Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Zinc finger protein 224

Gene

ZNF224

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in transcriptional regulation as a transcriptional repressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri176 – 198C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri204 – 226C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri232 – 254C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri260 – 282C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri288 – 310C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri316 – 338C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri344 – 366C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri372 – 394C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri400 – 422C2H2-type 9PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri428 – 450C2H2-type 10PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri456 – 478C2H2-type 11PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri484 – 506C2H2-type 12PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri512 – 534C2H2-type 13PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri540 – 562C2H2-type 14; degeneratePROSITE-ProRule annotationAdd BLAST23
Zinc fingeri568 – 590C2H2-type 15PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri596 – 618C2H2-type 16PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri652 – 674C2H2-type 17PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri680 – 702C2H2-type 18PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-212436 Generic Transcription Pathway
SIGNORiQ9NZL3

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 224
Alternative name(s):
Bone marrow zinc finger 2
Short name:
BMZF-2
Zinc finger protein 233
Zinc finger protein 255
Zinc finger protein 27
Zinc finger protein KOX22
Gene namesi
Name:ZNF224
Synonyms:BMZF2, KOX22, ZNF233, ZNF255, ZNF27
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000267680.5
HGNCiHGNC:13017 ZNF224
MIMi194555 gene
neXtProtiNX_Q9NZL3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi7767
OpenTargetsiENSG00000267680
PharmGKBiPA37596

Polymorphism and mutation databases

BioMutaiZNF224
DMDMi313104253

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000474651 – 707Zinc finger protein 224Add BLAST707

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki473Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki625Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ9NZL3
PaxDbiQ9NZL3
PeptideAtlasiQ9NZL3
PRIDEiQ9NZL3
ProteomicsDBi83427

PTM databases

iPTMnetiQ9NZL3
PhosphoSitePlusiQ9NZL3

Expressioni

Tissue specificityi

Ubiquitous. Mainly expressed in fetal tissues.1 Publication

Gene expression databases

BgeeiENSG00000267680 Expressed in 208 organ(s), highest expression level in oviduct epithelium
CleanExiHS_ZNF224
HS_ZNF233
ExpressionAtlasiQ9NZL3 baseline and differential
GenevisibleiQ9NZL3 HS

Organism-specific databases

HPAiHPA054045

Interactioni

Subunit structurei

Interacts with WT1. Interacts with DEPDC1A.2 Publications

Protein-protein interaction databases

BioGridi113550, 13 interactors
IntActiQ9NZL3, 1 interactor
STRINGi9606.ENSP00000337368

Structurei

Secondary structure

1707
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9NZL3
SMRiQ9NZL3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NZL3

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 78KRABPROSITE-ProRule annotationAdd BLAST71

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri176 – 198C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri204 – 226C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri232 – 254C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri260 – 282C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri288 – 310C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri316 – 338C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri344 – 366C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri372 – 394C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri400 – 422C2H2-type 9PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri428 – 450C2H2-type 10PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri456 – 478C2H2-type 11PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri484 – 506C2H2-type 12PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri512 – 534C2H2-type 13PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri540 – 562C2H2-type 14; degeneratePROSITE-ProRule annotationAdd BLAST23
Zinc fingeri568 – 590C2H2-type 15PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri596 – 618C2H2-type 16PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri652 – 674C2H2-type 17PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri680 – 702C2H2-type 18PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00930000150984
HOVERGENiHBG018163
InParanoidiQ9NZL3
KOiK09228
OMAiSHCVVHT
OrthoDBiEOG091G02KC
PhylomeDBiQ9NZL3
TreeFamiTF341885

