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Entry version 190 (25 May 2022)
Sequence version 2 (16 Jun 2003)
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Protein

Hypoxia-inducible factor 1-alpha inhibitor

Gene

HIF1AN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.

8 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Fe2+8 Publications

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

The kinetic constants are determined for the recombinant FLAG-His-tagged protein.
  1. KM=100 µM for HIF1A (788-822) peptide1 Publication
  2. KM=160 µM for HIF2A (832-866) peptide1 Publication
  3. KM=0.5 µM for Fe2+1 Publication
  4. KM=25 µM for 2-oxoglutarate1 Publication
  5. KM=260 µM for ascorbate1 Publication
  6. KM=90 µM for O21 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei1452-oxoglutarate3 Publications1
Binding sitei152Substrate1 Publication1
Binding sitei1962-oxoglutarate3 Publications1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi199Iron; via tele nitrogen; catalytic8 Publications1
Metal bindingi201Iron; catalytic8 Publications1
Binding sitei2052-oxoglutarate3 Publications1
Binding sitei2142-oxoglutarate3 Publications1
Metal bindingi279Iron; via tele nitrogen; catalytic8 Publications1
Binding sitei2942-oxoglutarate3 Publications1
Binding sitei300Substrate; via amide nitrogen1 Publication1
Binding sitei321Substrate1 Publication1
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei340Important for dimer formation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processTranscription, Transcription regulation
LigandIron, Metal-binding

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
1.14.11.16, 2681
1.14.11.30, 2681

Pathway Commons web resource for biological pathway data

More...
PathwayCommonsi
Q9NWT6

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-HSA-1234174, Cellular response to hypoxia

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...
SignaLinki
Q9NWT6

SIGNOR Signaling Network Open Resource

More...
SIGNORi
Q9NWT6

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Hypoxia-inducible factor 1-alpha inhibitor (EC:1.14.11.30, EC:1.14.11.n4)
Alternative name(s):
Factor inhibiting HIF-1
Short name:
FIH-1
Hypoxia-inducible factor asparagine hydroxylase
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:HIF1AN
Synonyms:FIH1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 10

Organism-specific databases

Human Gene Nomenclature Database

More...
HGNCi
HGNC:17113, HIF1AN

Online Mendelian Inheritance in Man (OMIM)

More...
MIMi
606615, gene

neXtProt; the human protein knowledge platform

More...
neXtProti
NX_Q9NWT6

Eukaryotic Pathogen, Vector and Host Database Resources

More...
VEuPathDBi
HostDB:ENSG00000166135

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi199H → A: Prevents suppression of HIF CAD activity. Strongly stimulates 2-oxoglutarate turnover. No stimulation of 2-oxoglutarate turnover; when associated with R-340. 1 Publication1
Mutagenesisi201D → A: Prevents suppression of HIF CAD activity. 3 Publications1
Mutagenesisi201D → E: Loss of HIF1A Asn hydroxylation activity. Slightly stimulates 2-oxoglutarate turnover. 3 Publications1
Mutagenesisi201D → G: No impact on HIF1A Asn hydroxylation activity. Loss of Asp hydroxylation ability. Strongly stimulates 2-oxoglutarate turnover. Loss of HIF1A Asn hydroxylation activity and slight stimulation of 2-oxoglutarate turnover; when associated with R-296. 3 Publications1
Mutagenesisi239Q → H: No effect on Asp hydroxylation ability. 1 Publication1
Mutagenesisi296W → R: Loss of HIF1A Asn hydroxylation activity and slight stimulation of 2-oxoglutarate turnover; when associated with G-201. 1 Publication1
Mutagenesisi340L → R: Impairs dimer formation, leading to loss of HIF1A Asn hydroxylation activity. No stimulation of 2-oxoglutarate turnover; when associated with A-201. 2 Publications1
Mutagenesisi344I → R: No effect on dimer formation and HIF1A Asn hydroxylation activity. 1 Publication1

Organism-specific databases

DisGeNET

More...
DisGeNETi
55662

Open Targets

More...
OpenTargetsi
ENSG00000166135

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...
PharmGKBi
PA29284

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...
Pharosi
Q9NWT6, Tbio

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...
ChEMBLi
CHEMBL5909

Drug and drug target database

More...
DrugBanki
DB01694, D-tartaric acid
DB08263, N-(carboxycarbonyl)-D-phenylalanine

DrugCentral

More...
DrugCentrali
Q9NWT6

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...
BioMutai
HIF1AN

Domain mapping of disease mutations (DMDM)

More...
DMDMi
32129605

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemovedCombined sources
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000839742 – 349Hypoxia-inducible factor 1-alpha inhibitorAdd BLAST348

