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Protein

CCR4-NOT transcription complex subunit 2

Gene

CNOT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for the CCR4-NOT complex structural integrity. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may specifically involve the N-Cor repressor complex containing HDAC3, NCOR1 and NCOR2. Involved in the maintenance of emryonic stem (ES) cell identity.4 Publications

GO - Molecular functioni

  • RNA polymerase II transcription corepressor binding Source: UniProtKB
  • transcription coregulator activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Repressor
Biological processRNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation

Enzyme and pathway databases

ReactomeiR-HSA-429947 Deadenylation of mRNA
R-HSA-6804115 TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain

Names & Taxonomyi

Protein namesi
Recommended name:
CCR4-NOT transcription complex subunit 2
Alternative name(s):
CCR4-associated factor 2
Gene namesi
Name:CNOT2
Synonyms:CDC36, NOT2
ORF Names:HSPC131, MSTP046
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000111596.11
HGNCiHGNC:7878 CNOT2
MIMi604909 gene
neXtProtiNX_Q9NZN8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4848
OpenTargetsiENSG00000111596
PharmGKBiPA26673

Polymorphism and mutation databases

BioMutaiCNOT2
DMDMi46396017

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001983311 – 540CCR4-NOT transcription complex subunit 2Add BLAST540

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei93PhosphothreonineCombined sources1
Modified residuei126PhosphoserineCombined sources1
Modified residuei157PhosphoserineBy similarity1
Modified residuei165PhosphoserineCombined sources1
Modified residuei169PhosphoserineBy similarity1
Modified residuei242PhosphoserineCombined sources1
Modified residuei274PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9NZN8
MaxQBiQ9NZN8
PaxDbiQ9NZN8
PeptideAtlasiQ9NZN8
PRIDEiQ9NZN8
ProteomicsDBi83462
83463 [Q9NZN8-2]
83464 [Q9NZN8-3]
83465 [Q9NZN8-4]
83466 [Q9NZN8-5]

PTM databases

iPTMnetiQ9NZN8
PhosphoSitePlusiQ9NZN8

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocytes.1 Publication

Developmental stagei

Expressed in embryonic stem (ES) cells.1 Publication

Gene expression databases

BgeeiENSG00000111596 Expressed in 241 organ(s), highest expression level in intestine
CleanExiHS_CNOT2
ExpressionAtlasiQ9NZN8 baseline and differential
GenevisibleiQ9NZN8 HS

Organism-specific databases

HPAiHPA067711

Interactioni

Subunit structurei

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. In the complex interacts directly with CNOT3. Interacts with NCOR1, NCOR2. HDAC3 and GPS2.4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110910, 64 interactors
CORUMiQ9NZN8
DIPiDIP-42656N
IntActiQ9NZN8, 62 interactors
MINTiQ9NZN8
STRINGi9606.ENSP00000229195

Structurei

Secondary structure

1540
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9NZN8
SMRiQ9NZN8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni437 – 540Repressor domainAdd BLAST104

Sequence similaritiesi

Belongs to the CNOT2/3/5 family.Curated

Phylogenomic databases

eggNOGiKOG2151 Eukaryota
COG5601 LUCA
GeneTreeiENSGT00390000001285
HOGENOMiHOG000246523
HOVERGENiHBG051041
InParanoidiQ9NZN8
KOiK12605
OMAiLKTNTYE
OrthoDBiEOG091G0C7Y
PhylomeDBiQ9NZN8
TreeFamiTF313102

Family and domain databases

Gene3Di2.30.30.1020, 1 hit
InterProiView protein in InterPro
IPR038635 CCR4-NOT_su2/3/5_N_sf
IPR007282 NOT2/3/5_C
PfamiView protein in Pfam
PF04153 NOT2_3_5, 1 hit

Sequences (5+)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 5 described isoforms and 22 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9NZN8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVRTDGHTLS EKRNYQVTNS MFGASRKKFV EGVDSDYHDE NMYYSQSSMF
60 70 80 90 100
PHRSEKDMLA SPSTSGQLSQ FGASLYGQQS ALGLPMRGMS NNTPQLNRSL
110 120 130 140 150
SQGTQLPSHV TPTTGVPTMS LHTPPSPSRG ILPMNPRNMM NHSQVGQGIG
160 170 180 190 200
IPSRTNSMSS SGLGSPNRSS PSIICMPKQQ PSRQPFTVNS MSGFGMNRNQ
210 220 230 240 250
AFGMNNSLSS NIFNGTDGSE NVTGLDLSDF PALADRNRRE GSGNPTPLIN
260 270 280 290 300
PLAGRAPYVG MVTKPANEQS QDFSIHNEDF PALPGSSYKD PTSSNDDSKS
310 320 330 340 350
NLNTSGKTTS STDGPKFPGD KSSTTQNNNQ QKKGIQVLPD GRVTNIPQGM
360 370 380 390 400
VTDQFGMIGL LTFIRAAETD PGMVHLALGS DLTTLGLNLN SPENLYPKFA
410 420 430 440 450
SPWASSPCRP QDIDFHVPSE YLTNIHIRDK LAAIKLGRYG EDLLFYLYYM
460 470 480 490 500
NGGDVLQLLA AVELFNRDWR YHKEERVWIT RAPGMEPTMK TNTYERGTYY
510 520 530 540
FFDCLNWRKV AKEFHLEYDK LEERPHLPST FNYNPAQQAF
Length:540
Mass (Da):59,738
Last modified:October 1, 2000 - v1
Checksum:i7183890F033E3B83
GO
Isoform 2 (identifier: Q9NZN8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-175: Missing.

