MAP3K20

Functionⁱ

Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2 (PubMed:10924358, PubMed:11836244, PubMed:15342622, PubMed:21224381). Involved in limb development (PubMed:26755636).5 Publications

Isoform 1: Phosphorylates histone H3 at 'Ser-28' (PubMed:15684425). May have role in neoplastic cell transformation and cancer development (PubMed:15172994). Causes cell shrinkage and disruption of actin stress fibers (PubMed:11042189).3 Publications

Catalytic activityⁱ

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactorⁱ

Mg²⁺1 Publication

Activity regulationⁱ

Activated by phosphorylation by PKN1 and autophosphorylation on Thr-161 and Ser-165.3 Publications

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	45	ATPPROSITE-ProRule annotation1 Publication		1
Active siteⁱ	133	Proton acceptorPROSITE-ProRule annotationBy similarity		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	22 – 30	ATPPROSITE-ProRule annotationBy similarity		9

GO - Molecular functionⁱ

ATP binding
Source: UniProtKB
Inferred from direct assayⁱ
- 11836244
magnesium ion binding
Source: UniProtKB
Inferred from direct assayⁱ
- 11836244
MAP kinase kinase kinase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 11836244
protein serine/threonine kinase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 11836244
RNA binding
Source: UniProtKB
Inferred from high throughput direct assayⁱ
- 22681889

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

activation of JUN kinase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 11836244
activation of MAPKK activity
Source: UniProtKB
Traceable author statementⁱ
- 11836244
cell cycle arrest
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 11836244
cell death
Source: UniProtKB
Non-traceable author statementⁱ
- 11836244
cell differentiation
Source: UniProtKB
Non-traceable author statementⁱ
- 11836244
cell proliferation
Source: UniProtKB
Non-traceable author statementⁱ
- 11836244
cellular response to gamma radiation
Source: UniProtKB
Inferred from direct assayⁱ
- 11836244
cytoskeleton organization Source: Ensembl
DNA damage checkpoint
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 11836244
embryonic digit morphogenesis
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 26755636
limb development
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 26755636
mitotic cell cycle checkpoint
Source: UniProtKB
Inferred from direct assayⁱ
- 15342622
positive regulation of apoptotic process
Source: UniProtKB
Inferred from direct assayⁱ
- 10924358
protein phosphorylation
Source: UniProtKB
Inferred from direct assayⁱ
- 11836244
stress-activated MAPK cascade
Source: UniProtKB
Inferred from direct assayⁱ
- 11836244

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Serine/threonine-protein kinase, Transferase
Biological process	Cell cycle
Ligand	ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkⁱ	Q9NYL2
SIGNORⁱ	Q9NYL2

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Mitogen-activated protein kinase kinase kinase 20Imported (EC:2.7.11.25) Alternative name(s): Human cervical cancer suppressor gene 4 protein Short name: HCCS-4 Leucine zipper- and sterile alpha motif-containing kinase MLK-like mitogen-activated protein triple kinase Mitogen-activated protein kinase kinase kinase MLT Mixed lineage kinase-related kinase Short name: MLK-related kinase Short name: MRK Sterile alpha motif- and leucine zipper-containing kinase AZK
Gene namesⁱ	Name:MAP3K20Imported Synonyms:MLTK, ZAK1 Publication ORF Names:HCCS4
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 2

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000091436.16
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:17797 MAP3K20
MIMⁱ	609479 gene
neXtProtⁱ	NX_Q9NYL2

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Involvement in diseaseⁱ

Split-foot malformation with mesoaxial polydactyly (SFMMP)1 Publication

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionAn autosomal recessive disorder characterized by a split-foot defect, mesoaxial polydactyly, nail abnormalities of the hands, and sensorineural hearing loss.

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_076448	368	F → C in SFMMP; produces protein aggregation. 1 PublicationCorresponds to variant dbSNP:rs863225437EnsemblClinVar.		1

Myopathy, centronuclear, 6, with fiber-type disproportion (CNM6)1 Publication

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionA form of centronuclear myopathy, a congenital muscle disorder characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers. CNM6 is an autosomal recessive, slowly progressive form with onset in infancy or early childhood.

