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Protein

DnaJ homolog subfamily C member 27

Gene

DNAJC27

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase which can activate the MEK/ERK pathway and induce cell transformation when overexpressed. May act as a nuclear scaffold for MAPK1, probably by association with MAPK1 nuclear export signal leading to enhanced ERK1/ERK2 signaling.By similarity

Miscellaneous

DNAJC27/RBJ knockdown in several colorectal cancer cell lines is correlated to inhibition of MEK/ERK activation, cell proliferation, colony formation and in vivo tumor growth.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi23 – 30GTPBy similarity8
Nucleotide bindingi71 – 75GTPBy similarity5
Nucleotide bindingi134 – 137GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily C member 27
Alternative name(s):
Rab and DnaJ domain-containing protein
Gene namesi
Name:DNAJC27
Synonyms:RABJS, RBJ
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000115137.11
HGNCiHGNC:30290 DNAJC27
MIMi613527 gene
neXtProtiNX_Q9NZQ0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Oncogene

Organism-specific databases

DisGeNETi51277
OpenTargetsiENSG00000115137
PharmGKBiPA164718860

Polymorphism and mutation databases

BioMutaiDNAJC27
DMDMi74734733

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003329751 – 273DnaJ homolog subfamily C member 27Add BLAST273

Proteomic databases

PaxDbiQ9NZQ0
PeptideAtlasiQ9NZQ0
PRIDEiQ9NZQ0
ProteomicsDBi83475
83476 [Q9NZQ0-2]
83477 [Q9NZQ0-3]

PTM databases

iPTMnetiQ9NZQ0
PhosphoSitePlusiQ9NZQ0

Expressioni

Tissue specificityi

Overexpressed in gastrointestinal cancers; expression correlates with later tumor-node-metastasis stages of colorectal cancers.1 Publication

Gene expression databases

BgeeiENSG00000115137
CleanExiHS_DNAJC27
GenevisibleiQ9NZQ0 HS

Organism-specific databases

HPAiHPA036815
HPA036816

Interactioni

Subunit structurei

Interacts directly with MAPK1 (wild-type and kinase-deficient forms). Interacts directly (in GTP-bound form) with MAP2K1 (wild-type and kinase-deficient forms).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
TFCP2Q128005EBI-10317544,EBI-717422

Protein-protein interaction databases

BioGridi119429, 6 interactors
IntActiQ9NZQ0, 1 interactor
STRINGi9606.ENSP00000264711

Structurei

Secondary structure

1273
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi203 – 213Combined sources11
Helixi218 – 222Combined sources5
Helixi230 – 244Combined sources15
Turni246 – 248Combined sources3
Helixi254 – 272Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YS8NMR-A197-273[»]
ProteinModelPortaliQ9NZQ0
SMRiQ9NZQ0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NZQ0

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini217 – 273JPROSITE-ProRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 18Required for interaction with MAPK1By similarityAdd BLAST18

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0098 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000119101
HOGENOMiHOG000233969
HOVERGENiHBG056504
InParanoidiQ9NZQ0
KOiK19372
OMAiFYVSIVD
OrthoDBiEOG091G0IXD
PhylomeDBiQ9NZQ0
TreeFamiTF328564

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
InterProiView protein in InterPro
IPR001623 DnaJ_domain
IPR036869 J_dom_sf
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF00071 Ras, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS50076 DNAJ_2, 1 hit
PS51419 RAB, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NZQ0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEANMPKRKE PGRSLRIKVI SMGNAEVGKS CIIKRYCEKR FVSKYLATIG
60 70 80 90 100
IDYGVTKVHV RDREIKVNIF DMAGHPFFYE VRNEFYKDTQ GVILVYDVGQ
110 120 130 140 150
KDSFDALDAW LAEMKQELGP HGNMENIIFV VCANKIDCTK HRCVDESEGR
160 170 180 190 200
LWAESKGFLY FETSAQTGEG INEMFQTFYI SIVDLCENGG KRPTTNSSAS
210 220 230 240 250
FTKEQADAIR RIRNSKDSWD MLGVKPGASR DEVNKAYRKL AVLLHPDKCV
260 270
APGSEDAFKA VVNARTALLK NIK
Length:273
Mass (Da):30,855
Last modified:October 1, 2000 - v1
Checksum:iA3A0CE43EA6A2BCA
GO
Isoform 2 (identifier: Q9NZQ0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     81-90: VRNEFYKDTQ → MRKLRRREVK
     91-273: Missing.

Show »
Length:90
Mass (Da):10,622
Checksum:i24A41685FE2A3C94
GO
Isoform 3 (identifier: Q9NZQ0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     177-177: T → G
     178-273: Missing.

Show »
Length:177
Mass (Da):20,264
Checksum:iEECCC11FF0F5130B
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03340981 – 90VRNEFYKDTQ → MRKLRRREVK in isoform 2. 1 Publication10
Alternative sequenceiVSP_03341091 – 273Missing in isoform 2. 1 PublicationAdd BLAST183
Alternative sequenceiVSP_033411177T → G in isoform 3. 1 Publication1
Alternative sequenceiVSP_033412178 – 273Missing in isoform 3. 1 PublicationAdd BLAST96

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF178983 mRNA Translation: AAF44347.1
AY094594 mRNA Translation: AAM22521.1
AL137731 mRNA Translation: CAI46214.1
CH471053 Genomic DNA Translation: EAX00736.1
CH471053 Genomic DNA Translation: EAX00735.1
CH471053 Genomic DNA Translation: EAX00737.1
BC034049 mRNA Translation: AAH34049.1
BK001284 mRNA Translation: DAA01323.1
CCDSiCCDS1716.1 [Q9NZQ0-1]
CCDS74493.1 [Q9NZQ0-3]
RefSeqiNP_001185488.1, NM_001198559.1 [Q9NZQ0-3]
NP_057628.1, NM_016544.2 [Q9NZQ0-1]
UniGeneiHs.434993

Genome annotation databases

EnsembliENST00000264711; ENSP00000264711; ENSG00000115137 [Q9NZQ0-1]
ENST00000380809; ENSP00000370187; ENSG00000115137 [Q9NZQ0-2]
ENST00000534855; ENSP00000440086; ENSG00000115137 [Q9NZQ0-3]
GeneIDi51277
KEGGihsa:51277
UCSCiuc002rft.2 human [Q9NZQ0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDJC27_HUMAN
AccessioniPrimary (citable) accession number: Q9NZQ0
Secondary accession number(s): Q5JV88, Q86Y24
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 1, 2000
Last modified: June 20, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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