UniProtKB - Q9NWS9 (ZN446_HUMAN)
Protein
Zinc finger protein 446
Gene
ZNF446
Organism
Homo sapiens (Human)
Status
Functioni
May be involved in transcriptional regulation.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 332 – 359 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 28 | |
Zinc fingeri | 395 – 422 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 28 | |
Zinc fingeri | 423 – 450 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 28 |
GO - Molecular functioni
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
- identical protein binding Source: IntAct
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9NWS9 |
Reactomei | R-HSA-212436, Generic Transcription Pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger protein 446Alternative name(s): Zinc finger protein with KRAB and SCAN domains 20 |
Gene namesi | Name:ZNF446 Synonyms:ZKSCAN20 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000083838.15 |
HGNCi | HGNC:21036, ZNF446 |
neXtProti | NX_Q9NWS9 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Extracellular region or secreted
- extracellular space Source: UniProtKB
Nucleus
- nuclear chromatin Source: NTNU_SB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 55663 |
OpenTargetsi | ENSG00000083838 |
PharmGKBi | PA134863395 |
Miscellaneous databases
Pharosi | Q9NWS9, Tdark |
Polymorphism and mutation databases
BioMutai | ZNF446 |
DMDMi | 55976772 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047596 | 1 – 450 | Zinc finger protein 446Add BLAST | 450 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 130 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 137 | PhosphoserineCombined sources | 1 | |
Modified residuei | 218 | PhosphoserineCombined sources | 1 | |
Modified residuei | 308 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 330 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9NWS9 |
jPOSTi | Q9NWS9 |
MassIVEi | Q9NWS9 |
MaxQBi | Q9NWS9 |
PaxDbi | Q9NWS9 |
PeptideAtlasi | Q9NWS9 |
PRIDEi | Q9NWS9 |
ProteomicsDBi | 82976 |
PTM databases
iPTMneti | Q9NWS9 |
PhosphoSitePlusi | Q9NWS9 |
Expressioni
Gene expression databases
Bgeei | ENSG00000083838, Expressed in adenohypophysis and 158 other tissues |
ExpressionAtlasi | Q9NWS9, baseline and differential |
Genevisiblei | Q9NWS9, HS |
Organism-specific databases
HPAi | ENSG00000083838, Low tissue specificity |
Interactioni
Binary interactionsi
Q9NWS9
With | #Exp. | IntAct |
---|---|---|
PGBD1 [Q96JS3] | 5 | EBI-3925851,EBI-10290053 |
ZNF446 - isoform 2 [Q9NWS9-2]
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 120795, 71 interactors |
IntActi | Q9NWS9, 68 interactors |
STRINGi | 9606.ENSP00000472802 |
Miscellaneous databases
RNActi | Q9NWS9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 108 | SCAN boxPROSITE-ProRule annotationAdd BLAST | 83 | |
Domaini | 208 – 254 | KRABAdd BLAST | 47 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 274 – 331 | Pro-richAdd BLAST | 58 |
Sequence similaritiesi
Belongs to the krueppel C2H2-type zinc-finger protein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 332 – 359 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 28 | |
Zinc fingeri | 395 – 422 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 28 | |
Zinc fingeri | 423 – 450 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 28 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000162092 |
InParanoidi | Q9NWS9 |
OMAi | RRKPYTC |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q9NWS9 |
TreeFami | TF337489 |
Family and domain databases
CDDi | cd07765, KRAB_A-box, 1 hit cd07936, SCAN, 1 hit |
Gene3Di | 1.10.4020.10, 1 hit |
InterProi | View protein in InterPro IPR001909, KRAB IPR036051, KRAB_dom_sf IPR003309, SCAN_dom IPR038269, SCAN_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF01352, KRAB, 1 hit PF02023, SCAN, 1 hit PF00096, zf-C2H2, 1 hit |
SMARTi | View protein in SMART SM00349, KRAB, 1 hit SM00431, SCAN, 1 hit SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF109640, SSF109640, 1 hit SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS50804, SCAN_BOX, 1 hit PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9NWS9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPSPLGPPCL PVMDPETTLE EPETARLRFR GFCYQEVAGP REALARLREL
60 70 80 90 100
CCQWLQPEAH SKEQMLEMLV LEQFLGTLPP EIQAWVRGQR PGSPEEAAAL
110 120 130 140 150
VEGLQHDPGQ LLGWITAHVL KQEVLPAAQK TEEPLGSPHP SGTVESPGEG
160 170 180 190 200
PQDTRIEGSV QLSCSVKEEP NVDGQEVAPS SPPLAAQSPE GNHGHQEPAS
210 220 230 240 250
TSFHPPRIQE EWGLLDRSQK ELYWDAMLEK YGTVVSLGLP PHQPEAQAQS
260 270 280 290 300
ELGMLLTGTG VCRSLRSGNE SEGPPGCPEA QPPQGPGPAA WEGLSGAATP
310 320 330 340 350
