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Entry version 184 (25 May 2022)
Sequence version 1 (01 Oct 2000)
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Protein

Sphingosine kinase 1

Gene

SPHK1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol (PubMed:20577214, PubMed:23602659, PubMed:29662056, PubMed:24929359, PubMed:11923095).

In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis (PubMed:16118219).

Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling leading to IL17 secretion (PubMed:20577214).

In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway (PubMed:23935096).

Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity (PubMed:28049734, PubMed:24929359).

In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA (PubMed:25417698).

9 Publications

Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia.

By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Acetyltransferase activity increases in presence of the kinase substrate, sphingosine (By similarity). In Purkinje cells, kinase activity on sphingosine increases in presence of VEGFA (PubMed:25417698). In neurons, kinase activity increases during the first 24h in presence of Amyloid-beta protein 42 to decrease after 96h (By similarity).By similarity1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 124 sec(-1) for sphingosine and increases to 304 sec(-1) upon interaction with EEF1A1.1 Publication
  1. KM=14 µM for sphingosine1 Publication
  2. KM=20 µM for dihydrosphingosine1 Publication
  3. KM=77 µM for ATP1 Publication
  4. KM=3.8 µM for 1-O-hexadecyl-2-amino-sn-glycerol1 Publication
  5. KM=15.7 µM for sphing-4-enine1 Publication
  6. KM=46 µM for sphinganine1 Publication
  7. KM=24 µM for sphingosine1 Publication
  8. KM=89 µM for ATP1 Publication

pH dependencei

Optimum pH is 7.4.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei81Proton donor/acceptor1 Publication1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei86ATP1 Publication1
Binding sitei178Substrate1 Publication1
Binding sitei185ATP1 Publication1
Binding sitei191ATP1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi22 – 24ATP1 Publication3
Nucleotide bindingi54 – 58ATP1 Publication5
Nucleotide bindingi111 – 113ATP1 Publication3
Nucleotide bindingi341 – 343ATP1 Publication3

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionCalmodulin-binding, Kinase, Transferase
Biological processLipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

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BRENDAi
2.7.1.91, 2681

Pathway Commons web resource for biological pathway data

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PathwayCommonsi
Q9NYA1

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-HSA-1660661, Sphingolipid de novo biosynthesis
R-HSA-390471, Association of TriC/CCT with target proteins during biosynthesis
R-HSA-5218921, VEGFR2 mediated cell proliferation
R-HSA-9009391, Extra-nuclear estrogen signaling

SABIO-RK: Biochemical Reaction Kinetics Database

More...
SABIO-RKi
Q9NYA1

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...
SignaLinki
Q9NYA1

SIGNOR Signaling Network Open Resource

More...
SIGNORi
Q9NYA1

Chemistry databases

SwissLipids knowledge resource for lipid biology

More...
SwissLipidsi
SLP:000000111

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Sphingosine kinase 1Curated (EC:2.7.1.914 Publications)
Short name:
SK 11 Publication
Short name:
SPK 1
Alternative name(s):
Acetyltransferase SPHK1 (EC:2.3.1.-)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:SPHK1Imported
Synonyms:SK11 Publication, SPHK, SPK
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 17

Organism-specific databases

Human Gene Nomenclature Database

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HGNCi
HGNC:11240, SPHK1

Online Mendelian Inheritance in Man (OMIM)

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MIMi
603730, gene

neXtProt; the human protein knowledge platform

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neXtProti
NX_Q9NYA1

Eukaryotic Pathogen, Vector and Host Database Resources

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VEuPathDBi
HostDB:ENSG00000176170

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cell junction, Cell membrane, Coated pit, Cytoplasm, Endosome, Membrane, Nucleus, Synapse

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi81D → A: Loss of enzyme activity. 1 Publication1
Mutagenesisi81D → N: Strongly reduced enzyme activity. 1 Publication1
Mutagenesisi82G → D: Loss of enzyme activity. 1 Publication1
Mutagenesisi194L → Q: Loss of binding to negatively charged membranes, diffuse cytosolic distribution. 1 Publication1
Mutagenesisi197 – 198FL → AA: Abolishes interaction with CIB1. 1 Publication2
Mutagenesisi197 – 198FL → AQ: Loss of binding to negatively charged membranes, diffuse cytosolic distribution. 1 Publication2

Organism-specific databases

DisGeNET

More...
DisGeNETi
8877

Open Targets

More...
OpenTargetsi
ENSG00000176170

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...
PharmGKBi
PA36070

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

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Pharosi
Q9NYA1, Tchem

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...
ChEMBLi
CHEMBL4394

Drug and drug target database

More...
DrugBanki
DB08868, Fingolimod

IUPHAR/BPS Guide to PHARMACOLOGY

More...
GuidetoPHARMACOLOGYi
2204

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...
BioMutai
SPHK1

Domain mapping of disease mutations (DMDM)

More...
DMDMi
17369329

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001813571 – 384Sphingosine kinase 1Add BLAST384

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei193PhosphothreonineCombined sources1
Modified residuei225Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

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EPDi
Q9NYA1

jPOST - Japan Proteome Standard Repository/Database

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jPOSTi
Q9NYA1

MassIVE - Mass Spectrometry Interactive Virtual Environment

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MassIVEi
Q9NYA1

MaxQB - The MaxQuant DataBase

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MaxQBi
Q9NYA1

PaxDb, a database of protein abundance averages across all three domains of life

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PaxDbi
Q9NYA1

PeptideAtlas

More...
PeptideAtlasi
Q9NYA1

PRoteomics IDEntifications database

More...
PRIDEi
Q9NYA1

ProteomicsDB: a multi-organism proteome resource

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ProteomicsDBi
83202 [Q9NYA1-1]
83203 [Q9NYA1-2]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q9NYA1

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
Q9NYA1

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Expressed in brain cortex (at protein level) (PubMed:29662056).2 Publications

Gene expression databases

Bgee dataBase for Gene Expression Evolution

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Bgeei
ENSG00000176170, Expressed in stromal cell of endometrium and 168 other tissues

ExpressionAtlas, Differential and Baseline Expression

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ExpressionAtlasi
Q9NYA1, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
Q9NYA1, HS

Organism-specific databases

Human Protein Atlas

More...
HPAi
ENSG00000176170, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with ACY1 (By similarity). Binds to calmodulin.

Interacts with SPHKAP (PubMed:12080051).

Interacts with CIB1, the interaction occurs in a calcium-dependent manner (PubMed:19854831).

Interacts with TRAF2 (PubMed:20577214).

Interacts with EEF1A1; the interaction enhances SPHK1 kinase activity (PubMed:18263879).

By similarity4 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

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BioGRIDi
114396, 51 interactors

Protein interaction database and analysis system

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IntActi
Q9NYA1, 60 interactors

Molecular INTeraction database

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MINTi
Q9NYA1

STRING: functional protein association networks

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STRINGi
9606.ENSP00000313681

Chemistry databases

BindingDB database of measured binding affinities

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BindingDBi
Q9NYA1

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

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RNActi
Q9NYA1, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1384
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

AlphaFold Protein Structure Database

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AlphaFoldDBi
Q9NYA1

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q9NYA1

Database of comparative protein structure models

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ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini12 – 159DAGKcPROSITE-ProRule annotationAdd BLAST148

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni79 – 82Substrate binding1 Publication4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi147 – 155Nuclear export signal 11 Publication9
Motifi161 – 169Nuclear export signal 21 Publication9

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
KOG1116, Eukaryota

Ensembl GeneTree

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GeneTreei
ENSGT00940000157864

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

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HOGENOMi
CLU_013399_1_0_1

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q9NYA1

Identification of Orthologs from Complete Genome Data

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OMAi
AECDLGT

Database of Orthologous Groups

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OrthoDBi
681139at2759

Database for complete collections of gene phylogenies

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PhylomeDBi
Q9NYA1

TreeFam database of animal gene trees

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TreeFami
TF354296

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
3.40.50.10330, 1 hit

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR017438, ATP-NAD_kinase_N
IPR001206, Diacylglycerol_kinase_cat_dom
IPR016064, NAD/diacylglycerol_kinase_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00781, DAGK_cat, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

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SMARTi
View protein in SMART
SM00046, DAGKc, 1 hit

Superfamily database of structural and functional annotation

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SUPFAMi
SSF111331, SSF111331, 1 hit

PROSITE; a protein domain and family database

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PROSITEi
View protein in PROSITE
PS50146, DAGK, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 3 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9NYA1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <p><strong>What is the canonical sequence?</strong><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDPAGGPRGV LPRPCRVLVL LNPRGGKGKA LQLFRSHVQP LLAEAEISFT
60 70 80 90 100
LMLTERRNHA RELVRSEELG RWDALVVMSG DGLMHEVVNG LMERPDWETA
110 120 130 140 150
IQKPLCSLPA GSGNALAASL NHYAGYEQVT NEDLLTNCTL LLCRRLLSPM
160 170 180 190 200
NLLSLHTASG LRLFSVLSLA WGFIADVDLE SEKYRRLGEM RFTLGTFLRL
210 220 230 240 250
AALRTYRGRL AYLPVGRVGS KTPASPVVVQ QGPVDAHLVP LEEPVPSHWT
260 270 280 290 300
VVPDEDFVLV LALLHSHLGS EMFAAPMGRC AAGVMHLFYV RAGVSRAMLL
310 320 330 340 350
RLFLAMEKGR HMEYECPYLV YVPVVAFRLE PKDGKGVFAV DGELMVSEAV
360 370 380
QGQVHPNYFW MVSGCVEPPP SWKPQQMPPP EEPL
Length:384
Mass (Da):42,518
Last modified:October 1, 2000 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iEB04A7F2034C2DB0
GO
Isoform 2 (identifier: Q9NYA1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MSAQVLGFLR...WQREPRVEVM

Show »
Length:470
Mass (Da):51,054
Checksum:iF6BF2D324E12CFC0
GO
Isoform 3 (identifier: Q9NYA1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     3-3: P → PVVGCGRGLFGFVFS

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Length:398
Mass (Da):43,944
Checksum:i7F2C9B26C030E560
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K7EJ32K7EJ32_HUMAN
Sphingosine kinase 1
SPHK1
111Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
K7EMA4K7EMA4_HUMAN
Sphingosine kinase 1
SPHK1
152Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti6Missing in CAB92131 (Ref. 4) Curated1
Sequence conflicti11 – 15LPRPC → ARL in CAB92131 (Ref. 4) Curated5
Sequence conflicti114 – 115NA → KP in CAB92131 (Ref. 4) Curated2
Sequence conflicti251V → M in AAF73423 (PubMed:10802064).Curated1
Sequence conflicti260V → I in AAF73423 (PubMed:10802064).Curated1
Sequence conflicti302L → F in AAF73423 (PubMed:10802064).Curated1
Sequence conflicti325V → G in CAB92131 (Ref. 4) Curated1
Sequence conflicti337V → M in AAG01980 (PubMed:10947957).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Isoform 2 (identifier: Q9NYA1-2)
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_08291934A → TCuratedCorresponds to variant dbSNP:rs346803Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0354531M → MSAQVLGFLRSWTPLPLAAP RGPAAAGNDAGAPAATAPGG EGEPHSRPCDARLGSTDKEL KAGAAATGSAPTAPGTPWQR EPRVEVM in isoform 2. 2 Publications1
Alternative sequenceiVSP_0470783P → PVVGCGRGLFGFVFS in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
AF266756 mRNA Translation: AAF73470.1
AF238083 mRNA Translation: AAF73423.1
AF200328 mRNA Translation: AAG01980.1
AK023393 mRNA Translation: BAB14558.1
AK292294 mRNA Translation: BAF84983.1
AK022402 mRNA Translation: BAB14028.1
AJ245504 mRNA Translation: CAB92131.1
AC090699 Genomic DNA No translation available.
CH471099 Genomic DNA Translation: EAW89392.1
CH471099 Genomic DNA Translation: EAW89393.1
BC009419 mRNA Translation: AAH09419.1
BC014439 mRNA Translation: AAH14439.1
BC030553 mRNA Translation: AAH30553.1

The Consensus CDS (CCDS) project

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CCDSi
CCDS11744.1 [Q9NYA1-2]
CCDS45785.1 [Q9NYA1-1]
CCDS59297.1 [Q9NYA1-3]

NCBI Reference Sequences

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RefSeqi
NP_001136073.1, NM_001142601.1 [Q9NYA1-1]
NP_001136074.1, NM_001142602.1 [Q9NYA1-1]
NP_068807.2, NM_021972.3 [Q9NYA1-3]
NP_892010.2, NM_182965.2 [Q9NYA1-2]
XP_005257823.1, XM_005257766.2

Genome annotation databases

Ensembl eukaryotic genome annotation project

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Ensembli
ENST00000323374.8; ENSP00000313681.3; ENSG00000176170.14 [Q9NYA1-2]
ENST00000392496.3; ENSP00000376285.2; ENSG00000176170.14
ENST00000545180.5; ENSP00000440970.1; ENSG00000176170.14
ENST00000590959.5; ENSP00000468547.1; ENSG00000176170.14 [Q9NYA1-3]
ENST00000592299.6; ENSP00000465726.2; ENSG00000176170.14

Database of genes from NCBI RefSeq genomes

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GeneIDi
8877

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
hsa:8877

Matched Annotation from NCBI and EMBL-EBI (MANE) - Phase one

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MANE-Selecti
ENST00000592299.6; ENSP00000465726.2; NM_001142601.2; NP_001136073.1

UCSC genome browser

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UCSCi
uc002jrf.1, human [Q9NYA1-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF266756 mRNA Translation: AAF73470.1
AF238083 mRNA Translation: AAF73423.1
AF200328 mRNA Translation: AAG01980.1
AK023393 mRNA Translation: BAB14558.1
AK292294 mRNA Translation: BAF84983.1
AK022402 mRNA Translation: BAB14028.1
AJ245504 mRNA Translation: CAB92131.1
AC090699 Genomic DNA No translation available.
CH471099 Genomic DNA Translation: EAW89392.1
CH471099 Genomic DNA Translation: EAW89393.1
BC009419 mRNA Translation: AAH09419.1
BC014439 mRNA Translation: AAH14439.1
BC030553 mRNA Translation: AAH30553.1
CCDSiCCDS11744.1 [Q9NYA1-2]
CCDS45785.1 [Q9NYA1-1]
CCDS59297.1 [Q9NYA1-3]
RefSeqiNP_001136073.1, NM_001142601.1 [Q9NYA1-1]
NP_001136074.1, NM_001142602.1 [Q9NYA1-1]
NP_068807.2, NM_021972.3 [Q9NYA1-3]
NP_892010.2, NM_182965.2 [Q9NYA1-2]
XP_005257823.1, XM_005257766.2

3D structure databases

Select the link destinations:

Protein Data Bank Europe

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PDBei

Protein Data Bank RCSB

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RCSB PDBi

Protein Data Bank Japan

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PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VZBX-ray2.00A/B/C9-364[»]
3VZCX-ray2.30A/B/C/D/E/F9-364[»]
3VZDX-ray2.30A/B/C/D/E/F9-364[»]
4L02X-ray2.75A/B/C9-364[»]
4V24X-ray1.80A/B1-363[»]
AlphaFoldDBiQ9NYA1
SMRiQ9NYA1
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi114396, 51 interactors
IntActiQ9NYA1, 60 interactors
MINTiQ9NYA1
STRINGi9606.ENSP00000313681

Chemistry databases

BindingDBiQ9NYA1
ChEMBLiCHEMBL4394
DrugBankiDB08868, Fingolimod
GuidetoPHARMACOLOGYi2204
SwissLipidsiSLP:000000111

PTM databases

iPTMnetiQ9NYA1
PhosphoSitePlusiQ9NYA1

Genetic variation databases

BioMutaiSPHK1
DMDMi17369329

Proteomic databases

EPDiQ9NYA1
jPOSTiQ9NYA1
MassIVEiQ9NYA1
MaxQBiQ9NYA1
PaxDbiQ9NYA1
PeptideAtlasiQ9NYA1
PRIDEiQ9NYA1
ProteomicsDBi83202 [Q9NYA1-1]
83203 [Q9NYA1-2]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

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Antibodypediai
19687, 737 antibodies from 47 providers

The DNASU plasmid repository

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DNASUi
8877

Genome annotation databases

EnsembliENST00000323374.8; ENSP00000313681.3; ENSG00000176170.14 [Q9NYA1-2]
ENST00000392496.3; ENSP00000376285.2; ENSG00000176170.14
ENST00000545180.5; ENSP00000440970.1; ENSG00000176170.14
ENST00000590959.5; ENSP00000468547.1; ENSG00000176170.14 [Q9NYA1-3]
ENST00000592299.6; ENSP00000465726.2; ENSG00000176170.14
GeneIDi8877
KEGGihsa:8877
MANE-SelectiENST00000592299.6; ENSP00000465726.2; NM_001142601.2; NP_001136073.1
UCSCiuc002jrf.1, human [Q9NYA1-1]

Organism-specific databases

Comparative Toxicogenomics Database

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CTDi
8877
DisGeNETi8877

GeneCards: human genes, protein and diseases

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GeneCardsi
SPHK1
HGNCiHGNC:11240, SPHK1
HPAiENSG00000176170, Low tissue specificity
MIMi603730, gene
neXtProtiNX_Q9NYA1
OpenTargetsiENSG00000176170
PharmGKBiPA36070
VEuPathDBiHostDB:ENSG00000176170

GenAtlas: human gene database

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GenAtlasi
Search...

Phylogenomic databases

eggNOGiKOG1116, Eukaryota
GeneTreeiENSGT00940000157864
HOGENOMiCLU_013399_1_0_1
InParanoidiQ9NYA1
OMAiAECDLGT
OrthoDBi681139at2759
PhylomeDBiQ9NYA1
TreeFamiTF354296

Enzyme and pathway databases

BRENDAi2.7.1.91, 2681
PathwayCommonsiQ9NYA1
ReactomeiR-HSA-1660661, Sphingolipid de novo biosynthesis
R-HSA-390471, Association of TriC/CCT with target proteins during biosynthesis
R-HSA-5218921, VEGFR2 mediated cell proliferation
R-HSA-9009391, Extra-nuclear estrogen signaling
SABIO-RKiQ9NYA1
SignaLinkiQ9NYA1
SIGNORiQ9NYA1

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

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BioGRID-ORCSi
8877, 15 hits in 1083 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

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ChiTaRSi
SPHK1, human

The Gene Wiki collection of pages on human genes and proteins

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GeneWikii
Sphingosine_kinase_1

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

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GenomeRNAii
8877
PharosiQ9NYA1, Tchem

Protein Ontology

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PROi
PR:Q9NYA1
RNActiQ9NYA1, protein

The Stanford Online Universal Resource for Clones and ESTs

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SOURCEi
Search...

Gene expression databases

BgeeiENSG00000176170, Expressed in stromal cell of endometrium and 168 other tissues
ExpressionAtlasiQ9NYA1, baseline and differential
GenevisibleiQ9NYA1, HS

Family and domain databases

Gene3Di3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438, ATP-NAD_kinase_N
IPR001206, Diacylglycerol_kinase_cat_dom
IPR016064, NAD/diacylglycerol_kinase_sf
PfamiView protein in Pfam
PF00781, DAGK_cat, 1 hit
SMARTiView protein in SMART
SM00046, DAGKc, 1 hit
SUPFAMiSSF111331, SSF111331, 1 hit
PROSITEiView protein in PROSITE
PS50146, DAGK, 1 hit

MobiDB: a database of protein disorder and mobility annotations

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MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSPHK1_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9NYA1
Secondary accession number(s): Q8N632
, Q96GK1, Q9HD92, Q9NY70, Q9NYL3
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: October 1, 2000
Last modified: May 25, 2022
This is version 184 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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