Note: No experimental confirmation available.
Show »
Length:365
Mass (Da):40,818
Checksum:i27FC908ADA1A6201
GO
Isoform 3 (identifier: Q9NZN8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-349: Missing.

Note: May be due to an intron retention. No experimental confirmation available.
Show »
Length:191
Mass (Da):22,254
Checksum:i803037A4A6F670CC
GO
Isoform 4 (identifier: Q9NZN8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     465-475: FNRDWRYHKEE → YVQSILITFVL
     476-540: Missing.

Note: May be due to an intron retention. No experimental confirmation available.
Show »
Length:475
Mass (Da):51,537
Checksum:i5D5C630BB3E89F06
GO
Isoform 5 (identifier: Q9NZN8-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     229-278: Missing.

Note: No experimental confirmation available.
Show »
Length:490
Mass (Da):54,306
Checksum:i7C6A4CC0F0992C9C
GO

Computationally mapped potential isoform sequencesi

There are 22 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8VV52F8VV52_HUMAN
CCR4-NOT transcription complex subu...
CNOT2
531Annotation score:
F8VRS8F8VRS8_HUMAN
CCR4-NOT transcription complex subu...
CNOT2
276Annotation score:
F8VP97F8VP97_HUMAN
CCR4-NOT transcription complex subu...
CNOT2
321Annotation score:
F8VQF4F8VQF4_HUMAN
CCR4-NOT transcription complex subu...
CNOT2
144Annotation score:
F8W145F8W145_HUMAN
CCR4-NOT transcription complex subu...
CNOT2
309Annotation score:
F8VUB4F8VUB4_HUMAN
CCR4-NOT transcription complex subu...
CNOT2
520Annotation score:
H0YH84H0YH84_HUMAN
CCR4-NOT transcription complex subu...
CNOT2
186Annotation score:
H0YHT6H0YHT6_HUMAN
CCR4-NOT transcription complex subu...
CNOT2
104Annotation score:
H0YI11H0YI11_HUMAN
CCR4-NOT transcription complex subu...
CNOT2
182Annotation score:
H0YI80H0YI80_HUMAN
CCR4-NOT transcription complex subu...
CNOT2
99Annotation score:
There are more potential isoformsShow all

Sequence cautioni

The sequence AAF29095 differs from that shown. Reason: Frameshift at position 474.Curated
The sequence AAQ13426 differs from that shown. Reason: Frameshift at positions 71, 76, 84, 98, 123, 370, 384 and 393.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048750460A → T. Corresponds to variant dbSNP:rs11178192Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0099131 – 349Missing in isoform 3. 1 PublicationAdd BLAST349
Alternative sequenceiVSP_0099121 – 175Missing in isoform 2. 1 PublicationAdd BLAST175
Alternative sequenceiVSP_009914229 – 278Missing in isoform 5. 1 PublicationAdd BLAST50
Alternative sequenceiVSP_009915465 – 475FNRDWRYHKEE → YVQSILITFVL in isoform 4. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_009916476 – 540Missing in isoform 4. 1 PublicationAdd BLAST65

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF180473 mRNA Translation: AAF29827.1
AF113226 mRNA Translation: AAG39297.1
AF161480 mRNA Translation: AAF29095.1 Frameshift.
AK000662 mRNA Translation: BAA91313.1
AL137674 mRNA Translation: CAB70869.1
BX641116 mRNA Translation: CAE46054.1
BC002597 mRNA Translation: AAH02597.1
BC011826 mRNA Translation: AAH11826.1
AF044215 mRNA Translation: AAQ13426.1 Frameshift.
CCDSiCCDS31857.1 [Q9NZN8-1]
PIRiT46494
RefSeqiNP_001186231.1, NM_001199302.1 [Q9NZN8-1]
NP_001186232.1, NM_001199303.1 [Q9NZN8-1]
NP_055330.1, NM_014515.5 [Q9NZN8-1]
XP_011536701.1, XM_011538399.1 [Q9NZN8-1]
UniGeneiHs.133350
Hs.730666

