MED29

Functionⁱ

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.1 Publication

GO - Biological processⁱ

regulation of transcription, DNA-templated Source: UniProtKB-KW
transcription, DNA-templated Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator
Biological process	Transcription, Transcription regulation

Enzyme and pathway databases

Reactomeⁱ	R-HSA-1989781 PPARA activates gene expression R-HSA-381340 Transcriptional regulation of white adipocyte differentiation

Reactomeⁱ

R-HSA-1989781 PPARA activates gene expression
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Mediator of RNA polymerase II transcription subunit 29 Alternative name(s): Intersex-like protein Mediator complex subunit 29
Gene namesⁱ	Name:MED29 Synonyms:IXL
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 19

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000063322.13
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:23074 MED29
MIMⁱ	612914 gene
neXtProtⁱ	NX_Q9NX70

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	55588
Open Targets More...OpenTargetsⁱ	ENSG00000063322
PharmGKBⁱ	PA162395655

Polymorphism and mutation databases

DMDMⁱ	74734688

DMDMⁱ

74734688

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedCombined sources
ChainⁱPRO_0000288058	2 – 200	Mediator of RNA polymerase II transcription subunit 29Add BLAST		199

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	2	N-acetylalanineCombined sources		1

Keywords - PTMⁱ

Acetylation

Proteomic databases

EPDⁱ	Q9NX70
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9NX70
PaxDbⁱ	Q9NX70
PeptideAtlas More...PeptideAtlasⁱ	Q9NX70
PRIDEⁱ	Q9NX70
ProteomicsDBⁱ	83050

PTM databases

iPTMnetⁱ	Q9NX70
PhosphoSitePlusⁱ	Q9NX70

Expressionⁱ

Tissue specificityⁱ

Widely expressed in embryo and adult.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000063322 Expressed in 197 organ(s), highest expression level in prefrontal cortex
CleanExⁱ	HS_MED29
ExpressionAtlasⁱ	Q9NX70 baseline and differential
Genevisibleⁱ	Q9NX70 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA055956

HPAⁱ

HPA055956

Interactionⁱ

Subunit structureⁱ

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Associates with the MED18/MED20 heteromer.4 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Med11	Q9D8C6	2	EBI-394656,EBI-6260909	From Mus musculus.
MED18	Q9BUE0	5	EBI-394656,EBI-394640
MED19	A0JLT2	8	EBI-394656,EBI-394430
MED20	Q9H944	5	EBI-394656,EBI-394644
Med20	Q9R0X0	2	EBI-394656,EBI-398698	From Mus musculus.
MED22	Q15528	4	EBI-394656,EBI-394687
MED28	Q9H204	3	EBI-394656,EBI-514199
Med30	Q9CQI9	2	EBI-394656,EBI-309220	From Mus musculus.
MED6	O75586	3	EBI-394656,EBI-394624
Med8	Q9D7W5	2	EBI-394656,EBI-7990252	From Mus musculus.

Protein-protein interaction databases

BioGridⁱ	120734, 71 interactors
ComplexPortalⁱ	CPX-3227 Core mediator complex
CORUMⁱ	Q9NX70
IntActⁱ	Q9NX70, 95 interactors
STRINGⁱ	9606.ENSP00000314343

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9NX70
SMRⁱ	Q9NX70
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	29 – 84	Gln-richAdd BLAST		56

Sequence similaritiesⁱ

Belongs to the Mediator complex subunit 29 family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG410IXGN Eukaryota ENOG4111K6Y LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000007540
HOVERGENⁱ	HBG060489
InParanoidⁱ	Q9NX70
KEGG Orthology (KO) More...KOⁱ	K15142
PhylomeDBⁱ	Q9NX70
TreeFamⁱ	TF326632

Family and domain databases

InterProⁱ	View protein in InterPro IPR021018 Mediator_Med29_met
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF11568 Med29, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q9NX70-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASQQQASA ASSAAGVSGP SSAGGPGPQQ QPQPPAQLVG PAQSGLLQQQ 
        60         70         80         90        100
QQDFDPVQRY KMLIPQLKES LQTLMKVAAQ NLIQNTNIDN GQKSSDGPIQ 
       110        120        130        140        150
RFDKCLEEFY ALCDQLELCL RLAHECLSQS CDSAKHSPTL VPTATKPDAV 
       160        170        180        190        200
QPDSLPYPQY LAVIKAQISC AKDIHTALLD CANKVTGKTP APPAGPGGTL

Length:200

Mass (Da):21,073

Last modified:October 1, 2000 - v1

Checksum:ⁱE0CDA1DDD89635CA

GO

Isoform 2 (identifier: Q9NX70-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
121-196: RLAHECLSQS...GKTPAPPAGP → PPSPTQCSLT...QWLVGGVQRE
197-200: Missing.

