UniProtKB - Q9NZN8 (CNOT2_HUMAN)
CCR4-NOT transcription complex subunit 2
CNOT2
Functioni
Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for the CCR4-NOT complex structural integrity. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may specifically involve the N-Cor repressor complex containing HDAC3, NCOR1 and NCOR2. Involved in the maintenance of embryonic stem (ES) cell identity.
4 PublicationsGO - Molecular functioni
- transcription coregulator activity Source: UniProtKB
- transcription corepressor binding Source: UniProtKB
GO - Biological processi
- gene silencing by RNA Source: UniProtKB-KW
- negative regulation of intracellular estrogen receptor signaling pathway Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- nuclear-transcribed mRNA poly(A) tail shortening Source: GO_Central
- positive regulation of cytoplasmic mRNA processing body assembly Source: UniProtKB
- regulation of stem cell population maintenance Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: UniProtKB
- RNA phosphodiester bond hydrolysis, exonucleolytic Source: GOC
- trophectodermal cell differentiation Source: Ensembl
Keywordsi
Molecular function | Developmental protein, Repressor |
Biological process | RNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation |
Enzyme and pathway databases
PathwayCommonsi | Q9NZN8 |
Reactomei | R-HSA-429947, Deadenylation of mRNA R-HSA-6804115, TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain |
SignaLinki | Q9NZN8 |
Names & Taxonomyi
Protein namesi | Recommended name: CCR4-NOT transcription complex subunit 2Alternative name(s): CCR4-associated factor 2 |
Gene namesi | Name:CNOT2 Synonyms:CDC36, NOT2 ORF Names:HSPC131, MSTP046 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7878, CNOT2 |
MIMi | 604909, gene |
neXtProti | NX_Q9NZN8 |
VEuPathDBi | HostDB:ENSG00000111596 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: HPA
Other locations
- CCR4-NOT complex Source: UniProtKB
- CCR4-NOT core complex Source: GO_Central
- cytoplasm Source: UniProtKB
- membrane Source: UniProtKB
- P-body Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Involvement in diseasei
Intellectual developmental disorder with nasal speech, dysmorphic facies, and variable skeletal anomalies (IDNADFS)2 Publications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_083417 | 316 – 540 | Missing in IDNADFS. 1 PublicationAdd BLAST | 225 |
Keywords - Diseasei
Disease variant, Mental retardationOrganism-specific databases
DisGeNETi | 4848 |
MalaCardsi | CNOT2 |
MIMi | 618608, phenotype |
OpenTargetsi | ENSG00000111596 |
Orphaneti | 289513, 12q15q21.1 microdeletion syndrome |
PharmGKBi | PA26673 |
Miscellaneous databases
Pharosi | Q9NZN8, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4105920 |
Genetic variation databases
BioMutai | CNOT2 |
DMDMi | 46396017 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000198331 | 1 – 540 | CCR4-NOT transcription complex subunit 2Add BLAST | 540 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 93 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 126 | PhosphoserineCombined sources | 1 | |
Modified residuei | 157 | PhosphoserineBy similarity | 1 | |
Modified residuei | 165 | PhosphoserineCombined sources | 1 | |
Modified residuei | 169 | PhosphoserineBy similarity | 1 | |
Modified residuei | 242 | PhosphoserineCombined sources | 1 | |
Modified residuei | 274 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9NZN8 |
jPOSTi | Q9NZN8 |
MassIVEi | Q9NZN8 |
MaxQBi | Q9NZN8 |
PaxDbi | Q9NZN8 |
PeptideAtlasi | Q9NZN8 |
PRIDEi | Q9NZN8 |
ProteomicsDBi | 83462 [Q9NZN8-1] 83463 [Q9NZN8-2] 83464 [Q9NZN8-3] 83465 [Q9NZN8-4] 83466 [Q9NZN8-5] |
PTM databases
GlyGeni | Q9NZN8, 2 sites, 1 O-linked glycan (2 sites) |
iPTMneti | Q9NZN8 |
MetOSitei | Q9NZN8 |
PhosphoSitePlusi | Q9NZN8 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSG00000111596, Expressed in intestine and 253 other tissues |
ExpressionAtlasi | Q9NZN8, baseline and differential |
Genevisiblei | Q9NZN8, HS |
Organism-specific databases
HPAi | ENSG00000111596, Low tissue specificity |
Interactioni
Subunit structurei
Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. In the complex interacts directly with CNOT3.
Interacts with NCOR1, NCOR2. HDAC3 and GPS2.
