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Protein

E3 ubiquitin-protein ligase RNF186

Gene

RNF186

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin protein ligase that is part of an apoptotic signaling pathway activated by endoplasmic reticulum stress (PubMed:23896122). In that process, stimulates the expression of proteins specific of the unfolded protein response (UPR), ubiquitinates BNIP1 and regulates its localization to the mitochondrion and induces calcium release from the endoplasmic reticulum that ultimately leads to cell apoptosis (PubMed:23896122).1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri40 – 86RING-typePROSITE-ProRule annotationAdd BLAST47

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF186Curated (EC:2.3.2.271 Publication)
Alternative name(s):
RING finger protein 186Imported
Gene namesi
Name:RNF186Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000178828.6
HGNCiHGNC:25978 RNF186
neXtProtiNX_Q9NXI6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Transmembranei180 – 200HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi58 – 66CQHAFCAIC → AQWAFAAIA: Loss of autoubiquitination; loss of BNIP1 polyubiquitination; no effect on interaction with BNIP1; decreased effect on calcium release from the endoplasmic reticulum. 1 Publication9

Organism-specific databases

DisGeNETi54546
OpenTargetsiENSG00000178828
PharmGKBiPA142671057

Polymorphism and mutation databases

BioMutaiRNF186
DMDMi74761796

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002616241 – 227E3 ubiquitin-protein ligase RNF186Add BLAST227

Post-translational modificationi

Polyubiquitinated. 'Lys-29'-linked autoubiquitination leads to proteasomal degradation.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ9NXI6
PeptideAtlasiQ9NXI6
PRIDEiQ9NXI6
ProteomicsDBi83103

PTM databases

iPTMnetiQ9NXI6
PhosphoSitePlusiQ9NXI6

Expressioni

Gene expression databases

BgeeiENSG00000178828 Expressed in 46 organ(s), highest expression level in duodenum
CleanExiHS_RNF186
GenevisibleiQ9NXI6 HS

Organism-specific databases

HPAiHPA034547
HPA035558

Interactioni

Subunit structurei

Interacts with BNIP1.1 Publication

Protein-protein interaction databases

BioGridi120033, 3 interactors
IntActiQ9NXI6, 4 interactors
STRINGi9606.ENSP00000364263

Structurei

3D structure databases

ProteinModelPortaliQ9NXI6
SMRiQ9NXI6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-type domain is required for ubiquitination.1 Publication

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri40 – 86RING-typePROSITE-ProRule annotationAdd BLAST47

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG2177 Eukaryota
ENOG4111G04 LUCA
GeneTreeiENSGT00510000049175
HOGENOMiHOG000231633
HOVERGENiHBG080364
InParanoidiQ9NXI6
OMAiECLVCRE
OrthoDBiEOG091G0KUF
PhylomeDBiQ9NXI6
TreeFamiTF331690

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR038878 RNF186
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR16450 PTHR16450, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9NXI6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MACTKTLQQS QPISAGATTT TTAVAPAGGH SGSTECDLEC LVCREPYSCP
60 70 80 90 100
RLPKLLACQH AFCAICLKLL LCVQDNTWSI TCPLCRKVTA VPGGLICSLR
110 120 130 140 150
DHEAVVGQLA QPCTEVSLCP QGLVDPADLA AGHPSLVGED GQDEVSANHV
160 170 180 190 200
AARRLAAHLL LLALLIILIG PFIYPGVLRW VLTFIIALAL LMSTLFCCLP
210 220
STRGSCWPSS RTLFCREQKH SHISSIA
Length:227
Mass (Da):24,145
Last modified:October 1, 2000 - v1
Checksum:iB856398C3C73DE3A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti209S → P in BAD96711 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02946023A → T. Corresponds to variant dbSNP:rs1541185Ensembl.1
Natural variantiVAR_052111208P → T. Corresponds to variant dbSNP:rs35541730Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000232 mRNA Translation: BAA91024.1
CR457228 mRNA Translation: CAG33509.1
AK222991 mRNA Translation: BAD96711.1
AL391883 Genomic DNA No translation available.
BC030960 mRNA Translation: AAH30960.1
BC051880 mRNA Translation: AAH51880.1
CCDSiCCDS199.1
RefSeqiNP_061935.1, NM_019062.1
UniGeneiHs.124835

Genome annotation databases

EnsembliENST00000375121; ENSP00000364263; ENSG00000178828
GeneIDi54546
KEGGihsa:54546
UCSCiuc001bcr.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000232 mRNA Translation: BAA91024.1
CR457228 mRNA Translation: CAG33509.1
AK222991 mRNA Translation: BAD96711.1
AL391883 Genomic DNA No translation available.
BC030960 mRNA Translation: AAH30960.1
BC051880 mRNA Translation: AAH51880.1
CCDSiCCDS199.1
RefSeqiNP_061935.1, NM_019062.1
UniGeneiHs.124835

3D structure databases

ProteinModelPortaliQ9NXI6
SMRiQ9NXI6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120033, 3 interactors
IntActiQ9NXI6, 4 interactors
STRINGi9606.ENSP00000364263

PTM databases

iPTMnetiQ9NXI6
PhosphoSitePlusiQ9NXI6

Polymorphism and mutation databases

BioMutaiRNF186
DMDMi74761796

Proteomic databases

PaxDbiQ9NXI6
PeptideAtlasiQ9NXI6
PRIDEiQ9NXI6
ProteomicsDBi83103

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000375121; ENSP00000364263; ENSG00000178828
GeneIDi54546
KEGGihsa:54546
UCSCiuc001bcr.3 human

Organism-specific databases

CTDi54546
DisGeNETi54546
EuPathDBiHostDB:ENSG00000178828.6
GeneCardsiRNF186
HGNCiHGNC:25978 RNF186
HPAiHPA034547
HPA035558
neXtProtiNX_Q9NXI6
OpenTargetsiENSG00000178828
PharmGKBiPA142671057
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2177 Eukaryota
ENOG4111G04 LUCA
GeneTreeiENSGT00510000049175
HOGENOMiHOG000231633
HOVERGENiHBG080364
InParanoidiQ9NXI6
OMAiECLVCRE
OrthoDBiEOG091G0KUF
PhylomeDBiQ9NXI6
TreeFamiTF331690

Enzyme and pathway databases

UniPathwayi
UPA00143

Miscellaneous databases

GenomeRNAii54546
PROiPR:Q9NXI6

Gene expression databases

BgeeiENSG00000178828 Expressed in 46 organ(s), highest expression level in duodenum
CleanExiHS_RNF186
GenevisibleiQ9NXI6 HS

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR038878 RNF186
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR16450 PTHR16450, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRN186_HUMAN
AccessioniPrimary (citable) accession number: Q9NXI6
Secondary accession number(s): Q53GE0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 1, 2000
Last modified: October 10, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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