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Protein

Mediator of RNA polymerase II transcription subunit 29

Gene

MED29

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.1 Publication

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 29
Alternative name(s):
Intersex-like protein
Mediator complex subunit 29
Gene namesi
Name:MED29
Synonyms:IXL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000063322.13
HGNCiHGNC:23074 MED29
MIMi612914 gene
neXtProtiNX_Q9NX70

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55588
OpenTargetsiENSG00000063322
PharmGKBiPA162395655

Polymorphism and mutation databases

DMDMi74734688

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002880582 – 200Mediator of RNA polymerase II transcription subunit 29Add BLAST199

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9NX70
MaxQBiQ9NX70
PaxDbiQ9NX70
PeptideAtlasiQ9NX70
PRIDEiQ9NX70
ProteomicsDBi83050

PTM databases

iPTMnetiQ9NX70
PhosphoSitePlusiQ9NX70

Expressioni

Tissue specificityi

Widely expressed in embryo and adult.1 Publication

Gene expression databases

BgeeiENSG00000063322 Expressed in 197 organ(s), highest expression level in prefrontal cortex
CleanExiHS_MED29
ExpressionAtlasiQ9NX70 baseline and differential
GenevisibleiQ9NX70 HS

Organism-specific databases

HPAiHPA055956

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Associates with the MED18/MED20 heteromer.4 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi120734, 71 interactors
ComplexPortaliCPX-3227 Core mediator complex
CORUMiQ9NX70
IntActiQ9NX70, 95 interactors
STRINGi9606.ENSP00000314343

Structurei

3D structure databases

ProteinModelPortaliQ9NX70
SMRiQ9NX70
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi29 – 84Gln-richAdd BLAST56

Sequence similaritiesi

Belongs to the Mediator complex subunit 29 family.Curated

Phylogenomic databases

eggNOGiENOG410IXGN Eukaryota
ENOG4111K6Y LUCA
GeneTreeiENSGT00390000007540
HOVERGENiHBG060489
InParanoidiQ9NX70
KOiK15142
PhylomeDBiQ9NX70
TreeFamiTF326632

Family and domain databases

InterProiView protein in InterPro
IPR021018 Mediator_Med29_met
PfamiView protein in Pfam
PF11568 Med29, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9NX70-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAASQQQASA ASSAAGVSGP SSAGGPGPQQ QPQPPAQLVG PAQSGLLQQQ
60 70 80 90 100
QQDFDPVQRY KMLIPQLKES LQTLMKVAAQ NLIQNTNIDN GQKSSDGPIQ
110 120 130 140 150
RFDKCLEEFY ALCDQLELCL RLAHECLSQS CDSAKHSPTL VPTATKPDAV
160 170 180 190 200
QPDSLPYPQY LAVIKAQISC AKDIHTALLD CANKVTGKTP APPAGPGGTL
Length:200
Mass (Da):21,073
Last modified:October 1, 2000 - v1
Checksum:iE0CDA1DDD89635CA
GO
Isoform 2 (identifier: Q9NX70-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     121-196: RLAHECLSQS...GKTPAPPAGP → PPSPTQCSLT...QWLVGGVQRE
     197-200: Missing.

Note: No experimental confirmation available.
Show »
Length:196
Mass (Da):21,362
Checksum:i5A3367EFACBD5356
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B4DUA7B4DUA7_HUMAN
Intersex-like (Drosophila)
MED29 IXL, hCG_43365
221Annotation score:
M0R0U2M0R0U2_HUMAN
Mediator of RNA polymerase II trans...
MED29
114Annotation score:
M0R3F3M0R3F3_HUMAN
Mediator of RNA polymerase II trans...
MED29
208Annotation score:
M0R0A0M0R0A0_HUMAN
Mediator of RNA polymerase II trans...
MED29
96Annotation score:

Sequence cautioni

The sequence AAU43732 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056133121 – 196RLAHE…PPAGP → PPSPTQCSLTASPTHSTWRS SKPRFPVPRTFTPPCWTVPT RSRARHPHHLLALGALCEVG DREWGRQWLVGGVQRE in isoform 2. 1 PublicationAdd BLAST76
Alternative sequenceiVSP_056134197 – 200Missing in isoform 2. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY729650 mRNA Translation: AAU43732.1 Different initiation.
AK000411 mRNA Translation: BAA91147.1
AK298014 mRNA Translation: BAG60318.1
AL137304 mRNA Translation: CAB70687.2
AC005239 Genomic DNA No translation available.
AC011500 Genomic DNA No translation available.
AC104106 Genomic DNA No translation available.
BC019015 mRNA Translation: AAH19015.1
PIRiT46497
RefSeqiNP_001304699.1, NM_001317770.1
NP_060062.1, NM_017592.2
UniGeneiHs.611541

Genome annotation databases

EnsembliENST00000594368; ENSP00000472501; ENSG00000063322 [Q9NX70-2]
ENST00000599213; ENSP00000471802; ENSG00000063322 [Q9NX70-1]
GeneIDi55588
KEGGihsa:55588
UCSCiuc060ykk.1 human [Q9NX70-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY729650 mRNA Translation: AAU43732.1 Different initiation.
AK000411 mRNA Translation: BAA91147.1
AK298014 mRNA Translation: BAG60318.1
AL137304 mRNA Translation: CAB70687.2
AC005239 Genomic DNA No translation available.
AC011500 Genomic DNA No translation available.
AC104106 Genomic DNA No translation available.
BC019015 mRNA Translation: AAH19015.1
PIRiT46497
RefSeqiNP_001304699.1, NM_001317770.1
NP_060062.1, NM_017592.2
UniGeneiHs.611541

3D structure databases

ProteinModelPortaliQ9NX70
SMRiQ9NX70
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120734, 71 interactors
ComplexPortaliCPX-3227 Core mediator complex
CORUMiQ9NX70
IntActiQ9NX70, 95 interactors
STRINGi9606.ENSP00000314343

PTM databases

iPTMnetiQ9NX70
PhosphoSitePlusiQ9NX70

Polymorphism and mutation databases

DMDMi74734688

Proteomic databases

EPDiQ9NX70
MaxQBiQ9NX70
PaxDbiQ9NX70
PeptideAtlasiQ9NX70
PRIDEiQ9NX70
ProteomicsDBi83050

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000594368; ENSP00000472501; ENSG00000063322 [Q9NX70-2]
ENST00000599213; ENSP00000471802; ENSG00000063322 [Q9NX70-1]
GeneIDi55588
KEGGihsa:55588
UCSCiuc060ykk.1 human [Q9NX70-1]

Organism-specific databases

CTDi55588
DisGeNETi55588
EuPathDBiHostDB:ENSG00000063322.13
GeneCardsiMED29
H-InvDBiHIX0015117
HGNCiHGNC:23074 MED29
HPAiHPA055956
MIMi612914 gene
neXtProtiNX_Q9NX70
OpenTargetsiENSG00000063322
PharmGKBiPA162395655
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IXGN Eukaryota
ENOG4111K6Y LUCA
GeneTreeiENSGT00390000007540
HOVERGENiHBG060489
InParanoidiQ9NX70
KOiK15142
PhylomeDBiQ9NX70
TreeFamiTF326632

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation

Miscellaneous databases

ChiTaRSiMED29 human
GenomeRNAii55588
PROiPR:Q9NX70
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000063322 Expressed in 197 organ(s), highest expression level in prefrontal cortex
CleanExiHS_MED29
ExpressionAtlasiQ9NX70 baseline and differential
GenevisibleiQ9NX70 HS

Family and domain databases

InterProiView protein in InterPro
IPR021018 Mediator_Med29_met
PfamiView protein in Pfam
PF11568 Med29, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMED29_HUMAN
AccessioniPrimary (citable) accession number: Q9NX70
Secondary accession number(s): B4DNQ6
, M0R2E4, Q5XX09, Q9NTF4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 1, 2000
Last modified: September 12, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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