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
Q9NWT6

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
Q9NWT6

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...
MassIVEi
Q9NWT6

MaxQB - The MaxQuant DataBase

More...
MaxQBi
Q9NWT6

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q9NWT6

PeptideAtlas

More...
PeptideAtlasi
Q9NWT6

PRoteomics IDEntifications database

More...
PRIDEi
Q9NWT6

ProteomicsDB: a multi-organism proteome resource

More...
ProteomicsDBi
82978

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q9NWT6

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
Q9NWT6

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSG00000166135, Expressed in skeletal muscle tissue and 233 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
Q9NWT6, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
Q9NWT6, HS

Organism-specific databases

Human Protein Atlas

More...
HPAi
ENSG00000166135, Tissue enhanced (skeletal muscle, tongue)

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer; homodimerization is essential for catalytic activity.

Interacts with VHL and HIF1A.

Part of a complex with VHL, HIF1A and HDAC1 or HDAC2 or HDAC3.

Interacts with NFKB1 and NFKBIA.

Interacts with NOTCH1, NOTCH2 and NOTCH3 but not with NOTCH4.

Interacts with APBA3; binding inhibits HIF1AN binding to HIF1A.

Interacts with TNKS2.

Interacts with PPP1R12A.

Interacts with ASB4 (By similarity).

Interacts with UBE3A.

Interacts with ANKS3 (By similarity).

Interacts with NECAB3; the interaction is indirect and seems to be mediated by APBA3.

By similarity18 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
120794, 313 interactors

Database of interacting proteins

More...
DIPi
DIP-35527N

Protein interaction database and analysis system

More...
IntActi
Q9NWT6, 213 interactors

Molecular INTeraction database

More...
MINTi
Q9NWT6

STRING: functional protein association networks

More...
STRINGi
9606.ENSP00000299163

Chemistry databases

BindingDB database of measured binding affinities

More...
BindingDBi
Q9NWT6

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
Q9NWT6, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1349
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

AlphaFold Protein Structure Database

More...
AlphaFoldDBi
Q9NWT6

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q9NWT6

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
Q9NWT6

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini142 – 312JmjCPROSITE-ProRule annotationAdd BLAST171

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 51DisorderedSequence analysisAdd BLAST51
Regioni2 – 125Interaction with VHL1 PublicationAdd BLAST124
Regioni181 – 183Substrate binding1 Publication3
Regioni201 – 203Substrate binding1 Publication3
Regioni238 – 239Substrate binding1 Publication2

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG2132, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000157409

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_016785_3_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q9NWT6

Identification of Orthologs from Complete Genome Data

More...
OMAi
CLYPHPV

Database of Orthologous Groups

More...
OrthoDBi
1385616at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q9NWT6

TreeFam database of animal gene trees

More...
TreeFami
TF329609

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.287.1010, 1 hit
2.60.120.10, 1 hit

Intrinsically Disordered proteins with Extensive Annotations and Literature

More...
IDEALi
IID00396

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR041667, Cupin_8
IPR027445, FIH-1
IPR027452, FIH-1_dom_II
IPR003347, JmjC_dom
IPR014710, RmlC-like_jellyroll

The PANTHER Classification System

More...
PANTHERi
PTHR12461:SF80, PTHR12461:SF80, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF13621, Cupin_8, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00558, JmjC, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51184, JMJC, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q9NWT6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAATAAEAVA SGSGEPREEA GALGPAWDES QLRSYSFPTR PIPRLSQSDP
60 70 80 90 100
RAEELIENEE PVVLTDTNLV YPALKWDLEY LQENIGNGDF SVYSASTHKF
110 120 130 140 150
LYYDEKKMAN FQNFKPRSNR EEMKFHEFVE KLQDIQQRGG EERLYLQQTL
160 170 180 190 200
NDTVGRKIVM DFLGFNWNWI NKQQGKRGWG QLTSNLLLIG MEGNVTPAHY
210 220 230 240 250
DEQQNFFAQI KGYKRCILFP PDQFECLYPY PVHHPCDRQS QVDFDNPDYE
260 270 280 290 300
RFPNFQNVVG YETVVGPGDV LYIPMYWWHH IESLLNGGIT ITVNFWYKGA
310 320 330 340
PTPKRIEYPL KAHQKVAIMR NIEKMLGEAL GNPQEVGPLL NTMIKGRYN
Length:349
Mass (Da):40,285
Last modified:June 16, 2003 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i96A033BA7B3BD8C7
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PNR8E9PNR8_HUMAN
Hypoxia-inducible factor 1-alpha in...
HIF1AN
35Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E9PL41E9PL41_HUMAN
Hypoxia-inducible factor 1-alpha in...
HIF1AN
191Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti10A → T in BAA91291 (PubMed:14702039).Curated1
Sequence conflicti28D → H in BAA91291 (PubMed:14702039).Curated1
Sequence conflicti156R → G in BAA91291 (PubMed:14702039).Curated1

