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Protein

Programmed cell death 1 ligand 1

Gene

CD274

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).4 Publications

GO - Biological processi

  • cell surface receptor signaling pathway Source: UniProtKB
  • immune response Source: UniProtKB
  • negative regulation of activated T cell proliferation Source: UniProtKB
  • negative regulation of CD8-positive, alpha-beta T cell activation Source: UniProtKB
  • negative regulation of interferon-gamma production Source: UniProtKB
  • negative regulation of interleukin-10 production Source: UniProtKB
  • negative regulation of tumor necrosis factor superfamily cytokine production Source: UniProtKB
  • positive regulation of activated CD8-positive, alpha-beta T cell apoptotic process Source: UniProtKB
  • positive regulation of cell migration Source: Ensembl
  • positive regulation of interleukin-10 secretion Source: UniProtKB
  • positive regulation of T cell proliferation Source: UniProtKB
  • positive regulation of tolerance induction to tumor cell Source: UniProtKB
  • response to cytokine Source: AgBase
  • signal transduction Source: UniProtKB
  • T cell costimulation Source: UniProtKB
  • toxin transport Source: Ensembl

Keywordsi

Molecular functionReceptor
Biological processImmunity

Enzyme and pathway databases

ReactomeiR-HSA-389948 PD-1 signaling
SIGNORiQ9NZQ7

Names & Taxonomyi

Protein namesi
Recommended name:
Programmed cell death 1 ligand 11 Publication
Short name:
PD-L12 Publications
Short name:
PDCD1 ligand 1
Short name:
Programmed death ligand 1
Alternative name(s):
B7 homolog 11 Publication
Short name:
B7-H11 Publication
CD_antigen: CD274
Gene namesi
Name:CD274Imported
Synonyms:B7H1, PDCD1L1, PDCD1LG1, PDL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000120217.13
HGNCiHGNC:17635 CD274
MIMi605402 gene
neXtProtiNX_Q9NZQ7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 238ExtracellularSequence analysisAdd BLAST220
Transmembranei239 – 259HelicalSequence analysisAdd BLAST21
Topological domaini260 – 290CytoplasmicSequence analysisAdd BLAST31

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi29126
OpenTargetsiENSG00000120217
PharmGKBiPA134915280

Chemistry databases

ChEMBLiCHEMBL3580522
DrugBankiDB11595 Atezolizumab

Polymorphism and mutation databases

BioMutaiCD274
DMDMi83287884

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000001455319 – 290Programmed cell death 1 ligand 1Add BLAST272

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi35N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi40 ↔ 114PROSITE-ProRule annotation1 Publication
Disulfide bondi155 ↔ 209PROSITE-ProRule annotation1 Publication
Glycosylationi192N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi200N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi219N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Ubiquitinated; STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ9NZQ7
PaxDbiQ9NZQ7
PeptideAtlasiQ9NZQ7
PRIDEiQ9NZQ7
ProteomicsDBi83483
83484 [Q9NZQ7-2]
83485 [Q9NZQ7-3]

PTM databases

iPTMnetiQ9NZQ7
PhosphoSitePlusiQ9NZQ7

Expressioni

Tissue specificityi

Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.2 Publications

Inductioni

Up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation. Up-regulated in B-cells activated by surface Ig cross-linking.3 Publications

Gene expression databases

BgeeiENSG00000120217
CleanExiHS_CD274
GenevisibleiQ9NZQ7 HS

Organism-specific databases

HPAiCAB030018
CAB076385

Interactioni

Subunit structurei

Interacts with PDCD1 (PubMed:11015443, PubMed:18287011). Interacts (via transmembrane domain) with CMTM4 and CMTM6 (PubMed:28813417, PubMed:28813410).4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi118891, 48 interactors
DIPiDIP-29731N
IntActiQ9NZQ7, 6 interactors
STRINGi9606.ENSP00000370989

Chemistry databases

BindingDBiQ9NZQ7

Structurei

Secondary structure

1290
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi26 – 31Combined sources6
Beta strandi36 – 41Combined sources6
Beta strandi45 – 47Combined sources3
Helixi50 – 52Combined sources3
Beta strandi54 – 59Combined sources6
Beta strandi62 – 68Combined sources7
Helixi74 – 76Combined sources3
Helixi79 – 81Combined sources3
Turni82 – 84Combined sources3
Beta strandi85 – 87Combined sources3
Helixi89 – 94Combined sources6
Beta strandi96 – 103Combined sources8
Helixi106 – 108Combined sources3
Beta strandi110 – 131Combined sources22
Beta strandi138 – 145Combined sources8
Turni146 – 149Combined sources4
Beta strandi150 – 161Combined sources12
Beta strandi164 – 169Combined sources6
Beta strandi177 – 183Combined sources7
Beta strandi185 – 187Combined sources3
Beta strandi191 – 200Combined sources10
Beta strandi206 – 213Combined sources8
Turni214 – 217Combined sources4
Beta strandi218 – 225Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BIKX-ray2.65A18-239[»]
3BISX-ray2.64A/B18-239[»]
3FN3X-ray2.70A/B19-238[»]
3SBWX-ray2.28C19-239[»]
4Z18X-ray1.95A/B19-239[»]
4ZQKX-ray2.45A18-132[»]
5C3TX-ray1.80A18-134[»]
5GGTX-ray2.80A18-134[»]
5GRJX-ray3.21A18-238[»]
5IUSX-ray2.89C/D18-239[»]
5J89X-ray2.20A/B/C/D2-134[»]
5J8OX-ray2.30A/B18-134[»]
5JDRX-ray2.70A/B18-239[»]
5JDSX-ray1.70A18-132[»]
5N2DX-ray2.35A/B/C/D2-134[»]
5N2FX-ray1.70A/B18-134[»]
5NIUX-ray2.01A/B/C/D18-134[»]
5NIXX-ray2.20A/B/C/D18-134[»]
5O45X-ray0.99A17-134[»]
5O4YX-ray2.30B/C/E18-132[»]
5X8LX-ray3.10A/B/C/D/E18-134[»]
5X8MX-ray2.66A18-134[»]
5XJ4X-ray2.30A19-238[»]
5XXYX-ray2.90A18-133[»]
ProteinModelPortaliQ9NZQ7
SMRiQ9NZQ7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NZQ7

