UniProtKB - Q9NX70 (MED29_HUMAN)
Mediator of RNA polymerase II transcription subunit 29
MED29
Functioni
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
1 PublicationGO - Molecular functioni
- transcription coregulator activity Source: GO_Central
GO - Biological processi
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q9NX70 |
Reactomei | R-HSA-1989781, PPARA activates gene expression R-HSA-381340, Transcriptional regulation of white adipocyte differentiation |
SignaLinki | Q9NX70 |
SIGNORi | Q9NX70 |
Names & Taxonomyi
Protein namesi | Recommended name: Mediator of RNA polymerase II transcription subunit 29Alternative name(s): Intersex-like protein Mediator complex subunit 29 |
Gene namesi | Name:MED29 Synonyms:IXL |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:23074, MED29 |
MIMi | 612914, gene |
neXtProti | NX_Q9NX70 |
VEuPathDBi | HostDB:ENSG00000063322 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- core mediator complex Source: ComplexPortal
- mediator complex Source: MGI
- nucleoplasm Source: HPA
- nucleus Source: ComplexPortal
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 55588 |
OpenTargetsi | ENSG00000063322 |
PharmGKBi | PA162395655 |
Miscellaneous databases
Pharosi | Q9NX70, Tbio |
Genetic variation databases
BioMutai | MED29 |
DMDMi | 74734688 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000288058 | 2 – 200 | Mediator of RNA polymerase II transcription subunit 29Add BLAST | 199 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q9NX70 |
jPOSTi | Q9NX70 |
MassIVEi | Q9NX70 |
MaxQBi | Q9NX70 |
PaxDbi | Q9NX70 |
PeptideAtlasi | Q9NX70 |
PRIDEi | Q9NX70 |
ProteomicsDBi | 83050 [Q9NX70-1] |
PTM databases
GlyGeni | Q9NX70, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9NX70 |
PhosphoSitePlusi | Q9NX70 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000063322, Expressed in prefrontal cortex and 216 other tissues |
ExpressionAtlasi | Q9NX70, baseline and differential |
Genevisiblei | Q9NX70, HS |
Organism-specific databases
HPAi | ENSG00000063322, Low tissue specificity |
Interactioni
Subunit structurei
Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Associates with the MED18/MED20 heteromer.
4 PublicationsBinary interactionsi
Q9NX70
Protein-protein interaction databases
BioGRIDi | 120734, 97 interactors |
ComplexPortali | CPX-3227, Core mediator complex |
CORUMi | Q9NX70 |
IntActi | Q9NX70, 103 interactors |
MINTi | Q9NX70 |
STRINGi | 9606.ENSP00000314343 |
Miscellaneous databases
RNActi | Q9NX70, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 48 | DisorderedSequence analysisAdd BLAST | 48 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 26 | Polar residuesSequence analysisAdd BLAST | 26 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QRNJ, Eukaryota |
GeneTreei | ENSGT00390000007540 |
HOGENOMi | CLU_1312718_0_0_1 |
InParanoidi | Q9NX70 |
OrthoDBi | 1362864at2759 |
PhylomeDBi | Q9NX70 |
TreeFami | TF326632 |
Family and domain databases
InterProi | View protein in InterPro IPR021018, Mediator_Med29_met |
PANTHERi | PTHR28314, PTHR28314, 1 hit |
Pfami | View protein in Pfam PF11568, Med29, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAASQQQASA ASSAAGVSGP SSAGGPGPQQ QPQPPAQLVG PAQSGLLQQQ
60 70 80 90 100
QQDFDPVQRY KMLIPQLKES LQTLMKVAAQ NLIQNTNIDN GQKSSDGPIQ
110 120 130 140 150
RFDKCLEEFY ALCDQLELCL RLAHECLSQS CDSAKHSPTL VPTATKPDAV
160 170 180 190 200
QPDSLPYPQY LAVIKAQISC AKDIHTALLD CANKVTGKTP APPAGPGGTL
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB4DUA7 | B4DUA7_HUMAN | Intersex-like protein | MED29 IXL, hCG_43365 | 221 | Annotation score: | ||
M0R0U2 | M0R0U2_HUMAN | Intersex-like protein | MED29 | 114 | Annotation score: | ||
M0R3F3 | M0R3F3_HUMAN | Intersex-like protein | MED29 | 208 | Annotation score: | ||
M0R0A0 | M0R0A0_HUMAN | Intersex-like protein | MED29 | 75 | Annotation score: |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056133 | 121 – 196 | RLAHE…PPAGP → PPSPTQCSLTASPTHSTWRS SKPRFPVPRTFTPPCWTVPT RSRARHPHHLLALGALCEVG DREWGRQWLVGGVQRE in isoform 2. 1 PublicationAdd BLAST | 76 | |
Alternative sequenceiVSP_056134 | 197 – 200 | Missing in isoform 2. 1 Publication | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY729650 mRNA Translation: AAU43732.1 Different initiation. AK000411 mRNA Translation: BAA91147.1 AK298014 mRNA Translation: BAG60318.1 AL137304 mRNA Translation: CAB70687.2 AC005239 Genomic DNA No translation available. AC011500 Genomic DNA No translation available. AC104106 Genomic DNA No translation available. BC019015 mRNA Translation: AAH19015.1 |
CCDSi | CCDS33021.1 [Q9NX70-1] |
PIRi | T46497 |
RefSeqi | NP_001304699.1, NM_001317770.1 [Q9NX70-2] NP_060062.1, NM_017592.2 [Q9NX70-1] |
Genome annotation databases
Ensembli | ENST00000315588; ENSP00000314343; ENSG00000063322 ENST00000594368; ENSP00000472501; ENSG00000063322 [Q9NX70-2] |
GeneIDi | 55588 |
KEGGi | hsa:55588 |
MANE-Selecti | ENST00000315588.11; ENSP00000314343.5; NM_017592.4; NP_060062.2 |
UCSCi | uc060ykk.1, human [Q9NX70-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY729650 mRNA Translation: AAU43732.1 Different initiation. AK000411 mRNA Translation: BAA91147.1 AK298014 mRNA Translation: BAG60318.1 AL137304 mRNA Translation: CAB70687.2 AC005239 Genomic DNA No translation available. AC011500 Genomic DNA No translation available. AC104106 Genomic DNA No translation available. BC019015 mRNA Translation: AAH19015.1 |
CCDSi | CCDS33021.1 [Q9NX70-1] |
PIRi | T46497 |
RefSeqi | NP_001304699.1, NM_001317770.1 [Q9NX70-2] NP_060062.1, NM_017592.2 [Q9NX70-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
7EMF | electron microscopy | 3.50 | 2 | 1-200 | [»] | |
7ENA | electron microscopy | 4.07 | b | 1-200 | [»] | |
7ENC | electron microscopy | 4.13 | b | 1-200 | [»] | |
7ENJ | electron microscopy | 4.40 | 2 | 1-200 | [»] | |
7LBM | electron microscopy | 4.80 | p | 1-200 | [»] | |
SMRi | Q9NX70 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 120734, 97 interactors |
ComplexPortali | CPX-3227, Core mediator complex |
CORUMi | Q9NX70 |
IntActi | Q9NX70, 103 interactors |
MINTi | Q9NX70 |
STRINGi | 9606.ENSP00000314343 |
PTM databases
GlyGeni | Q9NX70, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9NX70 |
PhosphoSitePlusi | Q9NX70 |
Genetic variation databases
BioMutai | MED29 |
DMDMi | 74734688 |
Proteomic databases
EPDi | Q9NX70 |
jPOSTi | Q9NX70 |
MassIVEi | Q9NX70 |
MaxQBi | Q9NX70 |
PaxDbi | Q9NX70 |
PeptideAtlasi | Q9NX70 |
PRIDEi | Q9NX70 |
ProteomicsDBi | 83050 [Q9NX70-1] |
Protocols and materials databases
Antibodypediai | 30311, 63 antibodies from 19 providers |
DNASUi | 55588 |
Genome annotation databases
Ensembli | ENST00000315588; ENSP00000314343; ENSG00000063322 ENST00000594368; ENSP00000472501; ENSG00000063322 [Q9NX70-2] |
GeneIDi | 55588 |
KEGGi | hsa:55588 |
MANE-Selecti | ENST00000315588.11; ENSP00000314343.5; NM_017592.4; NP_060062.2 |
UCSCi | uc060ykk.1, human [Q9NX70-1] |
Organism-specific databases
CTDi | 55588 |
DisGeNETi | 55588 |
GeneCardsi | MED29 |
HGNCi | HGNC:23074, MED29 |
HPAi | ENSG00000063322, Low tissue specificity |
MIMi | 612914, gene |
neXtProti | NX_Q9NX70 |
OpenTargetsi | ENSG00000063322 |
PharmGKBi | PA162395655 |
VEuPathDBi | HostDB:ENSG00000063322 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QRNJ, Eukaryota |
GeneTreei | ENSGT00390000007540 |
HOGENOMi | CLU_1312718_0_0_1 |
InParanoidi | Q9NX70 |
OrthoDBi | 1362864at2759 |
PhylomeDBi | Q9NX70 |
TreeFami | TF326632 |
Enzyme and pathway databases
PathwayCommonsi | Q9NX70 |
Reactomei | R-HSA-1989781, PPARA activates gene expression R-HSA-381340, Transcriptional regulation of white adipocyte differentiation |
SignaLinki | Q9NX70 |
SIGNORi | Q9NX70 |
Miscellaneous databases
BioGRID-ORCSi | 55588, 410 hits in 1058 CRISPR screens |
ChiTaRSi | MED29, human |
GenomeRNAii | 55588 |
Pharosi | Q9NX70, Tbio |
PROi | PR:Q9NX70 |
RNActi | Q9NX70, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000063322, Expressed in prefrontal cortex and 216 other tissues |
ExpressionAtlasi | Q9NX70, baseline and differential |
Genevisiblei | Q9NX70, HS |
Family and domain databases
InterProi | View protein in InterPro IPR021018, Mediator_Med29_met |
PANTHERi | PTHR28314, PTHR28314, 1 hit |
Pfami | View protein in Pfam PF11568, Med29, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MED29_HUMAN | |
Accessioni | Q9NX70Primary (citable) accession number: Q9NX70 Secondary accession number(s): B4DNQ6 Q9NTF4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 29, 2007 |
Last sequence update: | October 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families