« Hide

        10         20         30         40         50
MAASQQQASA ASSAAGVSGP SSAGGPGPQQ QPQPPAQLVG PAQSGLLQQQ 
        60         70         80         90        100
QQDFDPVQRY KMLIPQLKES LQTLMKVAAQ NLIQNTNIDN GQKSSDGPIQ 
       110        120        130        140        150
RFDKCLEEFY ALCDQLELCL PPSPTQCSLT ASPTHSTWRS SKPRFPVPRT 
       160        170        180        190 
FTPPCWTVPT RSRARHPHHL LALGALCEVG DREWGRQWLV GGVQRE

Note: No experimental confirmation available.

Show »

Length:196

Mass (Da):21,362

Checksum:ⁱ5A3367EFACBD5356

GO

Computationally mapped potential isoform sequencesⁱ

There are 4 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

B4DUA7	B4DUA7_HUMAN	Intersex-like (Drosophila) Intersex-like (Drosophila) (Mediator of RNA polymerase II transcription subunit 29) (cDNA FLJ54860, highly similar to Homo sapiens intersex-lik (IXL), mRNA)	MED29 IXL, hCG_43365	221	Annotation score:
M0R0U2	M0R0U2_HUMAN	Mediator of RNA polymerase II trans... Mediator of RNA polymerase II transcription subunit 29	MED29	114	Annotation score:
M0R3F3	M0R3F3_HUMAN	Mediator of RNA polymerase II trans... Mediator of RNA polymerase II transcription subunit 29	MED29	208	Annotation score:
M0R0A0	M0R0A0_HUMAN	Mediator of RNA polymerase II trans... Mediator of RNA polymerase II transcription subunit 29	MED29	96	Annotation score:

Sequence cautionⁱ

The sequence AAU43732 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_056133	121 – 196	RLAHE…PPAGP → PPSPTQCSLTASPTHSTWRS SKPRFPVPRTFTPPCWTVPT RSRARHPHHLLALGALCEVG DREWGRQWLVGGVQRE in isoform 2. 1 PublicationAdd BLAST		76
Alternative sequenceⁱVSP_056134	197 – 200	Missing in isoform 2. 1 Publication		4

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY729650 mRNA Translation: AAU43732.1 Different initiation. AK000411 mRNA Translation: BAA91147.1 AK298014 mRNA Translation: BAG60318.1 AL137304 mRNA Translation: CAB70687.2 AC005239 Genomic DNA No translation available. AC011500 Genomic DNA No translation available. AC104106 Genomic DNA No translation available. BC019015 mRNA Translation: AAH19015.1
PIRⁱ	T46497
NCBI Reference Sequences More...RefSeqⁱ	NP_001304699.1, NM_001317770.1 NP_060062.1, NM_017592.2
UniGeneⁱ	Hs.611541

Genome annotation databases

Ensemblⁱ	ENST00000594368; ENSP00000472501; ENSG00000063322 [Q9NX70-2] ENST00000599213; ENSP00000471802; ENSG00000063322 [Q9NX70-1]
GeneIDⁱ	55588
KEGGⁱ	hsa:55588
UCSC genome browser More...UCSCⁱ	uc060ykk.1 human [Q9NX70-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9NX70	Mediator of RNA polymerase II transcription subunit 29		PONAB		200	UniRef100_Q9NX70
Intersex-like (Drosophila)		HUMAN		221
Mediator complex subunit 29		GORGO		221
Macaca fascicularis brain cDNA clone: QflA-21094, similar to human homolog of Drosophila Intersex (Intersex), mRNA, RefSeq: XM_290829.3		MACFA		200

Protein	Similar proteins		Species	Score	Length	Source
Q9NX70	Mediator of RNA polymerase II transcription subunit 29		PONAB		200	UniRef90_Q9NX70
Mediator of RNA polymerase II transcription subunit 29		BOVIN		200
Mediator of RNA polymerase II transcription subunit 29		MOUSE		199
Intersex-like (Drosophila)		HUMAN		221
Mediator complex subunit 29		GORGO		221
+113
Q9NX70-2	MED29 isoform 5		PANTR		196	UniRef90_Q9NX70-2
MED29 isoform 5		PONAB		196
Uncharacterized protein		PAPAN		196
Mediator complex subunit 29		CALJA		195
Uncharacterized protein		MACMU		191
+5

Protein	Similar proteins		Species	Score	Length	Source
Q9NX70	Mediator of RNA polymerase II transcription subunit 29		PONAB		200	UniRef50_Q9NX70
Mediator of RNA polymerase II transcription subunit 29		BOVIN		200
Mediator of RNA polymerase II transcription subunit 29		MOUSE		199
Mediator of RNA polymerase II transcription subunit 29		XENTR		188
Mediator of RNA polymerase II transcription subunit 29		XENLA		186
+230

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY729650 mRNA Translation: AAU43732.1 Different initiation. AK000411 mRNA Translation: BAA91147.1 AK298014 mRNA Translation: BAG60318.1 AL137304 mRNA Translation: CAB70687.2 AC005239 Genomic DNA No translation available. AC011500 Genomic DNA No translation available. AC104106 Genomic DNA No translation available. BC019015 mRNA Translation: AAH19015.1
PIRⁱ	T46497
RefSeqⁱ	NP_001304699.1, NM_001317770.1 NP_060062.1, NM_017592.2
UniGeneⁱ	Hs.611541

3D structure databases

ProteinModelPortalⁱ	Q9NX70
SMRⁱ	Q9NX70
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	120734, 71 interactors
ComplexPortalⁱ	CPX-3227 Core mediator complex
CORUMⁱ	Q9NX70
IntActⁱ	Q9NX70, 95 interactors
STRINGⁱ	9606.ENSP00000314343

PTM databases

iPTMnetⁱ	Q9NX70
PhosphoSitePlusⁱ	Q9NX70

Polymorphism and mutation databases

DMDMⁱ	74734688

DMDMⁱ

74734688

Proteomic databases

EPDⁱ	Q9NX70
MaxQBⁱ	Q9NX70
PaxDbⁱ	Q9NX70
PeptideAtlasⁱ	Q9NX70
PRIDEⁱ	Q9NX70
ProteomicsDBⁱ	83050

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENST00000594368; ENSP00000472501; ENSG00000063322 [Q9NX70-2] ENST00000599213; ENSP00000471802; ENSG00000063322 [Q9NX70-1]
GeneIDⁱ	55588
KEGGⁱ	hsa:55588
UCSCⁱ	uc060ykk.1 human [Q9NX70-1]

Organism-specific databases

CTDⁱ	55588
DisGeNETⁱ	55588
EuPathDBⁱ	HostDB:ENSG00000063322.13
GeneCardsⁱ	MED29
H-InvDBⁱ	HIX0015117
HGNCⁱ	HGNC:23074 MED29
HPAⁱ	HPA055956
MIMⁱ	612914 gene
neXtProtⁱ	NX_Q9NX70
OpenTargetsⁱ	ENSG00000063322
PharmGKBⁱ	PA162395655
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	ENOG410IXGN Eukaryota ENOG4111K6Y LUCA
GeneTreeⁱ	ENSGT00390000007540
HOVERGENⁱ	HBG060489
InParanoidⁱ	Q9NX70
KOⁱ	K15142
PhylomeDBⁱ	Q9NX70
TreeFamⁱ	TF326632

Enzyme and pathway databases

Reactomeⁱ	R-HSA-1989781 PPARA activates gene expression R-HSA-381340 Transcriptional regulation of white adipocyte differentiation

Reactomeⁱ

R-HSA-1989781 PPARA activates gene expression
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation

Miscellaneous databases

ChiTaRSⁱ	MED29 human
GenomeRNAiⁱ	55588
Protein Ontology More...PROⁱ	PR:Q9NX70
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000063322 Expressed in 197 organ(s), highest expression level in prefrontal cortex
CleanExⁱ	HS_MED29
ExpressionAtlasⁱ	Q9NX70 baseline and differential
Genevisibleⁱ	Q9NX70 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR021018 Mediator_Med29_met
Pfamⁱ	View protein in Pfam PF11568 Med29, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MED29_HUMAN
Accessionⁱ	Q9NX70Primary (citable) accession number: Q9NX70 Secondary accession number(s): B4DNQ6 , M0R2E4, Q5XX09, Q9NTF4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
	Last sequence update:	October 1, 2000
	Last modified:	September 12, 2018
	This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

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Annotation systems

Supporting data

UniProtKB - Q9NX70 (MED29_HUMAN)

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Mediator of RNA polymerase II transcription subunit 29

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