UniProtKB - Q9NZZ3 (CHMP5_HUMAN)
Charged multivesicular body protein 5
CHMP5
Functioni
Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses) (PubMed:14519844).
ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release (PubMed:14519844).
1 PublicationGO - Molecular functioni
- cadherin binding Source: BHF-UCL
GO - Biological processi
- autophagosome maturation Source: ComplexPortal
- autophagy Source: ComplexPortal
- cellular response to lipopolysaccharide Source: UniProtKB
- cellular response to muramyl dipeptide Source: UniProtKB
- ESCRT III complex disassembly Source: ParkinsonsUK-UCL
- late endosome to lysosome transport Source: ComplexPortal
- late endosome to vacuole transport via multivesicular body sorting pathway Source: GO_Central
- membrane fission Source: ComplexPortal
- midbody abscission Source: UniProtKB
- mitotic metaphase plate congression Source: UniProtKB
- multivesicular body assembly Source: ParkinsonsUK-UCL
- multivesicular body-lysosome fusion Source: ComplexPortal
- multivesicular body sorting pathway Source: ComplexPortal
- negative regulation of cell death Source: ComplexPortal
- nuclear membrane reassembly Source: ComplexPortal
- nucleus organization Source: UniProtKB
- plasma membrane repair Source: ComplexPortal
- protein transport Source: UniProtKB-KW
- regulation of centrosome duplication Source: UniProtKB
- regulation of mitotic spindle assembly Source: UniProtKB
- regulation of receptor recycling Source: Ensembl
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: ComplexPortal
- vesicle budding from membrane Source: GO_Central
- vesicle fusion with vacuole Source: ComplexPortal
- viral budding Source: UniProtKB
- viral budding from plasma membrane Source: ComplexPortal
- viral budding via host ESCRT complex Source: ComplexPortal
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q9NZZ3 |
Reactomei | R-HSA-162588, Budding and maturation of HIV virion R-HSA-917729, Endosomal Sorting Complex Required For Transport (ESCRT) |
SignaLinki | Q9NZZ3 |
SIGNORi | Q9NZZ3 |
Names & Taxonomyi
Protein namesi | Recommended name: Charged multivesicular body protein 5Alternative name(s): Chromatin-modifying protein 5 SNF7 domain-containing protein 2 Vacuolar protein sorting-associated protein 60 Short name: Vps60 Short name: hVps60 |
Gene namesi | Name:CHMP5 Synonyms:C9orf83, SNF7DC2 ORF Names:CGI-341 Publication, HSPC177, PNAS-114, PNAS-2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:26942, CHMP5 |
MIMi | 610900, gene |
neXtProti | NX_Q9NZZ3 |
VEuPathDBi | HostDB:ENSG00000086065 |
Subcellular locationi
Endosome
- Endosome membrane Curated; Peripheral membrane protein Curated
Cytoplasm and Cytosol
- cytosol 1 Publication
Other locations
- Midbody 1 Publication
Note: Localizes to the midbody of dividing cells (PubMed:17853893). Localized in two distinct rings on either side of the Flemming body (PubMed:17853893).1 Publication
Cytoskeleton
- kinetochore microtubule Source: ComplexPortal
Cytosol
- cytosol Source: UniProtKB
Endosome
- multivesicular body Source: GO_Central
- multivesicular body membrane Source: ComplexPortal
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Lysosome