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	45	K → M: Loss of kinase activity. 1 Publication	1
Mutagenesisⁱ	161	T → A: Loss of autophosphorylation activity. 1 Publication	1
Mutagenesisⁱ	162	T → A: Slight loss of autophosphorylation activity. 1 Publication	1
Mutagenesisⁱ	165	S → A: Loss of autophosphorylation activity. 1 Publication	1

Keywords - Diseaseⁱ

Disease mutation

Organism-specific databases

DisGeNET More...DisGeNETⁱ	51776
MalaCards human disease database More...MalaCardsⁱ	MAP3K20
MIMⁱ	616890 phenotype 617760 phenotype
Open Targets More...OpenTargetsⁱ	ENSG00000091436

Chemistry databases

ChEMBLⁱ	CHEMBL3886
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	2289

Polymorphism and mutation databases

BioMutaⁱ	MLTK
DMDMⁱ	313104215

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedCombined sources
ChainⁱPRO_0000086338	2 – 800	Mitogen-activated protein kinase kinase kinase 20Add BLAST		799

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylserineCombined sources	1
Modified residueⁱ	161	Phosphothreonine; by autocatalysis1 Publication	1
Modified residueⁱ	165	Phosphoserine; by autocatalysis1 Publication	1
Modified residueⁱ	275	PhosphoserineCombined sources	1
Modified residueⁱ	302	PhosphoserineCombined sources	1
Modified residueⁱ	567	PhosphoserineCombined sources	1
Modified residueⁱ	593	PhosphoserineCombined sources	1
Modified residueⁱ	599	PhosphoserineCombined sources	1
Modified residueⁱ	628	PhosphothreonineCombined sources	1
Modified residueⁱ	633	PhosphoserineCombined sources	1
Modified residueⁱ	637	PhosphoserineCombined sources	1
Modified residueⁱ	648	PhosphoserineCombined sources	1
Modified residueⁱ	649	PhosphoserineCombined sources	1
Modified residueⁱ	685	PhosphoserineCombined sources	1
Modified residueⁱ	727	PhosphoserineCombined sources	1
Modified residueⁱ	733	PhosphoserineCombined sources	1
Isoform 21 Publication (identifier: Q9NYL2-2)
Modified residueⁱ	339	PhosphoserineCombined sources1 Publication	1
Modified residueⁱ	429	PhosphoserineCombined sources1 Publication	1
Modified residueⁱ	434	PhosphoserineCombined sources1 Publication	1
Modified residueⁱ	454	PhosphoserineCombined sources1 Publication	1

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	Q9NYL2
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9NYL2
PaxDbⁱ	Q9NYL2
PeptideAtlas More...PeptideAtlasⁱ	Q9NYL2
PRIDEⁱ	Q9NYL2
ProteomicsDBⁱ	83246 83247 [Q9NYL2-2] 83248 [Q9NYL2-3]

PTM databases

iPTMnetⁱ	Q9NYL2
PhosphoSitePlusⁱ	Q9NYL2

Expressionⁱ

Tissue specificityⁱ

Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed.2 Publications

Gene expression databases

Bgeeⁱ	ENSG00000091436 Expressed in 221 organ(s), highest expression level in heart left ventricle
ExpressionAtlasⁱ	Q9NYL2 baseline and differential
Genevisibleⁱ	Q9NYL2 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA017205

HPAⁱ

HPA017205

Interactionⁱ

Subunit structureⁱ

Homodimer (PubMed:10924358, PubMed:26755636). Interacts with PKN1 and ZNF33A (PubMed:12535642, PubMed:12761180). Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, MAP3K20 and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and MAP3K20 (PubMed:21224381).5 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
PKN1	Q16512	2	EBI-687346,EBI-602382
RPS6KA5	O75582	4	EBI-602273,EBI-73869
YWHAZ	P63104	4	EBI-602273,EBI-347088
ZFP2	Q6ZN57	5	EBI-10255081,EBI-7236323
ZNF567	Q8N184	5	EBI-10255081,EBI-749400

Protein-protein interaction databases

BioGridⁱ	119725, 44 interactors
IntActⁱ	Q9NYL2, 40 interactors
Molecular INTeraction database More...MINTⁱ	Q9NYL2
STRINGⁱ	9606.ENSP00000364361

