UniProtKB - Q9NXF8 (ZDHC7_HUMAN)
Protein
Palmitoyltransferase ZDHHC7
Gene
ZDHHC7
Organism
Homo sapiens (Human)
Status
Functioni
Golgi-localized palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates and therefore functions in several unrelated biological processes (PubMed:22031296, PubMed:27380321, PubMed:28196865). Has no stringent fatty acid selectivity and in addition to palmitate can also transfer onto target proteins myristate from tetradecanoyl-CoA and stearate from octadecanoyl-CoA (By similarity). Palmitoylates sex steroid hormone receptors, including ESR1, PGR and AR, thereby regulating their targeting to the plasma membrane and their function in rapid intracellular signaling upon binding of sex hormones (PubMed:22031296). Palmitoylates GNAQ, a heterotrimeric G protein, regulating its dynamic localization at the plasma membrane and is thereby involved in GNAQ-dependent G protein-coupled receptor signaling pathways (PubMed:19001095). Functions also in ligand-induced cell death by regulating the FAS signaling pathway through the palmitoylation and stabilization of the receptor at the plasma membrane (PubMed:25301068). In epithelial cells, palmitoylates SCRIB and regulates its localization to the plasma membrane, regulating indirectly cell polarity and differentiation (PubMed:27380321). Also palmitoylates JAM3 and promotes its expression at tight junctions and regulates its function in cell migration (PubMed:28196865). Palmitoylates the glucose transporter GLUT4/SLC2A4 and controls the insulin-dependent translocation of GLUT4 to the plasma membrane (By similarity). In brain, could also palmitoylate SNAP25 and DLG4/PSD95 (By similarity). Could also palmitoylate DNAJC5 and regulate its localization to the Golgi membrane (By similarity). Could also palmitoylate NCDN (By similarity). May play a role in follicle stimulation hormone (FSH) activation of testicular Sertoli cells (By similarity).By similarity5 Publications
Catalytic activityi
- EC:2.3.1.2254 PublicationsThis reaction proceeds in the forward1 Publication direction.
- L-cysteinyl-[protein] + tetradecanoyl-CoA = CoA + S-tetradecanoyl-L-cysteinyl-[protein]By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 160 | S-palmitoyl cysteine intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- palmitoyltransferase activity Source: UniProtKB
- protein-cysteine S-palmitoyltransferase activity Source: UniProtKB
GO - Biological processi
- glucose import in response to insulin stimulus Source: UniProtKB
- negative regulation of catabolic process Source: UniProtKB
- peptidyl-L-cysteine S-palmitoylation Source: UniProtKB
- polarized epithelial cell differentiation Source: UniProtKB
- protein palmitoylation Source: UniProtKB
- protein targeting to membrane Source: GO_Central
- regulation of Fas signaling pathway Source: UniProtKB
- regulation of G protein-coupled receptor signaling pathway Source: UniProtKB
- regulation of protein localization to cell-cell junction Source: UniProtKB
- regulation of protein localization to plasma membrane Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
PathwayCommonsi | Q9NXF8 |
Reactomei | R-HSA-9009391, Extra-nuclear estrogen signaling |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ZDHHC7Imported |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000153786.12 |
HGNCi | HGNC:18459, ZDHHC7 |
MIMi | 614604, gene |
neXtProti | NX_Q9NXF8 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi membrane Source: UniProtKB-SubCell
Nucleus
- nucleoplasm Source: HPA
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 50 | CytoplasmicCuratedAdd BLAST | 50 | |
Transmembranei | 51 – 71 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 72 – 75 | LumenalCurated | 4 | |
Transmembranei | 76 – 96 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 97 – 173 | CytoplasmicCuratedAdd BLAST | 77 | |
Transmembranei | 174 – 194 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 195 – 217 | LumenalCuratedAdd BLAST | 23 | |
Transmembranei | 218 – 238 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 239 – 308 | CytoplasmicCuratedAdd BLAST | 70 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 55625 |
OpenTargetsi | ENSG00000153786 |
PharmGKBi | PA38335 |
Miscellaneous databases
Pharosi | Q9NXF8, Tbio |
Polymorphism and mutation databases
BioMutai | ZDHHC7 |
DMDMi | 116242853 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000212874 | 1 – 308 | Palmitoyltransferase ZDHHC7Add BLAST | 308 |
Post-translational modificationi
Autopalmitoylated.By similarity
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
EPDi | Q9NXF8 |
jPOSTi | Q9NXF8 |
MassIVEi | Q9NXF8 |
MaxQBi | Q9NXF8 |
PeptideAtlasi | Q9NXF8 |
PRIDEi | Q9NXF8 |
ProteomicsDBi | 83089 [Q9NXF8-1] 83090 [Q9NXF8-2] |
PTM databases
iPTMneti | Q9NXF8 |
PhosphoSitePlusi | Q9NXF8 |
SwissPalmi | Q9NXF8 |
Expressioni
Gene expression databases
Bgeei | ENSG00000153786, Expressed in secondary oocyte and 250 other tissues |
ExpressionAtlasi | Q9NXF8, baseline and differential |
Genevisiblei | Q9NXF8, HS |
Organism-specific databases
HPAi | ENSG00000153786, Low tissue specificity |
Interactioni
Subunit structurei
Homooligomers. Heterooligomers with ZDHHC3.