- lysosomal membrane Source: ComplexPortal
Nucleus
- nuclear pore Source: ComplexPortal
Plasma Membrane
- plasma membrane Source: ComplexPortal
Other locations
- amphisome membrane Source: ComplexPortal
- autophagosome membrane Source: ComplexPortal
- kinetochore Source: ComplexPortal
- midbody Source: ComplexPortal
Keywords - Cellular componenti
Cytoplasm, Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 7 – 11 | KAKPK → RARPR: Decreased stearoylation in response to S.flexneri infection. 1 Publication | 5 | |
Mutagenesisi | 7 | K → R: Decreased stearoylation in response to S.flexneri infection. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 51510 |
OpenTargetsi | ENSG00000086065 |
PharmGKBi | PA134903143 |
Miscellaneous databases
Pharosi | Q9NZZ3, Tbio |
Genetic variation databases
BioMutai | CHMP5 |
DMDMi | 51702157 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000211500 | 1 – 219 | Charged multivesicular body protein 5Add BLAST | 219 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 7 | (Microbial infection) N6-stearoyl lysine1 Publication | 1 | |
Modified residuei | 86 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9NZZ3 |
jPOSTi | Q9NZZ3 |
MassIVEi | Q9NZZ3 |
MaxQBi | Q9NZZ3 |
PaxDbi | Q9NZZ3 |
PeptideAtlasi | Q9NZZ3 |
PRIDEi | Q9NZZ3 |
ProteomicsDBi | 83526 [Q9NZZ3-1] 83527 [Q9NZZ3-2] |
TopDownProteomicsi | Q9NZZ3-1 [Q9NZZ3-1] |
PTM databases
GlyGeni | Q9NZZ3, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9NZZ3 |
MetOSitei | Q9NZZ3 |
PhosphoSitePlusi | Q9NZZ3 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSG00000086065, Expressed in caudate nucleus and 245 other tissues |
Genevisiblei | Q9NZZ3, HS |
Organism-specific databases
HPAi | ENSG00000086065, Low tissue specificity |
Interactioni
Subunit structurei
Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentially.
Interacts with VTA1; the interaction involves soluble CHMP5 (PubMed:15644320, PubMed:17261583, PubMed:21543490).
Interacts with CHMP2A (PubMed:14519844).
Interacts with NOD2 (PubMed:27812135).
5 PublicationsBinary interactionsi
Q9NZZ3
With | #Exp. | IntAct |
---|---|---|
CHMP4B [Q9H444] | 4 | EBI-751303,EBI-749627 |
MITD1 [Q8WV92] | 3 | EBI-751303,EBI-2691489 |
STAMBP [O95630] | 2 | EBI-751303,EBI-396676 |
VPS4B [O75351] | 6 | EBI-751303,EBI-2514459 |
VTA1 [Q9NP79] | 3 | EBI-751303,EBI-740160 |
Isoform 1 [Q9NZZ3-1]
With | #Exp. | IntAct |
---|---|---|
BROX [Q5VW32] | 6 | EBI-15979532,EBI-6286053 |
GO - Molecular functioni
- cadherin binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 119579, 63 interactors |
ComplexPortali | CPX-329, ESCRT-III complex |
CORUMi | Q9NZZ3 |
DIPi | DIP-50420N |
IntActi | Q9NZZ3, 50 interactors |
MINTi | Q9NZZ3 |
STRINGi | 9606.ENSP00000223500 |
Miscellaneous databases
RNActi | Q9NZZ3, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9NZZ3 |
BMRBi | Q9NZZ3 |
SMRi | Q9NZZ3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 21 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 121 – 158 | Interaction with VTA1Add BLAST | 38 | |
Regioni | 188 – 219 | DisorderedSequence analysisAdd BLAST | 32 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 26 – 179 | Sequence analysisAdd BLAST | 154 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG1655, Eukaryota |
GeneTreei | ENSGT00550000074817 |
HOGENOMi | CLU_079409_0_0_1 |
InParanoidi | Q9NZZ3 |
OMAi | MEQAQTM |
OrthoDBi | 1301560at2759 |
PhylomeDBi | Q9NZZ3 |
TreeFami | TF300122 |
Family and domain databases
InterProi | View protein in InterPro IPR005024, Snf7_fam |
PANTHERi | PTHR22761, PTHR22761, 1 hit |
Pfami | View protein in Pfam PF03357, Snf7, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNRLFGKAKP KAPPPSLTDC IGTVDSRAES IDKKISRLDA ELVKYKDQIK
60 70 80 90 100
KMREGPAKNM VKQKALRVLK QKRMYEQQRD NLAQQSFNME QANYTIQSLK
110 120 130 140 150
DTKTTVDAMK LGVKEMKKAY KQVKIDQIED LQDQLEDMME DANEIQEALS
160 170 180 190 200
RSYGTPELDE DDLEAELDAL GDELLADEDS SYLDEAASAP AIPEGVPTDT
210
KNKDGVLVDE FGLPQIPAS
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 14 | P → R in AAD27743 (PubMed:10810093).Curated | 1 | |
Sequence conflicti | 19 | D → G in AAH16698 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 84 – 93 | QQSFNMEQAN → NSHSTWTGH in AAD27743 (PubMed:10810093).Curated | 10 | |
Sequence conflicti | 122 | Q → P in AAD27743 (PubMed:10810093).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042556 | 166 – 219 | ELDAL…QIPAS → GWSSGG in isoform 2. 1 PublicationAdd BLAST | 54 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF132968 mRNA Translation: AAD27743.1 AF161525 mRNA Translation: AAF29140.1 AK295744 mRNA Translation: BAG58578.1 AK315455 mRNA Translation: BAG37842.1 AL356472 Genomic DNA No translation available. CH471071 Genomic DNA Translation: EAW58512.1 BC006974 mRNA Translation: AAH06974.1 BC007457 mRNA Translation: AAH07457.1 BC016698 mRNA Translation: AAH16698.1 BC020796 mRNA Translation: AAH20796.1 BC021168 mRNA Translation: AAH21168.1 AF275810 mRNA Translation: AAG23821.1 Different initiation. AF229832 mRNA Translation: AAF42917.1 |
CCDSi | CCDS56569.1 [Q9NZZ3-2] CCDS6537.1 [Q9NZZ3-1] |
RefSeqi | NP_001182465.1, NM_001195536.1 [Q9NZZ3-2] NP_057494.3, NM_016410.5 [Q9NZZ3-1] |
Genome annotation databases
Ensembli | ENST00000223500.9; ENSP00000223500.7; ENSG00000086065.14 ENST00000419016.6; ENSP00000442725.1; ENSG00000086065.14 [Q9NZZ3-2] |
GeneIDi | 51510 |
KEGGi | hsa:51510 |
MANE-Selecti | ENST00000223500.9; ENSP00000223500.7; NM_016410.6; NP_057494.3 |
UCSCi | uc003zsm.5, human [Q9NZZ3-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF132968 mRNA Translation: AAD27743.1 AF161525 mRNA Translation: AAF29140.1 AK295744 mRNA Translation: BAG58578.1 AK315455 mRNA Translation: BAG37842.1 AL356472 Genomic DNA No translation available. CH471071 Genomic DNA Translation: EAW58512.1 BC006974 mRNA Translation: AAH06974.1 BC007457 mRNA Translation: AAH07457.1 BC016698 mRNA Translation: AAH16698.1 BC020796 mRNA Translation: AAH20796.1 BC021168 mRNA Translation: AAH21168.1 AF275810 mRNA Translation: AAG23821.1 Different initiation. AF229832 mRNA Translation: AAF42917.1 |
CCDSi | CCDS56569.1 [Q9NZZ3-2] CCDS6537.1 [Q9NZZ3-1] |
RefSeqi | NP_001182465.1, NM_001195536.1 [Q9NZZ3-2] NP_057494.3, NM_016410.5 [Q9NZZ3-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2LXM | NMR | - | B | 139-195 | [»] | |
3ULY | X-ray | 2.60 | B | 151-219 | [»] | |
3UM0 | X-ray | 3.10 | B | 200-219 | [»] | |
3UM1 | X-ray | 2.71 | B/E | 151-219 | [»] | |
3UM2 | X-ray | 2.59 | B/E | 200-219 | [»] | |
4TXR | X-ray | 1.00 | C | 139-195 | [»] | |
AlphaFoldDBi | Q9NZZ3 | |||||
BMRBi | Q9NZZ3 | |||||
SMRi | Q9NZZ3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 119579, 63 interactors |
ComplexPortali | CPX-329, ESCRT-III complex |
CORUMi | Q9NZZ3 |
DIPi | DIP-50420N |
IntActi | Q9NZZ3, 50 interactors |
MINTi | Q9NZZ3 |
STRINGi | 9606.ENSP00000223500 |
PTM databases
GlyGeni | Q9NZZ3, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9NZZ3 |
MetOSitei | Q9NZZ3 |
PhosphoSitePlusi | Q9NZZ3 |
Genetic variation databases
BioMutai | CHMP5 |
DMDMi | 51702157 |
Proteomic databases
EPDi | Q9NZZ3 |
jPOSTi | Q9NZZ3 |
MassIVEi | Q9NZZ3 |
MaxQBi | Q9NZZ3 |
PaxDbi | Q9NZZ3 |
PeptideAtlasi | Q9NZZ3 |
PRIDEi | Q9NZZ3 |
ProteomicsDBi | 83526 [Q9NZZ3-1] 83527 [Q9NZZ3-2] |
TopDownProteomicsi | Q9NZZ3-1 [Q9NZZ3-1] |
Protocols and materials databases
Antibodypediai | 25186, 234 antibodies from 28 providers |
DNASUi | 51510 |
Genome annotation databases
Ensembli | ENST00000223500.9; ENSP00000223500.7; ENSG00000086065.14 ENST00000419016.6; ENSP00000442725.1; ENSG00000086065.14 [Q9NZZ3-2] |
GeneIDi | 51510 |
KEGGi | hsa:51510 |
MANE-Selecti | ENST00000223500.9; ENSP00000223500.7; NM_016410.6; NP_057494.3 |
UCSCi | uc003zsm.5, human [Q9NZZ3-1] |
Organism-specific databases
CTDi | 51510 |
DisGeNETi | 51510 |
GeneCardsi | CHMP5 |
HGNCi | HGNC:26942, CHMP5 |
HPAi | ENSG00000086065, Low tissue specificity |
MIMi | 610900, gene |
neXtProti | NX_Q9NZZ3 |
OpenTargetsi | ENSG00000086065 |
PharmGKBi | PA134903143 |
VEuPathDBi | HostDB:ENSG00000086065 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1655, Eukaryota |
GeneTreei | ENSGT00550000074817 |
HOGENOMi | CLU_079409_0_0_1 |
InParanoidi | Q9NZZ3 |
OMAi | MEQAQTM |
OrthoDBi | 1301560at2759 |
PhylomeDBi | Q9NZZ3 |
TreeFami | TF300122 |
Enzyme and pathway databases
PathwayCommonsi | Q9NZZ3 |
Reactomei | R-HSA-162588, Budding and maturation of HIV virion R-HSA-917729, Endosomal Sorting Complex Required For Transport (ESCRT) |
SignaLinki | Q9NZZ3 |
SIGNORi | Q9NZZ3 |
Miscellaneous databases
BioGRID-ORCSi | 51510, 456 hits in 1050 CRISPR screens |
ChiTaRSi | CHMP5, human |
GeneWikii | CHMP5 |
GenomeRNAii | 51510 |
Pharosi | Q9NZZ3, Tbio |
PROi | PR:Q9NZZ3 |
RNActi | Q9NZZ3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000086065, Expressed in caudate nucleus and 245 other tissues |
Genevisiblei | Q9NZZ3, HS |
Family and domain databases
InterProi | View protein in InterPro IPR005024, Snf7_fam |
PANTHERi | PTHR22761, PTHR22761, 1 hit |
Pfami | View protein in Pfam PF03357, Snf7, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CHMP5_HUMAN | |
Accessioni | Q9NZZ3Primary (citable) accession number: Q9NZZ3 Secondary accession number(s): B2RD95 Q9Y323 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 31, 2004 |
Last sequence update: | October 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 174 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families