Chemistry databases

BindingDBⁱ

Q9NYL2

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	13 – 15	Combined sources	3
Beta strandⁱ	16 – 20	Combined sources	5
Helixⁱ	22 – 25	Combined sources	4
Beta strandⁱ	30 – 35	Combined sources	6
Turnⁱ	36 – 39	Combined sources	4
Beta strandⁱ	40 – 48	Combined sources	9
Helixⁱ	52 – 57	Combined sources	6
Beta strandⁱ	68 – 74	Combined sources	7
Beta strandⁱ	77 – 83	Combined sources	7
Helixⁱ	90 – 94	Combined sources	5
Helixⁱ	97 – 101	Combined sources	5
Helixⁱ	104 – 123	Combined sources	20
Beta strandⁱ	125 – 127	Combined sources	3
Helixⁱ	136 – 138	Combined sources	3
Beta strandⁱ	139 – 141	Combined sources	3
Beta strandⁱ	147 – 149	Combined sources	3
Helixⁱ	154 – 157	Combined sources	4
Helixⁱ	159 – 162	Combined sources	4
Turnⁱ	167 – 169	Combined sources	3
Helixⁱ	170 – 172	Combined sources	3
Helixⁱ	175 – 178	Combined sources	4
Helixⁱ	186 – 201	Combined sources	16
Turnⁱ	205 – 208	Combined sources	4
Helixⁱ	211 – 220	Combined sources	10
Helixⁱ	233 – 242	Combined sources	10
Helixⁱ	247 – 249	Combined sources	3
Helixⁱ	253 – 264	Combined sources	12
Helixⁱ	269 – 277	Combined sources	9
Helixⁱ	280 – 298	Combined sources	19

Show more details

3D structure databases

ProteinModelPortalⁱ	Q9NYL2
SMRⁱ	Q9NYL2
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	16 – 277	Protein kinasePROSITE-ProRule annotationAdd BLAST		262
Domainⁱ	339 – 410	SAMPROSITE-ProRule annotationAdd BLAST		72

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	287 – 308	Leucine-zipperAdd BLAST		22

Sequence similaritiesⁱ

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.Curated

Phylogenomic databases

eggNOGⁱ	KOG0192 Eukaryota COG0515 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00900000140790
HOVERGENⁱ	HBG080445
InParanoidⁱ	Q9NYL2
KEGG Orthology (KO) More...KOⁱ	K04424
OMAⁱ	QWRCEIE
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G037E
PhylomeDBⁱ	Q9NYL2

Family and domain databases

InterProⁱ	View protein in InterPro IPR011009 Kinase-like_dom_sf IPR032938 MLTK IPR000719 Prot_kinase_dom IPR001660 SAM IPR013761 SAM/pointed_sf IPR001245 Ser-Thr/Tyr_kinase_cat_dom IPR008271 Ser/Thr_kinase_AS
PANTHERⁱ	PTHR44496 PTHR44496, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07714 Pkinase_Tyr, 1 hit PF00536 SAM_1, 1 hit
PRINTSⁱ	PR00109 TYRKINASE
SMARTⁱ	View protein in SMART SM00220 S_TKc, 1 hit SM00454 SAM, 1 hit
SUPFAMⁱ	SSF47769 SSF47769, 1 hit SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit PS50105 SAM_DOMAIN, 1 hit

Sequences (3+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show all Align All

Isoform 11 Publication (identifier: Q9NYL2-1) [UniParc]FASTA Add to basket

Also known as: Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSLGASFVQ IKFDDLQFFE NCGGGSFGSV YRAKWISQDK EVAVKKLLKI 
        60         70         80         90        100
EKEAEILSVL SHRNIIQFYG VILEPPNYGI VTEYASLGSL YDYINSNRSE 
       110        120        130        140        150
EMDMDHIMTW ATDVAKGMHY LHMEAPVKVI HRDLKSRNVV IAADGVLKIC 
       160        170        180        190        200
DFGASRFHNH TTHMSLVGTF PWMAPEVIQS LPVSETCDTY SYGVVLWEML 
       210        220        230        240        250
TREVPFKGLE GLQVAWLVVE KNERLTIPSS CPRSFAELLH QCWEADAKKR 
       260        270        280        290        300
PSFKQIISIL ESMSNDTSLP DKCNSFLHNK AEWRCEIEAT LERLKKLERD 
       310        320        330        340        350
LSFKEQELKE RERRLKMWEQ KLTEQSNTPL LPSFEIGAWT EDDVYCWVQQ 
       360        370        380        390        400
LVRKGDSSAE MSVYASLFKE NNITGKRLLL LEEEDLKDMG IVSKGHIIHF 
       410        420        430        440        450
KSAIEKLTHD YINLFHFPPL IKDSGGEPEE NEEKIVNLEL VFGFHLKPGT 
       460        470        480        490        500
GPQDCKWKMY MEMDGDEIAI TYIKDVTFNT NLPDAEILKM TKPPFVMEKW 
       510        520        530        540        550
IVGIAKSQTV ECTVTYESDV RTPKSTKHVH SIQWSRTKPQ DEVKAVQLAI 
       560        570        580        590        600
QTLFTNSDGN PGSRSDSSAD CQWLDTLRMR QIASNTSLQR SQSNPILGSP 
       610        620        630        640        650
FFSHFDGQDS YAAAVRRPQV PIKYQQITPV NQSRSSSPTQ YGLTKNFSSL 
       660        670        680        690        700
HLNSRDSGFS SGNTDTSSER GRYSDRSRNK YGRGSISLNS SPRGRYSGKS 
       710        720        730        740        750
QHSTPSRGRY PGKFYRVSQS ALNPHQSPDF KRSPRDLHQP NTIPGMPLHP 
       760        770        780        790        800
ETDSRASEED SKVSEGGWTK VEYRKKPHRP SPAKTNKERA RGDHRGWRNF

