THG1L

Functionⁱ

Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis.1 Publication

Catalytic activityⁱ

p-tRNA(His) + ATP + GTP = pppG-P-tRNA(His) + AMP + diphosphate.

Cofactorⁱ

Mg²⁺1 PublicationNote: Binds 2 magnesium ions per subunit.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	58	Magnesium 1; catalytic1 Publication	1
Metal bindingⁱ	58	Magnesium 2; catalytic1 Publication	1
Metal bindingⁱ	59	Magnesium 1; via carbonyl oxygen; catalytic1 Publication	1
Metal bindingⁱ	105	Magnesium 1; catalytic1 Publication	1
Metal bindingⁱ	105	Magnesium 2; catalytic1 Publication	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	58 – 63	GTP1 Publication		6
Nucleotide bindingⁱ	104 – 105	GTP1 Publication		2

GO - Molecular functionⁱ

ATP binding
Source: BHF-UCL
Inferred from direct assayⁱ
- 21059936
GTP binding
Source: BHF-UCL
Inferred from direct assayⁱ
- 21059936
identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
magnesium ion binding
Source: BHF-UCL
Inferred from direct assayⁱ
- 21059936
nucleotidyltransferase activity
Source: Reactome
Inferred from experimentⁱ
- 21059936
tRNA binding
Source: BHF-UCL
Traceable author statementⁱ
- 21059936
tRNA guanylyltransferase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 21059936

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

protein homotetramerization
Source: BHF-UCL
Inferred from direct assayⁱ
- 21059936
tRNA modification Source: UniProtKB
tRNA processing
Source: UniProtKB
Inferred from direct assayⁱ
- 21059936

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Nucleotidyltransferase, Transferase
Biological process	tRNA processing
Ligand	GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAⁱ	2.7.7.79 2681
Reactomeⁱ	R-HSA-6782315 tRNA modification in the nucleus and cytosol

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Probable tRNA(His) guanylyltransferase (EC:2.7.7.79) Alternative name(s): Interphase cytoplasmic foci protein 45 tRNA-histidine guanylyltransferase
Gene namesⁱ	Name:THG1L Synonyms:ICF45
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 5

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000113272.13
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:26053 THG1L
neXtProtⁱ	NX_Q9NWX6

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	58	D → A: Reduces activity by 99.5%. 1 Publication	1
Mutagenesisⁱ	63	H → A: Slightly reduced enzyme activity. 1 Publication	1
Mutagenesisⁱ	104	S → A: Slightly reduced enzyme activity. 1 Publication	1
Mutagenesisⁱ	105	D → A: Loss of enzyme activity. 1 Publication	1
Mutagenesisⁱ	106	E → A: Reduces activity by 95%. 1 Publication	1
Mutagenesisⁱ	127	T → A: Abolishes oligomerization. Loss of enzyme activity. 1 Publication	1
Mutagenesisⁱ	181	H → A: Loss of enzyme activity. 1 Publication	1
Mutagenesisⁱ	216	K → A: Reduces activity by 98.5%. 1 Publication	1
Mutagenesisⁱ	227	N → A: Loss of enzyme activity. 1 Publication	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	54974
Open Targets More...OpenTargetsⁱ	ENSG00000113272
PharmGKBⁱ	PA162405691

Polymorphism and mutation databases

BioMutaⁱ	THG1L
DMDMⁱ	146325755

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000284984	1 – 298	Probable tRNA(His) guanylyltransferaseAdd BLAST		298

Proteomic databases

EPDⁱ	Q9NWX6
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9NWX6
PaxDbⁱ	Q9NWX6
PeptideAtlas More...PeptideAtlasⁱ	Q9NWX6
PRIDEⁱ	Q9NWX6
ProteomicsDBⁱ	82998

PTM databases

iPTMnetⁱ	Q9NWX6
PhosphoSitePlusⁱ	Q9NWX6

Expressionⁱ

Tissue specificityⁱ

Expressed in many tissues.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000113272 Expressed in 182 organ(s), highest expression level in testis
CleanExⁱ	HS_THG1L
ExpressionAtlasⁱ	Q9NWX6 baseline and differential
Genevisibleⁱ	Q9NWX6 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA035877 HPA035878

HPAⁱ

HPA035877
HPA035878

Interactionⁱ

Subunit structureⁱ

Homotetramer.1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
itself		4	EBI-746510,EBI-746510
SDCBP	O00560	3	EBI-746510,EBI-727004
TERF1	P54274	2	EBI-746510,EBI-710997

GO - Molecular functionⁱ

identical protein binding
Source: IntAct
Inferred from physical interactionⁱ

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	120311, 30 interactors
Database of interacting proteins More...DIPⁱ	DIP-59479N
IntActⁱ	Q9NWX6, 11 interactors
Molecular INTeraction database More...MINTⁱ	Q9NWX6
STRINGⁱ	9606.ENSP00000231198

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	36 – 41	Combined sources	6
Beta strandⁱ	51 – 59	Combined sources	9
Helixⁱ	62 – 68	Combined sources	7
Helixⁱ	77 – 93	Combined sources	17
Beta strandⁱ	94 – 103	Combined sources	10
Beta strandⁱ	106 – 111	Combined sources	6
Turnⁱ	117 – 120	Combined sources	4
Helixⁱ	122 – 139	Combined sources	18
Helixⁱ	141 – 144	Combined sources	4
Beta strandⁱ	146 – 148	Combined sources	3
Beta strandⁱ	156 – 165	Combined sources	10
Helixⁱ	166 – 195	Combined sources	30
Helixⁱ	201 – 208	Combined sources	8
Helixⁱ	213 – 224	Combined sources	12
Helixⁱ	228 – 230	Combined sources	3
Helixⁱ	233 – 237	Combined sources	5
Beta strandⁱ	239 – 243	Combined sources	5
Beta strandⁱ	274 – 277	Combined sources	4
Beta strandⁱ	281 – 283	Combined sources	3
Helixⁱ	284 – 289	Combined sources	6
Helixⁱ	292 – 295	Combined sources	4

Show more details

3D structure databases

ProteinModelPortalⁱ	Q9NWX6
SMRⁱ	Q9NWX6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q9NWX6

EvolutionaryTraceⁱ

Q9NWX6

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the tRNA(His) guanylyltransferase family.Curated

Phylogenomic databases

eggNOGⁱ	KOG2721 Eukaryota COG4021 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000011705
HOGENOMⁱ	HOG000204890
HOVERGENⁱ	HBG054289
InParanoidⁱ	Q9NWX6
KEGG Orthology (KO) More...KOⁱ	K10761
OMAⁱ	NTAFWKL
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0GYS
PhylomeDBⁱ	Q9NWX6
TreeFamⁱ	TF325119

Family and domain databases

Gene3Dⁱ	3.30.70.3000, 1 hit
InterProⁱ	View protein in InterPro IPR025845 Thg1_C_dom IPR024956 tRNAHis_GuaTrfase_cat IPR007537 tRNAHis_GuaTrfase_Thg1 IPR038469 tRNAHis_GuaTrfase_Thg1_sf
PANTHERⁱ	PTHR12729 PTHR12729, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF04446 Thg1, 1 hit PF14413 Thg1C, 1 hit
PIRSFⁱ	PIRSF028980 tRNAHis_guanylyltransferase, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.ⁱShow all

Q9NWX6-1 [UniParc]FASTA Add to basket

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        10         20         30         40         50
MWGACKVKVH DSLATISITL RRYLRLGATM AKSKFEYVRD FEADDTCLAH 
        60         70         80         90        100
CWVVVRLDGR NFHRFAEKHN FAKPNDSRAL QLMTKCAQTV MEELEDIVIA 
       110        120        130        140        150
YGQSDEYSFV FKRKTNWFKR RASKFMTHVA SQFASSYVFY WRDYFEDQPL 
       160        170        180        190        200
LYPPGFDGRV VVYPSNQTLK DYLSWRQADC HINNLYNTVF WALIQQSGLT 
       210        220        230        240        250
PVQAQGRLQG TLAADKNEIL FSEFNINYNN ELPMYRKGTV LIWQKVDEVM 
       260        270        280        290 
TKEIKLPTEM EGKKMAVTRT RTKPVPLHCD IIGDAFWKEH PEILDEDS

Length:298

Mass (Da):34,831

Last modified:May 1, 2007 - v2

Checksum:ⁱA6C941B75611C448

GO

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation
	E5RIQ8	E5RIQ8_HUMAN		Probable tRNA(His) guanylyltransfer... Probable tRNA(His) guanylyltransferase	THG1L	124	Annotation score:
	H0YC78	H0YC78_HUMAN		Probable tRNA(His) guanylyltransfer... Probable tRNA(His) guanylyltransferase	THG1L	34	Annotation score:

Sequence cautionⁱ

The sequence AAH01523 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

The sequence AAH01852 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	57	L → Q in BAD96839 (Ref. 5) Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_031871	232	L → P4 PublicationsCorresponds to variant dbSNP:rs2270812Ensembl.		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY463216 mRNA Translation: AAS21134.1 AL136669 mRNA Translation: CAB66604.1 AK000553 mRNA Translation: BAA91249.1 AK021663 mRNA Translation: BAB13870.1 CR533503 mRNA Translation: CAG38534.1 AK223119 mRNA Translation: BAD96839.1 CH471062 Genomic DNA Translation: EAW61590.1 CH471062 Genomic DNA Translation: EAW61591.1 BC001523 mRNA Translation: AAH01523.2 Different initiation. BC001852 mRNA Translation: AAH01852.2 Different initiation. BC023521 mRNA Translation: AAH23521.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS4341.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001304753.1, NM_001317824.1 NP_001304754.1, NM_001317825.1 NP_001304755.1, NM_001317826.1 NP_060342.2, NM_017872.4
UniGeneⁱ	Hs.353090 Hs.734786

Genome annotation databases

Ensemblⁱ	ENST00000231198; ENSP00000231198; ENSG00000113272
GeneIDⁱ	54974
KEGGⁱ	hsa:54974
UCSC genome browser More...UCSCⁱ	uc003lxd.4 human

Keywords - Coding sequence diversityⁱ

Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9NWX6	cDNA FLJ13293 fis, clone OVARC1001188		HUMAN		173	UniRef100_Q9NWX6
UPI00001BD8F2		HUMAN		269

Protein	Similar proteins		Species	Score	Length	Source
Q9NWX6	Probable tRNA(His) guanylyltransferase		BOVIN		298	UniRef90_Q9NWX6
cDNA FLJ13293 fis, clone OVARC1001188		HUMAN		173
tRNA(His) guanylyltransferase		ODORO		298
tRNA(His) guanylyltransferase		PIG		298
tRNA(His) guanylyltransferase		AOTNA		298
+76

Protein	Similar proteins		Species	Score	Length	Source
Q9NWX6	Probable tRNA(His) guanylyltransferase		BOVIN		298	UniRef50_Q9NWX6
cDNA FLJ13293 fis, clone OVARC1001188		HUMAN		173
tRNA(His) guanylyltransferase		TARSY		298
tRNA(His) guanylyltransferase		PIG		298
tRNA(His) guanylyltransferase		PAPAN		298
+156

Entry informationⁱ

Entry nameⁱ	THG1_HUMAN
Accessionⁱ	Q9NWX6Primary (citable) accession number: Q9NWX6 Secondary accession number(s): D3DQJ5 , Q53G12, Q7L5R3, Q9H0S2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
	Last sequence update:	May 1, 2007
	Last modified:	September 12, 2018
	This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9NWX6 (THG1_HUMAN)

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Probable tRNA(His) guanylyltransferase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Cytosol

Mitochondrion

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Computationally mapped potential isoform sequencesi

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Computationally mapped potential isoform sequencesⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