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UniProtKB - Q9NYQ2 (HAOX2_MOUSE)
Protein
2-Hydroxyacid oxidase 2
Gene
Hao2
Organism
Mus musculus (Mouse)
Status
Functioni
Oxidase that catalyzes the oxidation of medium chain hydroxyacids such as 2-hydroxyoctanoate, to the correspondong 2-oxoacids. Its role in the oxidation of 2-hydroxy fatty acids may contribute to the general pathway of fatty acid alpha-oxidation. Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2. Is not active on glycolate, glyoxylate, L-lactate, 2-hydroxybutanoate and 2-hydroxyhexadecanoate.
1 PublicationCaution
Was originally thought to originate from human.1 Publication
Catalytic activityi
- EC:1.1.3.151 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Cofactori
FMNBy similarity
: fatty acid metabolism Pathwayi
This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 106 | FMNBy similarity | 1 | |
Binding sitei | 128 | FMNBy similarity | 1 | |
Binding sitei | 130 | 2-oxocarboxylatePROSITE-ProRule annotation | 1 | |
Binding sitei | 156 | FMNBy similarity | 1 | |
Binding sitei | 165 | 2-oxocarboxylatePROSITE-ProRule annotation | 1 | |
Binding sitei | 224 | FMNBy similarity | 1 | |
Active sitei | 248 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Binding sitei | 251 | 2-oxocarboxylatePROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 77 – 79 | FMNBy similarity | 3 | |
Nucleotide bindingi | 279 – 283 | FMNBy similarity | 5 | |
Nucleotide bindingi | 302 – 303 | FMNBy similarity | 2 |
GO - Molecular functioni
- (S)-2-hydroxy-acid oxidase activity Source: MGI
- FMN binding Source: MGI
- identical protein binding Source: MGI
GO - Biological processi
- fatty acid oxidation Source: MGI
- mandelate metabolic process Source: MGI
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | Flavoprotein, FMN |
Enzyme and pathway databases
BRENDAi | 1.1.3.15, 3474 |
Reactomei | R-MMU-390918, Peroxisomal lipid metabolism R-MMU-9033241, Peroxisomal protein import |
UniPathwayi | UPA00199 |
Names & Taxonomyi
Protein namesi | Recommended name: 2-Hydroxyacid oxidase 2 (EC:1.1.3.151 Publication)Short name: HAOX2 Alternative name(s): (S)-2-hydroxy-acid oxidase, peroxisomal Medium chain alpha-hydroxy acid oxidase Medium-chain L-2-hydroxy acid oxidase |
Gene namesi | Name:Hao2 Synonyms:Hao3, Haox2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96012, Hao2 |
VEuPathDBi | HostDB:ENSMUSG00000027870 |
Subcellular locationi
Peroxisome
- Peroxisome 1 Publication
Mitochondrion
- mitochondrion Source: MGI
Peroxisome
- peroxisomal matrix Source: Ensembl
- peroxisome Source: MGI
Keywords - Cellular componenti
PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000206321 | 1 – 353 | 2-Hydroxyacid oxidase 2Add BLAST | 353 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 171 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q9NYQ2 |
MaxQBi | Q9NYQ2 |
PaxDbi | Q9NYQ2 |
PRIDEi | Q9NYQ2 |
ProteomicsDBi | 269764 |
PTM databases
iPTMneti | Q9NYQ2 |
PhosphoSitePlusi | Q9NYQ2 |
Expressioni
Tissue specificityi
Pancreas.
Gene expression databases
Bgeei | ENSMUSG00000027870, Expressed in adrenal gland and 96 other tissues |
Genevisiblei | Q9NYQ2, MM |
Interactioni
Subunit structurei
Homotetramer.