Length:800

Mass (Da):91,155

Last modified:November 30, 2010 - v3

Checksum:ⁱB2814509EC54B07A

GO

Isoform 21 Publication (identifier: Q9NYL2-2) [UniParc]FASTA Add to basket

Also known as: Beta

The sequence of this isoform differs from the canonical sequence as follows:
332-455: PSFEIGAWTE...LKPGTGPQDC → LPLAARMSEE...EEDNDMDNSE
456-800: Missing.

« Hide

        10         20         30         40         50
MSSLGASFVQ IKFDDLQFFE NCGGGSFGSV YRAKWISQDK EVAVKKLLKI 
        60         70         80         90        100
EKEAEILSVL SHRNIIQFYG VILEPPNYGI VTEYASLGSL YDYINSNRSE 
       110        120        130        140        150
EMDMDHIMTW ATDVAKGMHY LHMEAPVKVI HRDLKSRNVV IAADGVLKIC 
       160        170        180        190        200
DFGASRFHNH TTHMSLVGTF PWMAPEVIQS LPVSETCDTY SYGVVLWEML 
       210        220        230        240        250
TREVPFKGLE GLQVAWLVVE KNERLTIPSS CPRSFAELLH QCWEADAKKR 
       260        270        280        290        300
PSFKQIISIL ESMSNDTSLP DKCNSFLHNK AEWRCEIEAT LERLKKLERD 
       310        320        330        340        350
LSFKEQELKE RERRLKMWEQ KLTEQSNTPL LLPLAARMSE ESYFESKTEE 
       360        370        380        390        400
SNSAEMSCQI TATSNGEGHG MNPSLQAMML MGFGDIFSMN KAGAVMHSGM 
       410        420        430        440        450
QINMQAKQNS SKTTSKRRGK KVNMALGFSD FDLSEGDDDD DDDGEEEDND 

MDNSE

Show »

Length:455

Mass (Da):51,582

Checksum:ⁱE87DB84A4D58B752

GO

Isoform 3 (identifier: Q9NYL2-3) [UniParc]FASTA Add to basket

Also known as: HCCS-4

The sequence of this isoform differs from the canonical sequence as follows:
285-312: CEIEATLERLKKLERDLSFKEQELKERE → WVAPTAGHSVWLSKTITRLNEEVNQRSE
313-800: Missing.

« Hide

        10         20         30         40         50
MSSLGASFVQ IKFDDLQFFE NCGGGSFGSV YRAKWISQDK EVAVKKLLKI 
        60         70         80         90        100
EKEAEILSVL SHRNIIQFYG VILEPPNYGI VTEYASLGSL YDYINSNRSE 
       110        120        130        140        150
EMDMDHIMTW ATDVAKGMHY LHMEAPVKVI HRDLKSRNVV IAADGVLKIC 
       160        170        180        190        200
DFGASRFHNH TTHMSLVGTF PWMAPEVIQS LPVSETCDTY SYGVVLWEML 
       210        220        230        240        250
TREVPFKGLE GLQVAWLVVE KNERLTIPSS CPRSFAELLH QCWEADAKKR 
       260        270        280        290        300
PSFKQIISIL ESMSNDTSLP DKCNSFLHNK AEWRWVAPTA GHSVWLSKTI 
       310 
TRLNEEVNQR SE