<p>This subsection of the 'Sequence' section reports information derived from mass spectrometry experiments done on the entire protein or on biologically active derived peptide(s).<p><a href='/help/mass_spectrometry' target='_top'>More...</a></p>Mass spectrometryi

Molecular mass is 40566 Da. Determined by ESI. 1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_05102841P → A1 PublicationCorresponds to variant dbSNP:rs2295778Ensembl.1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
AF395830 mRNA Translation: AAL27308.1
AK000622 mRNA Translation: BAA91291.1
AL133352 Genomic DNA No translation available.
CH471066 Genomic DNA Translation: EAW49817.1
CH471066 Genomic DNA Translation: EAW49818.1
BC007719 mRNA Translation: AAH07719.1
AL359615 mRNA Translation: CAB94885.1

The Consensus CDS (CCDS) project

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CCDSi
CCDS7498.1

Protein sequence database of the Protein Information Resource

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PIRi
T50633

NCBI Reference Sequences

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RefSeqi
NP_060372.2, NM_017902.2

Genome annotation databases

Ensembl eukaryotic genome annotation project

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Ensembli
ENST00000299163.7; ENSP00000299163.4; ENSG00000166135.14

Database of genes from NCBI RefSeq genomes

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GeneIDi
55662

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
hsa:55662

Matched Annotation from NCBI and EMBL-EBI (MANE) - Phase one

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MANE-Selecti
ENST00000299163.7; ENSP00000299163.4; NM_017902.3; NP_060372.2

UCSC genome browser

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UCSCi
uc001krj.5, human

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF395830 mRNA Translation: AAL27308.1
AK000622 mRNA Translation: BAA91291.1
AL133352 Genomic DNA No translation available.
CH471066 Genomic DNA Translation: EAW49817.1
CH471066 Genomic DNA Translation: EAW49818.1
BC007719 mRNA Translation: AAH07719.1
AL359615 mRNA Translation: CAB94885.1
CCDSiCCDS7498.1
PIRiT50633
RefSeqiNP_060372.2, NM_017902.2

3D structure databases

Select the link destinations:

Protein Data Bank Europe

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PDBei

Protein Data Bank RCSB

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RCSB PDBi

Protein Data Bank Japan

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PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H2KX-ray2.15A1-349[»]
1H2LX-ray2.25A1-349[»]
1H2MX-ray2.50A1-349[»]
1H2NX-ray2.84A1-349[»]
1IZ3X-ray2.80A1-349[»]
1MZEX-ray2.20A1-349[»]
1MZFX-ray2.40A1-349[»]
1YCIX-ray2.70A1-349[»]
2CGNX-ray2.40A1-349[»]
2CGOX-ray2.30A1-349[»]
2ILMX-ray2.30A1-349[»]
2W0XX-ray2.12A1-349[»]
2WA3X-ray2.50A1-349[»]
2WA4X-ray2.50A1-349[»]
2XUMX-ray2.20A1-349[»]
2Y0IX-ray2.28A1-349[»]
2YC0X-ray2.15A1-349[»]
2YDEX-ray2.28A1-349[»]
3D8CX-ray2.10A11-349[»]
3KCXX-ray2.60A15-349[»]
3KCYX-ray2.59A15-349[»]
3OD4X-ray2.20A1-349[»]
3P3NX-ray2.40A1-349[»]
3P3PX-ray2.60A1-349[»]
4AI8X-ray2.40A1-349[»]
4B7EX-ray2.50A1-349[»]
4B7KX-ray2.39A1-349[»]
4BIOX-ray2.45A1-349[»]
4JAAX-ray2.39A1-349[»]
4NR1X-ray2.68A1-349[»]
4Z1VX-ray2.10A1-349[»]
4Z2WX-ray2.50A1-349[»]
5JWKX-ray2.30A1-349[»]
5JWLX-ray2.40A1-349[»]
5JWPX-ray2.10A1-349[»]
5OP6X-ray2.45A1-349[»]
5OP8X-ray2.30A1-349[»]
5OPCX-ray2.30A1-349[»]
6H9JX-ray1.83A12-349[»]
6HA6X-ray1.98A1-349[»]
6HC8X-ray1.90A1-349[»]
6HKPX-ray1.90A1-349[»]
6HL5X-ray1.98A1-349[»]
6HL6X-ray1.97A1-349[»]
6RUJX-ray2.42A1-349[»]
7A1JX-ray1.90A1-349[»]
7A1KX-ray1.99A1-349[»]
7A1LX-ray2.29A1-349[»]
7A1MX-ray2.18A1-349[»]
7A1NX-ray2.01A1-349[»]
7A1OX-ray2.21A1-349[»]
7A1PX-ray1.76A1-349[»]
7A1QX-ray1.75A1-349[»]
7A1SX-ray2.01A1-349[»]
AlphaFoldDBiQ9NWT6
SMRiQ9NWT6
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi120794, 313 interactors
DIPiDIP-35527N
IntActiQ9NWT6, 213 interactors
MINTiQ9NWT6
STRINGi9606.ENSP00000299163

Chemistry databases

BindingDBiQ9NWT6
ChEMBLiCHEMBL5909
DrugBankiDB01694, D-tartaric acid
DB08263, N-(carboxycarbonyl)-D-phenylalanine
DrugCentraliQ9NWT6

PTM databases

iPTMnetiQ9NWT6
PhosphoSitePlusiQ9NWT6

Genetic variation databases

BioMutaiHIF1AN
DMDMi32129605

Proteomic databases

EPDiQ9NWT6
jPOSTiQ9NWT6
MassIVEiQ9NWT6
MaxQBiQ9NWT6
PaxDbiQ9NWT6
PeptideAtlasiQ9NWT6
PRIDEiQ9NWT6
ProteomicsDBi82978

Protocols and materials databases

Antibodypedia a portal for validated antibodies

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Antibodypediai
17685, 655 antibodies from 36 providers

The DNASU plasmid repository

More...
DNASUi
55662

Genome annotation databases

EnsembliENST00000299163.7; ENSP00000299163.4; ENSG00000166135.14
GeneIDi55662
KEGGihsa:55662
MANE-SelectiENST00000299163.7; ENSP00000299163.4; NM_017902.3; NP_060372.2
UCSCiuc001krj.5, human

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
55662
DisGeNETi55662

GeneCards: human genes, protein and diseases

More...
GeneCardsi
HIF1AN
HGNCiHGNC:17113, HIF1AN
HPAiENSG00000166135, Tissue enhanced (skeletal muscle, tongue)
MIMi606615, gene
neXtProtiNX_Q9NWT6
OpenTargetsiENSG00000166135
PharmGKBiPA29284
VEuPathDBiHostDB:ENSG00000166135

GenAtlas: human gene database

More...
GenAtlasi
Search...

Phylogenomic databases

eggNOGiKOG2132, Eukaryota
GeneTreeiENSGT00940000157409
HOGENOMiCLU_016785_3_0_1
InParanoidiQ9NWT6
OMAiCLYPHPV
OrthoDBi1385616at2759
PhylomeDBiQ9NWT6
TreeFamiTF329609

Enzyme and pathway databases

BRENDAi1.14.11.16, 2681
1.14.11.30, 2681
PathwayCommonsiQ9NWT6
ReactomeiR-HSA-1234174, Cellular response to hypoxia
SignaLinkiQ9NWT6
SIGNORiQ9NWT6

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...
BioGRID-ORCSi
55662, 32 hits in 1091 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
HIF1AN, human
EvolutionaryTraceiQ9NWT6

The Gene Wiki collection of pages on human genes and proteins

More...
GeneWikii
HIF1AN

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
55662
PharosiQ9NWT6, Tbio

Protein Ontology

More...
PROi
PR:Q9NWT6
RNActiQ9NWT6, protein

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSG00000166135, Expressed in skeletal muscle tissue and 233 other tissues
ExpressionAtlasiQ9NWT6, baseline and differential
GenevisibleiQ9NWT6, HS

Family and domain databases

Gene3Di1.10.287.1010, 1 hit
2.60.120.10, 1 hit
IDEALiIID00396
InterProiView protein in InterPro
IPR041667, Cupin_8
IPR027445, FIH-1
IPR027452, FIH-1_dom_II
IPR003347, JmjC_dom
IPR014710, RmlC-like_jellyroll
PANTHERiPTHR12461:SF80, PTHR12461:SF80, 1 hit
PfamiView protein in Pfam
PF13621, Cupin_8, 1 hit
SMARTiView protein in SMART
SM00558, JmjC, 1 hit
PROSITEiView protein in PROSITE
PS51184, JMJC, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiHIF1N_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9NWT6
Secondary accession number(s): D3DR69
, Q5W147, Q969Q7, Q9NPV5
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: May 25, 2022
This is version 190 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with genetic variants
    List of human entries with genetic variants
  3. Human variants curated from literature reports
    Index of human variants curated from literature reports
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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