By similarityBinary interactionsi
Hide detailsIsoform 2 [Q9NXF8-2]
With | #Exp. | IntAct |
---|---|---|
CYB561 [P49447] | 3 | EBI-12948063,EBI-8646596 |
LAT - isoform 2 [O43561-2] | 3 | EBI-12948063,EBI-8070286 |
YIPF1 [Q9Y548] | 3 | EBI-12948063,EBI-7850136 |
Protein-protein interaction databases
BioGRIDi | 120764, 15 interactors |
IntActi | Q9NXF8, 9 interactors |
Miscellaneous databases
RNActi | Q9NXF8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 130 – 180 | DHHCPROSITE-ProRule annotationAdd BLAST | 51 |
Domaini
The DHHC domain is required for palmitoyltransferase activity.By similarity
Sequence similaritiesi
Belongs to the DHHC palmitoyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
GeneTreei | ENSGT00940000156519 |
HOGENOMi | CLU_048061_1_1_1 |
InParanoidi | Q9NXF8 |
OMAi | ATKEYMD |
OrthoDBi | 1491968at2759 |
PhylomeDBi | Q9NXF8 |
TreeFami | TF319798 |
Family and domain databases
InterProi | View protein in InterPro IPR001594, Palmitoyltrfase_DHHC |
Pfami | View protein in Pfam PF01529, DHHC, 1 hit |
PROSITEi | View protein in PROSITE PS50216, DHHC, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9NXF8-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MQPSGHRLRD VEHHPLLAEN DNYDSSSSSS SEADVADRVW FIRDGCGMIC
60 70 80 90 100
AVMTWLLVAY ADFVVTFVML LPSKDFWYSV VNGVIFNCLA VLALSSHLRT
110 120 130 140 150
MLTDPGAVPK GNATKEYMES LQLKPGEVIY KCPKCCCIKP ERAHHCSICK
160 170 180 190 200
RCIRKMDHHC PWVNNCVGEK NQRFFVLFTM YIALSSVHAL ILCGFQFISC
210 220 230 240 250
VRGQWTECSD FSPPITVILL IFLCLEGLLF FTFTAVMFGT QIHSICNDET
260 270 280 290 300
EIERLKSEKP TWERRLRWEG MKSVFGGPPS LLWMNPFVGF RFRRLPTRPR
KGGPEFSV
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BNQ9 | H3BNQ9_HUMAN | Palmitoyltransferase | ZDHHC7 | 141 | Annotation score: | ||
H3BMI0 | H3BMI0_HUMAN | Palmitoyltransferase ZDHHC7 | ZDHHC7 | 95 | Annotation score: |
Sequence cautioni
The sequence AAH17702 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAA91814 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 22 | N → D in BAA91055 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_036261 | 44 | D → N in a colorectal cancer sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_028360 | 201 | V → I. Corresponds to variant dbSNP:rs13334011Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_006942 | 105 | P → PEKSSDCRPSACTVKTGLDP TLVGICGEGTESVQSLLL in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK000286 mRNA Translation: BAA91055.1 AK001654 mRNA Translation: BAA91814.1 Different initiation. CH471114 Genomic DNA Translation: EAW95464.1 CH471114 Genomic DNA Translation: EAW95465.1 BC017702 mRNA Translation: AAH17702.1 Different initiation. BC018772 mRNA Translation: AAH18772.1 |
CCDSi | CCDS10950.1 [Q9NXF8-1] CCDS45538.1 [Q9NXF8-2] |
RefSeqi | NP_001139020.1, NM_001145548.1 [Q9NXF8-2] NP_060210.2, NM_017740.2 [Q9NXF8-1] |
Genome annotation databases
Ensembli | ENST00000313732; ENSP00000315604; ENSG00000153786 [Q9NXF8-1] ENST00000344861; ENSP00000341681; ENSG00000153786 [Q9NXF8-2] ENST00000564466; ENSP00000456782; ENSG00000153786 [Q9NXF8-2] |
