACDPSALGG in CAB69070 (PubMed:10747961).CuratedAdd BLAST | 11 | |
Sequence conflicti | 361 | I → K in AAH35508 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_037712 | 56 | E → K. Corresponds to variant dbSNP:rs11632737Ensembl. | 1 | |
Natural variantiVAR_037713 | 269 | M → L. Corresponds to variant dbSNP:rs2279854Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056070 | 69 – 123 | Missing in isoform 2. 1 PublicationAdd BLAST | 55 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ271091 mRNA Translation: CAB69070.1 AF161470 mRNA Translation: AAF29085.1 Frameshift. AK027421 mRNA Translation: BAB55101.1 AK074857 mRNA Translation: BAC11249.1 Frameshift. AK074898 mRNA Translation: BAC11277.1 AK299234 mRNA Translation: BAG61266.1 AC011846 Genomic DNA No translation available. AC027220 Genomic DNA No translation available. BC019873 mRNA Translation: AAH19873.1 BC035508 mRNA Translation: AAH35508.1 BC047685 mRNA Translation: AAH47685.1 BC058912 mRNA Translation: AAH58912.1 DQ251107 Genomic DNA Translation: ABB83547.1 |
CCDSi | CCDS45282.1 [Q9P035-1] |
RefSeqi | NP_057479.2, NM_016395.2 [Q9P035-1] |
Genome annotation databases
Ensembli | ENST00000261875; ENSP00000261875; ENSG00000074696 [Q9P035-1] ENST00000442729; ENSP00000392491; ENSG00000074696 [Q9P035-2] |
GeneIDi | 51495 |
KEGGi | hsa:51495 |
UCSCi | uc002apc.4, human [Q9P035-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ271091 mRNA Translation: CAB69070.1 AF161470 mRNA Translation: AAF29085.1 Frameshift. AK027421 mRNA Translation: BAB55101.1 AK074857 mRNA Translation: BAC11249.1 Frameshift. AK074898 mRNA Translation: BAC11277.1 AK299234 mRNA Translation: BAG61266.1 AC011846 Genomic DNA No translation available. AC027220 Genomic DNA No translation available. BC019873 mRNA Translation: AAH19873.1 BC035508 mRNA Translation: AAH35508.1 BC047685 mRNA Translation: AAH47685.1 BC058912 mRNA Translation: AAH58912.1 DQ251107 Genomic DNA Translation: ABB83547.1 |
CCDSi | CCDS45282.1 [Q9P035-1] |
RefSeqi | NP_057479.2, NM_016395.2 [Q9P035-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 119570, 122 interactors |
IntActi | Q9P035, 71 interactors |
MINTi | Q9P035 |
STRINGi | 9606.ENSP00000261875 |
Chemistry databases
SwissLipidsi | SLP:000000439 |
PTM databases
iPTMneti | Q9P035 |
PhosphoSitePlusi | Q9P035 |
SwissPalmi | Q9P035 |
Genetic variation databases
BioMutai | HACD3 |
DMDMi | 166199462 |
Proteomic databases
EPDi | Q9P035 |
jPOSTi | Q9P035 |
MassIVEi | Q9P035 |
MaxQBi | Q9P035 |
PaxDbi | Q9P035 |
PeptideAtlasi | Q9P035 |
PRIDEi | Q9P035 |
ProteomicsDBi | 4949 83540 [Q9P035-1] |
TopDownProteomicsi | Q9P035-1 [Q9P035-1] |
Protocols and materials databases
Antibodypediai | 7067, 111 antibodies |
Genome annotation databases
Ensembli | ENST00000261875; ENSP00000261875; ENSG00000074696 [Q9P035-1] ENST00000442729; ENSP00000392491; ENSG00000074696 [Q9P035-2] |
GeneIDi | 51495 |
KEGGi | hsa:51495 |
UCSCi | uc002apc.4, human [Q9P035-1] |
Organism-specific databases
CTDi | 51495 |
DisGeNETi | 51495 |
GeneCardsi | HACD3 |
HGNCi | HGNC:24175, HACD3 |
HPAi | ENSG00000074696, Low tissue specificity |
MIMi | 615940, gene |
neXtProti | NX_Q9P035 |
OpenTargetsi | ENSG00000074696 |
PharmGKBi | PA142671113 |
VEuPathDBi | HostDB:ENSG00000074696.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3187, Eukaryota |
GeneTreei | ENSGT00530000062962 |
HOGENOMi | CLU_046712_0_0_1 |
InParanoidi | Q9P035 |
OrthoDBi | 1458293at2759 |
PhylomeDBi | Q9P035 |
TreeFami | TF313326 |
Enzyme and pathway databases
UniPathwayi | UPA00094 |
PathwayCommonsi | Q9P035 |
Reactomei | R-HSA-75876, Synthesis of very long-chain fatty acyl-CoAs |
Miscellaneous databases
BioGRID-ORCSi | 51495, 4 hits in 855 CRISPR screens |
ChiTaRSi | HACD3, human |
GeneWikii | PTPLAD1 |
GenomeRNAii | 51495 |
Pharosi | Q9P035, Tbio |
PROi | PR:Q9P035 |
RNActi | Q9P035, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000074696, Expressed in pituitary gland and 232 other tissues |
ExpressionAtlasi | Q9P035, baseline and differential |
Genevisiblei | Q9P035, HS |
Family and domain databases
Gene3Di | 2.60.40.790, 1 hit |
InterProi | View protein in InterPro IPR007052, CS_dom IPR008978, HSP20-like_chaperone IPR007482, Tyr_Pase-like_PTPLA |
PANTHERi | PTHR11035, PTHR11035, 1 hit |
Pfami | View protein in Pfam PF04387, PTPLA, 1 hit |
SUPFAMi | SSF49764, SSF49764, 1 hit |
PROSITEi | View protein in PROSITE PS51203, CS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HACD3_HUMAN | |
Accessioni | Q9P035Primary (citable) accession number: Q9P035 Secondary accession number(s): A0PJA1 Q9NQA7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | January 15, 2008 | |
Last modified: | February 10, 2021 | |
This is version 155 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 15
Human chromosome 15: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families