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Protein

Ribosome biogenesis protein NOP53

Gene

NOP53

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Nucleolar protein which is involved in the integration of the 5S RNP into the ribosomal large subunit during ribosome biogenesis (PubMed:24120868). In ribosome biogenesis, may also play a role in rRNA transcription (PubMed:27729611). Also functions as a nucleolar sensor that regulates the activation of p53/TP53 in response to ribosome biogenesis perturbation, DNA damage and other stress conditions (PubMed:21741933, PubMed:24120868, PubMed:27829214). DNA damage or perturbation of ribosome biogenesis disrupt the interaction between NOP53 and RPL11 allowing RPL11 transport to the nucleoplasm where it can inhibit MDM2 and allow p53/TP53 activation (PubMed:24120868, PubMed:27829214). It may also positively regulate the function of p53/TP53 in cell cycle arrest and apoptosis through direct interaction, preventing its MDM2-dependent ubiquitin-mediated proteasomal degradation (PubMed:22522597). Originally identified as a tumor suppressor, it may also play a role in cell proliferation and apoptosis by positively regulating the stability of PTEN, thereby antagonizing the PI3K-AKT/PKB signaling pathway (PubMed:15355975, PubMed:16971513, PubMed:27729611). May also inhibit cell proliferation and increase apoptosis through its interaction with NF2 (PubMed:21167305). May negatively regulate NPM1 by regulating its nucleoplasmic localization, oligomerization and ubiquitin-mediated proteasomal degradation (PubMed:25818168). Thereby, may prevent NPM1 interaction with MYC and negatively regulate transcription mediated by the MYC-NPM1 complex (PubMed:25956029). May also regulate cellular aerobic respiration (PubMed:24556985). In the cellular response to viral infection, may play a role in the attenuation of interferon-beta through the inhibition of DDX58/RIG-1 (PubMed:27824081).12 Publications

GO - Molecular functioni

  • 5S rRNA binding Source: HGNC
  • identical protein binding Source: UniProtKB
  • p53 binding Source: UniProtKB
  • RNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processRibosome biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome biogenesis protein NOP53Curated
Alternative name(s):
Glioma tumor suppressor candidate region gene 2 proteinImported
Protein interacting with carboxyl terminus 11 Publication
Short name:
PICT-11 Publication
p601 Publication
Gene namesi
Name:NOP53Imported
Synonyms:GLT1 Publication, GLTSCR21 Publication, PICT11 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000105373.18
HGNCiHGNC:4333 NOP53
MIMi605691 gene
neXtProtiNX_Q9NZM5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi233S → A: Decreased phosphorylation, degradation and increased interaction with RPL11 in response to DNA damage; when associated with A-289. 1 Publication1
Mutagenesisi233S → D: Loss of localization to the nucleolus and in response to DNA damage increased degradation and decreased interaction with RPL11; when associated with D-289. 1 Publication1
Mutagenesisi289T → A: Decreased degradation in response to DNA damage. Decreased phosphorylation, degradation and increased interaction with RPL11 in response to DNA damage; when associated with A-233. 1 Publication1
Mutagenesisi289T → D: Loss of localization to the nucleolus and in response to DNA damage increased degradation and decreased interaction with RPL11; when associated with D-233. 1 Publication1

Organism-specific databases

DisGeNETi29997
OpenTargetsiENSG00000105373
PharmGKBiPA28736

Polymorphism and mutation databases

BioMutaiGLTSCR2
DMDMi93141272

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002189602 – 478Ribosome biogenesis protein NOP53Add BLAST477

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei29PhosphoserineCombined sources1
Modified residuei93PhosphoserineCombined sources1
Modified residuei305PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitin-mediated proteasomal degradation is regulated by c-JUN. It is associated with relocalization to the nucleoplasm and decreased homooligomerization.1 Publication
Phosphorylated upon DNA damage probably by ATM and DNA-PK; may regulate NOP53 degradation.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9NZM5
MaxQBiQ9NZM5
PaxDbiQ9NZM5
PeptideAtlasiQ9NZM5
PRIDEiQ9NZM5
ProteomicsDBi83451

2D gel databases

SWISS-2DPAGEiQ9NZM5

PTM databases

iPTMnetiQ9NZM5
PhosphoSitePlusiQ9NZM5

Expressioni

Tissue specificityi

Expressed at high levels in heart and pancreas, moderate levels in placenta, liver, skeletal muscle, and kidney, and low levels in brain and lung.1 Publication

Inductioni

Down-regulated by nucleolar stress through ubiquitin-independent proteasomal degradation (at protein level) (PubMed:24923447). Up-regulated upon mitochondrial stress (at protein level) (PubMed:24556985). Expression of the protein might be regulated by DNA damage but results are not consistent (PubMed:21741933, PubMed:27829214).4 Publications

Gene expression databases

BgeeiENSG00000105373 Expressed in 230 organ(s), highest expression level in mammary gland
CleanExiHS_GLTSCR2
ExpressionAtlasiQ9NZM5 baseline and differential
GenevisibleiQ9NZM5 HS