GeneIDi | 55625 |
KEGGi | hsa:55625 |
UCSCi | uc002fiq.3, human [Q9NXF8-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK000286 mRNA Translation: BAA91055.1 AK001654 mRNA Translation: BAA91814.1 Different initiation. CH471114 Genomic DNA Translation: EAW95464.1 CH471114 Genomic DNA Translation: EAW95465.1 BC017702 mRNA Translation: AAH17702.1 Different initiation. BC018772 mRNA Translation: AAH18772.1 |
CCDSi | CCDS10950.1 [Q9NXF8-1] CCDS45538.1 [Q9NXF8-2] |
RefSeqi | NP_001139020.1, NM_001145548.1 [Q9NXF8-2] NP_060210.2, NM_017740.2 [Q9NXF8-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 120764, 15 interactors |
IntActi | Q9NXF8, 9 interactors |
PTM databases
iPTMneti | Q9NXF8 |
PhosphoSitePlusi | Q9NXF8 |
SwissPalmi | Q9NXF8 |
Polymorphism and mutation databases
BioMutai | ZDHHC7 |
DMDMi | 116242853 |
Proteomic databases
EPDi | Q9NXF8 |
jPOSTi | Q9NXF8 |
MassIVEi | Q9NXF8 |
MaxQBi | Q9NXF8 |
PeptideAtlasi | Q9NXF8 |
PRIDEi | Q9NXF8 |
ProteomicsDBi | 83089 [Q9NXF8-1] 83090 [Q9NXF8-2] |
Protocols and materials databases
Antibodypediai | 30621, 135 antibodies |
DNASUi | 55625 |
Genome annotation databases
Ensembli | ENST00000313732; ENSP00000315604; ENSG00000153786 [Q9NXF8-1] ENST00000344861; ENSP00000341681; ENSG00000153786 [Q9NXF8-2] ENST00000564466; ENSP00000456782; ENSG00000153786 [Q9NXF8-2] |
GeneIDi | 55625 |
KEGGi | hsa:55625 |
UCSCi | uc002fiq.3, human [Q9NXF8-1] |
Organism-specific databases
CTDi | 55625 |
DisGeNETi | 55625 |
EuPathDBi | HostDB:ENSG00000153786.12 |
GeneCardsi | ZDHHC7 |
HGNCi | HGNC:18459, ZDHHC7 |
HPAi | ENSG00000153786, Low tissue specificity |
MIMi | 614604, gene |
neXtProti | NX_Q9NXF8 |
OpenTargetsi | ENSG00000153786 |
PharmGKBi | PA38335 |
GenAtlasi | Search... |
Phylogenomic databases
GeneTreei | ENSGT00940000156519 |
HOGENOMi | CLU_048061_1_1_1 |
InParanoidi | Q9NXF8 |
OMAi | ATKEYMD |
OrthoDBi | 1491968at2759 |
PhylomeDBi | Q9NXF8 |
TreeFami | TF319798 |
Enzyme and pathway databases
PathwayCommonsi | Q9NXF8 |
Reactomei | R-HSA-9009391, Extra-nuclear estrogen signaling |
Miscellaneous databases
BioGRID-ORCSi | 55625, 16 hits in 846 CRISPR screens |
ChiTaRSi | ZDHHC7, human |
GenomeRNAii | 55625 |
Pharosi | Q9NXF8, Tbio |
PROi | PR:Q9NXF8 |
RNActi | Q9NXF8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000153786, Expressed in secondary oocyte and 250 other tissues |
ExpressionAtlasi | Q9NXF8, baseline and differential |
Genevisiblei | Q9NXF8, HS |
Family and domain databases
InterProi | View protein in InterPro IPR001594, Palmitoyltrfase_DHHC |
Pfami | View protein in Pfam PF01529, DHHC, 1 hit |
PROSITEi | View protein in PROSITE PS50216, DHHC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZDHC7_HUMAN | |
Accessioni | Q9NXF8Primary (citable) accession number: Q9NXF8 Secondary accession number(s): D3DUM1, Q8WV42, Q9NVD8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2003 |
Last sequence update: | October 17, 2006 | |
Last modified: | December 2, 2020 | |
This is version 156 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations