Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Potassium channel subfamily K member 4

Gene

KCNK4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Voltage-insensitive potassium channel (PubMed:22282805). Channel opening is triggered by mechanical forces that deform the membrane (PubMed:22282805, PubMed:25471887, PubMed:25500157). Channel opening is triggered by raising the intracellular pH to basic levels (By similarity). The channel is inactive at 24 degrees Celsius (in vitro); raising the temperature to 37 degrees Celsius increases the frequency of channel opening, with a further increase in channel activity when the temperature is raised to 42 degrees Celsius (By similarity). Plays a role in the perception of pain caused by heat (By similarity). Plays a role in the sensory perception of pain caused by pressure (By similarity).By similarity3 Publications

Activity regulationi

Activated by arachidonic acid.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Potassium channel, Voltage-gated channel
Biological processIon transport, Potassium transport, Transport
LigandPotassium

Enzyme and pathway databases

ReactomeiR-HSA-1299503 TWIK related potassium channel (TREK)
R-HSA-5576886 Phase 4 - resting membrane potential

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel subfamily K member 4
Alternative name(s):
TWIK-related arachidonic acid-stimulated potassium channel protein1 Publication
Short name:
TRAAK1 Publication
Two pore potassium channel KT4.1
Short name:
Two pore K(+) channel KT4.1
Gene namesi
Name:KCNK4
Synonyms:TRAAK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000182450.12
HGNCiHGNC:6279 KCNK4
MIMi605720 gene
neXtProtiNX_Q9NYG8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3Cytoplasmic4 Publications3
Transmembranei4 – 24Helical4 PublicationsAdd BLAST21
Topological domaini25 – 87Extracellular4 PublicationsAdd BLAST63
Intramembranei88 – 102Helical; Name=Pore helix 14 PublicationsAdd BLAST15
Intramembranei103 – 1094 Publications7
Topological domaini110 – 117Extracellular4 Publications8
Transmembranei118 – 150Helical4 PublicationsAdd BLAST33
Topological domaini151 – 172Cytoplasmic4 PublicationsAdd BLAST22
Transmembranei173 – 194Helical4 PublicationsAdd BLAST22
Topological domaini195 – 199Extracellular4 Publications5
Intramembranei200 – 213Helical; Name=Pore helix 24 PublicationsAdd BLAST14
Intramembranei214 – 2194 Publications6
Topological domaini220 – 233Extracellular4 PublicationsAdd BLAST14
Transmembranei234 – 260Helical4 PublicationsAdd BLAST27
Topological domaini261 – 393Cytoplasmic4 PublicationsAdd BLAST133

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi98G → I: Strongly increases basal level of channel activity, decreases further activation by pressure and abolishes further activation by arachidonic acid. 1 Publication1
Mutagenesisi236W → S: Increases basal level of channel activity and decreases further activation by pressure, but has little effect on further activation by arachidonic acid. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000182450
PharmGKBiPA30061

Chemistry databases

ChEMBLiCHEMBL3714486
GuidetoPHARMACOLOGYi516

Polymorphism and mutation databases

BioMutaiKCNK4
DMDMi13124080

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001017471 – 393Potassium channel subfamily K member 4Add BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi52Interchain4 Publications
Glycosylationi78N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi82N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9NYG8
PeptideAtlasiQ9NYG8
PRIDEiQ9NYG8
ProteomicsDBi83229
83230 [Q9NYG8-2]

PTM databases

iPTMnetiQ9NYG8
PhosphoSitePlusiQ9NYG8

Expressioni

Gene expression databases

BgeeiENSG00000182450 Expressed in 38 organ(s), highest expression level in amygdala
CleanExiHS_KCNK4
ExpressionAtlasiQ9NYG8 baseline and differential
GenevisibleiQ9NYG8 HS

Interactioni

Subunit structurei

Homodimer; disulfide-linked.4 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi119122, 2 interactors
DIPiDIP-61343N
IntActiQ9NYG8, 3 interactors
STRINGi9606.ENSP00000378033

Chemistry databases

BindingDBiQ9NYG8

Structurei

Secondary structure

1393
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9NYG8
SMRiQ9NYG8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni103 – 108Selectivity filter 14 Publications6
Regioni212 – 217Selectivity filter 24 Publications6

Domaini

Channel opening is brought about by a conformation change that involves buckling of the second transmembrane helix and affects the position and orientation of the fourth transmembrane helix.2 Publications

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1418 Eukaryota
COG1226 LUCA
GeneTreeiENSGT00760000118858
HOGENOMiHOG000013106
HOVERGENiHBG052234
InParanoidiQ9NYG8
KOiK04915
OMAiLDYPSEN
OrthoDBiEOG091G0E3R
PhylomeDBiQ9NYG8
TreeFamiTF313947

Family and domain databases

InterProiView protein in InterPro
IPR003280 2pore_dom_K_chnl
IPR008074 2pore_dom_K_chnl_TRAAK
IPR013099 K_chnl_dom
PfamiView protein in Pfam
PF07885 Ion_trans_2, 2 hits
PRINTSiPR01333 2POREKCHANEL
PR01691 TRAAKCHANNEL

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: Q9NYG8-1) [UniParc]FASTAAdd to basket
Also known as: KT4.1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRSTTLLALL ALVLLYLVSG ALVFRALEQP HEQQAQRELG EVREKFLRAH
60 70 80 90 100
PCVSDQELGL LIKEVADALG GGADPETNST SNSSHSAWDL GSAFFFSGTI
110 120 130 140 150
ITTIGYGNVA LRTDAGRLFC IFYALVGIPL FGILLAGVGD RLGSSLRHGI
160 170 180 190 200
GHIEAIFLKW HVPPELVRVL SAMLFLLIGC LLFVLTPTFV FCYMEDWSKL
210 220 230 240 250
EAIYFVIVTL TTVGFGDYVA GADPRQDSPA YQPLVWFWIL LGLAYFASVL
260 270 280 290 300
TTIGNWLRVV SRRTRAEMGG LTAQAASWTG TVTARVTQRA GPAAPPPEKE
310 320 330 340 350
QPLLPPPPCP AQPLGRPRSP SPPEKAQPPS PPTASALDYP SENLAFIDES
360 370 380 390
SDTQSERGCP LPRAPRGRRR PNPPRKPVRP RGPGRPRDKG VPV
Length:393
Mass (Da):42,704
Last modified:February 21, 2001 - v2
Checksum:i7F18E53A0A9AD57D
GO
Isoform 2 (identifier: Q9NYG8-2) [UniParc]FASTAAdd to basket
Also known as: KT4.1b

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MTTAPQEPPARPLQAGSGAGPAPGRAM

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:419
Mass (Da):45,173
Checksum:iE7DEDE17B30C0FBF
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F5GZ20F5GZ20_HUMAN
Potassium channel subfamily K membe...
KCNK4
158Annotation score:
F5GYE0F5GYE0_HUMAN
Potassium channel subfamily K membe...
KCNK4
310Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_020206328P → L1 PublicationCorresponds to variant dbSNP:rs953778Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0066891M → MTTAPQEPPARPLQAGSGAG PAPGRAM in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF248242 mRNA Translation: AAG31731.1
AF247042 mRNA Translation: AAF64062.1
AF259500 mRNA Translation: AAK49389.1
AF259501 mRNA Translation: AAK49390.1
EU978935 mRNA Translation: ACH86094.1
CH471076 Genomic DNA Translation: EAW74242.1
CCDSiCCDS8067.1 [Q9NYG8-1]
RefSeqiNP_001304019.1, NM_001317090.1 [Q9NYG8-1]
NP_201567.1, NM_033310.2 [Q9NYG8-1]
UniGeneiHs.647233

Genome annotation databases

EnsembliENST00000394525; ENSP00000378033; ENSG00000182450 [Q9NYG8-1]
ENST00000422670; ENSP00000402797; ENSG00000182450 [Q9NYG8-1]
ENST00000539216; ENSP00000444948; ENSG00000182450 [Q9NYG8-1]
GeneIDi50801
KEGGihsa:50801
UCSCiuc001nzj.2 human [Q9NYG8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiKCNK4_HUMAN
AccessioniPrimary (citable) accession number: Q9NYG8
Secondary accession number(s): B5TJL1, Q96T94
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 21, 2001
Last modified: September 12, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again