Show »

Length:312

Mass (Da):35,468

Checksum:ⁱB655E873246339FB

GO

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

D4Q8H0	D4Q8H0_HUMAN	Mitogen-activated protein kinase ki... Mitogen-activated protein kinase kinase kinase MLT (Protein kinase)	pk ZAK	455	Annotation score:
C9J3F7	C9J3F7_HUMAN	Mitogen-activated protein kinase ki... Mitogen-activated protein kinase kinase kinase 20	MAP3K20	138	Annotation score:

Sequence cautionⁱ

The sequence BAD92211 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	346	C → W in AAF63490 (PubMed:10924358).Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_080563	95 – 800	Missing in CNM6; decrease of protein abundance. 1 PublicationAdd BLAST	706
Natural variantⁱVAR_080564	172 – 800	Missing in CNM6; decrease of protein abundance. 1 PublicationAdd BLAST	629
Natural variantⁱVAR_040806	267	T → M1 PublicationCorresponds to variant dbSNP:rs6758025Ensembl.	1
Natural variantⁱVAR_040807	281	A → T in an ovarian endometrioid sample; somatic mutation. 1 Publication	1
Natural variantⁱVAR_040808	281	A → V1 PublicationCorresponds to variant dbSNP:rs34683477Ensembl.	1
Natural variantⁱVAR_076448	368	F → C in SFMMP; produces protein aggregation. 1 PublicationCorresponds to variant dbSNP:rs863225437EnsemblClinVar.	1
Natural variantⁱVAR_022827	531	S → L5 PublicationsCorresponds to variant dbSNP:rs3769148Ensembl.	1
Natural variantⁱVAR_040809	580	R → W1 PublicationCorresponds to variant dbSNP:rs7593622Ensembl.	1
Natural variantⁱVAR_040810	740	P → T1 PublicationCorresponds to variant dbSNP:rs56202258Ensembl.	1
Natural variantⁱVAR_040811	773	Y → H1 PublicationCorresponds to variant dbSNP:rs35608243Ensembl.	1
Natural variantⁱVAR_040812	784	K → T2 PublicationsCorresponds to variant dbSNP:rs55830025Ensembl.	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_051741	285 – 312	CEIEA…LKERE → WVAPTAGHSVWLSKTITRLN EEVNQRSE in isoform 3. 1 PublicationAdd BLAST	28
Alternative sequenceⁱVSP_051742	313 – 800	Missing in isoform 3. 1 PublicationAdd BLAST	488
Alternative sequenceⁱVSP_051743	332 – 455	PSFEI…GPQDC → LPLAARMSEESYFESKTEES NSAEMSCQITATSNGEGHGM NPSLQAMMLMGFGDIFSMNK AGAVMHSGMQINMQAKQNSS KTTSKRRGKKVNMALGFSDF DLSEGDDDDDDDGEEEDNDM DNSE in isoform 2. 5 PublicationsAdd BLAST	124
Alternative sequenceⁱVSP_051744	456 – 800	Missing in isoform 2. 5 PublicationsAdd BLAST	345

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF238255 mRNA Translation: AAF63490.1 AB049733 mRNA Translation: BAB16444.1 AB049734 mRNA Translation: BAB16445.1 AF325454 mRNA Translation: AAK11615.1 AF480461 mRNA Translation: AAL85891.1 AF480462 mRNA Translation: AAL85892.1 AB030034 mRNA Translation: BAB12040.1 AF251441 mRNA Translation: AAF65822.1 AF465843 mRNA Translation: AAO33376.1 AK056310 mRNA Translation: BAG51674.1 AB208974 mRNA Translation: BAD92211.1 Different initiation. AC092573 Genomic DNA Translation: AAX82002.1 AC013461 Genomic DNA Translation: AAX93067.1 AC019046 Genomic DNA No translation available. CH471058 Genomic DNA Translation: EAX11164.1 BC001401 mRNA Translation: AAH01401.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS2251.1 [Q9NYL2-2] CCDS42777.1 [Q9NYL2-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_057737.2, NM_016653.2 [Q9NYL2-1] NP_598407.1, NM_133646.2 [Q9NYL2-2] XP_005246697.1, XM_005246640.2 [Q9NYL2-1] XP_016859812.1, XM_017004323.1 [Q9NYL2-2] XP_016859813.1, XM_017004324.1 [Q9NYL2-2]
UniGeneⁱ	Hs.444451

Genome annotation databases

Ensemblⁱ	ENST00000338983; ENSP00000340257; ENSG00000091436 [Q9NYL2-2] ENST00000375213; ENSP00000364361; ENSG00000091436 [Q9NYL2-1] ENST00000409176; ENSP00000387259; ENSG00000091436 [Q9NYL2-1] ENST00000539448; ENSP00000439414; ENSG00000091436 [Q9NYL2-2]
GeneIDⁱ	51776
KEGGⁱ	hsa:51776
UCSC genome browser More...UCSCⁱ	uc002uhz.4 human [Q9NYL2-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9NYL2	mitogen-activated protein kinase kinase kinase 20 isoform X1		Yangtze finless porpoise		940	UniRef90_Q9NYL2
Uncharacterized protein (Fragment)		AILME		803
Mixed lineage kinase-related kinase MRK-beta isoform 2 (Fragment)		MUSPF		306
Uncharacterized protein		SHEEP		802
Uncharacterized protein		BOVIN		802
+104
Q9NYL2-2	Uncharacterized protein		MOUSE		454	UniRef90_Q3TRG2
Uncharacterized protein		MACNE		563
Uncharacterized protein		RHIBE		483
Uncharacterized protein		AOTNA		461
Mitogen-activated protein kinase kinase kinase 20		PIG		459
+100

Protein	Similar proteins		Species	Score	Length	Source
Q9NYL2	mitogen-activated protein kinase kinase kinase 20 isoform X1		Yangtze finless porpoise		940	UniRef50_Q9NYL2
Uncharacterized protein (Fragment)		AILME		803
Mixed lineage kinase-related kinase MRK-beta isoform 2 (Fragment)		MUSPF		306
Uncharacterized protein		BOVIN		802
Uncharacterized protein		SHEEP		802
+277
Q9NYL2-2	Uncharacterized protein		MOUSE		454	UniRef50_Q3TRG2
Uncharacterized protein		MACNE		563
Uncharacterized protein		RHIBE		483
Uncharacterized protein		AOTNA		461
Uncharacterized protein		Cebus capucinus imitator		459
+162
Q9NYL2-3	Mitogen-activated protein kinase kinase kinase 20		ANAPL		289	UniRef50_Q9NYL2-3
Isoform 3 of Mitogen-activated protein kinase kinase kinase 20		MOUSE		289
Sterile alpha motif and leucine zipper containing kinase AZK		TAKRU		345
Uncharacterized protein		Micrurus lemniscatus lemniscatus		147
Uncharacterized protein		ONCMY		306
+20

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF238255 mRNA Translation: AAF63490.1 AB049733 mRNA Translation: BAB16444.1 AB049734 mRNA Translation: BAB16445.1 AF325454 mRNA Translation: AAK11615.1 AF480461 mRNA Translation: AAL85891.1 AF480462 mRNA Translation: AAL85892.1 AB030034 mRNA Translation: BAB12040.1 AF251441 mRNA Translation: AAF65822.1 AF465843 mRNA Translation: AAO33376.1 AK056310 mRNA Translation: BAG51674.1 AB208974 mRNA Translation: BAD92211.1 Different initiation. AC092573 Genomic DNA Translation: AAX82002.1 AC013461 Genomic DNA Translation: AAX93067.1 AC019046 Genomic DNA No translation available. CH471058 Genomic DNA Translation: EAX11164.1 BC001401 mRNA Translation: AAH01401.1
CCDSⁱ	CCDS2251.1 [Q9NYL2-2] CCDS42777.1 [Q9NYL2-1]
RefSeqⁱ	NP_057737.2, NM_016653.2 [Q9NYL2-1] NP_598407.1, NM_133646.2 [Q9NYL2-2] XP_005246697.1, XM_005246640.2 [Q9NYL2-1] XP_016859812.1, XM_017004323.1 [Q9NYL2-2] XP_016859813.1, XM_017004324.1 [Q9NYL2-2]
UniGeneⁱ	Hs.444451

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	5HES	X-ray	2.14	A/B	5-309	[»]
	5X5O	X-ray	1.87	A	5-309	[»]
ProteinModelPortalⁱ	Q9NYL2
SMRⁱ	Q9NYL2
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	119725, 44 interactors
IntActⁱ	Q9NYL2, 40 interactors
MINTⁱ	Q9NYL2
STRINGⁱ	9606.ENSP00000364361

