PAG1

Functionⁱ

Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.1 Publication

GO - Molecular functionⁱ

SH2 domain binding
Source: HGNC
Inferred from direct assayⁱ
- 10790433
SH3/SH2 adaptor activity
Source: HGNC
Non-traceable author statementⁱ
- 10790433

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

adaptive immune response Source: UniProtKB-KW
intracellular signal transduction
Source: HGNC
Inferred from direct assayⁱ
- 10790433
negative regulation of T cell activation
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
regulation of T cell activation
Source: ProtInc
Inferred from direct assayⁱ
- 10790433
signal transduction
Source: ProtInc
Traceable author statementⁱ
- 10790433
T cell receptor signaling pathway Source: Reactome

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Adaptive immunity, Immunity

Biological process

Adaptive immunity, Immunity

Enzyme and pathway databases

Reactomeⁱ	R-HSA-180292 GAB1 signalosome R-HSA-202427 Phosphorylation of CD3 and TCR zeta chains
SignaLinkⁱ	Q9NWQ8

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 Alternative name(s): Csk-binding protein Transmembrane adapter protein PAG Transmembrane phosphoprotein Cbp
Gene namesⁱ	Name:PAG1 Synonyms:CBP, PAG
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 8

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000076641.4
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:30043 PAG1
MIMⁱ	605767 gene
neXtProtⁱ	NX_Q9NWQ8

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 16	ExtracellularSequence analysisAdd BLAST	16
Transmembraneⁱ	17 – 37	Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST	21
Topological domainⁱ	38 – 432	CytoplasmicSequence analysisAdd BLAST	395

Keywords - Cellular componentⁱ

Cell membrane, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	105	Y → F: No effect on interaction with FYN or CSK. 1 Publication	1
Mutagenesisⁱ	163	Y → F: No effect on interaction with FYN or CSK. 1 Publication	1
Mutagenesisⁱ	181	Y → F: No effect on interaction with FYN or CSK. 1 Publication	1
Mutagenesisⁱ	227	Y → F: No effect on interaction with FYN or CSK. 1 Publication	1
Mutagenesisⁱ	299	Y → F: No effect on interaction with FYN or CSK. 1 Publication	1
Mutagenesisⁱ	317	Y → F: No effect on interaction with FYN. Abolishes interaction with CSK. 1 Publication	1
Mutagenesisⁱ	341	Y → F: No effect on interaction with FYN or CSK. 1 Publication	1
Mutagenesisⁱ	359	Y → F: No effect on interaction with FYN or CSK. 1 Publication	1
Mutagenesisⁱ	387	Y → F: No effect on interaction with FYN or CSK. 1 Publication	1
Mutagenesisⁱ	417	Y → F: No effect on interaction with FYN or CSK. 1 Publication	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	55824
Open Targets More...OpenTargetsⁱ	ENSG00000076641
PharmGKBⁱ	PA142671201

Polymorphism and mutation databases

BioMutaⁱ	PAG1
DMDMⁱ	84029384

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000083338	1 – 432	Phosphoprotein associated with glycosphingolipid-enriched microdomains 1Add BLAST		432

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Lipidationⁱ	37	S-palmitoyl cysteine1 Publication	1
Lipidationⁱ	40	S-palmitoyl cysteine1 Publication	1
Modified residueⁱ	50	PhosphoserineBy similarity	1
Modified residueⁱ	61	PhosphoserineBy similarity	1
Modified residueⁱ	105	Phosphotyrosine; by LYNBy similarity	1
Modified residueⁱ	163	PhosphotyrosineBy similarity	1
Modified residueⁱ	181	PhosphotyrosineBy similarity	1
Modified residueⁱ	227	PhosphotyrosineCombined sources	1
Modified residueⁱ	229	PhosphoserineCombined sources	1
Modified residueⁱ	317	Phosphotyrosine; by FYN and LYNCombined sources2 Publications	1
Modified residueⁱ	354	PhosphoserineBy similarity	1
Modified residueⁱ	359	PhosphotyrosineCombined sources	1
Modified residueⁱ	380	PhosphoserineBy similarity	1
Modified residueⁱ	387	PhosphotyrosineCombined sources	1
Modified residueⁱ	417	PhosphotyrosineCombined sources	1

Post-translational modificationⁱ

Palmitoylated.1 Publication

Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation. Phosphorylated by LYN.2 Publications

Keywords - PTMⁱ

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDⁱ	Q9NWQ8
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9NWQ8
PaxDbⁱ	Q9NWQ8
PeptideAtlas More...PeptideAtlasⁱ	Q9NWQ8
PRIDEⁱ	Q9NWQ8
ProteomicsDBⁱ	82961
TopDownProteomicsⁱ	Q9NWQ8

