UniProtKB - Q9NY97 (B3GN2_HUMAN)
Protein
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2
Gene
B3GNT2
Organism
Homo sapiens (Human)
Status
Functioni
Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Catalyzes the initiation and elongation of poly-N-acetyllactosamine chains. Shows a marked preference for Gal(beta1-4)Glc(NAc)-based acceptors (PubMed:9892646). Probably constitutes the main polylactosamine synthase.3 Publications
Caution
Was indicated as B3Gal-T6 in submitted DNA entries.Curated
Catalytic activityi
- a β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosaminyl derivative + UDP-N-acetyl-α-D-glucosamine = an N-acetyl-β-D-glucosaminyl-(1→3)-β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosaminyl derivative + H+ + UDP3 PublicationsEC:2.4.1.1493 Publications
Cofactori
Mn2+1 Publication
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
- acetylgalactosaminyltransferase activity Source: GO_Central
- acetylglucosaminyltransferase activity Source: GO_Central
- beta-galactosyl-N-acetylglucosaminylgalactosylglucosyl-ceramide beta-1,3-acetylglucosaminyltransferase activity Source: UniProtKB-EC
- N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity Source: UniProtKB
GO - Biological processi
- axon guidance Source: Ensembl
- cellular response to leukemia inhibitory factor Source: Ensembl
- keratan sulfate biosynthetic process Source: Reactome
- O-glycan processing Source: Reactome
- poly-N-acetyllactosamine biosynthetic process Source: UniProtKB
- protein glycosylation Source: GO_Central
- sensory perception of smell Source: Ensembl
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Ligand | Manganese |
Enzyme and pathway databases
BioCyci | MetaCyc:ENSG00000170340-MONOMER |
PathwayCommonsi | Q9NY97 |
Reactomei | R-HSA-2022854, Keratan sulfate biosynthesis R-HSA-913709, O-linked glycosylation of mucins |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT31, Glycosyltransferase Family 31 |
Names & Taxonomyi
Protein namesi | Recommended name: N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2 (EC:2.4.1.1493 Publications)Alternative name(s): Beta-1,3-N-acetylglucosaminyltransferase 1 Short name: BGnT-1 Short name: Beta-1,3-Gn-T1 Short name: Beta3Gn-T1 Beta-1,3-galactosyltransferase 7 Short name: Beta-1,3-GalTase 7 Short name: Beta3Gal-T7 Short name: Beta3GalT7 Short name: b3Gal-T7 Beta-3-Gx-T7 UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Short name: BGnT-2 Short name: Beta-1,3-Gn-T2 Short name: Beta-1,3-N-acetylglucosaminyltransferase 2 Short name: Beta3Gn-T2 UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7 |
Gene namesi | Name:B3GNT2 Synonyms:B3GALT7, B3GNT1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:15629, B3GNT2 |
MIMi | 605581, gene |
neXtProti | NX_Q9NY97 |
VEuPathDBi | HostDB:ENSG00000170340.10 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane Curated; Single-pass type II membrane protein Curated
Golgi apparatus
- Golgi apparatus Source: GO_Central
- Golgi membrane Source: Reactome
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 7 | CytoplasmicSequence analysis | 7 | |
Transmembranei | 8 – 28 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 29 – 397 | LumenalSequence analysisAdd BLAST | 369 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 245 | D → A: Loss of enzymatic activity, no loss of B3GNT8-binding. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 10678 |
OpenTargetsi | ENSG00000170340 |
PharmGKBi | PA25218 |
Miscellaneous databases
Pharosi | Q9NY97, Tbio |
Genetic variation databases
BioMutai | B3GNT2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000219170 | 1 – 397 | N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2Add BLAST | 397 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 79 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 89 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 127 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 173 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 219 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
CPTACi | CPTAC-2208 |
EPDi | Q9NY97 |
jPOSTi | Q9NY97 |
MassIVEi | Q9NY97 |
MaxQBi | Q9NY97 |
PaxDbi | Q9NY97 |
PeptideAtlasi | Q9NY97 |
PRIDEi | Q9NY97 |
ProteomicsDBi | 83198 [Q9NY97-1] 83199 [Q9NY97-2] |
PTM databases
GlyConnecti | 1533, 9 N-Linked glycans (6 sites) |
GlyGeni | Q9NY97, 8 sites |
iPTMneti | Q9NY97 |
PhosphoSitePlusi | Q9NY97 |
Expressioni
Tissue specificityi
Ubiquitous.1 Publication
Gene expression databases
Bgeei | ENSG00000170340, Expressed in secondary oocyte and 223 other tissues |
Genevisiblei | Q9NY97, HS |
Organism-specific databases
HPAi | ENSG00000170340, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with B3GNT8; this interaction greatly increases B3GNT2 catalytic activity, independently of B3GNT8 enzymatic activity.
1 PublicationBinary interactionsi
Q9NY97
With | #Exp. | IntAct |
---|---|---|
B3GNT8 [Q7Z7M8] | 2 | EBI-3922389,EBI-20593091 |
Protein-protein interaction databases
BioGRIDi | 115919, 71 interactors |
IntActi | Q9NY97, 24 interactors |
STRINGi | 9606.ENSP00000305595 |
Miscellaneous databases
RNActi | Q9NY97, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyltransferase 31 family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2287, Eukaryota |
GeneTreei | ENSGT00940000155345 |
HOGENOMi | CLU_036849_5_0_1 |
InParanoidi | Q9NY97 |
OMAi | NNICSYT |
OrthoDBi | 1037602at2759 |
PhylomeDBi | Q9NY97 |
TreeFami | TF318639 |
Family and domain databases
InterProi | View protein in InterPro IPR002659, Glyco_trans_31 |
PANTHERi | PTHR11214, PTHR11214, 1 hit |
Pfami | View protein in Pfam PF01762, Galactosyl_T, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q9NY97-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSVGRRRIKL LGILMMANVF IYFIMEVSKS SSQEKNGKGE VIIPKEKFWK
60 70 80 90 100
ISTPPEAYWN REQEKLNRQY NPILSMLTNQ TGEAGRLSNI SHLNYCEPDL
110 120 130 140 150
RVTSVVTGFN NLPDRFKDFL LYLRCRNYSL LIDQPDKCAK KPFLLLAIKS
160 170 180 190 200
LTPHFARRQA IRESWGQESN AGNQTVVRVF LLGQTPPEDN HPDLSDMLKF
210 220 230 240 250
ESEKHQDILM WNYRDTFFNL SLKEVLFLRW VSTSCPDTEF VFKGDDDVFV
260 270 280 290 300
NTHHILNYLN SLSKTKAKDL FIGDVIHNAG PHRDKKLKYY IPEVVYSGLY
310 320 330 340 350
PPYAGGGGFL YSGHLALRLY HITDQVHLYP IDDVYTGMCL QKLGLVPEKH
360 370 380 390
KGFRTFDIEE KNKNNICSYV DLMLVHSRKP QEMIDIWSQL QSAHLKC
Isoform 2 (identifier: Q9NY97-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
1-11: MSVGRRRIKLL → MVSRSLV
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.Curated
Show »Sequence cautioni
The sequence BAA92031 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 11 | L → LL in CAB91546 (Ref. 3) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001791 | 1 – 11 | MSVGRRRIKLL → MVSRSLV in isoform 2. 1 PublicationAdd BLAST | 11 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF092051 mRNA Translation: AAD09764.2 AJ006077 mRNA Translation: CAB91546.1 AF288208 mRNA Translation: AAF97253.1 AF288209 mRNA Translation: AAF97254.1 AB049584 mRNA Translation: BAB21530.1 BC030579 mRNA Translation: AAH30579.1 AC093401 Genomic DNA Translation: AAX93271.1 CH471053 Genomic DNA Translation: EAW99977.1 CH471053 Genomic DNA Translation: EAW99978.1 CH471053 Genomic DNA Translation: EAW99979.1 BC047933 mRNA Translation: AAH47933.1 AK002009 mRNA Translation: BAA92031.1 Different initiation. |
CCDSi | CCDS1870.1 [Q9NY97-1] |
RefSeqi | NP_001306004.1, NM_001319075.1 [Q9NY97-1] NP_006568.2, NM_006577.5 [Q9NY97-1] |
Genome annotation databases
Ensembli | ENST00000301998; ENSP00000305595; ENSG00000170340 [Q9NY97-1] ENST00000405767; ENSP00000384692; ENSG00000170340 [Q9NY97-1] |
GeneIDi | 10678 |
KEGGi | hsa:10678 |
UCSCi | uc002sbs.4, human [Q9NY97-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - GTase UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF092051 mRNA Translation: AAD09764.2 AJ006077 mRNA Translation: CAB91546.1 AF288208 mRNA Translation: AAF97253.1 AF288209 mRNA Translation: AAF97254.1 AB049584 mRNA Translation: BAB21530.1 BC030579 mRNA Translation: AAH30579.1 AC093401 Genomic DNA Translation: AAX93271.1 CH471053 Genomic DNA Translation: EAW99977.1 CH471053 Genomic DNA Translation: EAW99978.1 CH471053 Genomic DNA Translation: EAW99979.1 BC047933 mRNA Translation: AAH47933.1 AK002009 mRNA Translation: BAA92031.1 Different initiation. |
CCDSi | CCDS1870.1 [Q9NY97-1] |
RefSeqi | NP_001306004.1, NM_001319075.1 [Q9NY97-1] NP_006568.2, NM_006577.5 [Q9NY97-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6WMM | X-ray | 1.55 | A/B | 35-397 | [»] | |
6WMN | X-ray | 2.04 | A/B/C/D | 35-397 | [»] | |
6WMO | X-ray | 1.85 | A/B | 35-397 | [»] | |
7JHK | X-ray | 2.34 | A/B/C/D | 45-397 | [»] | |
7JHL | X-ray | 2.26 | A/B | 45-397 | [»] | |
7JHM | X-ray | 2.19 | A/B | 45-397 | [»] | |
7JHN | X-ray | 2.20 | A | 45-397 | [»] | |
7JHO | X-ray | 1.85 | A/B | 45-397 | [»] | |
SMRi | Q9NY97 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115919, 71 interactors |
IntActi | Q9NY97, 24 interactors |
STRINGi | 9606.ENSP00000305595 |
Protein family/group databases
CAZyi | GT31, Glycosyltransferase Family 31 |
PTM databases
GlyConnecti | 1533, 9 N-Linked glycans (6 sites) |
GlyGeni | Q9NY97, 8 sites |
iPTMneti | Q9NY97 |
PhosphoSitePlusi | Q9NY97 |
Genetic variation databases
BioMutai | B3GNT2 |
Proteomic databases
CPTACi | CPTAC-2208 |
EPDi | Q9NY97 |
jPOSTi | Q9NY97 |
MassIVEi | Q9NY97 |
MaxQBi | Q9NY97 |
PaxDbi | Q9NY97 |
PeptideAtlasi | Q9NY97 |
PRIDEi | Q9NY97 |
ProteomicsDBi | 83198 [Q9NY97-1] 83199 [Q9NY97-2] |
Protocols and materials databases
Antibodypediai | 1107, 177 antibodies |
DNASUi | 10678 |
Genome annotation databases
Ensembli | ENST00000301998; ENSP00000305595; ENSG00000170340 [Q9NY97-1] ENST00000405767; ENSP00000384692; ENSG00000170340 [Q9NY97-1] |
GeneIDi | 10678 |
KEGGi | hsa:10678 |
UCSCi | uc002sbs.4, human [Q9NY97-1] |
Organism-specific databases
CTDi | 10678 |
DisGeNETi | 10678 |
GeneCardsi | B3GNT2 |
HGNCi | HGNC:15629, B3GNT2 |
HPAi | ENSG00000170340, Low tissue specificity |
MIMi | 605581, gene |
neXtProti | NX_Q9NY97 |
OpenTargetsi | ENSG00000170340 |
PharmGKBi | PA25218 |
VEuPathDBi | HostDB:ENSG00000170340.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2287, Eukaryota |
GeneTreei | ENSGT00940000155345 |
HOGENOMi | CLU_036849_5_0_1 |
InParanoidi | Q9NY97 |
OMAi | NNICSYT |
OrthoDBi | 1037602at2759 |
PhylomeDBi | Q9NY97 |
TreeFami | TF318639 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BioCyci | MetaCyc:ENSG00000170340-MONOMER |
PathwayCommonsi | Q9NY97 |
Reactomei | R-HSA-2022854, Keratan sulfate biosynthesis R-HSA-913709, O-linked glycosylation of mucins |
Miscellaneous databases
BioGRID-ORCSi | 10678, 30 hits in 995 CRISPR screens |
ChiTaRSi | B3GNT2, human |
GeneWikii | B3GNT2 |
GenomeRNAii | 10678 |
Pharosi | Q9NY97, Tbio |
PROi | PR:Q9NY97 |
RNActi | Q9NY97, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000170340, Expressed in secondary oocyte and 223 other tissues |
Genevisiblei | Q9NY97, HS |
Family and domain databases
InterProi | View protein in InterPro IPR002659, Glyco_trans_31 |
PANTHERi | PTHR11214, PTHR11214, 1 hit |
Pfami | View protein in Pfam PF01762, Galactosyl_T, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | B3GN2_HUMAN | |
Accessioni | Q9NY97Primary (citable) accession number: Q9NY97 Secondary accession number(s): Q54AC1 Q9NUT9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | April 11, 2003 | |
Last modified: | April 7, 2021 | |
This is version 172 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families