UniProtKB - Q9NZI8 (IF2B1_HUMAN)
Insulin-like growth factor 2 mRNA-binding protein 1
IGF2BP1
Functioni
GO - Molecular functioni
- mRNA 3'-UTR binding Source: UniProtKB
- mRNA 5'-UTR binding Source: BHF-UCL
- mRNA binding Source: UniProtKB
- RNA binding Source: UniProtKB
- translation regulator activity Source: BHF-UCL
GO - Biological processi
- CRD-mediated mRNA stabilization Source: UniProtKB
- mRNA transport Source: UniProtKB-KW
- negative regulation of translation Source: BHF-UCL
- nervous system development Source: GO_Central
- neuronal stem cell population maintenance Source: Ensembl
- pallium cell proliferation in forebrain Source: Ensembl
- regulation of cytokine production Source: BHF-UCL
- regulation of gene expression Source: GO_Central
- regulation of mRNA stability Source: Reactome
- regulation of mRNA stability involved in response to stress Source: UniProtKB
- regulation of RNA metabolic process Source: GO_Central
Keywordsi
Molecular function | RNA-binding |
Biological process | mRNA transport, Translation regulation, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q9NZI8 |
Reactomei | R-HSA-428359, Insulin-like Growth Factor-2 mRNA Binding Proteins (IGF2BPs/IMPs/VICKZs) bind RNA R-HSA-5687128, MAPK6/MAPK4 signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Insulin-like growth factor 2 mRNA-binding protein 1Short name: IGF2 mRNA-binding protein 1 Short name: IMP-1 Short name: IMP1 Alternative name(s): Coding region determinant-binding protein Short name: CRD-BP IGF-II mRNA-binding protein 1 VICKZ family member 1 Zipcode-binding protein 1 Short name: ZBP-1 |
Gene namesi | Name:IGF2BP1 Synonyms:CRDBP, VICKZ1, ZBP1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28866, IGF2BP1 |
MIMi | 608288, gene |
neXtProti | NX_Q9NZI8 |
VEuPathDBi | HostDB:ENSG00000159217.9 |
Subcellular locationi
Cytoplasm and Cytosol
Nucleus
Other locations
- perinuclear region
- lamellipodium
- dendrite By similarity
- dendritic spine By similarity
- growth cone
- filopodium By similarity
- axon By similarity
Note: In the nucleus, located in discrete foci, coinciding with the sites of ACTB transcription (By similarity). In the cytoplasm, localizes in cytoplasmic mRNP granules. Colocalizes with microtubules in growth cone filopodia and along neurites in neuronal cells (By similarity). Cytoplasmic colocalization with ACTB mRNA is partially lost at the cell periphery, suggesting release of the transcript. In neuronal processes, exhibits fast retrograde and anterograde movements, when associated with ACTB mRNA; this motility is lost when the association is inhibited (By similarity). In hippocampal neurons, predominantly located within dendrites, particularly at dendritic branching points in young cells, compared to axons (By similarity). In axons, predominantly found in axonal branches and their growth cones (By similarity). In motile cells, such as migrating fibroblasts, localizes to leading edges where it colocalizes with microtubules and microfilaments and to retracting tails (By similarity). Dendritic levels are regulated by neuronal activity and glutaminergic signals: they are increased by KCl-induced depolarization, which induces rapid efflux from the cell body into dendrites, and decreased by the NMDA receptor agonist (By similarity). In motile cells, transported towards the leading edge into the cortical region of the lamellipodia where it is connected to microfilaments (By similarity). In response to cellular stress, such as oxidative stress or heat shock, recruited to stress granules, but not to processing bodies.By similarity
Cytosol
- cytosol Source: HPA
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: GO_Central
Other locations
- CRD-mediated mRNA stability complex Source: UniProtKB