Chemistry databases

BindingDBⁱ	Q9NYL2
ChEMBLⁱ	CHEMBL3886
GuidetoPHARMACOLOGYⁱ	2289

PTM databases

iPTMnetⁱ	Q9NYL2
PhosphoSitePlusⁱ	Q9NYL2

Polymorphism and mutation databases

BioMutaⁱ	MLTK
DMDMⁱ	313104215

Proteomic databases

EPDⁱ	Q9NYL2
MaxQBⁱ	Q9NYL2
PaxDbⁱ	Q9NYL2
PeptideAtlasⁱ	Q9NYL2
PRIDEⁱ	Q9NYL2
ProteomicsDBⁱ	83246 83247 [Q9NYL2-2] 83248 [Q9NYL2-3]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	51776
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000338983; ENSP00000340257; ENSG00000091436 [Q9NYL2-2] ENST00000375213; ENSP00000364361; ENSG00000091436 [Q9NYL2-1] ENST00000409176; ENSP00000387259; ENSG00000091436 [Q9NYL2-1] ENST00000539448; ENSP00000439414; ENSG00000091436 [Q9NYL2-2]
GeneIDⁱ	51776
KEGGⁱ	hsa:51776
UCSCⁱ	uc002uhz.4 human [Q9NYL2-1]

Organism-specific databases

CTDⁱ	51776
DisGeNETⁱ	51776
EuPathDBⁱ	HostDB:ENSG00000091436.16
GeneCardsⁱ	MAP3K20
H-InvDBⁱ	HIX0030332
HGNCⁱ	HGNC:17797 MAP3K20
HPAⁱ	HPA017205
MalaCardsⁱ	MAP3K20
MIMⁱ	609479 gene 616890 phenotype 617760 phenotype
neXtProtⁱ	NX_Q9NYL2
OpenTargetsⁱ	ENSG00000091436
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0192 Eukaryota COG0515 LUCA
GeneTreeⁱ	ENSGT00900000140790
HOVERGENⁱ	HBG080445
InParanoidⁱ	Q9NYL2
KOⁱ	K04424
OMAⁱ	QWRCEIE
OrthoDBⁱ	EOG091G037E
PhylomeDBⁱ	Q9NYL2

Enzyme and pathway databases

SignaLinkⁱ	Q9NYL2
SIGNORⁱ	Q9NYL2

Miscellaneous databases

ChiTaRSⁱ	ZAK human
GeneWikiⁱ	ZAK
GenomeRNAiⁱ	51776
Protein Ontology More...PROⁱ	PR:Q9NYL2
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000091436 Expressed in 221 organ(s), highest expression level in heart left ventricle
ExpressionAtlasⁱ	Q9NYL2 baseline and differential
Genevisibleⁱ	Q9NYL2 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR011009 Kinase-like_dom_sf IPR032938 MLTK IPR000719 Prot_kinase_dom IPR001660 SAM IPR013761 SAM/pointed_sf IPR001245 Ser-Thr/Tyr_kinase_cat_dom IPR008271 Ser/Thr_kinase_AS
PANTHERⁱ	PTHR44496 PTHR44496, 1 hit
Pfamⁱ	View protein in Pfam PF07714 Pkinase_Tyr, 1 hit PF00536 SAM_1, 1 hit
PRINTSⁱ	PR00109 TYRKINASE
SMARTⁱ	View protein in SMART SM00220 S_TKc, 1 hit SM00454 SAM, 1 hit
SUPFAMⁱ	SSF47769 SSF47769, 1 hit SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit PS50105 SAM_DOMAIN, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	M3K20_HUMAN
Accessionⁱ	Q9NYL2Primary (citable) accession number: Q9NYL2 Secondary accession number(s): B3KPG2 Q9NYE9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
	Last sequence update:	November 30, 2010
	Last modified:	September 12, 2018
	This is version 165 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human and mouse protein kinases
Human and mouse protein kinases: classification and index
SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

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UniProtKB - Q9NYL2 (M3K20_HUMAN)

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Mitogen-activated protein kinase kinase kinase 20

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

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GO - Biological processi

Enzyme and pathway databases

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<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

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Amino acid modifications

Keywords - PTMi

Proteomic databases

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<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

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<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

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<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

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Sequence databases

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Keywords - Coding sequence diversityi

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Chemistry databases

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Gene expression databases

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<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