4 PublicationsBinary interactionsi
Q9NZN8
GO - Molecular functioni
- transcription corepressor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 110910, 164 interactors |
ComplexPortali | CPX-2522, CCR4-NOT mRNA deadenylase complex, CNOT6L-CNOT7 variant CPX-2535, CCR4-NOT mRNA deadenylase complex, CNOT6L-CNOT8 variant CPX-2849, CCR4-NOT mRNA deadenylase complex, CNOT6-CNOT8 variant CPX-707, CCR4-NOT mRNA deadenylase complex, CNOT6-CNOT7 variant |
CORUMi | Q9NZN8 |
DIPi | DIP-42656N |
IntActi | Q9NZN8, 88 interactors |
MINTi | Q9NZN8 |
STRINGi | 9606.ENSP00000229195 |
Miscellaneous databases
RNActi | Q9NZN8, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9NZN8 |
SMRi | Q9NZN8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 96 – 126 | DisorderedSequence analysisAdd BLAST | 31 | |
Regioni | 276 – 342 | DisorderedSequence analysisAdd BLAST | 67 | |
Regioni | 437 – 540 | Repressor domainAdd BLAST | 104 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 96 – 125 | Polar residuesSequence analysisAdd BLAST | 30 | |
Compositional biasi | 287 – 335 | Polar residuesSequence analysisAdd BLAST | 49 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2151, Eukaryota |
GeneTreei | ENSGT00390000001285 |
HOGENOMi | CLU_033275_1_0_1 |
InParanoidi | Q9NZN8 |
OMAi | GMNRNQV |
PhylomeDBi | Q9NZN8 |
TreeFami | TF313102 |
Family and domain databases
Gene3Di | 2.30.30.1020, 1 hit |
IDEALi | IID00503 |
InterProi | View protein in InterPro IPR038635, CCR4-NOT_su2/3/5_N_sf IPR040168, Not2/3/5 IPR007282, NOT2/3/5_C |
PANTHERi | PTHR23326, PTHR23326, 1 hit |
Pfami | View protein in Pfam PF04153, NOT2_3_5, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 21 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVRTDGHTLS EKRNYQVTNS MFGASRKKFV EGVDSDYHDE NMYYSQSSMF
60 70 80 90 100
PHRSEKDMLA SPSTSGQLSQ FGASLYGQQS ALGLPMRGMS NNTPQLNRSL
110 120 130 140 150
SQGTQLPSHV TPTTGVPTMS LHTPPSPSRG ILPMNPRNMM NHSQVGQGIG
160 170 180 190 200
IPSRTNSMSS SGLGSPNRSS PSIICMPKQQ PSRQPFTVNS MSGFGMNRNQ
210 220 230 240 250
AFGMNNSLSS NIFNGTDGSE NVTGLDLSDF PALADRNRRE GSGNPTPLIN
260 270 280 290 300
PLAGRAPYVG MVTKPANEQS QDFSIHNEDF PALPGSSYKD PTSSNDDSKS
310 320 330 340 350
NLNTSGKTTS STDGPKFPGD KSSTTQNNNQ QKKGIQVLPD GRVTNIPQGM
360 370 380 390 400
VTDQFGMIGL LTFIRAAETD PGMVHLALGS DLTTLGLNLN SPENLYPKFA
410 420 430 440 450
SPWASSPCRP QDIDFHVPSE YLTNIHIRDK LAAIKLGRYG EDLLFYLYYM
460 470 480 490 500
NGGDVLQLLA AVELFNRDWR YHKEERVWIT RAPGMEPTMK TNTYERGTYY
510 520 530 540
FFDCLNWRKV AKEFHLEYDK LEERPHLPST FNYNPAQQAF
Computationally mapped potential isoform sequencesi
There are 21 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8VV52 | F8VV52_HUMAN | CCR4-NOT transcription complex subu... | CNOT2 | 531 | Annotation score: | ||
F8VUB4 | F8VUB4_HUMAN | CCR4-NOT transcription complex subu... | CNOT2 | 520 | Annotation score: | ||
F8VRS8 | F8VRS8_HUMAN | CCR4-NOT transcription complex subu... | CNOT2 | 276 | Annotation score: | ||
F8W145 | F8W145_HUMAN | CCR4-NOT transcription complex subu... | CNOT2 | 309 | Annotation score: | ||
F8VQF4 | F8VQF4_HUMAN | CCR4-NOT transcription complex subu... | CNOT2 | 144 | Annotation score: | ||
H0YH84 | H0YH84_HUMAN | CCR4-NOT transcription complex subu... | CNOT2 | 186 | Annotation score: | ||