- cytoplasm Source: UniProtKB
- cytoplasmic stress granule Source: UniProtKB
- dendritic spine Source: UniProtKB-SubCell
- filopodium Source: UniProtKB-SubCell
- growth cone Source: UniProtKB-SubCell
- lamellipodium Source: UniProtKB-SubCell
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
- ribonucleoprotein complex Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Cell projection, Cytoplasm, Nucleus, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 213 – 214 | KE → EL: 50-fold decrease in RNA-binding affinity, decreased location in cytoplasmic RNP, increased nuclear location; when associated with 294-E-L-295 and 423-E-L-424. 1 Publication | 2 | |
Mutagenesisi | 294 – 295 | KE → EL: 50-fold decrease in RNA-binding affinity, decreased location in cytoplasmic RNP, increased nuclear location; when associated with 213-E-L-214 and 423-E-L-424. 1 Publication | 2 | |
Mutagenesisi | 423 – 424 | KK → EL: 50-fold decrease in RNA-binding affinity, decreased location in cytoplasmic RNP, increased nuclear location; when associated with 213-E-L-214 and 294-E-L-295. 1 Publication | 2 |
Organism-specific databases
DisGeNETi | 10642 |
OpenTargetsi | ENSG00000159217 |
PharmGKBi | PA143485501 |
Miscellaneous databases
Pharosi | Q9NZI8, Tbio |
Genetic variation databases
BioMutai | IGF2BP1 |
DMDMi | 296434536 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000282533 | 1 – 577 | Insulin-like growth factor 2 mRNA-binding protein 1Add BLAST | 577 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 12 | PhosphoserineCombined sources | 1 | |
Modified residuei | 73 | PhosphoserineCombined sources | 1 | |
Modified residuei | 181 | PhosphoserineCombined sources | 1 | |
Modified residuei | 528 | PhosphothreonineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9NZI8 |
jPOSTi | Q9NZI8 |
MassIVEi | Q9NZI8 |
MaxQBi | Q9NZI8 |
PaxDbi | Q9NZI8 |
PeptideAtlasi | Q9NZI8 |
PRIDEi | Q9NZI8 |
ProteomicsDBi | 11549 83410 [Q9NZI8-1] |
TopDownProteomicsi | Q9NZI8-1 [Q9NZI8-1] |
PTM databases
iPTMneti | Q9NZI8 |
PhosphoSitePlusi | Q9NZI8 |
SwissPalmi | Q9NZI8 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000159217, Expressed in lung and 86 other tissues |
Genevisiblei | Q9NZI8, HS |
Organism-specific databases
HPAi | ENSG00000159217, Group enriched (placenta, testis) |
Interactioni
Subunit structurei
Can form homodimers and heterodimers with IGF2BP1 and IGF2BP3.
Component of the coding region determinant (CRD)-mediated complex, composed of DHX9, HNRNPU, IGF2BP1, SYNCRIP and YBX1. During HCV infection, identified in a HCV IRES-mediated translation complex, at least composed of EIF3C, IGF2BP1, RPS3 and HCV RNA-replicon.
Interacts (via the KH domains) with HIV-1 GAG (via the second zinc finger motif of NC). Associates (via the RRM domains and KH domains) with HIV-1 particles.
Identified in a mRNP complex, composed of at least DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1.
Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs.
Interacts with DHX9, ELAVL2, HNRNPA2B1, HNRNPC, HNRNPH1, HNRNPU, IGF2BP2, ILF2, and YBX1.
Interacts with FMR1.
Component of a multisubunit autoregulatory RNP complex (ARC), at least composed of IGF2BP1, PABPC1 and CSDE1/UNR. Directly interacts with PABPC1.
Component of a TAU mRNP complex, at least composed of IGF2BP1, ELAVL4 and G3BP.
Interacts with ELAVL4 in an RNA-dependent manner. Associates with microtubules and polysomes.
Interacts with AGO1 and AGO2.
10 PublicationsBinary interactionsi
Hide detailsQ9NZI8
With | #Exp. | IntAct |
---|---|---|
STAU1 [O95793] | 6 | EBI-1053892,EBI-358174 |
Cbx7 [Q8VDS3] from Mus musculus. | 2 | EBI-1053892,EBI-1216533 |
Protein-protein interaction databases
BioGRIDi | 115886, 354 interactors |
ComplexPortali | CPX-1080, CRD-mediated mRNA stability complex |
CORUMi | Q9NZI8 |
DIPi | DIP-38139N |
IntActi | Q9NZI8, 89 interactors |
MINTi | Q9NZI8 |
STRINGi | 9606.ENSP00000290341 |
Miscellaneous databases
RNActi | Q9NZI8, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9NZI8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9NZI8 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 75 | RRM 1PROSITE-ProRule annotationAdd BLAST | 74 | |
Domaini | 81 – 156 | RRM 2PROSITE-ProRule annotationAdd BLAST | 76 | |
Domaini | 195 – 260 | KH 1PROSITE-ProRule annotationAdd BLAST | 66 | |
Domaini | 276 – 343 | KH 2PROSITE-ProRule annotationAdd BLAST | 68 | |
Domaini | 405 – 470 | KH 3PROSITE-ProRule annotationAdd BLAST | 66 | |
Domaini | 487 – 553 | KH 4PROSITE-ProRule annotationAdd BLAST | 67 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 187 – 570 | Necessary for interaction with ELAVL4 and binding to TAU mRNABy similarityAdd BLAST | 384 | |
Regioni | 310 – 324 | Sufficient for nuclear exportAdd BLAST | 15 | |
Regioni | 485 – 495 | Sufficient for nuclear exportAdd BLAST | 11 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG2193, Eukaryota |
GeneTreei | ENSGT00940000160427 |
HOGENOMi | CLU_020744_1_0_1 |
InParanoidi | Q9NZI8 |
OMAi | ISQGPEN |
OrthoDBi | 286875at2759 |
PhylomeDBi | Q9NZI8 |
TreeFami | TF320229 |
Family and domain databases
CDDi | cd12625, RRM1_IGF2BP1, 1 hit cd12628, RRM2_IGF2BP1, 1 hit |
Gene3Di | 3.30.1370.10, 2 hits 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR034837, IGF2BP1_RRM1 IPR034842, IGF2BP1_RRM2 IPR004087, KH_dom IPR004088, KH_dom_type_1 IPR036612, KH_dom_type_1_sf IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00013, KH_1, 4 hits PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00322, KH, 4 hits SM00360, RRM, 2 hits |
SUPFAMi | SSF54791, SSF54791, 4 hits SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50084, KH_TYPE_1, 4 hits PS50102, RRM, 2 hits |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNKLYIGNLN ESVTPADLEK VFAEHKISYS GQFLVKSGYA FVDCPDEHWA
60 70 80 90 100
MKAIETFSGK VELQGKRLEI EHSVPKKQRS RKIQIRNIPP QLRWEVLDSL
110 120 130 140 150
LAQYGTVENC EQVNTESETA VVNVTYSNRE QTRQAIMKLN GHQLENHALK
160 170 180 190 200
VSYIPDEQIA QGPENGRRGG FGSRGQPRQG SPVAAGAPAK QQQVDIPLRL
210 220 230 240 250
LVPTQYVGAI IGKEGATIRN ITKQTQSKID VHRKENAGAA EKAISVHSTP
260 270 280 290 300
EGCSSACKMI LEIMHKEAKD TKTADEVPLK ILAHNNFVGR LIGKEGRNLK
310 320 330 340 350
KVEQDTETKI TISSLQDLTL YNPERTITVK GAIENCCRAE QEIMKKVREA
360 370 380 390 400
YENDVAAMSL QSHLIPGLNL AAVGLFPASS SAVPPPPSSV TGAAPYSSFM
410 420 430 440 450
QAPEQEMVQV FIPAQAVGAI IGKKGQHIKQ LSRFASASIK IAPPETPDSK
460 470 480 490 500
VRMVIITGPP EAQFKAQGRI YGKLKEENFF GPKEEVKLET HIRVPASAAG
510 520 530 540 550
RVIGKGGKTV NELQNLTAAE VVVPRDQTPD ENDQVIVKII GHFYASQMAQ
560 570
RKIRDILAQV KQQHQKGQSN QAQARRK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 10 | Missing AA sequence (PubMed:9891060).Curated | 1 | |
Sequence conflicti | 281 | I → T in AAF37203 (Ref. 1) Curated | 1 | |
Sequence conflicti | 320 | Missing AA sequence (PubMed:9891060).Curated | 1 | |
Sequence conflicti | 365 | I → T in AAF37203 (Ref. 1) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_045366 | 135 – 273 | Missing in isoform 2. 1 PublicationAdd BLAST | 139 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF198254 mRNA Translation: AAF37203.1 DQ227344 mRNA Translation: ABB46294.1 AC091133 Genomic DNA No translation available. AC105030 Genomic DNA No translation available. |
CCDSi | CCDS11543.1 [Q9NZI8-1] CCDS54138.1 [Q9NZI8-2] |
RefSeqi | NP_001153895.1, NM_001160423.1 [Q9NZI8-2] NP_006537.3, NM_006546.3 [Q9NZI8-1] |
Genome annotation databases
Ensembli | ENST00000290341; ENSP00000290341; ENSG00000159217 [Q9NZI8-1] ENST00000431824; ENSP00000389135; ENSG00000159217 [Q9NZI8-2] |
GeneIDi | 10642 |
KEGGi | hsa:10642 |
UCSCi | uc002iom.4, human [Q9NZI8-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF198254 mRNA Translation: AAF37203.1 DQ227344 mRNA Translation: ABB46294.1 AC091133 Genomic DNA No translation available. AC105030 Genomic DNA No translation available. |
CCDSi | CCDS11543.1 [Q9NZI8-1] CCDS54138.1 [Q9NZI8-2] |
RefSeqi | NP_001153895.1, NM_001160423.1 [Q9NZI8-2] NP_006537.3, NM_006546.3 [Q9NZI8-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3KRM | X-ray | 2.75 | A/B/C | 404-566 | [»] | |
6QEY | X-ray | 2.20 | A | 194-369 | [»] | |
SMRi | Q9NZI8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115886, 354 interactors |
ComplexPortali | CPX-1080, CRD-mediated mRNA stability complex |
CORUMi | Q9NZI8 |
DIPi | DIP-38139N |
IntActi | Q9NZI8, 89 interactors |
MINTi | Q9NZI8 |
STRINGi | 9606.ENSP00000290341 |
PTM databases
iPTMneti | Q9NZI8 |
PhosphoSitePlusi | Q9NZI8 |
SwissPalmi | Q9NZI8 |
Genetic variation databases
BioMutai | IGF2BP1 |
DMDMi | 296434536 |
Proteomic databases
EPDi | Q9NZI8 |
jPOSTi | Q9NZI8 |
MassIVEi | Q9NZI8 |
MaxQBi | Q9NZI8 |
PaxDbi | Q9NZI8 |
PeptideAtlasi | Q9NZI8 |
PRIDEi | Q9NZI8 |
ProteomicsDBi | 11549 83410 [Q9NZI8-1] |
TopDownProteomicsi | Q9NZI8-1 [Q9NZI8-1] |
Protocols and materials databases
Antibodypediai | 17915, 315 antibodies |
DNASUi | 10642 |
Genome annotation databases
Ensembli | ENST00000290341; ENSP00000290341; ENSG00000159217 [Q9NZI8-1] ENST00000431824; ENSP00000389135; ENSG00000159217 [Q9NZI8-2] |
GeneIDi | 10642 |
KEGGi | hsa:10642 |
UCSCi | uc002iom.4, human [Q9NZI8-1] |
Organism-specific databases
CTDi | 10642 |
DisGeNETi | 10642 |
GeneCardsi | IGF2BP1 |
HGNCi | HGNC:28866, IGF2BP1 |
HPAi | ENSG00000159217, Group enriched (placenta, testis) |
MIMi | 608288, gene |
neXtProti | NX_Q9NZI8 |
OpenTargetsi | ENSG00000159217 |
PharmGKBi | PA143485501 |
VEuPathDBi | HostDB:ENSG00000159217.9 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2193, Eukaryota |
GeneTreei | ENSGT00940000160427 |
HOGENOMi | CLU_020744_1_0_1 |
InParanoidi | Q9NZI8 |
OMAi | ISQGPEN |
OrthoDBi | 286875at2759 |
PhylomeDBi | Q9NZI8 |
TreeFami | TF320229 |
Enzyme and pathway databases
PathwayCommonsi | Q9NZI8 |
Reactomei | R-HSA-428359, Insulin-like Growth Factor-2 mRNA Binding Proteins (IGF2BPs/IMPs/VICKZs) bind RNA R-HSA-5687128, MAPK6/MAPK4 signaling |
Miscellaneous databases
BioGRID-ORCSi | 10642, 26 hits in 1008 CRISPR screens |
ChiTaRSi | IGF2BP1, human |
EvolutionaryTracei | Q9NZI8 |
GeneWikii | IGF2BP1 |
GenomeRNAii | 10642 |
Pharosi | Q9NZI8, Tbio |
PROi | PR:Q9NZI8 |
RNActi | Q9NZI8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000159217, Expressed in lung and 86 other tissues |
Genevisiblei | Q9NZI8, HS |
Family and domain databases
CDDi | cd12625, RRM1_IGF2BP1, 1 hit cd12628, RRM2_IGF2BP1, 1 hit |
Gene3Di | 3.30.1370.10, 2 hits 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR034837, IGF2BP1_RRM1 IPR034842, IGF2BP1_RRM2 IPR004087, KH_dom IPR004088, KH_dom_type_1 IPR036612, KH_dom_type_1_sf IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00013, KH_1, 4 hits PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00322, KH, 4 hits SM00360, RRM, 2 hits |
SUPFAMi | SSF54791, SSF54791, 4 hits SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50084, KH_TYPE_1, 4 hits PS50102, RRM, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IF2B1_HUMAN | |
Accessioni | Q9NZI8Primary (citable) accession number: Q9NZI8 Secondary accession number(s): C9JT33 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2007 |
Last sequence update: | May 18, 2010 | |
Last modified: | April 7, 2021 | |
This is version 181 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families