UniProtKB - Q9NY33 (DPP3_HUMAN)
Protein
Dipeptidyl peptidase 3
Gene
DPP3
Organism
Homo sapiens (Human)
Status
Functioni
Cleaves and degrades bioactive peptides, including angiotensin, Leu-enkephalin and Met-enkephalin (PubMed:3233187, PubMed:1515063). Also cleaves Arg-Arg-beta-naphthylamide (in vitro) (PubMed:9425109, PubMed:3233187, PubMed:11209758).4 Publications
Catalytic activityi
- Release of an N-terminal dipeptide from a peptide comprising four or more residues, with broad specificity. Also acts on dipeptidyl 2-naphthylamides.4 Publications EC:3.4.14.4
Cofactori
Zn2+3 PublicationsNote: Binds 1 zinc ion per subunit.1 Publication
Activity regulationi
Activated by Co2+. Inhibited by EDTA and o-phenanthroline (in vitro).4 Publications
pH dependencei
Optimum pH is 8.8.3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 450 | Zinc; catalyticCombined sources1 Publication | 1 | |
Active sitei | 451 | By similarity | 1 | |
Metal bindingi | 455 | Zinc; catalyticCombined sources1 Publication | 1 | |
Metal bindingi | 508 | Zinc; catalyticCombined sources1 Publication | 1 |
GO - Molecular functioni
- dipeptidyl-peptidase activity Source: UniProtKB
- metalloaminopeptidase activity Source: InterPro
- zinc ion binding Source: UniProtKB
GO - Biological processi
- proteolysis Source: UniProtKB
Keywordsi
Molecular function | Aminopeptidase, Hydrolase, Metalloprotease, Protease |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.4.14.4, 2681 |
PathwayCommonsi | Q9NY33 |
Protein family/group databases
MEROPSi | M49.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Dipeptidyl peptidase 3 (EC:3.4.14.44 Publications)Alternative name(s): Dipeptidyl aminopeptidase III Dipeptidyl arylamidase III Dipeptidyl peptidase III Short name: DPP III Enkephalinase B |
Gene namesi | Name:DPP3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000254986.7 |
HGNCi | HGNC:3008, DPP3 |
MIMi | 606818, gene |
neXtProti | NX_Q9NY33 |
Subcellular locationi
Cytosol
- cytosol 2 Publications
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 10072 |
OpenTargetsi | ENSG00000254986 |
PharmGKBi | PA27466 |
Miscellaneous databases
Pharosi | Q9NY33, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4520 |
Polymorphism and mutation databases
BioMutai | DPP3 |
DMDMi | 20532389 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000078238 | 2 – 737 | Dipeptidyl peptidase 3Add BLAST | 736 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
CPTACi | CPTAC-58 CPTAC-59 |
EPDi | Q9NY33 |
jPOSTi | Q9NY33 |
MassIVEi | Q9NY33 |
MaxQBi | Q9NY33 |
PaxDbi | Q9NY33 |
PeptideAtlasi | Q9NY33 |
PRIDEi | Q9NY33 |
ProteomicsDBi | 27822 83159 [Q9NY33-1] 83160 [Q9NY33-2] |
PTM databases
iPTMneti | Q9NY33 |
MetOSitei | Q9NY33 |
PhosphoSitePlusi | Q9NY33 |
SwissPalmi | Q9NY33 |
Expressioni
Tissue specificityi
Detected in placenta (at protein level) (PubMed:3233187). Detected in erythrocytes (at protein level) (PubMed:1515063).2 Publications
Gene expression databases
Bgeei | ENSG00000254986, Expressed in mucosa of transverse colon and 208 other tissues |
ExpressionAtlasi | Q9NY33, baseline and differential |
Genevisiblei | Q9NY33, HS |
Organism-specific databases
HPAi | ENSG00000254986, Low tissue specificity |
Interactioni
Binary interactionsi
Q9NY33
With | #Exp. | IntAct |
---|---|---|
KEAP1 [Q14145] | 8 | EBI-718333,EBI-751001 |
Protein-protein interaction databases
BioGRIDi | 115383, 27 interactors |
DIPi | DIP-53793N |
IntActi | Q9NY33, 9 interactors |
MINTi | Q9NY33 |
STRINGi | 9606.ENSP00000353701 |
Chemistry databases
BindingDBi | Q9NY33 |
Miscellaneous databases
RNActi | Q9NY33, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9NY33 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9NY33 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase M49 family.Curated
Phylogenomic databases
eggNOGi | KOG3675, Eukaryota |
GeneTreei | ENSGT00390000007335 |
HOGENOMi | CLU_011977_0_0_1 |
InParanoidi | Q9NY33 |
OrthoDBi | 1448344at2759 |
PhylomeDBi | Q9NY33 |
TreeFami | TF300598 |
Family and domain databases
InterProi | View protein in InterPro IPR005317, Dipeptidyl-peptase3 IPR039461, Peptidase_M49 |
PANTHERi | PTHR23422, PTHR23422, 1 hit |
Pfami | View protein in Pfam PF03571, Peptidase_M49, 1 hit |
PIRSFi | PIRSF007828, Dipeptidyl-peptidase_III, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9NY33-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MADTQYILPN DIGVSSLDCR EAFRLLSPTE RLYAYHLSRA AWYGGLAVLL
60 70 80 90 100
QTSPEAPYIY ALLSRLFRAQ DPDQLRQHAL AEGLTEEEYQ AFLVYAAGVY
110 120 130 140 150
SNMGNYKSFG DTKFVPNLPK EKLERVILGS EAAQQHPEEV RGLWQTCGEL
160 170 180 190 200
MFSLEPRLRH LGLGKEGITT YFSGNCTMED AKLAQDFLDS QNLSAYNTRL
210 220 230 240 250
FKEVDGEGKP YYEVRLASVL GSEPSLDSEV TSKLKSYEFR GSPFQVTRGD
260 270 280 290 300
YAPILQKVVE QLEKAKAYAA NSHQGQMLAQ YIESFTQGSI EAHKRGSRFW
310 320 330 340 350
IQDKGPIVES YIGFIESYRD PFGSRGEFEG FVAVVNKAMS AKFERLVASA
360 370 380 390 400
EQLLKELPWP PTFEKDKFLT PDFTSLDVLT FAGSGIPAGI NIPNYDDLRQ
410 420 430 440 450
TEGFKNVSLG NVLAVAYATQ REKLTFLEED DKDLYILWKG PSFDVQVGLH
460 470 480 490 500
ELLGHGSGKL FVQDEKGAFN FDQETVINPE TGEQIQSWYR SGETWDSKFS
510 520 530 540 550
TIASSYEECR AESVGLYLCL HPQVLEIFGF EGADAEDVIY VNWLNMVRAG
560 570 580 590 600
LLALEFYTPE AFNWRQAHMQ ARFVILRVLL EAGEGLVTIT PTTGSDGRPD
610 620 630 640 650
ARVRLDRSKI RSVGKPALER FLRRLQVLKS TGDVAGGRAL YEGYATVTDA
660 670 680 690 700
PPECFLTLRD TVLLRKESRK LIVQPNTRLE GSDVQLLEYE ASAAGLIRSF
710 720 730
SERFPEDGPE LEEILTQLAT ADARFWKGPS EAPSGQA
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V180 | G3V180_HUMAN | Dipeptidyl peptidase 3 | DPP3 hCG_2016942 | 757 | Annotation score: | ||
G3V1D3 | G3V1D3_HUMAN | Dipeptidyl peptidase 3 | DPP3 hCG_2016942 | 756 | Annotation score: | ||
E9PNX5 | E9PNX5_HUMAN | Dipeptidyl peptidase 3 | DPP3 | 104 | Annotation score: | ||
E9PPK9 | E9PPK9_HUMAN | Dipeptidyl peptidase 3 | DPP3 | 201 | Annotation score: | ||
E9PQ14 | E9PQ14_HUMAN | Dipeptidyl peptidase 3 | DPP3 | 177 | Annotation score: | ||
E9PKK8 | E9PKK8_HUMAN | Dipeptidyl peptidase 3 | DPP3 | 170 | Annotation score: | ||
E9PQF2 | E9PQF2_HUMAN | Dipeptidyl peptidase 3 | DPP3 | 90 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 224 | P → S in BAG59686 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 417 – 419 | YAT → TA in BAA75785 (PubMed:9425109).Curated | 3 | |
Sequence conflicti | 697 | I → Y in BAA75785 (PubMed:9425109).Curated | 1 |
Mass spectrometryi
Molecular mass is 82500±60 Da. Determined by MALDI. 1 Publication
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_033494 | 76 | R → H1 PublicationCorresponds to variant dbSNP:rs11826683EnsemblClinVar. | 1 | |
Natural variantiVAR_033495 | 145 | Q → H1 PublicationCorresponds to variant dbSNP:rs11550299Ensembl. | 1 | |
Natural variantiVAR_021850 | 678 | R → H2 PublicationsCorresponds to variant dbSNP:rs2305535Ensembl. | 1 | |
Natural variantiVAR_051597 | 690 | E → K. Corresponds to variant dbSNP:rs12421620Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_044696 | 91 – 120 | Missing in isoform 4. 1 PublicationAdd BLAST | 30 | |
Alternative sequenceiVSP_005510 | 182 – 601 | Missing in isoform 2. 1 PublicationAdd BLAST | 420 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ271216 mRNA Translation: CAB72433.1 AK021449 mRNA Translation: BAB13828.1 AK297199 mRNA Translation: BAG59686.1 AK315478 mRNA Translation: BAG37862.1 AP002748 Genomic DNA No translation available. BC001446 mRNA Translation: AAH01446.1 BC007221 mRNA Translation: AAH07221.1 BC014038 mRNA Translation: AAH14038.1 BC024271 mRNA Translation: AAH24271.1 AB017970 mRNA Translation: BAA75785.1 |
CCDSi | CCDS58147.1 [Q9NY33-4] CCDS8141.1 [Q9NY33-1] |
RefSeqi | NP_001243599.1, NM_001256670.1 [Q9NY33-4] NP_005691.2, NM_005700.4 [Q9NY33-1] NP_569710.2, NM_130443.3 [Q9NY33-1] |
Genome annotation databases
Ensembli | ENST00000530165; ENSP00000436941; ENSG00000254986 [Q9NY33-4] ENST00000541961; ENSP00000440502; ENSG00000254986 [Q9NY33-1] |
GeneIDi | 10072 |
KEGGi | hsa:10072 |
UCSCi | uc010rpe.3, human [Q9NY33-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ271216 mRNA Translation: CAB72433.1 AK021449 mRNA Translation: BAB13828.1 AK297199 mRNA Translation: BAG59686.1 AK315478 mRNA Translation: BAG37862.1 AP002748 Genomic DNA No translation available. BC001446 mRNA Translation: AAH01446.1 BC007221 mRNA Translation: AAH07221.1 BC014038 mRNA Translation: AAH14038.1 BC024271 mRNA Translation: AAH24271.1 AB017970 mRNA Translation: BAA75785.1 |
CCDSi | CCDS58147.1 [Q9NY33-4] CCDS8141.1 [Q9NY33-1] |
RefSeqi | NP_001243599.1, NM_001256670.1 [Q9NY33-4] NP_005691.2, NM_005700.4 [Q9NY33-1] NP_569710.2, NM_130443.3 [Q9NY33-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3FVY | X-ray | 1.90 | A | 1-726 | [»] | |
3T6B | X-ray | 2.40 | A/B | 1-726 | [»] | |
3T6J | X-ray | 2.98 | A | 1-726 | [»] | |
5E2Q | X-ray | 2.40 | A | 1-726 | [»] | |
5E33 | X-ray | 1.84 | A | 1-726 | [»] | |
5E3A | X-ray | 2.05 | A | 1-726 | [»] | |
5E3C | X-ray | 2.77 | A | 1-726 | [»] | |
5EGY | X-ray | 2.74 | A | 1-726 | [»] | |
5EHH | X-ray | 2.38 | A | 1-726 | [»] | |
SMRi | Q9NY33 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115383, 27 interactors |
DIPi | DIP-53793N |
IntActi | Q9NY33, 9 interactors |
MINTi | Q9NY33 |
STRINGi | 9606.ENSP00000353701 |
Chemistry databases
BindingDBi | Q9NY33 |
ChEMBLi | CHEMBL4520 |
Protein family/group databases
MEROPSi | M49.001 |
PTM databases
iPTMneti | Q9NY33 |
MetOSitei | Q9NY33 |
PhosphoSitePlusi | Q9NY33 |
SwissPalmi | Q9NY33 |
Polymorphism and mutation databases
BioMutai | DPP3 |
DMDMi | 20532389 |
Proteomic databases
CPTACi | CPTAC-58 CPTAC-59 |
EPDi | Q9NY33 |
jPOSTi | Q9NY33 |
MassIVEi | Q9NY33 |
MaxQBi | Q9NY33 |
PaxDbi | Q9NY33 |
PeptideAtlasi | Q9NY33 |
PRIDEi | Q9NY33 |
ProteomicsDBi | 27822 83159 [Q9NY33-1] 83160 [Q9NY33-2] |
Protocols and materials databases
Antibodypediai | 52373, 389 antibodies |
DNASUi | 10072 |
Genome annotation databases
Ensembli | ENST00000530165; ENSP00000436941; ENSG00000254986 [Q9NY33-4] ENST00000541961; ENSP00000440502; ENSG00000254986 [Q9NY33-1] |
GeneIDi | 10072 |
KEGGi | hsa:10072 |
UCSCi | uc010rpe.3, human [Q9NY33-1] |
Organism-specific databases
CTDi | 10072 |
DisGeNETi | 10072 |
EuPathDBi | HostDB:ENSG00000254986.7 |
GeneCardsi | DPP3 |
HGNCi | HGNC:3008, DPP3 |
HPAi | ENSG00000254986, Low tissue specificity |
MIMi | 606818, gene |
neXtProti | NX_Q9NY33 |
OpenTargetsi | ENSG00000254986 |
PharmGKBi | PA27466 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3675, Eukaryota |
GeneTreei | ENSGT00390000007335 |
HOGENOMi | CLU_011977_0_0_1 |
InParanoidi | Q9NY33 |
OrthoDBi | 1448344at2759 |
PhylomeDBi | Q9NY33 |
TreeFami | TF300598 |
Enzyme and pathway databases
BRENDAi | 3.4.14.4, 2681 |
PathwayCommonsi | Q9NY33 |
Miscellaneous databases
BioGRID-ORCSi | 10072, 22 hits in 845 CRISPR screens |
ChiTaRSi | DPP3, human |
EvolutionaryTracei | Q9NY33 |
GeneWikii | DPP3 |
GenomeRNAii | 10072 |
Pharosi | Q9NY33, Tbio |
RNActi | Q9NY33, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000254986, Expressed in mucosa of transverse colon and 208 other tissues |
ExpressionAtlasi | Q9NY33, baseline and differential |
Genevisiblei | Q9NY33, HS |
Family and domain databases
InterProi | View protein in InterPro IPR005317, Dipeptidyl-peptase3 IPR039461, Peptidase_M49 |
PANTHERi | PTHR23422, PTHR23422, 1 hit |
Pfami | View protein in Pfam PF03571, Peptidase_M49, 1 hit |
PIRSFi | PIRSF007828, Dipeptidyl-peptidase_III, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPP3_HUMAN | |
Accessioni | Q9NY33Primary (citable) accession number: Q9NY33 Secondary accession number(s): B2RDB5 Q9HAL6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | May 10, 2002 | |
Last modified: | December 2, 2020 | |
This is version 186 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Peptidase families
Classification of peptidase families and list of entries