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 127Ig-like V-typeAdd BLAST109
Domaini133 – 225Ig-like C2-typeAdd BLAST93

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIBU Eukaryota
ENOG4111V14 LUCA
GeneTreeiENSGT00650000093373
HOGENOMiHOG000130048
HOVERGENiHBG082112
InParanoidiQ9NZQ7
KOiK06745
OMAiIWTSSDH
OrthoDBiEOG091G0F5W
PhylomeDBiQ9NZQ7
TreeFamiTF331083

Family and domain databases

Gene3Di2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR013162 CD80_C2-set
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR013106 Ig_V-set
PfamiView protein in Pfam
PF08205 C2-set_2, 1 hit
PF07686 V-set, 1 hit
SMARTiView protein in SMART
SM00409 IG, 2 hits
SUPFAMiSSF48726 SSF48726, 2 hits
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 2 hits

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NZQ7-1) [UniParc]FASTAAdd to basket
Also known as: PD-L1I

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRIFAVFIFM TYWHLLNAFT VTVPKDLYVV EYGSNMTIEC KFPVEKQLDL
60 70 80 90 100
AALIVYWEME DKNIIQFVHG EEDLKVQHSS YRQRARLLKD QLSLGNAALQ
110 120 130 140 150
ITDVKLQDAG VYRCMISYGG ADYKRITVKV NAPYNKINQR ILVVDPVTSE
160 170 180 190 200
HELTCQAEGY PKAEVIWTSS DHQVLSGKTT TTNSKREEKL FNVTSTLRIN
210 220 230 240 250
TTTNEIFYCT FRRLDPEENH TAELVIPELP LAHPPNERTH LVILGAILLC
260 270 280 290
LGVALTFIFR LRKGRMMDVK KCGIQDTNSK KQSDTHLEET
Length:290
Mass (Da):33,275
Last modified:October 1, 2000 - v1
Checksum:iFE957086E62A31A8
GO
Isoform 2 (identifier: Q9NZQ7-2) [UniParc]FASTAAdd to basket
Also known as: PD-L1II

The sequence of this isoform differs from the canonical sequence as follows:
     19-132: Missing.

Show »
Length:176
Mass (Da):20,246
Checksum:iB8BD0CFF9DEA722F
GO
Isoform 3 (identifier: Q9NZQ7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     178-178: K → D
     179-290: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:178
Mass (Da):20,454
Checksum:iA306BF2018885F0B
GO

Sequence cautioni

The sequence BAA91966 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01373519 – 132Missing in isoform 2. 2 PublicationsAdd BLAST114
Alternative sequenceiVSP_013736178K → D in isoform 3. 2 Publications1
Alternative sequenceiVSP_013737179 – 290Missing in isoform 3. 2 PublicationsAdd BLAST112

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177937 mRNA Translation: AAF25807.1
AF233516 mRNA Translation: AAG18508.1
AY254342 mRNA Translation: AAP13470.1
AY291313 mRNA Translation: AAP42144.1
AY714881 mRNA Translation: AAU09634.1
DQ286582 mRNA Translation: ABB90152.1
AK001894 mRNA Translation: BAA91966.1 Different initiation.
AK300470 mRNA Translation: BAG62189.1
AK314567 mRNA Translation: BAG37149.1
AL162253 Genomic DNA No translation available.
CH471071 Genomic DNA Translation: EAW58763.1
BC069381 mRNA Translation: AAH69381.1
BC074984 mRNA Translation: AAH74984.1
BC113734 mRNA Translation: AAI13735.1
BC113736 mRNA Translation: AAI13737.1
CCDSiCCDS59118.1 [Q9NZQ7-2]
CCDS6464.1 [Q9NZQ7-1]
RefSeqiNP_001254635.1, NM_001267706.1 [Q9NZQ7-2]
NP_054862.1, NM_014143.3 [Q9NZQ7-1]
UniGeneiHs.521989

Genome annotation databases

EnsembliENST00000381573; ENSP00000370985; ENSG00000120217 [Q9NZQ7-2]
ENST00000381577; ENSP00000370989; ENSG00000120217 [Q9NZQ7-1]
GeneIDi29126
KEGGihsa:29126
UCSCiuc003zje.4 human [Q9NZQ7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPD1L1_HUMAN
AccessioniPrimary (citable) accession number: Q9NZQ7
Secondary accession number(s): B2RBA2
, B4DU27, Q14CJ2, Q2V8D5, Q66RK1, Q6WEX4, Q9NUZ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: October 1, 2000
Last modified: June 20, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

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