H0YI11 | H0YI11_HUMAN | CCR4-NOT transcription complex subu... | CNOT2 | 182 | Annotation score: | ||
F8VQD8 | F8VQD8_HUMAN | CCR4-NOT transcription complex subu... | CNOT2 | 131 | Annotation score: | ||
F8VX63 | F8VX63_HUMAN | CCR4-NOT transcription complex subu... | CNOT2 | 217 | Annotation score: | ||
F8VVY1 | F8VVY1_HUMAN | CCR4-NOT transcription complex subu... | CNOT2 | 182 | Annotation score: | ||
There are more potential isoformsShow all |
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_083417 | 316 – 540 | Missing in IDNADFS. 1 PublicationAdd BLAST | 225 | |
Natural variantiVAR_048750 | 460 | A → T. Corresponds to variant dbSNP:rs11178192Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009913 | 1 – 349 | Missing in isoform 3. 1 PublicationAdd BLAST | 349 | |
Alternative sequenceiVSP_009912 | 1 – 175 | Missing in isoform 2. 1 PublicationAdd BLAST | 175 | |
Alternative sequenceiVSP_009914 | 229 – 278 | Missing in isoform 5. 1 PublicationAdd BLAST | 50 | |
Alternative sequenceiVSP_009915 | 465 – 475 | FNRDWRYHKEE → YVQSILITFVL in isoform 4. 1 PublicationAdd BLAST | 11 | |
Alternative sequenceiVSP_009916 | 476 – 540 | Missing in isoform 4. 1 PublicationAdd BLAST | 65 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF180473 mRNA Translation: AAF29827.1 AF113226 mRNA Translation: AAG39297.1 AF161480 mRNA Translation: AAF29095.1 Frameshift. AK000662 mRNA Translation: BAA91313.1 AL137674 mRNA Translation: CAB70869.1 BX641116 mRNA Translation: CAE46054.1 BC002597 mRNA Translation: AAH02597.1 BC011826 mRNA Translation: AAH11826.1 AF044215 mRNA Translation: AAQ13426.1 Frameshift. |
CCDSi | CCDS31857.1 [Q9NZN8-1] |
PIRi | T46494 |
RefSeqi | NP_001186231.1, NM_001199302.1 [Q9NZN8-1] NP_001186232.1, NM_001199303.1 [Q9NZN8-1] NP_055330.1, NM_014515.5 [Q9NZN8-1] XP_011536701.1, XM_011538399.1 [Q9NZN8-1] |
Genome annotation databases
Ensembli | ENST00000229195.8; ENSP00000229195.3; ENSG00000111596.14 ENST00000418359.7; ENSP00000412091.3; ENSG00000111596.14 ENST00000551043.5; ENSP00000449260.1; ENSG00000111596.14 ENST00000551483.5; ENSP00000448883.1; ENSG00000111596.14 [Q9NZN8-3] |
GeneIDi | 4848 |
KEGGi | hsa:4848 |
MANE-Selecti | ENST00000229195.8; ENSP00000229195.3; NM_014515.7; NP_055330.1 |
UCSCi | uc001svv.4, human [Q9NZN8-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF180473 mRNA Translation: AAF29827.1 AF113226 mRNA Translation: AAG39297.1 AF161480 mRNA Translation: AAF29095.1 Frameshift. AK000662 mRNA Translation: BAA91313.1 AL137674 mRNA Translation: CAB70869.1 BX641116 mRNA Translation: CAE46054.1 BC002597 mRNA Translation: AAH02597.1 BC011826 mRNA Translation: AAH11826.1 AF044215 mRNA Translation: AAQ13426.1 Frameshift. |
CCDSi | CCDS31857.1 [Q9NZN8-1] |
PIRi | T46494 |
RefSeqi | NP_001186231.1, NM_001199302.1 [Q9NZN8-1] NP_001186232.1, NM_001199303.1 [Q9NZN8-1] NP_055330.1, NM_014515.5 [Q9NZN8-1] XP_011536701.1, XM_011538399.1 [Q9NZN8-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4C0D | X-ray | 3.20 | B | 344-540 | [»] | |
4C0F | X-ray | 2.40 | A/B/C/D | 429-540 | [»] | |
5FU6 | X-ray | 2.90 | B/E | 350-540 | [»] | |
5FU7 | X-ray | 3.10 | B/F | 350-540 | [»] | |
AlphaFoldDBi | Q9NZN8 | |||||
SMRi | Q9NZN8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110910, 164 interactors |