APTVRPGTPP VPTQPTPAET RLEPAATPRK PYTCEQCGRG FDWKSVFVIH
360 370 380 390 400
HRTHTSGPGV QSPGLATGES TEKPPQGEVA FPHHPRRSLT GPRSYPCEEC
410 420 430 440 450
GCSFSWKSQL VIHRKSHTGQ RRHFCSDCGR AFDWKSQLVI HRKGHRPEVP
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketM0R202 | M0R202_HUMAN | Zinc finger protein 446 | ZNF446 | 399 | Annotation score: | ||
A0A0A0MR84 | A0A0A0MR84_HUMAN | Zinc finger protein 446 | ZNF446 | 336 | Annotation score: | ||
A0A0B4J2G2 | A0A0B4J2G2_HUMAN | Zinc finger protein 446 | ZNF446 | 422 | Annotation score: | ||
M0R034 | M0R034_HUMAN | Zinc finger protein 446 | ZNF446 | 141 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_033566 | 192 | N → H1 PublicationCorresponds to variant dbSNP:rs893185Ensembl. | 1 | |
Natural variantiVAR_033567 | 300 | P → S. Corresponds to variant dbSNP:rs36095067Ensembl. | 1 | |
Natural variantiVAR_052833 | 387 | R → H1 PublicationCorresponds to variant dbSNP:rs882610Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_058945 | 417 – 444 | Missing in isoform 2. Add BLAST | 28 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK000633 mRNA Translation: BAA91298.1 AY280800 mRNA Translation: AAQ16304.1 BC017206 mRNA Translation: AAH17206.1 |
CCDSi | CCDS12982.1 [Q9NWS9-1] |
RefSeqi | NP_060378.1, NM_017908.3 [Q9NWS9-1] XP_005259109.1, XM_005259052.4 |
Genome annotation databases
Ensembli | ENST00000594369; ENSP00000472802; ENSG00000083838 [Q9NWS9-1] ENST00000610298; ENSP00000478778; ENSG00000083838 [Q9NWS9-2] |
GeneIDi | 55663 |
KEGGi | hsa:55663 |
UCSCi | uc002qsz.4, human [Q9NWS9-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK000633 mRNA Translation: BAA91298.1 AY280800 mRNA Translation: AAQ16304.1 BC017206 mRNA Translation: AAH17206.1 |
CCDSi | CCDS12982.1 [Q9NWS9-1] |
RefSeqi | NP_060378.1, NM_017908.3 [Q9NWS9-1] XP_005259109.1, XM_005259052.4 |
3D structure databases
SMRi | Q9NWS9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 120795, 71 interactors |
IntActi | Q9NWS9, 68 interactors |
STRINGi | 9606.ENSP00000472802 |
PTM databases
iPTMneti | Q9NWS9 |
PhosphoSitePlusi | Q9NWS9 |
Polymorphism and mutation databases
BioMutai | ZNF446 |
DMDMi | 55976772 |
Proteomic databases
EPDi | Q9NWS9 |
jPOSTi | Q9NWS9 |
MassIVEi | Q9NWS9 |
MaxQBi | Q9NWS9 |
PaxDbi | Q9NWS9 |
PeptideAtlasi | Q9NWS9 |
PRIDEi | Q9NWS9 |
ProteomicsDBi | 82976 |
Protocols and materials databases
ABCDi | Q9NWS9, 6 sequenced antibodies |
Antibodypediai | 19695, 106 antibodies |
Genome annotation databases
Ensembli | ENST00000594369; ENSP00000472802; ENSG00000083838 [Q9NWS9-1] ENST00000610298; ENSP00000478778; ENSG00000083838 [Q9NWS9-2] |
GeneIDi | 55663 |
KEGGi | hsa:55663 |
UCSCi | uc002qsz.4, human [Q9NWS9-1] |
Organism-specific databases
CTDi | 55663 |
DisGeNETi | 55663 |
EuPathDBi | HostDB:ENSG00000083838.15 |
GeneCardsi | ZNF446 |
HGNCi | HGNC:21036, ZNF446 |
HPAi | ENSG00000083838, Low tissue specificity |
neXtProti | NX_Q9NWS9 |
OpenTargetsi | ENSG00000083838 |
PharmGKBi | PA134863395 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000162092 |
InParanoidi | Q9NWS9 |
OMAi | RRKPYTC |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q9NWS9 |
TreeFami | TF337489 |
Enzyme and pathway databases
PathwayCommonsi | Q9NWS9 |
Reactomei | R-HSA-212436, Generic Transcription Pathway |
Miscellaneous databases
BioGRID-ORCSi | 55663, 7 hits in 863 CRISPR screens |
ChiTaRSi | ZNF446, human |
GenomeRNAii | 55663 |
Pharosi | Q9NWS9, Tdark |
PROi | PR:Q9NWS9 |
RNActi | Q9NWS9, protein |
Gene expression databases
Bgeei | ENSG00000083838, Expressed in adenohypophysis and 158 other tissues |
ExpressionAtlasi | Q9NWS9, baseline and differential |
Genevisiblei | Q9NWS9, HS |
Family and domain databases
CDDi | cd07765, KRAB_A-box, 1 hit cd07936, SCAN, 1 hit |
Gene3Di | 1.10.4020.10, 1 hit |
InterProi | View protein in InterPro IPR001909, KRAB IPR036051, KRAB_dom_sf IPR003309, SCAN_dom IPR038269, SCAN_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF01352, KRAB, 1 hit PF02023, SCAN, 1 hit PF00096, zf-C2H2, 1 hit |
SMARTi | View protein in SMART SM00349, KRAB, 1 hit SM00431, SCAN, 1 hit SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF109640, SSF109640, 1 hit SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS50804, SCAN_BOX, 1 hit PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZN446_HUMAN | |
Accessioni | Q9NWS9Primary (citable) accession number: Q9NWS9 Secondary accession number(s): Q96AF5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 23, 2004 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - SIMILARITY comments
Index of protein domains and families - Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations