UniProtKB - Q9NXH9 (TRM1_HUMAN)
Protein
tRNA (guanine(26)-N(2))-dimethyltransferase
Gene
TRMT1
Organism
Homo sapiens (Human)
Status
Functioni
Dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.
Catalytic activityi
- guanosine26 in tRNA + 2 S-adenosyl-L-methionine = 2 H+ + N2-dimethylguanosine26 in tRNA + 2 S-adenosyl-L-homocysteinePROSITE-ProRule annotationEC:2.1.1.216PROSITE-ProRule annotation
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 600 – 627 | C3H1-typePROSITE-ProRule annotationAdd BLAST | 28 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- RNA binding Source: UniProtKB
- tRNA (guanine-N2-)-methyltransferase activity Source: Reactome
- tRNA binding Source: UniProtKB-KW
GO - Biological processi
- tRNA modification Source: Reactome
- tRNA N2-guanine methylation Source: GO_Central
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase, tRNA-binding |
Biological process | tRNA processing |
Ligand | Metal-binding, S-adenosyl-L-methionine, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9NXH9 |
Reactomei | R-HSA-6782315, tRNA modification in the nucleus and cytosol |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA (guanine(26)-N(2))-dimethyltransferase (EC:2.1.1.216)Alternative name(s): tRNA 2,2-dimethylguanosine-26 methyltransferase tRNA(guanine-26,N(2)-N(2)) methyltransferase tRNA(m(2,2)G26)dimethyltransferase |
Gene namesi | Name:TRMT1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:25980, TRMT1 |
MIMi | 611669, gene |
neXtProti | NX_Q9NXH9 |
VEuPathDBi | HostDB:ENSG00000104907.12 |
Subcellular locationi
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: GO_Central
Pathology & Biotechi
Involvement in diseasei
Intellectual developmental disorder, autosomal recessive 68 (MRT68)3 Publications
The disease is caused by variants affecting the gene represented in this entry.
Disease descriptionA form of mental retardation, a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period.
Related information in OMIMKeywords - Diseasei
Mental retardationOrganism-specific databases
DisGeNETi | 55621 |
MalaCardsi | TRMT1 |
MIMi | 618302, phenotype |
OpenTargetsi | ENSG00000104907 |
Orphaneti | 528084, Non-specific syndromic intellectual disability |
PharmGKBi | PA134867808 |
Miscellaneous databases
Pharosi | Q9NXH9, Tbio |
Genetic variation databases
BioMutai | TRMT1 |
DMDMi | 12643821 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000147671 | 1 – 659 | tRNA (guanine(26)-N(2))-dimethyltransferaseAdd BLAST | 659 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 120 | PhosphoserineBy similarity | 1 | |
Modified residuei | 517 | PhosphoserineCombined sources | 1 | |
Modified residuei | 625 | PhosphoserineCombined sources | 1 | |
Modified residuei | 628 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 646 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9NXH9 |
jPOSTi | Q9NXH9 |
MassIVEi | Q9NXH9 |
MaxQBi | Q9NXH9 |
PaxDbi | Q9NXH9 |
PeptideAtlasi | Q9NXH9 |
PRIDEi | Q9NXH9 |
ProteomicsDBi | 83100 [Q9NXH9-1] 83101 [Q9NXH9-2] |
PTM databases
iPTMneti | Q9NXH9 |
PhosphoSitePlusi | Q9NXH9 |
Expressioni
Gene expression databases
Bgeei | ENSG00000104907, Expressed in lower esophagus mucosa and 194 other tissues |
ExpressionAtlasi | Q9NXH9, baseline and differential |
Genevisiblei | Q9NXH9, HS |
Organism-specific databases
HPAi | ENSG00000104907, Low tissue specificity |
Interactioni
Binary interactionsi
Hide detailsQ9NXH9
With | #Exp. | IntAct |
---|---|---|
MAGEA11 [P43364] | 5 | EBI-748900,EBI-739552 |
MAGEA11 - isoform 2 [P43364-2] | 3 | EBI-748900,EBI-10178634 |
TERF1 [P54274] | 2 | EBI-748900,EBI-710997 |
Protein-protein interaction databases
BioGRIDi | 120760, 70 interactors |
IntActi | Q9NXH9, 31 interactors |
MINTi | Q9NXH9 |
STRINGi | 9606.ENSP00000466967 |
Miscellaneous databases
RNActi | Q9NXH9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 55 – 499 | Trm1 methyltransferasePROSITE-ProRule annotationAdd BLAST | 445 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.PROSITE-ProRule annotation
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 600 – 627 | C3H1-typePROSITE-ProRule annotationAdd BLAST | 28 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG1253, Eukaryota |
GeneTreei | ENSGT00530000063646 |
HOGENOMi | CLU_010862_4_1_1 |
InParanoidi | Q9NXH9 |
OMAi | YRVSYSH |
PhylomeDBi | Q9NXH9 |
TreeFami | TF300851 |
Family and domain databases
Gene3Di | 3.30.56.70, 1 hit |
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR002905, Trm1 IPR042296, tRNA_met_Trm1_C IPR000571, Znf_CCCH IPR036855, Znf_CCCH_sf |
PANTHERi | PTHR10631, PTHR10631, 1 hit |
Pfami | View protein in Pfam PF02005, TRM, 1 hit PF00642, zf-CCCH, 1 hit |
SMARTi | View protein in SMART SM00356, ZnF_C3H1, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit SSF90229, SSF90229, 1 hit |
TIGRFAMsi | TIGR00308, TRM1, 1 hit |
PROSITEi | View protein in PROSITE PS51626, SAM_MT_TRM1, 1 hit PS50103, ZF_C3H1, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9NXH9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MQGSSLWLSL TFRSARVLSR ARFFEWQSPG LPNTAAMENG TGPYGEERPR
60 70 80 90 100
EVQETTVTEG AAKIAFPSAN EVFYNPVQEF NRDLTCAVIT EFARIQLGAK
110 120 130 140 150
GIQIKVPGEK DTQKVVVDLS EQEEEKVELK ESENLASGDQ PRTAAVGEIC
160 170 180 190 200
EEGLHVLEGL AASGLRSIRF ALEVPGLRSV VANDASTRAV DLIRRNVQLN
210 220 230 240 250
DVAHLVQPSQ ADARMLMYQH QRVSERFDVI DLDPYGSPAT FLDAAVQAVS
260 270 280 290 300
EGGLLCVTCT DMAVLAGNSG ETCYSKYGAM ALKSRACHEM ALRIVLHSLD
310 320 330 340 350
LRANCYQRFV VPLLSISADF YVRVFVRVFT GQAKVKASAS KQALVFQCVG
360 370 380 390 400
CGAFHLQRLG KASGVPSGRA KFSAACGPPV TPECEHCGQR HQLGGPMWAE
410 420 430 440 450
PIHDLDFVGR VLEAVSANPG RFHTSERIRG VLSVITEELP DVPLYYTLDQ
460 470 480 490 500
LSSTIHCNTP SLLQLRSALL HADFRVSLSH ACKNAVKTDA PASALWDIMR
510 520 530 540 550
CWEKECPVKR ERLSETSPAF RILSVEPRLQ ANFTIREDAN PSSRQRGLKR
560 570 580 590 600
FQANPEANWG PRPRARPGGK AADEAMEERR RLLQNKRKEP PEDVAQRAAR
610 620 630 640 650
LKTFPCKRFK EGTCQRGDQC CYSHSPPTPR VSADAAPDCP ETSNQTPPGP
GAAAGPGID
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7EJX9 | K7EJX9_HUMAN | tRNA (guanine(26)-N(2))-dimethyltra... | TRMT1 | 191 | Annotation score: | ||
K7EQQ8 | K7EQQ8_HUMAN | tRNA (guanine(26)-N(2))-dimethyltra... | TRMT1 | 297 | Annotation score: | ||
K7EQU7 | K7EQU7_HUMAN | tRNA (guanine(26)-N(2))-dimethyltra... | TRMT1 | 145 | Annotation score: | ||
K7ENI9 | K7ENI9_HUMAN | tRNA (guanine(26)-N(2))-dimethyltra... | TRMT1 | 105 | Annotation score: | ||
K7EQY6 | K7EQY6_HUMAN | tRNA (guanine(26)-N(2))-dimethyltra... | TRMT1 | 85 | Annotation score: | ||
K7ERR5 | K7ERR5_HUMAN | tRNA (guanine(26)-N(2))-dimethyltra... | TRMT1 | 113 | Annotation score: | ||
K7EMZ2 | K7EMZ2_HUMAN | tRNA (guanine(26)-N(2))-dimethyltra... | TRMT1 | 101 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 500 – 503 | RCWE → Q in AAC33150 (PubMed:15057824).Curated | 4 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016720 | 340 – 368 | Missing in isoform 2. 1 PublicationAdd BLAST | 29 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF196479 mRNA Translation: AAG28495.1 AK000251 mRNA Translation: BAA91031.1 AC005546 Genomic DNA Translation: AAC33150.1 BC002492 mRNA Translation: AAH02492.1 BC018302 mRNA Translation: AAH18302.1 BC040126 mRNA Translation: AAH40126.1 |
CCDSi | CCDS12293.1 [Q9NXH9-1] CCDS45997.1 [Q9NXH9-2] |
RefSeqi | NP_001129507.1, NM_001136035.2 [Q9NXH9-1] NP_001136026.1, NM_001142554.1 [Q9NXH9-2] NP_060192.1, NM_017722.3 [Q9NXH9-1] XP_016882433.1, XM_017026944.1 XP_016882434.1, XM_017026945.1 |
Genome annotation databases
Ensembli | ENST00000221504; ENSP00000221504; ENSG00000104907 [Q9NXH9-2] ENST00000357720; ENSP00000350352; ENSG00000104907 [Q9NXH9-1] ENST00000437766; ENSP00000416149; ENSG00000104907 [Q9NXH9-1] ENST00000592062; ENSP00000466967; ENSG00000104907 [Q9NXH9-1] |
GeneIDi | 55621 |
KEGGi | hsa:55621 |
UCSCi | uc002mwj.3, human [Q9NXH9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF196479 mRNA Translation: AAG28495.1 AK000251 mRNA Translation: BAA91031.1 AC005546 Genomic DNA Translation: AAC33150.1 BC002492 mRNA Translation: AAH02492.1 BC018302 mRNA Translation: AAH18302.1 BC040126 mRNA Translation: AAH40126.1 |
CCDSi | CCDS12293.1 [Q9NXH9-1] CCDS45997.1 [Q9NXH9-2] |
RefSeqi | NP_001129507.1, NM_001136035.2 [Q9NXH9-1] NP_001136026.1, NM_001142554.1 [Q9NXH9-2] NP_060192.1, NM_017722.3 [Q9NXH9-1] XP_016882433.1, XM_017026944.1 XP_016882434.1, XM_017026945.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 120760, 70 interactors |
IntActi | Q9NXH9, 31 interactors |
MINTi | Q9NXH9 |
STRINGi | 9606.ENSP00000466967 |
PTM databases
iPTMneti | Q9NXH9 |
PhosphoSitePlusi | Q9NXH9 |
Genetic variation databases
BioMutai | TRMT1 |
DMDMi | 12643821 |
Proteomic databases
EPDi | Q9NXH9 |
jPOSTi | Q9NXH9 |
MassIVEi | Q9NXH9 |
MaxQBi | Q9NXH9 |
PaxDbi | Q9NXH9 |
PeptideAtlasi | Q9NXH9 |
PRIDEi | Q9NXH9 |
ProteomicsDBi | 83100 [Q9NXH9-1] 83101 [Q9NXH9-2] |
Protocols and materials databases
Antibodypediai | 13510, 149 antibodies |
DNASUi | 55621 |
Genome annotation databases
Ensembli | ENST00000221504; ENSP00000221504; ENSG00000104907 [Q9NXH9-2] ENST00000357720; ENSP00000350352; ENSG00000104907 [Q9NXH9-1] ENST00000437766; ENSP00000416149; ENSG00000104907 [Q9NXH9-1] ENST00000592062; ENSP00000466967; ENSG00000104907 [Q9NXH9-1] |
GeneIDi | 55621 |
KEGGi | hsa:55621 |
UCSCi | uc002mwj.3, human [Q9NXH9-1] |
Organism-specific databases
CTDi | 55621 |
DisGeNETi | 55621 |
GeneCardsi | TRMT1 |
HGNCi | HGNC:25980, TRMT1 |
HPAi | ENSG00000104907, Low tissue specificity |
MalaCardsi | TRMT1 |
MIMi | 611669, gene 618302, phenotype |
neXtProti | NX_Q9NXH9 |
OpenTargetsi | ENSG00000104907 |
Orphaneti | 528084, Non-specific syndromic intellectual disability |
PharmGKBi | PA134867808 |
VEuPathDBi | HostDB:ENSG00000104907.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1253, Eukaryota |
GeneTreei | ENSGT00530000063646 |
HOGENOMi | CLU_010862_4_1_1 |
InParanoidi | Q9NXH9 |
OMAi | YRVSYSH |
PhylomeDBi | Q9NXH9 |
TreeFami | TF300851 |
Enzyme and pathway databases
PathwayCommonsi | Q9NXH9 |
Reactomei | R-HSA-6782315, tRNA modification in the nucleus and cytosol |
Miscellaneous databases
BioGRID-ORCSi | 55621, 20 hits in 874 CRISPR screens |
ChiTaRSi | TRMT1, human |
GenomeRNAii | 55621 |
Pharosi | Q9NXH9, Tbio |
PROi | PR:Q9NXH9 |
RNActi | Q9NXH9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000104907, Expressed in lower esophagus mucosa and 194 other tissues |
ExpressionAtlasi | Q9NXH9, baseline and differential |
Genevisiblei | Q9NXH9, HS |
Family and domain databases
Gene3Di | 3.30.56.70, 1 hit |
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR002905, Trm1 IPR042296, tRNA_met_Trm1_C IPR000571, Znf_CCCH IPR036855, Znf_CCCH_sf |
PANTHERi | PTHR10631, PTHR10631, 1 hit |
Pfami | View protein in Pfam PF02005, TRM, 1 hit PF00642, zf-CCCH, 1 hit |
SMARTi | View protein in SMART SM00356, ZnF_C3H1, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit SSF90229, SSF90229, 1 hit |
TIGRFAMsi | TIGR00308, TRM1, 1 hit |
PROSITEi | View protein in PROSITE PS51626, SAM_MT_TRM1, 1 hit PS50103, ZF_C3H1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRM1_HUMAN | |
Accessioni | Q9NXH9Primary (citable) accession number: Q9NXH9 Secondary accession number(s): O76103, Q548Y5, Q8WVA6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | October 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families