UniProtKB - Q9NZ09 (UBAP1_HUMAN)
Ubiquitin-associated protein 1
UBAP1
Functioni
Component of the ESCRT-I complex, a regulator of vesicular trafficking process (PubMed:21757351, PubMed:22405001, PubMed:31203368).
Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs) (PubMed:21757351, PubMed:22405001).
Plays a role in the proteasomal degradation of ubiquitinated cell-surface proteins, such as EGFR and BST2 (PubMed:24284069, PubMed:22405001, PubMed:31203368).
4 PublicationsCaution
GO - Molecular functioni
- ubiquitin binding Source: UniProtKB
GO - Biological processi
- protein transport Source: UniProtKB-KW
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: UniProtKB
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q9NZ09 |
Reactomei | R-HSA-162588, Budding and maturation of HIV virion R-HSA-174490, Membrane binding and targetting of GAG proteins R-HSA-917729, Endosomal Sorting Complex Required For Transport (ESCRT) R-HSA-9610379, HCMV Late Events R-HSA-9615710, Late endosomal microautophagy |
SignaLinki | Q9NZ09 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-associated protein 11 PublicationShort name: UBAP-11 Publication Alternative name(s): Nasopharyngeal carcinoma-associated gene 20 protein |
Gene namesi | ORF Names:NAG20 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:12461, UBAP1 |
MIMi | 609787, gene |
neXtProti | NX_Q9NZ09 |
VEuPathDBi | HostDB:ENSG00000165006 |
Subcellular locationi
Cytosol
- cytosol Source: UniProtKB
Endosome
- endosome membrane Source: Reactome
- ESCRT I complex Source: UniProtKB
Plasma Membrane
- plasma membrane Source: HPA
Other locations
- cytoplasm Source: LIFEdb
- intracellular membrane-bounded organelle Source: HPA
Keywords - Cellular componenti
Cytoplasm, EndosomePathology & Biotechi
Involvement in diseasei
Spastic paraplegia 80, autosomal dominant (SPG80)2 Publications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_082199 | 125 – 502 | Missing in SPG80. 1 PublicationAdd BLAST | 378 | |
Natural variantiVAR_082200 | 176 – 502 | Missing in SPG80; unknown pathological significance. 1 PublicationAdd BLAST | 327 |
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 17 – 19 | LDD → AAA: Abolishes association with the ESCRT-I complex. 1 Publication | 3 | |
Mutagenesisi | 20 – 22 | VPF → AAA: Abolishes association with the ESCRT-I complex. 1 Publication | 3 | |
Mutagenesisi | 37 | P → A: Abolishes association with the ESCRT-I complex. 1 Publication | 1 | |
Mutagenesisi | 59 | E → G: Abolishes association with the ESCRT-I complex. 1 Publication | 1 | |
Mutagenesisi | 268 | F → S: Abolished interaction with PTPN23. 1 Publication | 1 | |
Mutagenesisi | 269 | P → A: Does not affect interaction with PTPN23. 1 Publication | 1 | |
Mutagenesisi | 271 | L → A: Does not affect interaction with PTPN23. 1 Publication | 1 | |
Mutagenesisi | 404 | Y → A: Strongly reduced interaction with ubiquitinated proteins. 1 Publication | 1 | |
Mutagenesisi | 472 | F → A: Strongly reduced interaction with ubiquitinated proteins. 1 Publication | 1 |
Keywords - Diseasei
Disease variant, Hereditary spastic paraplegia, NeurodegenerationOrganism-specific databases
DisGeNETi | 51271 |
MalaCardsi | UBAP1 |
MIMi | 618418, phenotype |
OpenTargetsi | ENSG00000165006 |
Orphaneti | 100993, Autosomal dominant spastic paraplegia type 12 |
PharmGKBi | PA37111 |
Miscellaneous databases
Pharosi | Q9NZ09, Tbio |
Genetic variation databases
BioMutai | UBAP1 |
DMDMi | 67475018 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000211017 | 1 – 502 | Ubiquitin-associated protein 1Add BLAST | 502 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 146 | PhosphoserineCombined sources | 1 | |
Modified residuei | 205 | PhosphoserineCombined sources | 1 | |
Modified residuei | 289 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9NZ09 |
jPOSTi | Q9NZ09 |
MassIVEi | Q9NZ09 |
MaxQBi | Q9NZ09 |
PaxDbi | Q9NZ09 |
PeptideAtlasi | Q9NZ09 |
PRIDEi | Q9NZ09 |
ProteomicsDBi | 24396 25363 83314 [Q9NZ09-1] 83315 [Q9NZ09-2] |
PTM databases
GlyGeni | Q9NZ09, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9NZ09 |
PhosphoSitePlusi | Q9NZ09 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000165006, Expressed in vagina and 238 other tissues |
ExpressionAtlasi | Q9NZ09, baseline and differential |
Genevisiblei | Q9NZ09, HS |
Organism-specific databases
HPAi | ENSG00000165006, Low tissue specificity |
Interactioni
Subunit structurei
Component of an ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1 stoichiometry (PubMed:21757351, PubMed:24284069, PubMed:22405001, PubMed:31203368) (Probable).
Interacts with PTPN23 (PubMed:21757351, PubMed:27839950).
Interacts (via UBA domains) with ubiquitinated proteins (PubMed:22405001, PubMed:30929741).
1 Publication6 PublicationsBinary interactionsi
Q9NZ09
With | #Exp. | IntAct |
---|---|---|
PPIC [P45877] | 3 | EBI-9641159,EBI-953909 |
GO - Molecular functioni
- ubiquitin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 119424, 30 interactors |
DIPi | DIP-47291N |
IntActi | Q9NZ09, 11 interactors |
MINTi | Q9NZ09 |
STRINGi | 9606.ENSP00000297661 |
Miscellaneous databases
RNActi | Q9NZ09, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9NZ09 |
BMRBi | Q9NZ09 |
SMRi | Q9NZ09 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9NZ09 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 17 – 63 | UMAPROSITE-ProRule annotationAdd BLAST | 47 | |
Domaini | 389 – 430 | UBA 1PROSITE-ProRule annotationAdd BLAST | 42 | |
Domaini | 451 – 498 | UBA 2PROSITE-ProRule annotationAdd BLAST | 48 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 95 | Interaction with ESCRT-I1 PublicationAdd BLAST | 95 | |
Regioni | 86 – 117 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 260 – 290 | Interaction with PTPN231 PublicationAdd BLAST | 31 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 96 – 117 | Polar residuesSequence analysisAdd BLAST | 22 |
Domaini
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | ENOG502QTJC, Eukaryota |
GeneTreei | ENSGT00390000008092 |
HOGENOMi | CLU_041679_0_0_1 |
InParanoidi | Q9NZ09 |
OMAi | ETQYDFS |
OrthoDBi | 683409at2759 |
PhylomeDBi | Q9NZ09 |
TreeFami | TF329247 |
Family and domain databases
Gene3Di | 1.20.120.1920, 1 hit |
InterProi | View protein in InterPro IPR015940, UBA IPR009060, UBA-like_sf IPR038870, UBAP1 IPR042575, UBAP1_C IPR023340, UMA |
PANTHERi | PTHR15960, PTHR15960, 1 hit |
SUPFAMi | SSF46934, SSF46934, 2 hits |
PROSITEi | View protein in PROSITE PS50030, UBA, 2 hits PS51497, UMA, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASKKLGADF HGTFSYLDDV PFKTGDKFKT PAKVGLPIGF SLPDCLQVVR
60 70 80 90 100
EVQYDFSLEK KTIEWAEEIK KIEEAEREAE CKIAEAEAKV NSKSGPEGDS
110 120 130 140 150
KMSFSKTHST ATMPPPINPI LASLQHNSIL TPTRVSSSAT KQKVLSPPHI
160 170 180 190 200
KADFNLADFE CEEDPFDNLE LKTIDEKEEL RNILVGTTGP IMAQLLDNNL
210 220 230 240 250
PRGGSGSVLQ DEEVLASLER ATLDFKPLHK PNGFITLPQL GNCEKMSLSS
260 270 280 290 300
KVSLPPIPAV SNIKSLSFPK LDSDDSNQKT AKLASTFHST SCLRNGTFQN
310 320 330 340 350
SLKPSTQSSA SELNGHHTLG LSALNLDSGT EMPALTSSQM PSLSVLSVCT
360 370 380 390 400
EESSPPNTGP TVTPPNFSVS QVPNMPSCPQ AYSELQMLSP SERQCVETVV
410 420 430 440 450
NMGYSYECVL RAMKKKGENI EQILDYLFAH GQLCEKGFDP LLVEEALEMH
460 470 480 490 500
QCSEEKMMEF LQLMSKFKEM GFELKDIKEV LLLHNNDQDN ALEDLMARAG