PTM databases

iPTMnetⁱ	Q9NWQ8
PhosphoSitePlusⁱ	Q9NWQ8
SwissPalmⁱ	Q9NWQ8

Expressionⁱ

Tissue specificityⁱ

Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).2 Publications

Gene expression databases

Bgeeⁱ	ENSG00000076641 Expressed in 204 organ(s), highest expression level in secondary oocyte
CleanExⁱ	HS_PAG1
Genevisibleⁱ	Q9NWQ8 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA001632 HPA066527

HPAⁱ

HPA001632
HPA066527

Interactionⁱ

Subunit structureⁱ

Interacts with FYN. When phosphorylated, interacts with CSK. Interacts with SLC9A3R1/EBP50. In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation. Interacts with LYN on plasma membrane lipid rafts. Identified in a complex with LYN and STAT3.3 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
CSK	P41240	3	EBI-2828115,EBI-1380630
FYN	P06241	5	EBI-2828115,EBI-515315
LYN	P07948	16	EBI-2828115,EBI-79452
RACK1	P63244	2	EBI-2828115,EBI-296739

GO - Molecular functionⁱ

SH2 domain binding
Source: HGNC
Inferred from direct assayⁱ
- 10790433
SH3/SH2 adaptor activity
Source: HGNC
Non-traceable author statementⁱ
- 10790433

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	120931, 42 interactors
IntActⁱ	Q9NWQ8, 11 interactors
Molecular INTeraction database More...MINTⁱ	Q9NWQ8
STRINGⁱ	9606.ENSP00000220597

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9NWQ8
SMRⁱ	Q9NWQ8
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	317 – 320	Interaction with CSK1 Publication		4
Regionⁱ	430 – 432	Interaction with SLC9A3R11 Publication		3

Keywords - Domainⁱ

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG410IFZ3 Eukaryota ENOG4111TW0 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000002061
HOGENOMⁱ	HOG000290651
HOVERGENⁱ	HBG055052
InParanoidⁱ	Q9NWQ8
OMAⁱ	PKCHQSR
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G09DV
PhylomeDBⁱ	Q9NWQ8
TreeFamⁱ	TF336170

Family and domain databases

InterProⁱ	View protein in InterPro IPR032748 PAG
PANTHERⁱ	PTHR16322 PTHR16322, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF15347 PAG, 1 hit
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD340439 PD340439, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9NWQ8-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MGPAGSLLGS GQMQITLWGS LAAVAIFFVI TFLIFLCSSC DREKKPRQHS 
        60         70         80         90        100
GDHENLMNVP SDKEMFSRSV TSLATDAPAS SEQNGALTNG DILSEDSTLT 
       110        120        130        140        150
CMQHYEEVQT SASDLLDSQD STGKPKCHQS RELPRIPPES AVDTMLTARS 
       160        170        180        190        200
VDGDQGLGME GPYEVLKDSS SQENMVEDCL YETVKEIKEV AAAAHLEKGH 
       210        220        230        240        250
SGKAKSTSAS KELPGPQTEG KAEFAEYASV DRNKKCRQSV NVESILGNSC 
       260        270        280        290        300
DPEEEAPPPV PVKLLDENEN LQEKEGGEAE ESATDTTSET NKRFSSLSYK 
       310        320        330        340        350
SREEDPTLTE EEISAMYSSV NKPGQLVNKS GQSLTVPEST YTSIQGDPQR 
       360        370        380        390        400
SPSSCNDLYA TVKDFEKTPN STLPPAGRPS EEPEPDYEAI QTLNREEEKA 
       410        420        430 
TLGTNGHHGL VPKENDYESI SDLQQGRDIT RL

Length:432

Mass (Da):46,981

Last modified:December 20, 2005 - v2

Checksum:ⁱE86272A0B7E3328C

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	4	A → V in AAH90931 (PubMed:15489334).Curated		1
Sequence conflictⁱ	36	L → P in BAA91321 (PubMed:14702039).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF240634 mRNA Translation: AAF67343.1 AK000680 mRNA Translation: BAA91321.1 AK289818 mRNA Translation: BAF82507.1 CH471068 Genomic DNA Translation: EAW87086.1 BC090931 mRNA Translation: AAH90931.1 BC112159 mRNA Translation: AAI12160.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS6227.1
NCBI Reference Sequences More...RefSeqⁱ	NP_060910.3, NM_018440.3 XP_016869129.1, XM_017013640.1 XP_016869130.1, XM_017013641.1 XP_016869131.1, XM_017013642.1 XP_016869132.1, XM_017013643.1
UniGeneⁱ	Hs.266175

Genome annotation databases

Ensemblⁱ	ENST00000220597; ENSP00000220597; ENSG00000076641
GeneIDⁱ	55824
KEGGⁱ	hsa:55824
UCSC genome browser More...UCSCⁱ	uc003ybz.4 human

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9NWQ8	phosphoprotein associated with glycosphingolipid-enriched microdomains 1		TARSY		435	UniRef90_Q9NWQ8
PAG1 isoform 1		PANTR		432
PAG1 isoform 1		PONAB		432
Uncharacterized protein		PANPA		432
Phosphoprotein membrane anchor with glycosphingolipid microdomains 1		GORGO		432
+1

Protein	Similar proteins		Species	Score	Length	Source
Q9NWQ8	Phosphoprotein associated with glycosphingolipid-enriched microdomains 1		MOUSE		429	UniRef50_Q9NWQ8
Phosphoprotein associated with glycosphingolipid-enriched microdomains 1	)	RAT		424
phosphoprotein associated with glycosphingolipid-enriched microdomains 1		TARSY		435
Phosphoprotein membrane anchor with glycosphingolipid microdomains 1		GORGO		432
PAG1 isoform 1		PONAB		432
+247

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF240634 mRNA Translation: AAF67343.1 AK000680 mRNA Translation: BAA91321.1 AK289818 mRNA Translation: BAF82507.1 CH471068 Genomic DNA Translation: EAW87086.1 BC090931 mRNA Translation: AAH90931.1 BC112159 mRNA Translation: AAI12160.1
CCDSⁱ	CCDS6227.1
RefSeqⁱ	NP_060910.3, NM_018440.3 XP_016869129.1, XM_017013640.1 XP_016869130.1, XM_017013641.1 XP_016869131.1, XM_017013642.1 XP_016869132.1, XM_017013643.1
UniGeneⁱ	Hs.266175

3D structure databases

ProteinModelPortalⁱ	Q9NWQ8
SMRⁱ	Q9NWQ8
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	120931, 42 interactors
IntActⁱ	Q9NWQ8, 11 interactors
MINTⁱ	Q9NWQ8
STRINGⁱ	9606.ENSP00000220597

PTM databases

iPTMnetⁱ	Q9NWQ8
PhosphoSitePlusⁱ	Q9NWQ8
SwissPalmⁱ	Q9NWQ8

Polymorphism and mutation databases

BioMutaⁱ	PAG1
DMDMⁱ	84029384

Proteomic databases

EPDⁱ	Q9NWQ8
MaxQBⁱ	Q9NWQ8
PaxDbⁱ	Q9NWQ8
PeptideAtlasⁱ	Q9NWQ8
PRIDEⁱ	Q9NWQ8
ProteomicsDBⁱ	82961
TopDownProteomicsⁱ	Q9NWQ8

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	55824
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000220597; ENSP00000220597; ENSG00000076641
GeneIDⁱ	55824
KEGGⁱ	hsa:55824
UCSCⁱ	uc003ybz.4 human

Organism-specific databases

CTDⁱ	55824
DisGeNETⁱ	55824
EuPathDBⁱ	HostDB:ENSG00000076641.4
GeneCardsⁱ	PAG1
HGNCⁱ	HGNC:30043 PAG1
HPAⁱ	HPA001632 HPA066527
MIMⁱ	605767 gene
neXtProtⁱ	NX_Q9NWQ8
OpenTargetsⁱ	ENSG00000076641
PharmGKBⁱ	PA142671201
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	ENOG410IFZ3 Eukaryota ENOG4111TW0 LUCA
GeneTreeⁱ	ENSGT00390000002061
HOGENOMⁱ	HOG000290651
HOVERGENⁱ	HBG055052
InParanoidⁱ	Q9NWQ8
OMAⁱ	PKCHQSR
OrthoDBⁱ	EOG091G09DV
PhylomeDBⁱ	Q9NWQ8
TreeFamⁱ	TF336170

Enzyme and pathway databases

Reactomeⁱ	R-HSA-180292 GAB1 signalosome R-HSA-202427 Phosphorylation of CD3 and TCR zeta chains
SignaLinkⁱ	Q9NWQ8

Miscellaneous databases

ChiTaRSⁱ	PAG1 human
GeneWikiⁱ	PAG1
GenomeRNAiⁱ	55824
Protein Ontology More...PROⁱ	PR:Q9NWQ8
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000076641 Expressed in 204 organ(s), highest expression level in secondary oocyte
CleanExⁱ	HS_PAG1
Genevisibleⁱ	Q9NWQ8 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR032748 PAG
PANTHERⁱ	PTHR16322 PTHR16322, 1 hit
Pfamⁱ	View protein in Pfam PF15347 PAG, 1 hit
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD340439 PD340439, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PHAG1_HUMAN
Accessionⁱ	Q9NWQ8Primary (citable) accession number: Q9NWQ8 Secondary accession number(s): A8K1A3 , Q2M1Z9, Q5BKU4, Q9NYK0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
	Last sequence update:	December 20, 2005
	Last modified:	November 7, 2018
	This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM

UniProtKB

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UniProtKB - Q9NWQ8 (PHAG1_HUMAN)

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Phosphoprotein associated with glycosphingolipid-enriched microdomains 1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