UniProtKB - Q9NY25 (CLC5A_HUMAN)
C-type lectin domain family 5 member A
CLEC5A
Functioni
Functions as a positive regulator of osteoclastogenesis (By similarity).
Cell surface receptor that signals via TYROBP (PubMed:10449773).
Regulates inflammatory responses (By similarity).
By similarity1 Publication(Microbial infection) Critical macrophage receptor for dengue virus serotypes 1-4 (PubMed:18496526, PubMed:21566123).
The binding of dengue virus to CLEC5A triggers signaling through the phosphorylation of TYROBP (PubMed:18496526).
This interaction does not result in viral entry, but stimulates pro-inflammatory cytokine release (PubMed:18496526).
2 PublicationsMiscellaneous
GO - Molecular functioni
- carbohydrate binding Source: UniProtKB-KW
- virus receptor activity Source: UniProtKB
GO - Biological processi
- cellular defense response Source: ProtInc
- innate immune response Source: UniProtKB
- myeloid cell differentiation Source: Ensembl
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of myeloid cell apoptotic process Source: Ensembl
- osteoblast development Source: UniProtKB
- positive regulation of cytokine production Source: Ensembl
- signal transduction Source: ProtInc
Keywordsi
Molecular function | Host cell receptor for virus entry, Receptor |
Biological process | Host-virus interaction, Immunity, Innate immunity |
Ligand | Lectin |
Enzyme and pathway databases
PathwayCommonsi | Q9NY25 |
Reactomei | R-HSA-2172127, DAP12 interactions R-HSA-6798695, Neutrophil degranulation |
SignaLinki | Q9NY25 |
Protein family/group databases
UniLectini | Q9NY25 |
Names & Taxonomyi
Protein namesi | Recommended name: C-type lectin domain family 5 member AAlternative name(s): C-type lectin superfamily member 5 Myeloid DAP12-associating lectin 1 Short name: MDL-1 |
Gene namesi | Name:CLEC5A Synonyms:CLECSF5, MDL1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:2054, CLEC5A |
MIMi | 604987, gene |
neXtProti | NX_Q9NY25 |
VEuPathDBi | HostDB:ENSG00000258227 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Single-pass type II membrane protein 1 Publication
Cytosol
- cytosol Source: HPA
Plasma Membrane
- integral component of plasma membrane Source: ProtInc
- plasma membrane Source: HPA
Other locations
- cell surface Source: UniProtKB
- specific granule membrane Source: Reactome
- tertiary granule membrane Source: Reactome
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 4 | CytoplasmicSequence analysis | 4 | |
Transmembranei | 5 – 27 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 23 | |
Topological domaini | 28 – 188 | ExtracellularSequence analysisAdd BLAST | 161 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 16 | K → I: Abolishes interaction with TYROBP. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 23601 |
OpenTargetsi | ENSG00000258227 |
PharmGKBi | PA26583 |
Miscellaneous databases
Pharosi | Q9NY25, Tbio |
Genetic variation databases
BioMutai | CLEC5A |
DMDMi | 59797971 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046632 | 1 – 188 | C-type lectin domain family 5 member AAdd BLAST | 188 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 32 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 71 ↔ 82 | PROSITE-ProRule annotation | ||
Glycosylationi | 93 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 99 ↔ 183 | PROSITE-ProRule annotation | ||
Glycosylationi | 144 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 151 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 161 ↔ 175 | PROSITE-ProRule annotation |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | Q9NY25 |
jPOSTi | Q9NY25 |
MassIVEi | Q9NY25 |
PaxDbi | Q9NY25 |
PeptideAtlasi | Q9NY25 |
PRIDEi | Q9NY25 |
ProteomicsDBi | 83151 [Q9NY25-1] 83152 [Q9NY25-2] |
PTM databases
GlyGeni | Q9NY25, 4 sites |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000258227, Expressed in bone marrow and 120 other tissues |
ExpressionAtlasi | Q9NY25, baseline and differential |
Genevisiblei | Q9NY25, HS |
Organism-specific databases
HPAi | ENSG00000258227, Tissue enriched (bone) |
Interactioni
Subunit structurei
Monomer (PubMed:21566123). Homodimer (PubMed:21566123). The majority of CLEC5A is expressed as a monomeric form on macrophages (PubMed:21566123).
Interacts with TYROBP/DAP12 (PubMed:10449773). The interaction with TYROBP is required for CLEC5A cell surface expression (PubMed:10449773).
Interacts with HCST/DAP10 (By similarity).
Forms a CLEC5A/TYROBP/HCST trimolecular complex depending almost solely on TYROBP (By similarity).
By similarity2 PublicationsProtein-protein interaction databases
BioGRIDi | 117135, 30 interactors |
DIPi | DIP-60627N |
IntActi | Q9NY25, 15 interactors |
STRINGi | 9606.ENSP00000449999 |
Miscellaneous databases
RNActi | Q9NY25, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9NY25 |
SMRi | Q9NY25 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9NY25 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 78 – 184 | C-type lectinPROSITE-ProRule annotationAdd BLAST | 107 |
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4297, Eukaryota |
GeneTreei | ENSGT00910000144330 |
InParanoidi | Q9NY25 |
OMAi | WHWIDNS |
PhylomeDBi | Q9NY25 |
TreeFami | TF337735 |
Family and domain databases
CDDi | cd03593, CLECT_NK_receptors_like, 1 hit |
Gene3Di | 3.10.100.10, 1 hit |
InterProi | View protein in InterPro IPR001304, C-type_lectin-like IPR016186, C-type_lectin-like/link_sf IPR016187, CTDL_fold IPR033992, NKR-like_CTLD |
Pfami | View protein in Pfam PF00059, Lectin_C, 1 hit |
SMARTi | View protein in SMART SM00034, CLECT, 1 hit |
SUPFAMi | SSF56436, SSF56436, 1 hit |
PROSITEi | View protein in PROSITE PS50041, C_TYPE_LECTIN_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNWHMIISGL IVVVLKVVGM TLFLLYFPQI FNKSNDGFTT TRSYGTVSQI
60 70 80 90 100
FGSSSPSPNG FITTRSYGTV CPKDWEFYQA RCFFLSTSES SWNESRDFCK
110 120 130 140 150
GKGSTLAIVN TPEKLKFLQD ITDAEKYFIG LIYHREEKRW RWINNSVFNG
160 170 180
NVTNQNQNFN CATIGLTKTF DAASCDISYR RICEKNAK
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ14DL9 | Q14DL9_HUMAN | C-type lectin domain family 5 membe... | CLEC5A | 165 | Annotation score: | ||
C9JPR7 | C9JPR7_HUMAN | C-type lectin domain family 5 membe... | CLEC5A | 155 | Annotation score: | ||
F8WCL0 | F8WCL0_HUMAN | C-type lectin domain family 5 membe... | CLEC5A | 53 | Annotation score: | ||
C9JCE4 | C9JCE4_HUMAN | C-type lectin domain family 5 membe... | CLEC5A | 84 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_050110 | 141 | R → H. Corresponds to variant dbSNP:rs35942193Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_012839 | 116 | Missing in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF139768 mRNA Translation: AAF02491.1 AJ271684 mRNA Translation: CAB71334.1 AC073647 Genomic DNA Translation: AAS07444.1 BC093714 mRNA Translation: AAH93714.1 BC112099 mRNA Translation: AAI12100.1 BC113098 mRNA Translation: AAI13099.1 |
CCDSi | CCDS5870.1 [Q9NY25-1] |
RefSeqi | NP_037384.1, NM_013252.2 [Q9NY25-1] |
Genome annotation databases
Ensembli | ENST00000546910.6; ENSP00000449999.1; ENSG00000258227.7 |
GeneIDi | 23601 |
KEGGi | hsa:23601 |
MANE-Selecti | ENST00000546910.6; ENSP00000449999.1; NM_013252.3; NP_037384.1 |
UCSCi | uc003vwv.1, human [Q9NY25-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - Glycan Binding MDL-1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF139768 mRNA Translation: AAF02491.1 AJ271684 mRNA Translation: CAB71334.1 AC073647 Genomic DNA Translation: AAS07444.1 BC093714 mRNA Translation: AAH93714.1 BC112099 mRNA Translation: AAI12100.1 BC113098 mRNA Translation: AAI13099.1 |
CCDSi | CCDS5870.1 [Q9NY25-1] |
RefSeqi | NP_037384.1, NM_013252.2 [Q9NY25-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2YHF | X-ray | 1.90 | A/B/C/D/E/F/G/H/I | 70-187 | [»] | |
AlphaFoldDBi | Q9NY25 | |||||
SMRi | Q9NY25 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 117135, 30 interactors |
DIPi | DIP-60627N |
IntActi | Q9NY25, 15 interactors |
STRINGi | 9606.ENSP00000449999 |
Protein family/group databases
UniLectini | Q9NY25 |
PTM databases
GlyGeni | Q9NY25, 4 sites |
Genetic variation databases
BioMutai | CLEC5A |
DMDMi | 59797971 |
Proteomic databases
EPDi | Q9NY25 |
jPOSTi | Q9NY25 |
MassIVEi | Q9NY25 |
PaxDbi | Q9NY25 |
PeptideAtlasi | Q9NY25 |
PRIDEi | Q9NY25 |
ProteomicsDBi | 83151 [Q9NY25-1] 83152 [Q9NY25-2] |
Protocols and materials databases
Antibodypediai | 50228, 263 antibodies from 26 providers |
DNASUi | 23601 |
Genome annotation databases
Ensembli | ENST00000546910.6; ENSP00000449999.1; ENSG00000258227.7 |
GeneIDi | 23601 |
KEGGi | hsa:23601 |
MANE-Selecti | ENST00000546910.6; ENSP00000449999.1; NM_013252.3; NP_037384.1 |
UCSCi | uc003vwv.1, human [Q9NY25-1] |
Organism-specific databases
CTDi | 23601 |
DisGeNETi | 23601 |
GeneCardsi | CLEC5A |
HGNCi | HGNC:2054, CLEC5A |
HPAi | ENSG00000258227, Tissue enriched (bone) |
MIMi | 604987, gene |
neXtProti | NX_Q9NY25 |
OpenTargetsi | ENSG00000258227 |
PharmGKBi | PA26583 |
VEuPathDBi | HostDB:ENSG00000258227 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4297, Eukaryota |
GeneTreei | ENSGT00910000144330 |
InParanoidi | Q9NY25 |
OMAi | WHWIDNS |
PhylomeDBi | Q9NY25 |
TreeFami | TF337735 |
Enzyme and pathway databases
PathwayCommonsi | Q9NY25 |
Reactomei | R-HSA-2172127, DAP12 interactions R-HSA-6798695, Neutrophil degranulation |
SignaLinki | Q9NY25 |
Miscellaneous databases
BioGRID-ORCSi | 23601, 8 hits in 1063 CRISPR screens |
ChiTaRSi | CLEC5A, human |
EvolutionaryTracei | Q9NY25 |
GeneWikii | CLEC5A |
GenomeRNAii | 23601 |
Pharosi | Q9NY25, Tbio |
PROi | PR:Q9NY25 |
RNActi | Q9NY25, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000258227, Expressed in bone marrow and 120 other tissues |
ExpressionAtlasi | Q9NY25, baseline and differential |
Genevisiblei | Q9NY25, HS |
Family and domain databases
CDDi | cd03593, CLECT_NK_receptors_like, 1 hit |
Gene3Di | 3.10.100.10, 1 hit |
InterProi | View protein in InterPro IPR001304, C-type_lectin-like IPR016186, C-type_lectin-like/link_sf IPR016187, CTDL_fold IPR033992, NKR-like_CTLD |
Pfami | View protein in Pfam PF00059, Lectin_C, 1 hit |
SMARTi | View protein in SMART SM00034, CLECT, 1 hit |
SUPFAMi | SSF56436, SSF56436, 1 hit |
PROSITEi | View protein in PROSITE PS50041, C_TYPE_LECTIN_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CLC5A_HUMAN | |
Accessioni | Q9NY25Primary (citable) accession number: Q9NY25 Secondary accession number(s): Q52M11, Q9UKQ0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 15, 2005 |
Last sequence update: | October 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references