Family and domain databases

CDDicd07765 KRAB_A-box, 1 hit
InterProiView protein in InterPro
IPR001909 KRAB
IPR036051 KRAB_dom_sf
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF01352 KRAB, 1 hit
PF00096 zf-C2H2, 14 hits
SMARTiView protein in SMART
SM00349 KRAB, 1 hit
SM00355 ZnF_C2H2, 18 hits
SUPFAMiSSF109640 SSF109640, 1 hit
SSF57667 SSF57667, 10 hits
PROSITEiView protein in PROSITE
PS50805 KRAB, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 18 hits
PS50157 ZINC_FINGER_C2H2_2, 18 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9NZL3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTTFKEAMTF KDVAVVFTEE ELGLLDLAQR KLYRDVMLEN FRNLLSVGHQ
60 70 80 90 100
AFHRDTFHFL REEKIWMMKT AIQREGNSGD KIQTEMETVS EAGTHQEWSF
110 120 130 140 150
QQIWEKIASD LTRSQDLMIN SSQFSKEGDF PCQTEAGLSV IHTRQKSSQG
160 170 180 190 200
NGYKPSFSDV SHFDFHQQLH SGEKSHTCDE CGKNFCYISA LRIHQRVHMG
210 220 230 240 250
EKCYKCDVCG KEFSQSSHLQ THQRVHTGEK PFKCVECGKG FSRRSALNVH
260 270 280 290 300
HKLHTGEKPY NCEECGKAFI HDSQLQEHQR IHTGEKPFKC DICGKSFCGR
310 320 330 340 350
SRLNRHSMVH TAEKPFRCDT CDKSFRQRSA LNSHRMIHTG EKPYKCEECG
360 370 380 390 400
KGFICRRDLY THHMVHTGEK PYNCKECGKS FRWASCLLKH QRVHSGEKPF
410 420 430 440 450
KCEECGKGFY TNSQCYSHQR SHSGEKPYKC VECGKGYKRR LDLDFHQRVH
460 470 480 490 500
TGEKLYNCKE CGKSFSRAPC LLKHERLHSG EKPFQCEECG KRFTQNSHLH
510 520 530 540 550
SHQRVHTGEK PYKCEKCGKG YNSKFNLDMH QKVHTGERPY NCKECGKSFG
560 570 580 590 600
WASCLLKHQR LHSGEKPFKC EECGKRFTQN SQLHSHQRVH TGEKPYKCDE
610 620 630 640 650
CGKGFSWSST RLTHQRRHSR ETPLKCEQHG KNIVQNSFSK VQEKVHSVEK
660 670 680 690 700
PYKCEDCGKG YNRRLNLDMH QRVHMGEKTW KCRECDMCFS QASSLRLHQN

VHVGEKP
Length:707
Mass (Da):82,280
Last modified:November 30, 2010 - v3
Checksum:iE9C1346D52BBF38F
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K7ENI7K7ENI7_HUMAN
Zinc finger protein 224
ZNF224
124Annotation score:
K7EJ30K7EJ30_HUMAN
Zinc finger protein 224
ZNF224
66Annotation score:
K7EL24K7EL24_HUMAN
Zinc finger protein 224
ZNF224
53Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti137 – 151GLSVI…SSQGN → RTICNSHKTEIFPRAI in AAD32448 (PubMed:15489334).CuratedAdd BLAST15
Sequence conflicti286K → T in CAA36579 (PubMed:12239212).Curated1
Sequence conflicti400 – 401FK → IQ in AAD32448 (PubMed:15489334).Curated2
Sequence conflicti412N → I in AAD32448 (PubMed:15489334).Curated1
Sequence conflicti521Y → H in AAD32448 (PubMed:15489334).Curated1
Sequence conflicti562H → R in AAF04106 (PubMed:12743021).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_047061118M → V4 PublicationsCorresponds to variant dbSNP:rs2068061Ensembl.1
Natural variantiVAR_024204162H → L3 PublicationsCorresponds to variant dbSNP:rs4239529Ensembl.1
Natural variantiVAR_047062438K → N. Corresponds to variant dbSNP:rs3208201Ensembl.1
Natural variantiVAR_061941447Q → H. Corresponds to variant dbSNP:rs58935748Ensembl.1
Natural variantiVAR_047063506H → D. Corresponds to variant dbSNP:rs3746323Ensembl.1
Natural variantiVAR_021891640K → E2 PublicationsCorresponds to variant dbSNP:rs3746319Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF187990 mRNA Translation: AAF04106.2
AC084219 Genomic DNA No translation available.
AC114266 Genomic DNA No translation available.
BC051902 mRNA Translation: AAH51902.1
AF067164 mRNA Translation: AAD32448.1
AY148489 mRNA Translation: AAN61121.1
X52353 mRNA Translation: CAA36579.1
AC021092 Genomic DNA Translation: AAF24968.1
CCDSiCCDS33046.1
PIRiI37962
RefSeqiNP_001308574.1, NM_001321645.1
NP_037530.2, NM_013398.3
XP_016882750.1, XM_017027261.1
UniGeneiHs.585869
Hs.709751
Hs.744152

Genome annotation databases

EnsembliENST00000336976; ENSP00000337368; ENSG00000267680
GeneIDi7767
KEGGihsa:7767
UCSCiuc002oyh.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF187990 mRNA Translation: AAF04106.2
AC084219 Genomic DNA No translation available.
AC114266 Genomic DNA No translation available.
BC051902 mRNA Translation: AAH51902.1
AF067164 mRNA Translation: AAD32448.1
AY148489 mRNA Translation: AAN61121.1
X52353 mRNA Translation: CAA36579.1
AC021092 Genomic DNA Translation: AAF24968.1
CCDSiCCDS33046.1
PIRiI37962
RefSeqiNP_001308574.1, NM_001321645.1
NP_037530.2, NM_013398.3
XP_016882750.1, XM_017027261.1
UniGeneiHs.585869
Hs.709751
Hs.744152

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ELYNMR-A227-259[»]
2ELZNMR-A647-679[»]
2EM0NMR-A675-707[»]
2EM6NMR-A199-231[»]
2EM7NMR-A339-371[»]
2EM8NMR-A423-455[»]
2EM9NMR-A367-399[»]
2EN1NMR-A563-595[»]
2EN8NMR-A171-203[»]
2ENANMR-A311-343[»]
2ENCNMR-A395-427[»]
2EOQNMR-A283-315[»]
2EORNMR-A255-287[»]
2EQ4NMR-A451-483[»]
2YSPNMR-A507-539[»]
2YTHNMR-A479-511[»]
ProteinModelPortaliQ9NZL3
SMRiQ9NZL3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113550, 13 interactors
IntActiQ9NZL3, 1 interactor
STRINGi9606.ENSP00000337368

PTM databases

iPTMnetiQ9NZL3
PhosphoSitePlusiQ9NZL3

Polymorphism and mutation databases

BioMutaiZNF224
DMDMi313104253

Proteomic databases

MaxQBiQ9NZL3
PaxDbiQ9NZL3
PeptideAtlasiQ9NZL3
PRIDEiQ9NZL3
ProteomicsDBi83427

Protocols and materials databases

DNASUi7767
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336976; ENSP00000337368; ENSG00000267680
GeneIDi7767
KEGGihsa:7767
UCSCiuc002oyh.3 human

Organism-specific databases

CTDi7767
DisGeNETi7767
EuPathDBiHostDB:ENSG00000267680.5
GeneCardsiZNF224
HGNCiHGNC:13017 ZNF224
HPAiHPA054045
MIMi194555 gene
neXtProtiNX_Q9NZL3
OpenTargetsiENSG00000267680
PharmGKBiPA37596
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00930000150984
HOVERGENiHBG018163
InParanoidiQ9NZL3
KOiK09228
OMAiSHCVVHT
OrthoDBiEOG091G02KC
PhylomeDBiQ9NZL3
TreeFamiTF341885

Enzyme and pathway databases

ReactomeiR-HSA-212436 Generic Transcription Pathway
SIGNORiQ9NZL3

Miscellaneous databases

EvolutionaryTraceiQ9NZL3
GeneWikiiZNF224
GenomeRNAii7767
PROiPR:Q9NZL3
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000267680 Expressed in 208 organ(s), highest expression level in oviduct epithelium
CleanExiHS_ZNF224
HS_ZNF233
ExpressionAtlasiQ9NZL3 baseline and differential
GenevisibleiQ9NZL3 HS

Family and domain databases

CDDicd07765 KRAB_A-box, 1 hit
InterProiView protein in InterPro
IPR001909 KRAB
IPR036051 KRAB_dom_sf
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF01352 KRAB, 1 hit
PF00096 zf-C2H2, 14 hits
SMARTiView protein in SMART
SM00349 KRAB, 1 hit
SM00355 ZnF_C2H2, 18 hits
SUPFAMiSSF109640 SSF109640, 1 hit
SSF57667 SSF57667, 10 hits
PROSITEiView protein in PROSITE
PS50805 KRAB, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 18 hits
PS50157 ZINC_FINGER_C2H2_2, 18 hits
ProtoNetiSearch...

Entry informationi

Entry nameiZN224_HUMAN
AccessioniPrimary (citable) accession number: Q9NZL3
Secondary accession number(s): A6NFW9
, P17033, Q86V10, Q8IZC8, Q9UID9, Q9Y2P6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: November 30, 2010
Last modified: November 7, 2018
This is version 175 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again