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000029464 |
Miscellaneous databases
RNActi | Q9NYQ2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 353 | FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd BLAST | 352 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 351 – 353 | Microbody targeting signalSequence analysis | 3 |
Sequence similaritiesi
Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0538, Eukaryota |
GeneTreei | ENSGT00390000018717 |
HOGENOMi | CLU_020639_6_1_1 |
InParanoidi | Q9NYQ2 |
OMAi | FQYEIYL |
OrthoDBi | 879633at2759 |
PhylomeDBi | Q9NYQ2 |
TreeFami | TF313363 |
Family and domain databases
CDDi | cd02809, alpha_hydroxyacid_oxid_FMN, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR012133, Alpha-hydoxy_acid_DH_FMN IPR000262, FMN-dep_DH IPR037396, FMN_HAD IPR008259, FMN_hydac_DH_AS |
Pfami | View protein in Pfam PF01070, FMN_dh, 1 hit |
PIRSFi | PIRSF000138, Al-hdrx_acd_dh, 1 hit |
PROSITEi | View protein in PROSITE PS00557, FMN_HYDROXY_ACID_DH_1, 1 hit PS51349, FMN_HYDROXY_ACID_DH_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9NYQ2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLLCLADFK AQAQKQLSKT SWDFIEGEAD DGITYNDNLA AFRRIRLRPR
60 70 80 90 100
YLRDVSKIDT RTTIQGQEIN APICISPTAF HSIAWADGEK STAKAAQKAN
110 120 130 140 150
ICYVISSYAS YTVEDIVAAA PGGLHWFQLY VQPDWDINKQ MVQRIEALGF
160 170 180 190 200
KALVVTVDAP VLGNRRGNKR SLLDLEANIK LKDLRSPGES KSGLPTPLSM
210 220 230 240 250
PSSSSCWNDL PLLQSMTRLP IILKGILTKE DAELAVKHNI RGIIVSNHGG
260 270 280 290 300
RQLDEVPASI DALREVVAAV NGKIEVYMDG GVRTGNDVLK ALALGARCIF
310 320 330 340 350
LGRPIIWGLA CKGEDGVKEV LDILKEELHT CMALSGCRSV AEISPDLIQF
SRL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 164 | N → H in CAB96380 (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF231918 mRNA Translation: AAF40201.1 AF272947 mRNA Translation: AAF81795.1 AJ251820 mRNA Translation: CAB96380.1 AK078908 mRNA Translation: BAC37452.1 |
CCDSi | CCDS17671.1 |
RefSeqi | NP_062418.3, NM_019545.4 XP_011238471.1, XM_011240169.2 |
Genome annotation databases
Ensembli | ENSMUST00000029464; ENSMUSP00000029464; ENSMUSG00000027870 |
GeneIDi | 56185 |
KEGGi | mmu:56185 |
UCSCi | uc008qqj.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF231918 mRNA Translation: AAF40201.1 AF272947 mRNA Translation: AAF81795.1 AJ251820 mRNA Translation: CAB96380.1 AK078908 mRNA Translation: BAC37452.1 |
CCDSi | CCDS17671.1 |
RefSeqi | NP_062418.3, NM_019545.4 XP_011238471.1, XM_011240169.2 |
3D structure databases
SMRi | Q9NYQ2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000029464 |
PTM databases
iPTMneti | Q9NYQ2 |
PhosphoSitePlusi | Q9NYQ2 |
Proteomic databases
jPOSTi | Q9NYQ2 |
MaxQBi | Q9NYQ2 |
PaxDbi | Q9NYQ2 |
PRIDEi | Q9NYQ2 |
ProteomicsDBi | 269764 |
Protocols and materials databases
Antibodypediai | 33905, 117 antibodies from 21 providers |
DNASUi | 56185 |
Genome annotation databases
Ensembli | ENSMUST00000029464; ENSMUSP00000029464; ENSMUSG00000027870 |
GeneIDi | 56185 |
KEGGi | mmu:56185 |
UCSCi | uc008qqj.2, mouse |
Organism-specific databases
CTDi | 51179 |
MGIi | MGI:96012, Hao2 |
VEuPathDBi | HostDB:ENSMUSG00000027870 |
Phylogenomic databases
eggNOGi | KOG0538, Eukaryota |
GeneTreei | ENSGT00390000018717 |
HOGENOMi | CLU_020639_6_1_1 |
InParanoidi | Q9NYQ2 |
OMAi | FQYEIYL |
OrthoDBi | 879633at2759 |
PhylomeDBi | Q9NYQ2 |
TreeFami | TF313363 |
Enzyme and pathway databases
UniPathwayi | UPA00199 |
BRENDAi | 1.1.3.15, 3474 |
Reactomei | R-MMU-390918, Peroxisomal lipid metabolism R-MMU-9033241, Peroxisomal protein import |
Miscellaneous databases
BioGRID-ORCSi | 56185, 1 hit in 67 CRISPR screens |
ChiTaRSi | Hao2, mouse |
PROi | PR:Q9NYQ2 |
RNActi | Q9NYQ2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027870, Expressed in adrenal gland and 96 other tissues |
Genevisiblei | Q9NYQ2, MM |
Family and domain databases
CDDi | cd02809, alpha_hydroxyacid_oxid_FMN, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR012133, Alpha-hydoxy_acid_DH_FMN IPR000262, FMN-dep_DH IPR037396, FMN_HAD IPR008259, FMN_hydac_DH_AS |
Pfami | View protein in Pfam PF01070, FMN_dh, 1 hit |
PIRSFi | PIRSF000138, Al-hdrx_acd_dh, 1 hit |
PROSITEi | View protein in PROSITE PS00557, FMN_HYDROXY_ACID_DH_1, 1 hit PS51349, FMN_HYDROXY_ACID_DH_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HAOX2_MOUSE | |
Accessioni | Q9NYQ2Primary (citable) accession number: Q9NYQ2 Secondary accession number(s): Q9JHS7, Q9JI00 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | October 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families