Genome annotation databases

EnsembliENST00000229195; ENSP00000229195; ENSG00000111596 [Q9NZN8-1]
ENST00000418359; ENSP00000412091; ENSG00000111596 [Q9NZN8-1]
ENST00000551043; ENSP00000449260; ENSG00000111596 [Q9NZN8-1]
ENST00000551483; ENSP00000448883; ENSG00000111596 [Q9NZN8-3]
GeneIDi4848
KEGGihsa:4848
UCSCiuc001svv.4 human [Q9NZN8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF180473 mRNA Translation: AAF29827.1
AF113226 mRNA Translation: AAG39297.1
AF161480 mRNA Translation: AAF29095.1 Frameshift.
AK000662 mRNA Translation: BAA91313.1
AL137674 mRNA Translation: CAB70869.1
BX641116 mRNA Translation: CAE46054.1
BC002597 mRNA Translation: AAH02597.1
BC011826 mRNA Translation: AAH11826.1
AF044215 mRNA Translation: AAQ13426.1 Frameshift.
CCDSiCCDS31857.1 [Q9NZN8-1]
PIRiT46494
RefSeqiNP_001186231.1, NM_001199302.1 [Q9NZN8-1]
NP_001186232.1, NM_001199303.1 [Q9NZN8-1]
NP_055330.1, NM_014515.5 [Q9NZN8-1]
XP_011536701.1, XM_011538399.1 [Q9NZN8-1]
UniGeneiHs.133350
Hs.730666

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4C0DX-ray3.20B344-540[»]
4C0FX-ray2.40A/B/C/D429-540[»]
5FU6X-ray2.90B/E350-540[»]
5FU7X-ray3.10B/F350-540[»]
ProteinModelPortaliQ9NZN8
SMRiQ9NZN8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110910, 64 interactors
CORUMiQ9NZN8
DIPiDIP-42656N
IntActiQ9NZN8, 62 interactors
MINTiQ9NZN8
STRINGi9606.ENSP00000229195

PTM databases

iPTMnetiQ9NZN8
PhosphoSitePlusiQ9NZN8

Polymorphism and mutation databases

BioMutaiCNOT2
DMDMi46396017

Proteomic databases

EPDiQ9NZN8
MaxQBiQ9NZN8
PaxDbiQ9NZN8
PeptideAtlasiQ9NZN8
PRIDEiQ9NZN8
ProteomicsDBi83462
83463 [Q9NZN8-2]
83464 [Q9NZN8-3]
83465 [Q9NZN8-4]
83466 [Q9NZN8-5]

Protocols and materials databases

DNASUi4848
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000229195; ENSP00000229195; ENSG00000111596 [Q9NZN8-1]
ENST00000418359; ENSP00000412091; ENSG00000111596 [Q9NZN8-1]
ENST00000551043; ENSP00000449260; ENSG00000111596 [Q9NZN8-1]
ENST00000551483; ENSP00000448883; ENSG00000111596 [Q9NZN8-3]
GeneIDi4848
KEGGihsa:4848
UCSCiuc001svv.4 human [Q9NZN8-1]

Organism-specific databases

CTDi4848
DisGeNETi4848
EuPathDBiHostDB:ENSG00000111596.11
GeneCardsiCNOT2
HGNCiHGNC:7878 CNOT2
HPAiHPA067711
MIMi604909 gene
neXtProtiNX_Q9NZN8
OpenTargetsiENSG00000111596
PharmGKBiPA26673
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2151 Eukaryota
COG5601 LUCA
GeneTreeiENSGT00390000001285
HOGENOMiHOG000246523
HOVERGENiHBG051041
InParanoidiQ9NZN8
KOiK12605
OMAiLKTNTYE
OrthoDBiEOG091G0C7Y
PhylomeDBiQ9NZN8
TreeFamiTF313102

Enzyme and pathway databases

ReactomeiR-HSA-429947 Deadenylation of mRNA
R-HSA-6804115 TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain

Miscellaneous databases

ChiTaRSiCNOT2 human
GeneWikiiCNOT2
GenomeRNAii4848
PROiPR:Q9NZN8
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000111596 Expressed in 241 organ(s), highest expression level in intestine
CleanExiHS_CNOT2
ExpressionAtlasiQ9NZN8 baseline and differential
GenevisibleiQ9NZN8 HS

Family and domain databases

Gene3Di2.30.30.1020, 1 hit
InterProiView protein in InterPro
IPR038635 CCR4-NOT_su2/3/5_N_sf
IPR007282 NOT2/3/5_C
PfamiView protein in Pfam
PF04153 NOT2_3_5, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCNOT2_HUMAN
AccessioniPrimary (citable) accession number: Q9NZN8
Secondary accession number(s): Q9H3E0
, Q9NSX5, Q9NWR6, Q9P028
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: October 1, 2000
Last modified: November 7, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
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