AS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0D9SG79 | A0A0D9SG79_HUMAN | Ubiquitin-associated protein 1 | UBAP1 | 528 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 81 | C → R in CAG38582 (Ref. 4) Curated | 1 | |
Sequence conflicti | 187 | T → N in BAH14025 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 331 | E → D in BAC11176 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 398 | T → M in BAH12084 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 403 | G → C in BAC11323 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_082199 | 125 – 502 | Missing in SPG80. 1 PublicationAdd BLAST | 378 | |
Natural variantiVAR_082200 | 176 – 502 | Missing in SPG80; unknown pathological significance. 1 PublicationAdd BLAST | 327 | |
Natural variantiVAR_034577 | 357 | N → K1 PublicationCorresponds to variant dbSNP:rs16935457Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046866 | 1 – 53 | MASKK…VREVQ → MSGAVRGRRREVGLQHGGCG TGGGYGDGLARSGQSWRWWR SLSLGAVGSSAGTEPGRPAG ASTFRLLRRRQQRHSGSKWL LRSWVQIFM in isoform 3. 1 PublicationAdd BLAST | 53 | |
Alternative sequenceiVSP_046867 | 1 – 11 | MASKKLGADFH → MSGAVRGRRREVGLQHGGCG TGGGYGDGLARSGQSWRWWR SLSLGAVGSSAGTEPGRPAG ASTFRLLRRRQQRHS in isoform 4. 1 PublicationAdd BLAST | 11 | |
Alternative sequenceiVSP_013650 | 362 – 422 | Missing in isoform 2. CuratedAdd BLAST | 61 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000297661.9; ENSP00000297661.4; ENSG00000165006.14 ENST00000359544.2; ENSP00000352541.2; ENSG00000165006.14 ENST00000379186.8; ENSP00000368484.3; ENSG00000165006.14 [Q9NZ09-2] ENST00000625521.2; ENSP00000486574.1; ENSG00000165006.14 [Q9NZ09-4] |
GeneIDi | 51271 |
KEGGi | hsa:51271 |
MANE-Selecti | ENST00000297661.9; ENSP00000297661.4; NM_016525.5; NP_057609.2 |
UCSCi | uc003ztx.4, human [Q9NZ09-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WGN | NMR | - | A | 381-430 | [»] | |
4AE4 | X-ray | 1.65 | A/B | 389-502 | [»] | |
5LM1 | X-ray | 2.55 | B | 261-280 | [»] | |
AlphaFoldDBi | Q9NZ09 | |||||
BMRBi | Q9NZ09 | |||||
SMRi | Q9NZ09 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 119424, 30 interactors |
DIPi | DIP-47291N |
IntActi | Q9NZ09, 11 interactors |
MINTi | Q9NZ09 |
STRINGi | 9606.ENSP00000297661 |
PTM databases
GlyGeni | Q9NZ09, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9NZ09 |
PhosphoSitePlusi | Q9NZ09 |
Genetic variation databases
BioMutai | UBAP1 |
DMDMi | 67475018 |
Proteomic databases
EPDi | Q9NZ09 |
jPOSTi | Q9NZ09 |
MassIVEi | Q9NZ09 |
MaxQBi | Q9NZ09 |
PaxDbi | Q9NZ09 |
PeptideAtlasi | Q9NZ09 |
PRIDEi | Q9NZ09 |
ProteomicsDBi | 24396 25363 83314 [Q9NZ09-1] 83315 [Q9NZ09-2] |
Protocols and materials databases