ComplexPortali | CPX-2522, CCR4-NOT mRNA deadenylase complex, CNOT6L-CNOT7 variant CPX-2535, CCR4-NOT mRNA deadenylase complex, CNOT6L-CNOT8 variant CPX-2849, CCR4-NOT mRNA deadenylase complex, CNOT6-CNOT8 variant CPX-707, CCR4-NOT mRNA deadenylase complex, CNOT6-CNOT7 variant |
CORUMi | Q9NZN8 |
DIPi | DIP-42656N |
IntActi | Q9NZN8, 88 interactors |
MINTi | Q9NZN8 |
STRINGi | 9606.ENSP00000229195 |
Chemistry databases
ChEMBLi | CHEMBL4105920 |
PTM databases
GlyGeni | Q9NZN8, 2 sites, 1 O-linked glycan (2 sites) |
iPTMneti | Q9NZN8 |
MetOSitei | Q9NZN8 |
PhosphoSitePlusi | Q9NZN8 |
Genetic variation databases
BioMutai | CNOT2 |
DMDMi | 46396017 |
Proteomic databases
EPDi | Q9NZN8 |
jPOSTi | Q9NZN8 |
MassIVEi | Q9NZN8 |
MaxQBi | Q9NZN8 |
PaxDbi | Q9NZN8 |
PeptideAtlasi | Q9NZN8 |
PRIDEi | Q9NZN8 |
ProteomicsDBi | 83462 [Q9NZN8-1] 83463 [Q9NZN8-2] 83464 [Q9NZN8-3] 83465 [Q9NZN8-4] 83466 [Q9NZN8-5] |
Protocols and materials databases
Antibodypediai | 17029, 440 antibodies from 33 providers |
DNASUi | 4848 |
Genome annotation databases
Ensembli | ENST00000229195.8; ENSP00000229195.3; ENSG00000111596.14 ENST00000418359.7; ENSP00000412091.3; ENSG00000111596.14 ENST00000551043.5; ENSP00000449260.1; ENSG00000111596.14 ENST00000551483.5; ENSP00000448883.1; ENSG00000111596.14 [Q9NZN8-3] |
GeneIDi | 4848 |
KEGGi | hsa:4848 |
MANE-Selecti | ENST00000229195.8; ENSP00000229195.3; NM_014515.7; NP_055330.1 |
UCSCi | uc001svv.4, human [Q9NZN8-1] |
Organism-specific databases
CTDi | 4848 |
DisGeNETi | 4848 |
GeneCardsi | CNOT2 |
HGNCi | HGNC:7878, CNOT2 |
HPAi | ENSG00000111596, Low tissue specificity |
MalaCardsi | CNOT2 |
MIMi | 604909, gene 618608, phenotype |
neXtProti | NX_Q9NZN8 |
OpenTargetsi | ENSG00000111596 |
Orphaneti | 289513, 12q15q21.1 microdeletion syndrome |
PharmGKBi | PA26673 |
VEuPathDBi | HostDB:ENSG00000111596 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2151, Eukaryota |
GeneTreei | ENSGT00390000001285 |
HOGENOMi | CLU_033275_1_0_1 |
InParanoidi | Q9NZN8 |
OMAi | GMNRNQV |
PhylomeDBi | Q9NZN8 |
TreeFami | TF313102 |
Enzyme and pathway databases
PathwayCommonsi | Q9NZN8 |
Reactomei | R-HSA-429947, Deadenylation of mRNA R-HSA-6804115, TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain |
SignaLinki | Q9NZN8 |
Miscellaneous databases
BioGRID-ORCSi | 4848, 295 hits in 1086 CRISPR screens |
ChiTaRSi | CNOT2, human |
GeneWikii | CNOT2 |
GenomeRNAii | 4848 |
Pharosi | Q9NZN8, Tbio |
PROi | PR:Q9NZN8 |
RNActi | Q9NZN8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000111596, Expressed in intestine and 253 other tissues |
ExpressionAtlasi | Q9NZN8, baseline and differential |
Genevisiblei | Q9NZN8, HS |
Family and domain databases
Gene3Di | 2.30.30.1020, 1 hit |
IDEALi | IID00503 |
InterProi | View protein in InterPro IPR038635, CCR4-NOT_su2/3/5_N_sf IPR040168, Not2/3/5 IPR007282, NOT2/3/5_C |
PANTHERi | PTHR23326, PTHR23326, 1 hit |
Pfami | View protein in Pfam PF04153, NOT2_3_5, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CNOT2_HUMAN | |
Accessioni | Q9NZN8Primary (citable) accession number: Q9NZN8 Secondary accession number(s): Q9H3E0 Q9P028 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2004 |
Last sequence update: | October 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 182 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families