Organism-specific databases

HPAiHPA018999
HPA049600

Interactioni

Subunit structurei

Homooligomer (PubMed:24735870, PubMed:26903295). Interacts with PTEN; regulates PTEN phosphorylation and increases its stability (PubMed:15355975). Interacts with RPL11; retains RPL11 into the nucleolus (PubMed:24556985, PubMed:27829214). Interacts with CDKN2A/isoform tumor suppressor ARF; the interaction is direct and promotes ARF nucleoplasmic relocalization and ubiquitin-mediated proteasomal degradation (PubMed:27323397). Interacts with NPM1; the interaction is direct and competitive with MYC (PubMed:25956029). Interacts with NF2 (via FERM domain); the interaction is direct (PubMed:21167305). Interacts with p53/TP53 (via the oligomerization region); the interaction is direct and may prevent the MDM2-mediated proteasomal degradation of p53/TP53 (PubMed:22522597). Interacts with DDX58; may regulate DDX58 through USP15-mediated 'Lys-63'-linked deubiquitination (PubMed:27824081). Interacts with UBTF (PubMed:27729611).11 Publications
(Microbial infection) Interacts with herpes simplex virus 1 early proteins ICP22 and ICP0.1 Publication
(Microbial infection) Interacts with Human herpesvirus 8 protein ORF16; may sequester ORF16 in host nucleolus and reduce its antiapoptotic activity.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
DDX58O957862EBI-720156,EBI-995350

GO - Molecular functioni

Protein-protein interaction databases

BioGridi119021, 149 interactors
IntActiQ9NZM5, 34 interactors
STRINGi9606.ENSP00000246802

Structurei

3D structure databases

ProteinModelPortaliQ9NZM5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni148 – 431Mediates interaction with CDKN2A/isoform tumor suppressor ARF1 PublicationAdd BLAST284
Regioni181 – 478Mediates interaction with NF21 PublicationAdd BLAST298
Regioni342 – 386Mediates interaction with human herpesvirus 8 protein ORF161 PublicationAdd BLAST45
Regioni347 – 395Nucleolar localization signal1 PublicationAdd BLAST49
Regioni396 – 478Nucleolar localization signal1 PublicationAdd BLAST83

Sequence similaritiesi

Belongs to the NOP53 family.Curated

Phylogenomic databases

eggNOGiKOG2823 Eukaryota
ENOG4111GSN LUCA
GeneTreeiENSGT00390000017267
HOGENOMiHOG000070127
HOVERGENiHBG051849
InParanoidiQ9NZM5
KOiK14840
OMAiKSAWRKT
OrthoDBiEOG091G0BKI
PhylomeDBiQ9NZM5
TreeFamiTF313004

Family and domain databases

InterProiView protein in InterPro
IPR011687 Nop53/GLTSCR2
PANTHERiPTHR14211 PTHR14211, 1 hit
PfamiView protein in Pfam
PF07767 Nop53, 1 hit
PIRSFiPIRSF017302 Gltscr2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

Q9NZM5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAGGSGVGG KRSSKSDADS GFLGLRPTSV DPALRRRRRG PRNKKRGWRR
60 70 80 90 100
LAQEPLGLEV DQFLEDVRLQ ERTSGGLLSE APNEKLFFVD TGSKEKGLTK
110 120 130 140 150
KRTKVQKKSL LLKKPLRVDL ILENTSKVPA PKDVLAHQVP NAKKLRRKEQ
160 170 180 190 200
LWEKLAKQGE LPREVRRAQA RLLNPSATRA KPGPQDTVER PFYDLWASDN
210 220 230 240 250
PLDRPLVGQD EFFLEQTKKK GVKRPARLHT KPSQAPAVEV APAGASYNPS
260 270 280 290 300
FEDHQTLLSA AHEVELQRQK EAEKLERQLA LPATEQAATQ ESTFQELCEG
310 320 330 340 350
LLEESDGEGE PGQGEGPEAG DAEVCPTPAR LATTEKKTEQ QRRREKAVHR
360 370 380 390 400
LRVQQAALRA ARLRHQELFR LRGIKAQVAL RLAELARRQR RRQARREAEA
410 420 430 440 450
DKPRRLGRLK YQAPDIDVQL SSELTDSLRT LKPEGNILRD RFKSFQRRNM
460 470
IEPRERAKFK RKYKVKLVEK RAFREIQL
Length:478
Mass (Da):54,389
Last modified:April 18, 2006 - v2
Checksum:i7F1BA13D04BFB618
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
M0QYZ9M0QYZ9_HUMAN
Ribosome biogenesis protein NOP53
NOP53
149Annotation score:
M0QZU5M0QZU5_HUMAN
Ribosome biogenesis protein NOP53
NOP53
99Annotation score:
M0QZ34M0QZ34_HUMAN
Ribosome biogenesis protein NOP53
NOP53
200Annotation score:
M0QX73M0QX73_HUMAN
Ribosome biogenesis protein NOP53
NOP53
265Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9G → R in AAG30413 (PubMed:10196275).Curated1
Sequence conflicti146 – 191RRKEQ…TVERP → SGRSSYGRSWPSRASSPGGA QGPSPVAQPFCNKGPNPAPG HRIAA in AAG30413 (PubMed:10196275).CuratedAdd BLAST46
Sequence conflicti198 – 215SDNPL…EFFLE → LNNPDKPVVWPGCLFPG in AAG30413 (PubMed:10196275).CuratedAdd BLAST18
Sequence conflicti235A → S in AAH04229 (PubMed:15489334).Curated1
Sequence conflicti417D → H in AAG30413 (PubMed:10196275).Curated1
Sequence conflicti433 – 477PEGNI…FREIQ → VLTVSCRGAPCPVMTPSLLP VPPRGYGRHHGCPWAGPVGP MPRG in CAB59242 (PubMed:17974005).CuratedAdd BLAST45
Sequence conflicti434 – 478EGNIL…REIQL → RGQHSFETGSRAFRGGI in AAG30413 (PubMed:10196275).CuratedAdd BLAST45

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02445616S → R. Corresponds to variant dbSNP:rs1042401Ensembl.1
Natural variantiVAR_011486389Q → R4 PublicationsCorresponds to variant dbSNP:rs1804994Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF182076 mRNA Translation: AAF62873.1
BC004229 mRNA Translation: AAH04229.2
BC006311 mRNA Translation: AAH06311.1
BC010095 mRNA Translation: AAH10095.1
AF296124 mRNA Translation: AAG30413.1
AL359335 mRNA Translation: CAB94786.1
AL359336 mRNA Translation: CAB94787.1
AL122063 mRNA Translation: CAB59242.1
CCDSiCCDS12705.1
RefSeqiNP_056525.2, NM_015710.4
UniGeneiHs.421907

Genome annotation databases

EnsembliENST00000246802; ENSP00000246802; ENSG00000105373
GeneIDi29997
KEGGihsa:29997
UCSCiuc002phm.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF182076 mRNA Translation: AAF62873.1
BC004229 mRNA Translation: AAH04229.2
BC006311 mRNA Translation: AAH06311.1
BC010095 mRNA Translation: AAH10095.1
AF296124 mRNA Translation: AAG30413.1
AL359335 mRNA Translation: CAB94786.1
AL359336 mRNA Translation: CAB94787.1
AL122063 mRNA Translation: CAB59242.1
CCDSiCCDS12705.1
RefSeqiNP_056525.2, NM_015710.4
UniGeneiHs.421907

3D structure databases

ProteinModelPortaliQ9NZM5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119021, 149 interactors
IntActiQ9NZM5, 34 interactors
STRINGi9606.ENSP00000246802

PTM databases

iPTMnetiQ9NZM5
PhosphoSitePlusiQ9NZM5

Polymorphism and mutation databases

BioMutaiGLTSCR2
DMDMi93141272

2D gel databases

SWISS-2DPAGEiQ9NZM5

Proteomic databases

EPDiQ9NZM5
MaxQBiQ9NZM5
PaxDbiQ9NZM5
PeptideAtlasiQ9NZM5
PRIDEiQ9NZM5
ProteomicsDBi83451

Protocols and materials databases

DNASUi29997
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000246802; ENSP00000246802; ENSG00000105373
GeneIDi29997
KEGGihsa:29997
UCSCiuc002phm.3 human

Organism-specific databases

CTDi29997
DisGeNETi29997
EuPathDBiHostDB:ENSG00000105373.18
GeneCardsiNOP53
HGNCiHGNC:4333 NOP53
HPAiHPA018999
HPA049600
MIMi605691 gene
neXtProtiNX_Q9NZM5
OpenTargetsiENSG00000105373
PharmGKBiPA28736
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2823 Eukaryota
ENOG4111GSN LUCA
GeneTreeiENSGT00390000017267
HOGENOMiHOG000070127
HOVERGENiHBG051849
InParanoidiQ9NZM5
KOiK14840
OMAiKSAWRKT
OrthoDBiEOG091G0BKI
PhylomeDBiQ9NZM5
TreeFamiTF313004

Miscellaneous databases

ChiTaRSiGLTSCR2 human
GeneWikiiGLTSCR2
GenomeRNAii29997
PROiPR:Q9NZM5
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000105373 Expressed in 230 organ(s), highest expression level in mammary gland
CleanExiHS_GLTSCR2
ExpressionAtlasiQ9NZM5 baseline and differential
GenevisibleiQ9NZM5 HS

Family and domain databases

InterProiView protein in InterPro
IPR011687 Nop53/GLTSCR2
PANTHERiPTHR14211 PTHR14211, 1 hit
PfamiView protein in Pfam
PF07767 Nop53, 1 hit
PIRSFiPIRSF017302 Gltscr2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNOP53_HUMAN
AccessioniPrimary (citable) accession number: Q9NZM5
Secondary accession number(s): Q9BTC6
, Q9HAX6, Q9NPP1, Q9NPR4, Q9UFI2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: April 18, 2006
Last modified: November 7, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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