Antibodypediai | 11158, 210 antibodies from 30 providers |
DNASUi | 51271 |
Genome annotation databases
Ensembli | ENST00000297661.9; ENSP00000297661.4; ENSG00000165006.14 ENST00000359544.2; ENSP00000352541.2; ENSG00000165006.14 ENST00000379186.8; ENSP00000368484.3; ENSG00000165006.14 [Q9NZ09-2] ENST00000625521.2; ENSP00000486574.1; ENSG00000165006.14 [Q9NZ09-4] |
GeneIDi | 51271 |
KEGGi | hsa:51271 |
MANE-Selecti | ENST00000297661.9; ENSP00000297661.4; NM_016525.5; NP_057609.2 |
UCSCi | uc003ztx.4, human [Q9NZ09-1] |
Organism-specific databases
CTDi | 51271 |
DisGeNETi | 51271 |
GeneCardsi | UBAP1 |
HGNCi | HGNC:12461, UBAP1 |
HPAi | ENSG00000165006, Low tissue specificity |
MalaCardsi | UBAP1 |
MIMi | 609787, gene 618418, phenotype |
neXtProti | NX_Q9NZ09 |
OpenTargetsi | ENSG00000165006 |
Orphaneti | 100993, Autosomal dominant spastic paraplegia type 12 |
PharmGKBi | PA37111 |
VEuPathDBi | HostDB:ENSG00000165006 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QTJC, Eukaryota |
GeneTreei | ENSGT00390000008092 |
HOGENOMi | CLU_041679_0_0_1 |
InParanoidi | Q9NZ09 |
OMAi | ETQYDFS |
OrthoDBi | 683409at2759 |
PhylomeDBi | Q9NZ09 |
TreeFami | TF329247 |
Enzyme and pathway databases
PathwayCommonsi | Q9NZ09 |
Reactomei | R-HSA-162588, Budding and maturation of HIV virion R-HSA-174490, Membrane binding and targetting of GAG proteins R-HSA-917729, Endosomal Sorting Complex Required For Transport (ESCRT) R-HSA-9610379, HCMV Late Events R-HSA-9615710, Late endosomal microautophagy |
SignaLinki | Q9NZ09 |
Miscellaneous databases
BioGRID-ORCSi | 51271, 604 hits in 1085 CRISPR screens |
ChiTaRSi | UBAP1, human |
EvolutionaryTracei | Q9NZ09 |
GeneWikii | UBAP1 |
GenomeRNAii | 51271 |
Pharosi | Q9NZ09, Tbio |
PROi | PR:Q9NZ09 |
RNActi | Q9NZ09, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000165006, Expressed in vagina and 238 other tissues |
ExpressionAtlasi | Q9NZ09, baseline and differential |
Genevisiblei | Q9NZ09, HS |
Family and domain databases
Gene3Di | 1.20.120.1920, 1 hit |
InterProi | View protein in InterPro IPR015940, UBA IPR009060, UBA-like_sf IPR038870, UBAP1 IPR042575, UBAP1_C IPR023340, UMA |
PANTHERi | PTHR15960, PTHR15960, 1 hit |
SUPFAMi | SSF46934, SSF46934, 2 hits |
PROSITEi | View protein in PROSITE PS50030, UBA, 2 hits PS51497, UMA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | UBAP1_HUMAN | |
Accessioni | Q9NZ09Primary (citable) accession number: Q9NZ09 Secondary accession number(s): B7Z348 Q8NCH9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2005 |
Last sequence update: | October 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 177 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references